Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,839,326 C→G 6.8% intergenic (‑62/‑198) ascG ← / → ascF asc operon transcriptional repressor; prpBC operon repressor/cellobiose/arbutin/salicin‑specific PTS enzymes, IIB and IC components

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,839,3260CG 6.8% 376.4 / 19.0 133intergenic (‑62/‑198)ascG/ascFasc operon transcriptional repressor; prpBC operon repressor/cellobiose/arbutin/salicin‑specific PTS enzymes, IIB and IC components
Reads supporting (aligned to +/- strand):  ref base C (64/58);  new base G (6/3);  total (70/63)
Fisher's exact test for biased strand distribution p-value = 5.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.23e-02

Only 100 of 138 total aligned reads displayed.

GGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCA  >  NC_000913/2839177‑2839472
                                                                                                                                                     |                                                                                                                                                  
ggCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTTCACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGc                                                                                                                                                    >  1:724555/1‑150 (MQ=255)
ggCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATAATCATCCCTTTACACGCATTCAACGGAGTGTACTGCACCGTTTGCCGATTGTCCTTGc                                                                                                                                                    >  2:1438107/1‑150 (MQ=255)
  cTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGcac                                                                                                                                                  <  2:731315/150‑1 (MQ=255)
  cTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGcac                                                                                                                                                  <  1:1563308/150‑1 (MQ=255)
        tAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATcgg                                                                                                                                            >  2:626980/1‑150 (MQ=255)
         aGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTGCAAGGACAATcggc                                                                                                                                           <  2:1863975/150‑1 (MQ=255)
         aGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATcggc                                                                                                                                           >  2:1291433/1‑150 (MQ=255)
         aGCCATTACCTGAAAGCACGCGGGAAACGGGCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTGCAAGGACAATcggc                                                                                                                                           <  1:783175/150‑1 (MQ=255)
          gCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTTGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATcggcg                                                                                                                                          <  1:1853263/150‑1 (MQ=255)
          gCCATTACCTGAAAGCACGCGGGAAACGGTCGCGTTTGAAGCCCCGGCGCGCTTCGCCACTTCAAGCTTCGTAGTCAGCAATTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCGTGCACAATcggcg                                                                                                                                          >  2:410081/1‑150 (MQ=255)
            cATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACCCGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACCATCGGCggg                                                                                                                                        >  1:1750632/1‑150 (MQ=255)
            cATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCggg                                                                                                                                        <  2:711921/150‑1 (MQ=255)
               tACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCCCTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCATTCATCGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGtaa                                                                                                                                     >  1:67531/1‑150 (MQ=255)
                  cTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAat                                                                                                                                  >  2:829823/1‑150 (MQ=255)
                       aGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCa                                                                                                                             <  2:361326/150‑1 (MQ=255)
                       aGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCa                                                                                                                             <  1:1634253/150‑1 (MQ=255)
                       aGCACGCGGGAAACGGTCGCTTTTGAAACACCGGCGCGCTTCGCCACTTCCAGCATCGTCTTCATCATTTTCATCCCTTTACATGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCa                                                                                                                             <  1:247123/150‑1 (MQ=255)
                        gCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCCCAATCGGCGGGAAAAATATTCAg                                                                                                                            >  2:11461/1‑150 (MQ=255)
                         cACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCGTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAgg                                                                                                                           >  2:1959227/1‑150 (MQ=255)
                           cgcgGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCCTCATTTTAATCCCGTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTg                                                                                                                         >  1:1960527/1‑150 (MQ=255)
                            gcgGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGa                                                                                                                        <  2:175696/150‑1 (MQ=255)
                             cgGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGAc                                                                                                                       <  2:1589585/150‑1 (MQ=255)
                             cgGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGAAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGTCGGGAAAAATATTCAGGTTAc                                                                                                                       <  2:1247512/150‑1 (MQ=255)
                              gggAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGAcc                                                                                                                      <  2:64770/150‑1 (MQ=255)
                                    cGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGGGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTc                                                                                                                <  1:1333648/150‑1 (MQ=255)
                                       tCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACCCGCAATCAACGCAGTGTACTGCACCGTTTGCCGTTTTTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTccca                                                                                                             >  1:1047383/1‑150 (MQ=255)
                                       tCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTcaca                                                                                                             <  2:2167778/150‑1 (MQ=255)
                                         gCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACaaa                                                                                                           <  1:2009971/150‑1 (MQ=255)
                                         gCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCAATTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGAGACCGGTTTCACaaa                                                                                                           >  2:621326/1‑150 (MQ=255)
                                          cTTTTGAAACCCCGGCTCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTAATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAat                                                                                                          <  2:1605495/150‑1 (MQ=255)
                                          cTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAat                                                                                                          <  1:178129/150‑1 (MQ=255)
                                          cTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAat                                                                                                          >  2:200238/1‑150 (MQ=255)
                                          cTTTTGAAACCCCGGCGCGCTTCGCCACTCCCGCATCCTCGTCCTCATTTTAATCCCGTTACCCGGAATCCACCCAGTGTTCTGGCACGTTTGGCGCTTGTCCATTCACAATCGGCGGGAAAAATATTGAGGTTACCGGTTTAACaaaaa                                                                                                         >  2:419924/1‑148 (MQ=255)
                                           ttttGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCCATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAata                                                                                                         >  1:740516/1‑150 (MQ=255)
                                             ttGAAACCCCGGCGCGCTTCGACACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAAtata                                                                                                       <  1:1343785/150‑1 (MQ=255)
                                               gAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTAATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAatagata                                                                                                     >  1:1060756/1‑146 (MQ=255)
                                                 aaCCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCCTCCCTTTACACGCAATCAACGCAGTGTACTGCCCCGTTTTCCGTTTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCTCAAATATaaaaa                                                                                                   >  1:1735775/1‑150 (MQ=255)
                                                  aCCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCGTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATaaaaaa                                                                                                  >  2:2072556/1‑150 (MQ=255)
                                                  aCCCCGGCGCGCTTCGCCACTTCCACCATCGGCGTCCAGATTTTAATACCTTTACAACCAATCAACCCAGTGTAGTGCACCGGTTGCCGATTGTAAATACAAACTCCGCGGGAAAAATTTTCAGGTGACCCGCTTCACAAAATTaataaa                                                                                                  >  2:1128330/1‑150 (MQ=255)
                                                    cccGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAATATATTCAGGTGACCGGTTTCAGAAATATATAAAATg                                                                                                >  2:1951681/1‑150 (MQ=255)
                                                    cccGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATg                                                                                                <  1:949217/150‑1 (MQ=255)
                                                    cccGGCGAGCTTCGCCACTTCCACCATCGTCGTCATCATTATCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATg                                                                                                <  2:100928/150‑1 (MQ=255)
                                                       ggCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAAc                                                                                             <  1:1118696/150‑1 (MQ=255)
                                                        gcgcgcTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACa                                                                                            <  1:1667682/150‑1 (MQ=255)
                                                           cgcTTCGCCACTTCCAGCATCGTCTTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATGCAGGTGACCGGTTTCACAAATATAAAAAATGAACAAtt                                                                                         <  1:372346/150‑1 (MQ=255)
                                                             cTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAAGCGGCGGGAAAAATATTCAGGTGCCCGGTTTCACAAATATAAAAAATGACCAATTCa                                                                                       >  2:1132694/1‑150 (MQ=255)
                                                                cGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCActc                                                                                    >  1:1894076/1‑150 (MQ=255)
                                                                 gCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTTCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCActct                                                                                   >  1:1433224/1‑150 (MQ=255)
                                                                 gCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCActct                                                                                   >  2:1561987/1‑150 (MQ=255)
                                                                 gCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCActct                                                                                   <  2:1377692/150‑1 (MQ=255)
                                                                 gCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCActct                                                                                   >  2:623980/1‑150 (MQ=255)
                                                                  ccACTTCCAGCATCGTCGTCATCATTTTCAACCCTTTACACGCAATCAACGCAGAGTACTGCACCGTATCCCGATTGTCCTTGCACCATCGGCCGGAAAAATATTCAGGTGACCGGTTTCACCAATATAAAAAAAGAACAATTCActctc                                                                                  >  1:1177599/1‑150 (MQ=255)
                                                                   cACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTTCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCActctct                                                                                 <  1:2116733/150‑1 (MQ=255)
                                                                   cACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGATAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCActcgcg                                                                                 >  2:2004753/1‑147 (MQ=255)
                                                                    aCTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCtt                                                                                <  1:638409/150‑1 (MQ=255)
                                                                                  cgtcATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGAc                                                                  <  2:1256507/150‑1 (MQ=255)
                                                                                     catcatTTTCATCCCTTTACACGCAATCAACGCATTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAAc                                                               <  1:157920/150‑1 (MQ=255)
                                                                                      atcatTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCATTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAAGAATTCACTCTCTTGCTTATTTAGTGACAACt                                                              >  1:1079153/1‑150 (MQ=255)
                                                                                         atTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTAtt                                                           >  2:250514/1‑150 (MQ=255)
                                                                                           tttCATGCCTTTACACGCAATCACCGCAGAGTTCTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCa                                                         <  2:2066277/150‑1 (MQ=255)
                                                                                           tttCATACCTTTACAAGCAATCAACGCAGTGTACTGCACCTTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAAAAATTCACTCTCTTGCTTATTTAGTGACAACTATTCa                                                         <  2:2074564/150‑1 (MQ=255)
                                                                                                 cccTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACTAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGAttt                                                   >  2:169821/1‑150 (MQ=255)
                                                                                                 cccTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGAttt                                                   <  1:623980/150‑1 (MQ=255)
                                                                                                  ccTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGAtttt                                                  >  1:633996/1‑150 (MQ=255)
                                                                                                    tttACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTtgt                                                >  1:855107/1‑150 (MQ=255)
                                                                                                     ttACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGAACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTtgtg                                               <  1:242232/150‑1 (MQ=255)
                                                                                                      tACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTAACAAATATAAAAAATGAACAATACACTCTGTTGCTTATTTAGTGACAACTATTCATGATTTTGTGa                                              >  1:145932/1‑150 (MQ=255)
                                                                                                       acacGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATAAACTATCTTGCTAATTTAGTGACAACTATTCATGATTTTGTGaa                                             >  2:1595214/1‑150 (MQ=255)
                                                                                                          cGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAAcc                                          >  1:2088679/1‑150 (MQ=255)
                                                                                                          cGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAAcc                                          >  2:1230466/1‑150 (MQ=255)
                                                                                                          cGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACACTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAAcc                                          >  1:143739/1‑150 (MQ=255)
                                                                                                             aaTCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGt                                       <  1:844201/150‑1 (MQ=255)
                                                                                                               tCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGttt                                     <  1:296831/150‑1 (MQ=255)
                                                                                                                cAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATTTTCAGGTGACCGTTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTc                                    <  2:259904/150‑1 (MQ=255)
                                                                                                                cAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTc                                    <  2:1843351/150‑1 (MQ=255)
                                                                                                                cAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTc                                    <  2:2180723/150‑1 (MQ=255)
                                                                                                                cAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCAGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTc                                    <  1:37868/150‑1 (MQ=255)
                                                                                                                 aaCGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGTAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCt                                   <  1:1387409/150‑1 (MQ=255)
                                                                                                                 aaCGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCt                                   >  1:2006103/1‑150 (MQ=255)
                                                                                                                 aaCGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTATCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCt                                   >  1:1100216/1‑150 (MQ=255)
                                                                                                                 aaCGCAGTGTACTGCACCGTTTGCCCATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGAGACAAGTATTCATGATTTTGTGAAACCGGtttgt                                   >  2:27419/1‑148 (MQ=255)
                                                                                                                   cGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTa                                 <  2:724555/150‑1 (MQ=255)
                                                                                                                   cGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTa                                 <  1:78160/150‑1 (MQ=255)
                                                                                                                       gtgtACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTc                             >  2:616088/1‑150 (MQ=255)
                                                                                                                                 ccGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCa                   <  1:1181037/150‑1 (MQ=255)
                                                                                                                                 ccGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTAACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTGATTCCTTTtcatca                   >  2:316480/1‑150 (MQ=255)
                                                                                                                                    cccgCCGATTGTGCAAGGACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCg                >  2:1824101/4‑150 (MQ=255)
                                                                                                                                      tGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGc              >  2:1461167/1‑150 (MQ=255)
                                                                                                                                       gCCGATTGTGCAAGGACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTGTTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCa             >  2:837981/1‑150 (MQ=255)
                                                                                                                                       gCCGATTGTGCAAGGACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCa             >  2:2214835/1‑150 (MQ=255)
                                                                                                                                       gCCGATTGTGCAAGGACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCa             >  2:619839/1‑150 (MQ=255)
                                                                                                                                       gCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCa             >  1:520459/1‑150 (MQ=255)
                                                                                                                                          gATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCAttt          >  2:702120/1‑150 (MQ=255)
                                                                                                                                            ttGTCCTTGCAAAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCAttttt        <  1:856969/150‑1 (MQ=255)
                                                                                                                                              gTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTcc      <  1:26259/150‑1 (MQ=255)
                                                                                                                                               tCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCAAAAATATAAAAAATTAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATTATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCg     <  2:1894076/150‑1 (MQ=255)
                                                                                                                                                 cTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGt  >  1:1649313/1‑149 (MQ=255)
                                                                                                                                                 cTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTc   >  2:730064/1‑150 (MQ=255)
                                                                                                                                                 cTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTc   >  1:712337/1‑150 (MQ=255)
                                                                                                                                                  ttGCACAATCGGCGTGAAAAATATTCAGGTGACCGGTTTCACAAATATTAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCa  <  1:1007319/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
GGCTGACGTAGCCATTACCTGAAAGCACGCGGGAAACGGTCGCTTTTGAAACCCCGGCGCGCTTCGCCACTTCCAGCATCGTCGTCATCATTTTCATCCCTTTACACGCAATCAACGCAGTGTACTGCACCGTTTGCCGATTGTCCTTGCACAATCGGCGGGAAAAATATTCAGGTGACCGGTTTCACAAATATAAAAAATGAACAATTCACTCTCTTGCTTATTTAGTGACAACTATTCATGATTTTGTGAAACCGGTTTCTTAATTCCGTTTCAGCATCGGCATTTTTCCGTCA  >  NC_000913/2839177‑2839472

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: