Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,906,607 C→T 7.2% intergenic (+24/+36) ygcG → / ← eno TPM domain protein, putative phosphatase/enolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,906,6070CT 7.2% 525.8 / 18.7 169intergenic (+24/+36)ygcG/enoTPM domain protein, putative phosphatase/enolase
Reads supporting (aligned to +/- strand):  ref base C (67/89);  new base T (6/6);  total (74/95)
Fisher's exact test for biased strand distribution p-value = 7.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.44e-01

Only 100 of 175 total aligned reads displayed.

CAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGA  >  NC_000913/2906458‑2906756
                                                                                                                                                     |                                                                                                                                                     
cAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGccc                                                                                                                                                       >  1:851113/1‑150 (MQ=255)
   aaaaGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGa                                                                                                                                                    <  2:862715/150‑1 (MQ=255)
         gCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGATGCTCAGATAGCGGCGGATTTAGCCGCGGGGGCGGTTCTTCAGGAGGTGTCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAAGCTgg                                                                                                                                              >  1:1131748/1‑150 (MQ=255)
         ccATCTGGTAGAAGTTATCATTCAGACATCAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGAGCTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTgg                                                                                                                                              <  2:1348096/149‑1 (MQ=255)
            tCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCTCCGGGCTGGCAt                                                                                                                                           <  1:1909165/150‑1 (MQ=255)
            tCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCTCCGGGCTGGCAt                                                                                                                                           <  1:1545635/150‑1 (MQ=255)
            tCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCAt                                                                                                                                           >  2:1728507/1‑150 (MQ=255)
            tCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCAAGCCCGGAGGCTGGCAt                                                                                                                                           >  1:1079677/1‑150 (MQ=255)
              tGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCAttt                                                                                                                                         <  2:54637/150‑1 (MQ=255)
               ggTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCAtttt                                                                                                                                        <  2:1022554/150‑1 (MQ=255)
               ggTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCAtttt                                                                                                                                        >  1:1007493/1‑150 (MQ=255)
                gTAGAAGTTATCATTCAGACAACAGCGGTTGCGCTGGAGGCTGAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCTCCGGGCTGGCAttttt                                                                                                                                       <  2:732530/150‑1 (MQ=255)
                gTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCAttttt                                                                                                                                       <  2:509689/150‑1 (MQ=255)
                  aGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCTCCGGGCTGGCAtttttta                                                                                                                                     <  1:571312/150‑3 (MQ=255)
                  aGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTaa                                                                                                                                     >  1:455872/1‑150 (MQ=255)
                   gAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCGGCCCGGAGGCTGGCATTTTTaaa                                                                                                                                    >  1:512251/1‑150 (MQ=255)
                      gTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCa                                                                                                                                 >  2:76839/1‑150 (MQ=255)
                      gTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCa                                                                                                                                 >  1:881831/1‑150 (MQ=255)
                       ttATCATTCAGACAACAGCGGTTCCGCTGGAGGCGCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAg                                                                                                                                <  1:1164235/150‑1 (MQ=255)
                        tATCATTCAGACAACACCGGTTCCGCTGGAGGCTCAGATCGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGa                                                                                                                               <  1:460059/150‑1 (MQ=255)
                         ttCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGAt                                                                                                                              <  2:1378462/149‑1 (MQ=255)
                         aTCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGAt                                                                                                                              <  1:1871384/150‑1 (MQ=255)
                           cATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCTGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATaa                                                                                                                            >  2:922538/1‑150 (MQ=255)
                            aTTCAGACAACAGCGGTTACGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATGCGGCCGCGGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGTCATGTTTAAATAAGATaaa                                                                                                                           >  2:111967/1‑150 (MQ=255)
                              tCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCAGGAGGTGGCGGCGCAACCGGCCGCTGGTAACTCAACCATAAAAAATTCCAACCCCGAGGCTGGAAATTTTAAATCAGATAAagt                                                                                                                         >  2:1076005/1‑150 (MQ=255)
                               cAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAagtc                                                                                                                        <  2:707540/150‑1 (MQ=255)
                                 gcctccaGCGGAACCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAagtcag                                                                                                                      >  2:2109304/6‑150 (MQ=255)
                                  acaacaGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAagtcagt                                                                                                                     >  1:1315469/1‑150 (MQ=255)
                                  acaacaGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAagtcagt                                                                                                                     >  2:735766/1‑150 (MQ=255)
                                    aacaGCGGTTCCGCTGGAGGCTCAGATAGAGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGAAGATGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCt                                                                                                                   <  1:862389/150‑1 (MQ=255)
                                     acaGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCACCCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCtt                                                                                                                  >  2:2075530/1‑150 (MQ=255)
                                     acaGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCAGCCGGCCGCTGGTAACTCAACCATAAAAAATGCCCGCCCGGAGGCTGGAATTTTTAAATAAGATAAAGTCAGTCtt                                                                                                                  >  1:399152/1‑150 (MQ=255)
                                       aGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTAt                                                                                                                >  2:1788278/1‑150 (MQ=255)
                                        gCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATg                                                                                                               >  2:1508333/1‑150 (MQ=255)
                                            ttCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTg                                                                                                           <  1:1260807/150‑1 (MQ=255)
                                              ccGCTGGAGGCTCAGATCGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGc                                                                                                         <  1:228941/150‑1 (MQ=255)
                                                  gggAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCttt                                                                                                     <  2:1881418/149‑1 (MQ=255)
                                                   ggAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTg                                                                                                    <  2:1927932/150‑1 (MQ=255)
                                                      ggCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGAtc                                                                                                 <  2:1409094/150‑1 (MQ=255)
                                                        cTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCCTCCGGCCGCTCGTAACTCAACCATAAAAAATTCCAGCCGGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATCCCTGGCCGTTGAtctc                                                                                               >  1:1337357/1‑150 (MQ=255)
                                                        cTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGAtctc                                                                                               <  2:690639/150‑1 (MQ=255)
                                                          cAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCtt                                                                                             <  1:649842/150‑1 (MQ=255)
                                                            gATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTa                                                                                           >  1:1687520/1‑150 (MQ=255)
                                                             aTAGCGGCGGATTTAGCGGGGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTAc                                                                                          <  2:851113/150‑1 (MQ=255)
                                                              tAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACg                                                                                         <  1:749470/150‑1 (MQ=255)
                                                                gcggcgGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGAc                                                                                       <  1:61932/150‑1 (MQ=255)
                                                                      gATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGt                                                                                 <  2:2062067/150‑1 (MQ=255)
                                                                      gATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGt                                                                                 <  2:652553/150‑1 (MQ=255)
                                                                       aTTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTa                                                                                >  1:1852821/1‑150 (MQ=255)
                                                                          tAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACgg                                                                             <  1:836540/150‑1 (MQ=255)
                                                                            gcggcgGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTg                                                                           >  1:1643017/1‑150 (MQ=255)
                                                                            gcggcgGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTg                                                                           >  2:1344663/1‑150 (MQ=255)
                                                                            gcggcgGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGACTGGCGTTTGATCTCTTTACGACCGTTGTACGGTg                                                                           >  2:1046911/1‑150 (MQ=255)
                                                                             cggcggGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGtac                                                                          <  1:2150126/150‑3 (MQ=255)
                                                                             cggcggGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGc                                                                          <  1:1823204/150‑1 (MQ=255)
                                                                             cggcggGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGc                                                                          <  1:1998414/150‑1 (MQ=255)
                                                                                    ggCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCg                                                                   <  2:1981745/150‑1 (MQ=255)
                                                                                          tcttctGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCATGGCCTTTGATCTCTTTACGAGCGTTGTACGGTGCTTATTCGCCCaga                                                             >  1:197880/1‑150 (MQ=255)
                                                                                           cttctGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCagag                                                            <  1:1398/150‑1 (MQ=255)
                                                                                             tctGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGct                                                          <  2:982405/150‑1 (MQ=255)
                                                                                             tctGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGACCAGAGct                                                          >  2:1425227/1‑150 (MQ=255)
                                                                                              ctGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGctt                                                         <  2:166134/150‑1 (MQ=255)
                                                                                                ggAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTGTTTACGCCCGTTGTACGGGGCTTTTTCGCCCAGAGcttcg                                                       >  1:808361/1‑149 (MQ=255)
                                                                                                   ggTGGCGGCGCATCCGGCCGCTGGTAACTCATCCATAAAAAATGCCTGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCg                                                    <  1:2056832/150‑1 (MQ=255)
                                                                                                   ggTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTTAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCg                                                    >  1:873247/1‑150 (MQ=255)
                                                                                                   ggTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCg                                                    <  1:152496/150‑1 (MQ=255)
                                                                                                     tGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGAt                                                  <  1:1574544/150‑1 (MQ=255)
                                                                                                      ggcggcGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTGTTATGCCTGGCCTTTGATCTCTTTACGACCGTGGTACGGTGCTTTTTCGCCCAGCGCTTCGTCGATa                                                 >  2:1065539/1‑150 (MQ=255)
                                                                                                      ggcggcGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATa                                                 >  1:973929/1‑150 (MQ=255)
                                                                                                      ggcggcGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATa                                                 <  1:171451/150‑1 (MQ=255)
                                                                                                      ggcggcGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATa                                                 <  1:2050345/150‑1 (MQ=255)
                                                                                                       gcggcgCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATAc                                                <  1:579222/150‑1 (MQ=255)
                                                                                                       gcggcgCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATAc                                                <  1:2109304/150‑1 (MQ=255)
                                                                                                        cggcgCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACg                                               <  1:986328/150‑1 (MQ=255)
                                                                                                        cggcgCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACg                                               <  1:494668/150‑1 (MQ=255)
                                                                                                        cggcgCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTGGTACGGTGATTTTTCGCCCAGAGCTTCTTCGATACg                                               >  1:1389731/1‑150 (MQ=255)
                                                                                                        cggcgCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCGTTGATCTCGTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACg                                               >  2:1287027/1‑150 (MQ=255)
                                                                                                           cgcATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAAt                                            >  1:2193002/1‑150 (MQ=255)
                                                                                                           cgcATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAAt                                            >  2:2132387/1‑150 (MQ=255)
                                                                                                                  ggCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTgg                                     <  1:1115580/150‑1 (MQ=255)
                                                                                                                       gtGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTa                                <  2:1863478/149‑1 (MQ=255)
                                                                                                                         ggTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGGATTTTTATATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCTCCCAGAGCTTCTTCGATACGATTCAGCTGGTTGTagt                              >  1:321440/1‑148 (MQ=255)
                                                                                                                         ggTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTAtt                              <  2:1768847/150‑1 (MQ=255)
                                                                                                                            aaCTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAg                           <  1:76839/150‑1 (MQ=255)
                                                                                                                             aCTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGc                          <  1:1344663/150‑1 (MQ=255)
                                                                                                                              cTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCa                         >  2:2176867/1‑150 (MQ=255)
                                                                                                                              cTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCa                         >  1:1495249/1‑150 (MQ=255)
                                                                                                                                cAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAac                       >  1:194095/1‑150 (MQ=255)
                                                                                                                                cAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAac                       >  1:1770115/1‑150 (MQ=255)
                                                                                                                                     tttaaaaaTGCCAGCCTCCGGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGt                  >  2:1758146/4‑150 (MQ=255)
                                                                                                                                      tAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGACCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTc                 >  2:351635/1‑150 (MQ=255)
                                                                                                                                       aaaaaaTGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCa                <  2:600006/150‑1 (MQ=255)
                                                                                                                                            aTGCCAGCCTCCGGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACg           >  1:1089893/1‑150 (MQ=255)
                                                                                                                                            aTGCCAGCCTCCGGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGAAACACGGTCatcacg           >  1:149857/1‑145 (MQ=255)
                                                                                                                                              gCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGc         >  2:731272/1‑150 (MQ=255)
                                                                                                                                                cAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTc       <  2:1315469/150‑1 (MQ=255)
                                                                                                                                                  gCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCAt     >  2:1565392/1‑150 (MQ=255)
                                                                                                                                                   cccGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATa    >  2:1665883/1‑150 (MQ=255)
                                                                                                                                                    ccGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAg   <  2:1813023/150‑1 (MQ=255)
                                                                                                                                                     cGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGa  <  2:453744/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CAGAAAAGAGCATCTGGTAGAAGTTATCATTCAGACAACAGCGGTTCCGCTGGAGGCTCAGATAGCGGCGGATTTAGCGGCGGGGGCGGTTCTTCTGGAGGTGGCGGCGCATCCGGCCGCTGGTAACTCAACCATAAAAAATGCCAGCCCGGAGGCTGGCATTTTTAAATCAGATAAAGTCAGTCTTATGCCTGGCCTTTGATCTCTTTACGACCGTTGTACGGTGCTTTTTCGCCCAGAGCTTCTTCGATACGAATCAGCTGGTTGTATTTAGCAACACGGTCAGAACGGCTCATAGA  >  NC_000913/2906458‑2906756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: