Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,839,321 C→G 6.6% intergenic (‑57/‑203) ascG ← / → ascF asc operon transcriptional repressor; prpBC operon repressor/cellobiose/arbutin/salicin‑specific PTS enzymes, IIB and IC components

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,839,3210CG 6.6% 377.4 / 18.9 138intergenic (‑57/‑203)ascG/ascFasc operon transcriptional repressor; prpBC operon repressor/cellobiose/arbutin/salicin‑specific PTS enzymes, IIB and IC components
Reads supporting (aligned to +/- strand):  ref base C (63/64);  new base G (6/3);  total (71/67)
Fisher's exact test for biased strand distribution p-value = 4.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.08e-03

Only 100 of 140 total aligned reads displayed.

CTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCG  >  NC_000913/2839172‑2839469
                                                                                                                                                     |                                                                                                                                                    
ctcCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTc                                                                                                                                                      >  2:178129/1‑150 (MQ=255)
 tcCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTcc                                                                                                                                                     <  1:42229/150‑1 (MQ=255)
  ccTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAACCCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCCGTGTACTGCACCGTTTGCCGATTGTCCt                                                                                                                                                    >  1:622293/1‑150 (MQ=255)
  ccTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCt                                                                                                                                                    <  1:2101893/150‑1 (MQ=255)
  ccTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTAAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCt                                                                                                                                                    <  1:1500272/150‑1 (MQ=255)
   cTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACTGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCtt                                                                                                                                                   <  2:1432371/150‑1 (MQ=255)
     ggCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTTCACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGc                                                                                                                                                 >  1:724555/1‑150 (MQ=255)
     ggCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTACTTga                                                                                                                                                 >  2:157920/1‑149 (MQ=255)
     ggCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATAATCATCCCTTTACACGCATTCAACGGAGTGTACTGCACCGTTTGCCGATTGTCCTTGc                                                                                                                                                 >  2:1438107/1‑150 (MQ=255)
       cTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGcac                                                                                                                                               <  2:731315/150‑1 (MQ=255)
       cTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGcac                                                                                                                                               <  1:1563308/150‑1 (MQ=255)
             tAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATcgg                                                                                                                                         >  2:626980/1‑150 (MQ=255)
              aGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTGCAAGGACAATcggc                                                                                                                                        <  2:1789004/150‑1 (MQ=255)
              aGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTGCAAGGACAATcggc                                                                                                                                        <  2:1863975/150‑1 (MQ=255)
              aGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATcggc                                                                                                                                        >  2:1291433/1‑150 (MQ=255)
              aGCCATTACCTGAAAGCACGCGGGAAACGGGCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTGCAAGGACAATcggc                                                                                                                                        <  1:783175/150‑1 (MQ=255)
               gCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTTGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATcggcg                                                                                                                                       <  1:1853263/150‑1 (MQ=255)
               gCCATTACCTGAAAGCACGCGGGAAACGGTCGCGTTTGAAGCCCCGGCGCGCTTCGCCACTTCAAGCTTCGTAGTCAGCAATTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCGTGCACAATcggcg                                                                                                                                       >  2:410081/1‑150 (MQ=255)
                 cATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACCCGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACCATCGGCggg                                                                                                                                     >  1:1750632/1‑150 (MQ=255)
                 cATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCggg                                                                                                                                     <  1:1908736/150‑1 (MQ=255)
                    tACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCCCTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCATTCATCGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGtaa                                                                                                                                  >  1:67531/1‑150 (MQ=255)
                       cTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAat                                                                                                                               >  2:829823/1‑150 (MQ=255)
                       cTGAAAGCACGCGGGAAACGGGCGCTTTTGAAACCGCGGCGCGCTTCGACACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAat                                                                                                                               <  2:398180/150‑1 (MQ=255)
                        tGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAata                                                                                                                              >  1:1299074/1‑150 (MQ=255)
                            aGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCa                                                                                                                          <  1:1634253/150‑1 (MQ=255)
                             gCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCCCAATCGGCGGGAAAAATATTCAg                                                                                                                         >  2:11461/1‑150 (MQ=255)
                              cACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCGTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAgg                                                                                                                        >  2:1959227/1‑150 (MQ=255)
                                cgcgGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCCTCATTTTAATCCCGTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTg                                                                                                                      >  1:1960527/1‑150 (MQ=255)
                                 gcgGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGa                                                                                                                     <  2:175696/150‑1 (MQ=255)
                                  cgGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGAc                                                                                                                    <  2:1589585/150‑1 (MQ=255)
                                  cgGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGAAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGTCGGGAAAAATATTCAGGTTAc                                                                                                                    <  2:1247512/150‑1 (MQ=255)
                                           gTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTcac                                                                                                           <  1:2080066/150‑1 (MQ=255)
                                            tCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACCCGCAATCAACGCAGTGTACTGCACCGTTTGCCGTTTTTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTccca                                                                                                          >  1:1047383/1‑150 (MQ=255)
                                            tCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTcaca                                                                                                          <  2:1076587/150‑1 (MQ=255)
                                            tCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTcaca                                                                                                          <  2:2167778/150‑1 (MQ=255)
                                             cGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACaa                                                                                                         >  2:713793/1‑150 (MQ=255)
                                               cTTTTGAAACCCCGGCTCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTAATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAat                                                                                                       <  2:1605495/150‑1 (MQ=255)
                                               cTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAat                                                                                                       >  2:200238/1‑150 (MQ=255)
                                               cTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAat                                                                                                       <  1:178129/150‑1 (MQ=255)
                                               cTTTTGAAACCCCGGCGCGCTTCGCCACTCCCGCATCCTCGTCCTCATTTTAATCCCGTTACCCGGAATCCACCCAGTGTTCTGGCACGTTTGGCGCTTGTCCATTCACAATCGGCGGGAAAAATATTGAGGTTACCGGTTTAACaaaaa                                                                                                      >  2:419924/1‑148 (MQ=255)
                                                ttttGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCCATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAata                                                                                                      >  1:740516/1‑150 (MQ=255)
                                                  ttGAAACCCCGGCGCGCTTCGACACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAAtata                                                                                                    <  1:1343785/150‑1 (MQ=255)
                                                    gAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTAATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAatagata                                                                                                  >  1:1060756/1‑146 (MQ=255)
                                                       aCCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCGTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATaaaaaa                                                                                               >  2:2072556/1‑150 (MQ=255)
                                                         cccGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAATATATTCAGGTGACCGGTTTCAGAAATATATAAAATg                                                                                             >  2:1951681/1‑150 (MQ=255)
                                                         cccGGCGAGCTTCGCCACTTCCACCATCGTCGTCATCATTATCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATg                                                                                             <  2:100928/150‑1 (MQ=255)
                                                            ggCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAAc                                                                                          <  1:1118696/150‑1 (MQ=255)
                                                             gcgcgcTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACa                                                                                         <  1:1667682/150‑1 (MQ=255)
                                                                  cTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAAGCGGCGGGAAAAATATTCAGGTGCCCGGTTTCACAAATATAAAAAATGACCAATTCa                                                                                    >  2:1132694/1‑150 (MQ=255)
                                                                   ttCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCAc                                                                                   <  1:2194913/150‑1 (MQ=255)
                                                                     cGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCActc                                                                                 >  1:1894076/1‑150 (MQ=255)
                                                                      gCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTTCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCActct                                                                                >  1:1433224/1‑150 (MQ=255)
                                                                      gCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCActct                                                                                >  2:623980/1‑150 (MQ=255)
                                                                      gCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCActct                                                                                <  2:1377692/150‑1 (MQ=255)
                                                                       ccACTTCCAGCATCGTCGTCATCATTTTCAACCCTTTACACGCAATCAACGCAGAGTACTGCACCGTATCCCGATTGTCCTTGCACCATCGGCCGGAAAAATATTCAGGTGACCGGTTTCACCAATATAAAAAAAGAACAATTCActctc                                                                               >  1:1177599/1‑150 (MQ=255)
                                                                        cACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGATAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCActcgcg                                                                              >  2:2004753/1‑147 (MQ=255)
                                                                         aCTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCtt                                                                             <  1:638409/150‑1 (MQ=255)
                                                                            tCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGTGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCCCTCTCTTGCt                                                                          >  1:1078879/1‑150 (MQ=255)
                                                                                gCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGattatt                                                                      >  1:167752/1‑150 (MQ=255)
                                                                                   tcgtcgTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAg                                                                   <  2:1188990/150‑1 (MQ=255)
                                                                                       cgtcATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGAc                                                               <  2:1256507/150‑1 (MQ=255)
                                                                                              atTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTAtt                                                        >  2:250514/1‑150 (MQ=255)
                                                                                                tttCATGCCTTTACACGCAATCACCGCAGAGTTCTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCa                                                      <  2:2066277/150‑1 (MQ=255)
                                                                                                tttCATACCTTTACAAGCAATCAACGCAGTGTACTGCACCTTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAAAAATTCACTCTCTTGCTTATTTAGTGACAACTATTCa                                                      <  2:2074564/150‑1 (MQ=255)
                                                                                                      cccTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGAttt                                                <  1:623980/150‑1 (MQ=255)
                                                                                                         tttACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTtgt                                             >  1:855107/1‑150 (MQ=255)
                                                                                                          ttACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGAACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTtgtg                                            <  1:242232/150‑1 (MQ=255)
                                                                                                           tACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTAACAAATATAAAAAATGAACAATACACTCTGTTGCTTATTTAGTGACAACTATTCATGATTTTGTGa                                           >  1:145932/1‑150 (MQ=255)
                                                                                                            acacGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGaa                                          >  2:1587482/1‑150 (MQ=255)
                                                                                                               cGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAAcc                                       >  1:2088679/1‑150 (MQ=255)
                                                                                                               cGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACACTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAAcc                                       >  1:143739/1‑150 (MQ=255)
                                                                                                                   aTCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGtt                                   <  1:763651/150‑1 (MQ=255)
                                                                                                                    tCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGttt                                  <  1:296831/150‑1 (MQ=255)
                                                                                                                     cAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATTTTCAGGTGACCGTTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTc                                 <  2:259904/150‑1 (MQ=255)
                                                                                                                     cAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTc                                 <  2:1843351/150‑1 (MQ=255)
                                                                                                                     cAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTc                                 <  2:2180723/150‑1 (MQ=255)
                                                                                                                     cAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCAGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTc                                 <  1:37868/150‑1 (MQ=255)
                                                                                                                      aaCGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGTAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCt                                <  1:1387409/150‑1 (MQ=255)
                                                                                                                      aaCGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCt                                >  1:2006103/1‑150 (MQ=255)
                                                                                                                      aaCGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTATCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCt                                >  1:1100216/1‑150 (MQ=255)
                                                                                                                      aaCGCAGTGTACTGCACCGTTTGCCCATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGAGACAAGTATTCATGATTTTGTGAAACCGGtttgt                                >  2:27419/1‑148 (MQ=255)
                                                                                                                        cGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTa                              <  2:724555/150‑1 (MQ=255)
                                                                                                                            gtgtACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTc                          >  2:616088/1‑150 (MQ=255)
                                                                                                                                    cACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAg                  <  1:89666/150‑1 (MQ=255)
                                                                                                                                      ccGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCa                <  1:1181037/150‑1 (MQ=255)
                                                                                                                                      ccGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTAACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTGATTCCTTTtcatca                >  2:316480/1‑150 (MQ=255)
                                                                                                                                         tttGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCg             <  2:1618802/150‑1 (MQ=255)
                                                                                                                                           tGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGc           >  2:1461167/1‑150 (MQ=255)
                                                                                                                                           tGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGc           >  1:823458/1‑150 (MQ=255)
                                                                                                                                           cgCCGATTGTGCAAGGACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCAGc           >  1:296552/2‑150 (MQ=255)
                                                                                                                                            gCCGATTGTGCAAGGACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTGTTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCa          >  2:837981/1‑150 (MQ=255)
                                                                                                                                            gCCGATTGTGCAAGGACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCa          >  2:619839/1‑150 (MQ=255)
                                                                                                                                            gCCGATTGTGCAAGGACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCa          >  2:2214835/1‑150 (MQ=255)
                                                                                                                                            gCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCa          >  1:520459/1‑150 (MQ=255)
                                                                                                                                            gCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGATTCACAAATAAAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAAATCCGTTTCAGCATCGGCa          <  2:67531/150‑1 (MQ=255)
                                                                                                                                               gATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCAttt       >  2:702120/1‑150 (MQ=255)
                                                                                                                                                 ttGTCCTTGCAAAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCAttttt     <  1:856969/150‑1 (MQ=255)
                                                                                                                                                   gTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTcc   <  1:26259/150‑1 (MQ=255)
                                                                                                                                                    tCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCg  <  2:622293/150‑1 (MQ=255)
                                                                                                                                                    tCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCAAAAATATAAAAAATTAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATTATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCg  <  2:1894076/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CTCCTGGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCG  >  NC_000913/2839172‑2839469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: