Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,884,578 C→A 5.6% R881I (AGA→ATA)  ygcB ← Cascade complex anti‑viral R‑loop helicase‑annealase Cas3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,884,5780CA 5.6% 520.3 / 12.2 171R881I (AGA→ATA) ygcBCascade complex anti‑viral R‑loop helicase‑annealase Cas3
Reads supporting (aligned to +/- strand):  ref base C (79/81);  new base A (6/5);  total (85/86)
Fisher's exact test for biased strand distribution p-value = 7.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 177 total aligned reads displayed.

TGTGAATATAAAATCTCATACCGGGAAATTAAAAGAAGATGTACATTGTGCACCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATT  >  NC_000913/2884430‑2884726
                                                                                                                                                    |                                                                                                                                                    
tgtgAATATAAAATCTCATACCGGGAAATTAATAGAAGATGTACATAGTGCACCTTCCCTACTTAAGTTGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGActct                                                                                                                                                     <  2:249963/150‑1 (MQ=255)
tgtgAATATAAAATCTCATACCGGGAAATTAAAAGAAGATGTACATTGTGCACCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGActct                                                                                                                                                     <  1:990040/150‑1 (MQ=255)
 gtgAATATAAAATCTCATACCGGGAAATTAAAAGAAGATGTACATTGTGCACCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTTATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTg                                                                                                                                                    >  1:1394363/1‑150 (MQ=255)
 gtgAATATAAAATCTCATACCGGGAAATTAAAAGAAGATGTACATTGTGCACCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTg                                                                                                                                                    >  2:1057411/1‑150 (MQ=255)
  tgAATATAAAATCTCATACCGGGAAATTAAAAGAAGATGTACATTGTGCACCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTgg                                                                                                                                                   <  1:2005839/150‑1 (MQ=255)
  tgAATATAAAATCTCATACCGGGAAATTAAAAGAAGATGTACATTGTGCACCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTgg                                                                                                                                                   <  1:1813354/150‑1 (MQ=255)
   gAATATAAAATCTCATACCGGGAAATTAAAAGAAGATGTACATTGTGCACCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGt                                                                                                                                                  <  1:2187991/150‑1 (MQ=255)
    aaTATAAAATCTCATACCGGGAAATAAAAAGAAGATGTACATTGTGCACCTTCCCTACATAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGAAAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTc                                                                                                                                                 <  1:358488/150‑1 (MQ=255)
      tataAAATCTCATACCGGGAAATTAAAAGAAGATGTACATTGTGCACCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCAGGTCAt                                                                                                                                               >  1:2060978/1‑150 (MQ=255)
         aaaaTCTCATACCGGGAAATTAAAAGAAGATGTACATTGTGCACCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATccc                                                                                                                                            <  2:1031721/150‑1 (MQ=255)
         aaaaTCTCATACCGGGAAATTAAAAGAAGATGTACATTGTGCACCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATccc                                                                                                                                            >  2:173099/1‑150 (MQ=255)
          aaaacacATACCGGGAAATTAAAAGAAGATGTACATTGTGCAAATTCCCTACAAAAGTAGCGATAAACCCTAATTGGACTTATTATCGTCAATGAAAACAATCATTCCCTAAGTTATTTCGGATTTGCATGGATCAATATGGTCATCCCt                                                                                                                                           >  1:1518812/7‑150 (MQ=255)
           aaTCTCATACCGGGAAATTAAAAGAAGATGTACATTGTGCACCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACCAGAGTCATCCCtg                                                                                                                                          <  1:1871310/150‑2 (MQ=255)
            aTCTCATACCGGGAAATTAACAGAAGATGTACATTGTGAACCTTCCCTACTTAAGTAGCGATAAACCGATATAGGTCTTATAAACGTCATTCATAACAATCATTCGCGATGTTATTTGGGATTAGCAGGCATGAGACTGCTCATCCCATc                                                                                                                                         >  1:126793/1‑150 (MQ=255)
            aTCTCATACCGGGAAATCAAAAGAAGATGTACATTGTGCACCTTCCCTACTTACGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAAAAATCATTCCCGAAGGTTTTTGGGATTTGCAGGGATTACCAGAGTCATCCCtgc                                                                                                                                         <  2:581763/150‑3 (MQ=255)
                    ccGGGATATTAAAAGAAGATGTACATTGTGCACCTTCCCTACTTAAGTAGGGATATACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATACCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATACCCTg                                                                                                                                 >  2:329000/1‑150 (MQ=255)
                    ccGGGAAATTAAAAGAAGATGTACATTGTGCACCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTg                                                                                                                                 <  2:548708/150‑1 (MQ=255)
                    ccGGGAAATTAAAAGAAGATGTACATTGTGCACCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTg                                                                                                                                 >  1:550186/1‑150 (MQ=255)
                    ccGGGAAATTAAAAGAAGATGTACATTGTGCACCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTg                                                                                                                                 <  1:389503/150‑1 (MQ=255)
                    ccGGGAAATTAAAAGAAGATGTACATTGTGCACCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCAGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCACCTg                                                                                                                                 >  1:637029/1‑150 (MQ=255)
                     cGGGAAATTAAAAGAAGATGTACATTGTGCACCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTCATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTGATCCCCTGt                                                                                                                                >  2:413763/1‑150 (MQ=255)
                        taaaTTAAAAGAAGATGTACATTGTGAACCTTCACTACTTTAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGtata                                                                                                                             <  1:324493/149‑1 (MQ=255)
                        gAAATTAAAAGAAGATGTACATTGTGCACCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGtata                                                                                                                             <  1:1607996/150‑1 (MQ=255)
                        gAAATTAAAAGAAGATGTACATTGTGCACCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGtata                                                                                                                             <  2:1757979/150‑1 (MQ=255)
                          aaTTAAAAGAAGATGTACATTGTGCACCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAgg                                                                                                                           <  1:952334/150‑1 (MQ=255)
                           aTTAAAAGAAGATGTACATTGTGCACCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCTTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGt                                                                                                                          >  2:1387670/1‑150 (MQ=255)
                             tAAAAGAAGATGTACATTGTGCACCTTCCCTACTTAAGTATGGATAAACAGTTATTGGTCTTATTATCGTCATTGATAACAATAATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGtaa                                                                                                                        >  2:856741/1‑150 (MQ=255)
                              aaaaGAAGATGTACATTGTGCACCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGtaat                                                                                                                       <  1:1096194/150‑1 (MQ=255)
                                aagaagATGTACATTGTGCACCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCATGGATGACTCTGGTCATCCCTTCATACACTGTATAGGtaataa                                                                                                                     >  1:175941/1‑150 (MQ=255)
                                 agaagaTGTACATTGTGCACCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAAc                                                                                                                    <  1:1898546/150‑1 (MQ=255)
                                  gaagaTGTACATTGTGCACCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCACCTGTATAGGTAATAACa                                                                                                                   >  1:1357092/1‑150 (MQ=255)
                                        gTACATTGTGCACCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTg                                                                                                             >  2:2114641/1‑150 (MQ=255)
                                        gTACATTGTGCACCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTg                                                                                                             >  2:1817585/1‑150 (MQ=255)
                                        gTACATTGTGCACCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTg                                                                                                             <  2:686078/150‑1 (MQ=255)
                                        gTACATTGTGCACCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATACCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTg                                                                                                             <  1:1181371/150‑1 (MQ=255)
                                         tACATTGTGCACCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCGTTCATCCCCTGTATAGGTAATAACATAACTGt                                                                                                            >  1:919338/1‑150 (MQ=255)
                                         tACATTGTGCACCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGt                                                                                                            >  1:197412/1‑150 (MQ=255)
                                          aCATTGTGCACCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTGATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGACAGCCCTTCATCCCCTGTATAGGTAATAACAATACTGtt                                                                                                           >  2:2019868/1‑150 (MQ=255)
                                            aTTGTGCACCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTAc                                                                                                         <  1:498140/150‑1 (MQ=255)
                                             ttGTGCACCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTAcc                                                                                                        >  2:1318768/1‑150 (MQ=255)
                                              tgtgCACCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTAccc                                                                                                       <  2:2027826/150‑1 (MQ=255)
                                                 gCACCTTCCCTACTTAAGTATGGATAAACCGTTATTGGTCTTATTATAGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGc                                                                                                    <  1:1227534/150‑1 (MQ=255)
                                                  cACCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGcc                                                                                                   <  1:36695/150‑1 (MQ=255)
                                                  cACCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGcc                                                                                                   <  1:1458861/150‑1 (MQ=255)
                                                   aCCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCa                                                                                                  <  1:371917/150‑1 (MQ=255)
                                                   aCCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCa                                                                                                  <  1:1576507/150‑1 (MQ=255)
                                                    ccTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTCCCCTGCCAg                                                                                                 >  1:129682/1‑150 (MQ=255)
                                                        cccTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGAccc                                                                                             >  2:1723739/1‑150 (MQ=255)
                                                           tACATAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCATTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACccatc                                                                                          >  2:101192/1‑150 (MQ=255)
                                                            aCTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACccatcc                                                                                         <  2:1612168/150‑1 (MQ=255)
                                                                 aGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCAtccatcca                                                                                    <  1:1399924/150‑1 (MQ=255)
                                                                   tAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGa                                                                                  >  1:415119/1‑150 (MQ=255)
                                                                    aGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGTATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGAt                                                                                 <  1:1302118/150‑1 (MQ=255)
                                                                      ggATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTc                                                                               <  2:1563309/150‑1 (MQ=255)
                                                                         tAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGt                                                                            <  2:1885069/150‑1 (MQ=255)
                                                                          aaaCCGTTATTGGTCTTATTATCGTCATTTATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGtt                                                                           <  1:1003129/150‑1 (MQ=255)
                                                                          aaaCCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGtt                                                                           >  2:710084/1‑150 (MQ=255)
                                                                           aaCCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGttt                                                                          >  1:954623/1‑150 (MQ=255)
                                                                           aaCCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCATTCATCCCCTGTATAGGTAATAACAATACTGTTACCATGCCAGACCCATCCATCCAGATTCTGttt                                                                          >  1:1431933/1‑150 (MQ=255)
                                                                            aCCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTc                                                                         >  2:1097801/1‑150 (MQ=255)
                                                                             ccGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGTGATGACTCTGGTCATCACTTCATCCCCTTTATAGGTAATAACAATACTCTTAAACTGCCAGACCCATCCATCCAGATTCTCTTTcc                                                                        >  1:304525/1‑150 (MQ=255)
                                                                             ccGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTcc                                                                        <  1:1723739/150‑1 (MQ=255)
                                                                             ccGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTcc                                                                        <  1:1308148/150‑1 (MQ=255)
                                                                               gTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCt                                                                      >  2:38410/1‑150 (MQ=255)
                                                                                 tATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTc                                                                    <  2:979121/150‑1 (MQ=255)
                                                                                 tATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTc                                                                    <  2:1482086/150‑1 (MQ=255)
                                                                                  aTTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTcc                                                                   <  1:1824863/150‑1 (MQ=255)
                                                                                  aTTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTACCTTcc                                                                   >  2:1078514/1‑150 (MQ=255)
                                                                                    tGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTCTTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAg                                                                 <  2:47025/150‑1 (MQ=255)
                                                                                     ggTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATTGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCACTTCCAGc                                                                >  1:114959/1‑150 (MQ=255)
                                                                                     ggTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGc                                                                >  2:2052546/1‑150 (MQ=255)
                                                                                      gTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGcc                                                               >  1:588067/1‑150 (MQ=255)
                                                                                          tattatCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAg                                                           >  1:2184862/1‑150 (MQ=255)
                                                                                           attatCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGt                                                          <  2:1326568/150‑1 (MQ=255)
                                                                                           attatCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGt                                                          <  1:249166/150‑1 (MQ=255)
                                                                                            ttatCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTTCCAGACCCATCCATCCAGAATCTGATTCCCTTCCAGCCAAAGTa                                                         >  2:769523/1‑150 (MQ=255)
                                                                                              atCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAAc                                                       >  2:969976/1‑150 (MQ=255)
                                                                                                 gTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCa                                                    <  2:469891/150‑1 (MQ=255)
                                                                                                  tCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCAt                                                   >  1:1948743/1‑150 (MQ=255)
                                                                                                       taTAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTATGGTCATCCCTTCATACCCTGTATATGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCa                                              <  2:850070/149‑1 (MQ=255)
                                                                                                       gATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACATTACTGTTACACTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCa                                              >  1:1398677/1‑150 (MQ=255)
                                                                                                         tAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATc                                            <  1:173099/150‑1 (MQ=255)
                                                                                                         tAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATc                                            >  1:1049768/1‑150 (MQ=255)
                                                                                                                  ttCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTc                                   <  1:102713/150‑1 (MQ=255)
                                                                                                                   tCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCa                                  >  2:1143862/1‑150 (MQ=255)
                                                                                                                    cccGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAg                                 <  2:2067659/150‑1 (MQ=255)
                                                                                                                    cccGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAg                                 <  2:1852742/150‑1 (MQ=255)
                                                                                                                    cccGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAg                                 <  2:830528/150‑1 (MQ=255)
                                                                                                                      cGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAGaa                               >  1:613067/1‑150 (MQ=255)
                                                                                                                               ttGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGt                      >  1:1793020/1‑150 (MQ=255)
                                                                                                                                    aTTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCa                 <  2:197412/150‑1 (MQ=255)
                                                                                                                                      ttGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAgg               <  2:628511/150‑1 (MQ=255)
                                                                                                                                       tGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGt              >  1:819576/1‑150 (MQ=255)
                                                                                                                                        gaaGGGATGACCAGAGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTTCTTCAGAAAAACTACGTTTCCAGGTg             >  1:1701311/3‑150 (MQ=255)
                                                                                                                                            ggATGACGCTGGTCATCCCTGCATTCCCTGTATAGGTAATAACAATACTGTTACCCGGCCAGACCCATCCATCCAGATTCTGTTTCCCGGCGCGCCAAAGTACGCAATCTTCATCTACTAGTGCAGAAAAACTACGTGTCCAGCTGCAgg         >  2:146473/1‑150 (MQ=255)
                                                                                                                                             gATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAggg        >  1:1829198/1‑150 (MQ=255)
                                                                                                                                              aTGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGt       <  2:2202811/150‑1 (MQ=255)
                                                                                                                                                gACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTAc     <  2:920521/150‑1 (MQ=255)
                                                                                                                                                  ctctGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCATCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACAt   >  2:1345860/1‑150 (MQ=255)
                                                                                                                                                   tctGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACAtt  <  2:1334639/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TGTGAATATAAAATCTCATACCGGGAAATTAAAAGAAGATGTACATTGTGCACCTTCCCTACTTAAGTAGGGATAAACCGTTATTGGTCTTATTATCGTCATTGATAACAATCATTCCCGAAGTTATTTGGGATTTGCAGGGATGACTCTGGTCATCCCTTCATCCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATT  >  NC_000913/2884430‑2884726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: