Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,926,039 A→T 5.4% Q231L (CAG→CTG)  queF → 7‑cyano‑7‑deazaguanine reductase (NADPH‑dependent)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,926,0390AT 5.4% 435.3 / 8.8 141Q231L (CAG→CTG) queF7‑cyano‑7‑deazaguanine reductase (NADPH‑dependent)
Reads supporting (aligned to +/- strand):  ref base A (75/58);  new base T (3/5);  total (78/63)
Fisher's exact test for biased strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.96e-01

Only 100 of 141 total aligned reads displayed.

TCAGCCACCTGCTGAAATCAAACTGCCTGATCACCCATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGT  >  NC_000913/2925892‑2926186
                                                                                                                                                   |                                                                                                                                                   
tCAGCCACCTGCTTAAATCAAACTGCCTGATCACCCATCAACCAGATTGGGGTTCGCTCCTAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGt                                                                                                                                                   <  1:1824860/150‑1 (MQ=255)
 cAGCCACCTGCTGAAATCAAACTGCCTGATCACCCATCAACCAGATTGGGGTTCGCTCCAAATTCAGAATCGTGGACGCCAACTTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTg                                                                                                                                                  >  1:1357400/1‑150 (MQ=255)
   gCCACGTGCTGAAATCAAACTGCCTGATCACCCATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACATGGTCTCAGTCCGTCATCACCACGAGTTCCACGCACAGTgag                                                                                                                                                >  2:350937/1‑148 (MQ=255)
   gCCACCTGCTGAAATCAAACTGCCTGATCACCCATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCg                                                                                                                                                <  2:1875748/150‑1 (MQ=255)
    ccACCTGCTGAAATCAAACTGCCTGATCACCCATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGCACTGTTCGt                                                                                                                                               <  2:182346/150‑1 (MQ=255)
     cACCTGCTGAAATCAAACTGCCTGATCACCCATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTg                                                                                                                                              >  2:531001/1‑150 (MQ=255)
     cACCTGCTGAAATCAAACTGCCTGATCACCCATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTg                                                                                                                                              <  1:1601875/150‑1 (MQ=255)
       ccTGCTGAAATCAAACTGCCTGATCACCCATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGa                                                                                                                                            >  1:1873131/1‑150 (MQ=255)
       ccTGCGGAAATCAACCTGCCTGATCACCCATCCACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTAACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGa                                                                                                                                            <  2:107740/150‑1 (MQ=255)
         tgctgAAATCAAACTGCCTGATCACCCATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAAc                                                                                                                                          <  1:1526514/150‑1 (MQ=255)
            tgAAATCAAACTGCCTGATCACCCATCACCCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGCCGCCAAATTGACAGAGAAAGACTGCTCCGTTACCTGGTCTCATTCCGTCACCACAACGAGTTCCACGATCAGTGCGTGGAACGCa                                                                                                                                       >  1:404902/1‑150 (MQ=255)
            tgAAATCAAACTGCCTGATCACCCATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGCACTGTTCGTGGAActcg                                                                                                                                       <  2:2006695/150‑4 (MQ=255)
             gAAATCAAACTGCCTGATCACCCATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCAt                                                                                                                                      >  1:1585835/1‑150 (MQ=255)
             gAAATCAAACTGCCAGATCACCCATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGCACTGTTCGTGGAActcgt                                                                                                                                      <  1:1326656/150‑5 (MQ=255)
               aaTCAAACTGCCTGATCACCCATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGCACTGTTCGTGGAActcgttg                                                                                                                                    <  1:848692/150‑7 (MQ=255)
               aaTCAAACTGCCTGATCACCCATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGCGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCCCGAACAGTGAGTGGAACGCTTCt                                                                                                                                    >  1:300105/1‑150 (MQ=255)
               aaTCAAACTGCCCGATCACCCATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCCCAACGAGTTCCACGAACAGTGCGTGGAACGCATCt                                                                                                                                    >  1:1194380/1‑150 (MQ=255)
                     aCTGCCTGATCACCCATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATg                                                                                                                              <  1:1782062/150‑1 (MQ=255)
                     aCTGCCTGATCACCCATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATg                                                                                                                              <  1:2099321/150‑1 (MQ=255)
                        gCCTGATCACCCATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGAcc                                                                                                                           <  2:1670296/150‑1 (MQ=255)
                         ccTGATCACCCATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCt                                                                                                                          >  1:531734/1‑150 (MQ=255)
                         ccTGATCACCCATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCt                                                                                                                          >  1:1097488/1‑150 (MQ=255)
                           tGATCACCCATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGt                                                                                                                        >  2:277279/1‑150 (MQ=255)
                                aCCCATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGc                                                                                                                   <  1:894199/150‑1 (MQ=255)
                                aCCCATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGc                                                                                                                   >  2:1516144/1‑150 (MQ=255)
                                 cccATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGCAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCAAGTTTAATGCCCTGTTACGCt                                                                                                                  >  2:1976266/1‑150 (MQ=255)
                                 cccATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCt                                                                                                                  >  2:1976261/1‑150 (MQ=255)
                                 cccATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCt                                                                                                                  >  1:2022097/1‑150 (MQ=255)
                                 cacATCAAACAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGTAACGCATCTTTAATGACCTGTTACGCt                                                                                                                  <  1:1274771/148‑1 (MQ=255)
                                   cATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGATGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTc                                                                                                                <  1:1935659/150‑1 (MQ=255)
                                    aTCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCt                                                                                                               >  2:230685/1‑150 (MQ=255)
                                     tCAACCTGATTGGGGTTCGCTCCCAATTCAGTATCGTGGACTCCAAATTGACAGAGAAAAACTGGTGCGTTACCTGGTCTCATTGCGTCATCACAACGAGTTCCACGAACAGTGAGTGGAACGCATCTTTAATGACCTGTTACGCTTCTg                                                                                                              <  1:1019189/150‑1 (MQ=255)
                                      cAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTgc                                                                                                             >  2:219799/1‑150 (MQ=255)
                                      cAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTAAGCTTCTgc                                                                                                             >  2:215931/1‑150 (MQ=255)
                                       aaCCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTgcc                                                                                                            <  1:658690/150‑1 (MQ=255)
                                       aaCCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAAATGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTgcc                                                                                                            >  1:1211822/1‑150 (MQ=255)
                                        aCCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTgcca                                                                                                           >  1:89817/1‑150 (MQ=255)
                                         ccAGATTGGGGTTCCCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTACACGAACAGTGCGTGGAACGCATCTTTAATGACCTTATACGCTTCTgcaac                                                                                                          >  2:1700496/1‑147 (MQ=255)
                                           aGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGTCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTgccagcc                                                                                                        >  2:1814533/1‑150 (MQ=255)
                                            gATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGGCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTgccagcca                                                                                                       <  2:1553562/150‑1 (MQ=255)
                                             aTTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGccagccag                                                                                                      <  2:1182560/150‑1 (MQ=255)
                                                ggggTTGGGTCCAATTTCAGTATCGTTGACTCCAAATTGACAGAGATAATCAGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGaaa                                                                                                   <  1:113655/150‑1 (MQ=255)
                                                  ggTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGaaaaa                                                                                                 >  2:2033062/1‑150 (MQ=255)
                                                     tCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTg                                                                                              <  1:940269/150‑1 (MQ=255)
                                                     tCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCGGCGTTACCTGGGCTCATTCCGTCATCACACCGAGTTCCACGAACAGTGCGTGGAACGCATCTTTACTGACCTGTTACGCTTCTGCCAGCCAGAAAAATTg                                                                                              <  1:375275/150‑1 (MQ=255)
                                                        cTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCTGAAAAATTGAGc                                                                                           <  1:1286259/150‑1 (MQ=255)
                                                         tCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCg                                                                                          <  2:695775/150‑1 (MQ=255)
                                                           cAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGtt                                                                                        >  1:1791042/1‑150 (MQ=255)
                                                               ttCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACg                                                                                    <  1:397955/150‑1 (MQ=255)
                                                                     aTCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGGCTCATTCCGTCATCAAAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGtt                                                                              <  2:1293965/150‑1 (MQ=255)
                                                                      tCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTta                                                                             <  1:845446/150‑1 (MQ=255)
                                                                       cGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGGTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTtat                                                                            <  2:638648/150‑1 (MQ=255)
                                                                         tGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATAc                                                                          <  2:1789414/150‑1 (MQ=255)
                                                                          ggACGCCAAATTGACAGAGAAACACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGCCCTGTTACGCTTCTGCCAGCCAGAAAAAGTGAGCGTTTACGCACGTTATAcc                                                                         >  1:1054479/1‑150 (MQ=255)
                                                                             agacAAATTGACAGAGAAAAACTTCTTCGTTTCCTGGTCTCATTCCGTCATAACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCATCCAGAAAAATTGAGCGTTTACGCACGTTATACCcgt                                                                      <  2:1194380/147‑1 (MQ=255)
                                                                               ccAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGATCAGTGCGTGGAACGCATCTTTAATGAACTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACTCTCGTTATACCcgtcg                                                                    >  1:1350191/1‑150 (MQ=255)
                                                                                    ttGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCg                                                               <  2:1898376/150‑1 (MQ=255)
                                                                                     tGACAGAGAAAAACTGCTTCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCgg                                                              <  2:1336507/150‑1 (MQ=255)
                                                                                      gACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCggg                                                             >  1:1471826/1‑149 (MQ=255)
                                                                                      gACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGt                                                             >  1:1545029/1‑150 (MQ=255)
                                                                                       aCAGAGAAAAACTGCTGCGTTACCTGGTCTTATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTc                                                            <  1:2086315/150‑1 (MQ=255)
                                                                                        cAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCt                                                           >  2:2062640/1‑150 (MQ=255)
                                                                                         agagAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCTTCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTg                                                          <  2:2106991/150‑1 (MQ=255)
                                                                                          gagaAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCTTGGCGGTCTgg                                                         >  2:1295909/1‑150 (MQ=255)
                                                                                          gagaAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTgg                                                         <  2:531734/150‑1 (MQ=255)
                                                                                          gagaAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTgg                                                         >  1:1051516/1‑150 (MQ=255)
                                                                                            taaaaaCTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGAc                                                       <  2:2094598/149‑1 (MQ=255)
                                                                                            gaAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGAc                                                       <  2:1495248/150‑1 (MQ=255)
                                                                                            gaAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGAc                                                       >  1:1123973/1‑150 (MQ=255)
                                                                                            gaAAAACTGCTGCGTTACCTGGTCTCAATCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCAATAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGAACGTTATACCCGTCGTGGCGTTCTAGAc                                                       >  2:1646404/1‑150 (MQ=255)
                                                                                             aaaaaCTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACTAACAGTGCGTGGAACGCATCTTTAATGTCCTGTTACGCTTCTGCCAGCAAGAAAAATTTAGCGTTTACGGACTTTATAACCGTAGTTGCGGTCTGGACa                                                      >  2:160578/1‑150 (MQ=255)
                                                                                               aaaCTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACAtt                                                    <  1:1722526/150‑1 (MQ=255)
                                                                                                  ctgctgCGGTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAAc                                                 <  2:2045708/150‑1 (MQ=255)
                                                                                                      tgcGTTACCTGGTCTTATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTACCCCgg                                             >  2:1731439/1‑149 (MQ=255)
                                                                                                      tgcGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACATGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGt                                             >  1:616301/1‑150 (MQ=255)
                                                                                                       gcGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTg                                            >  1:276697/1‑150 (MQ=255)
                                                                                                       gcGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTg                                            >  1:2127233/1‑150 (MQ=255)
                                                                                                            aCCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCa                                       >  1:1748030/1‑150 (MQ=255)
                                                                                                              gtGGTCTCATTCCGTCTTCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACGTGTTACGGTTGTGCCAGCGAGAAAAATAGAGCGTTTGCGCACGTGTTACCCGTCGTGGCGGTCTGGACATTAACCGGTGGCGAAGt                                     >  1:143446/2‑150 (MQ=255)
                                                                                                                ggTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTaa                                   <  1:1850637/150‑1 (MQ=255)
                                                                                                                   ctcATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAg                                >  2:1097008/1‑150 (MQ=255)
                                                                                                                     cATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCCGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTTGCGCAGTAATAGCg                              >  2:81479/1‑150 (MQ=255)
                                                                                                                          cGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACTCACGTTATACCCGTCTTGGCGGTATGGACATTAACCCGTGGCGCAGTAATAGCGAtttt                         >  1:1964602/1‑150 (MQ=255)
                                                                                                                          cGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGAtttt                         <  1:1897892/150‑1 (MQ=255)
                                                                                                                           gTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTg                        >  2:763945/1‑150 (MQ=255)
                                                                                                                           gTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTg                        <  2:1873131/150‑1 (MQ=255)
                                                                                                                             catcaCAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCTGTAATAGCGATTTTGTc                      >  1:270120/1‑150 (MQ=255)
                                                                                                                             catcaCAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTc                      <  1:1516144/150‑1 (MQ=255)
                                                                                                                                 acaACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCAt                  >  2:1104492/1‑150 (MQ=255)
                                                                                                                                   gatgcgTTCCACGCACTGTTCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCg                >  2:1151616/6‑150 (MQ=255)
                                                                                                                                   aaCGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCg                <  1:692482/150‑1 (MQ=255)
                                                                                                                                      gcgTTCCACGCACTGTTCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAAAAGCGATTTTGTCCCATCGAcc             >  2:571973/3‑150 (MQ=255)
                                                                                                                                      gAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGAcc             >  2:1908327/1‑150 (MQ=255)
                                                                                                                                      gAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGAcc             <  1:453404/150‑1 (MQ=255)
                                                                                                                                       cgTTCCACGCACTGTTCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCCTCGTGGAGGTCTGGACATTAACACGTGGCGCAGTAATAGAGATATTGTCCCATCGACca            >  2:1372616/2‑150 (MQ=255)
                                                                                                                                       aGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACca            >  2:1941437/1‑150 (MQ=255)
                                                                                                                                        gTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGCCcac           >  1:202823/1‑150 (MQ=255)
                                                                                                                                            cACGAACAGTGCGTGGAACGCATCTTTAATGAACTGTTACGCTTCTGCCAGCCAGAAAAATAGAGCGTTAACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCCTCGACAACAAGa       >  1:321880/1‑150 (MQ=255)
                                                                                                                                               gAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTACCATCGACCACAATACTg    >  2:2007508/1‑150 (MQ=255)
                                                                                                                                                 aCAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGt  >  2:1103870/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TCAGCCACCTGCTGAAATCAAACTGCCTGATCACCCATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGT  >  NC_000913/2925892‑2926186

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑