Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,926,042 G→T 5.2% C232F (TGC→TTC)  queF → 7‑cyano‑7‑deazaguanine reductase (NADPH‑dependent)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,926,0420GT 5.2% 438.0 / 7.6 141C232F (TGC→TTC) queF7‑cyano‑7‑deazaguanine reductase (NADPH‑dependent)
Reads supporting (aligned to +/- strand):  ref base G (74/59);  new base T (3/5);  total (77/64)
Fisher's exact test for biased strand distribution p-value = 4.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 146 total aligned reads displayed.

CAGCCACCTGCTGAAATCAAACTGCCTGATCACCCATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGC  >  NC_000913/2925893‑2926191
                                                                                                                                                     |                                                                                                                                                     
cAGCCACCTGCTGAAATCAAACTGCCTGATCACCCATCAACCAGATTGGGGTTCGCTCCAAATTCAGAATCGTGGACGCCAACTTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTg                                                                                                                                                       >  1:1357400/1‑150 (MQ=255)
  gCCACGTGCTGAAATCAAACTGCCTGATCACCCATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACATGGTCTCAGTCCGTCATCACCACGAGTTCCACGCACAGTgag                                                                                                                                                     >  2:350937/1‑148 (MQ=255)
  gCCACCTGCTGAAATCAAACTGCCTGATCACCCATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCg                                                                                                                                                     <  2:1875748/150‑1 (MQ=255)
   ccACCTGCTGAAATCAAACTGCCTGATCACCCATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGCACTGTTCGt                                                                                                                                                    <  2:182346/150‑1 (MQ=255)
    cACCTGCTGAAATCAAACTGCCTGATCACCCATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTg                                                                                                                                                   <  1:1601875/150‑1 (MQ=255)
      ccTGCTGAAATCAAACTGCCTGATCACCCATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGa                                                                                                                                                 >  1:1873131/1‑150 (MQ=255)
      ccTGCGGAAATCAACCTGCCTGATCACCCATCCACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTAACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGa                                                                                                                                                 <  2:107740/150‑1 (MQ=255)
           tgAAATCAAACTGCCTGATCACCCATCACCCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGCCGCCAAATTGACAGAGAAAGACTGCTCCGTTACCTGGTCTCATTCCGTCACCACAACGAGTTCCACGATCAGTGCGTGGAACGCa                                                                                                                                            >  1:404902/1‑150 (MQ=255)
           tgAAATCAAACTGCCTGATCACCCATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGCACTGTTCGTGGAActcg                                                                                                                                            <  2:2006695/150‑4 (MQ=255)
            gAAATCAAACTGCCTGATCACCCATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCAt                                                                                                                                           >  2:1850637/1‑150 (MQ=255)
            gAAATCAAACTGCCAGATCACCCATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGCACTGTTCGTGGAActcgt                                                                                                                                           <  1:1326656/150‑5 (MQ=255)
              aaTCAAACTGCCTGATCACCCATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGCACTGTTCGTGGAActcgttg                                                                                                                                         <  1:848692/150‑7 (MQ=255)
              aaTCAAACTGCCTGATCACCCATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGCGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCCCGAACAGTGAGTGGAACGCTTCt                                                                                                                                         >  1:300105/1‑150 (MQ=255)
              aaTCAAACTGCCCGATCACCCATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCCCAACGAGTTCCACGAACAGTGCGTGGAACGCATCt                                                                                                                                         >  1:1194380/1‑150 (MQ=255)
                 cAAACTGCCTGATCACCCATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTa                                                                                                                                      >  2:1302970/1‑150 (MQ=255)
                    aCTGCCTGATCACCCATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATg                                                                                                                                   <  1:2099321/150‑1 (MQ=255)
                    aCTGCCTGATCACCCATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATg                                                                                                                                   <  1:1782062/150‑1 (MQ=255)
                        ccTGATCACCCATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCt                                                                                                                               >  1:1097488/1‑150 (MQ=255)
                        ccTGATCACCCATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCt                                                                                                                               >  1:531734/1‑150 (MQ=255)
                         cTGATCACCCATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTg                                                                                                                              >  1:2168633/1‑150 (MQ=255)
                               aCCCATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGc                                                                                                                        >  2:1516144/1‑150 (MQ=255)
                               aCCCATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGc                                                                                                                        <  1:894199/150‑1 (MQ=255)
                                cccATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGCAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCAAGTTTAATGCCCTGTTACGCt                                                                                                                       >  2:1976266/1‑150 (MQ=255)
                                cccATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCt                                                                                                                       >  1:2022097/1‑150 (MQ=255)
                                cccATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGACAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCt                                                                                                                       <  2:238851/150‑1 (MQ=255)
                                cacATCAAACAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGTAACGCATCTTTAATGACCTGTTACGCt                                                                                                                       <  1:1274771/148‑1 (MQ=255)
                                  cATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGATGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTc                                                                                                                     <  1:1935659/150‑1 (MQ=255)
                                   aTCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCt                                                                                                                    >  2:230685/1‑150 (MQ=255)
                                    tCAACCTGATTGGGGTTCGCTCCCAATTCAGTATCGTGGACTCCAAATTGACAGAGAAAAACTGGTGCGTTACCTGGTCTCATTGCGTCATCACAACGAGTTCCACGAACAGTGAGTGGAACGCATCTTTAATGACCTGTTACGCTTCTg                                                                                                                   <  1:1019189/150‑1 (MQ=255)
                                    tCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTg                                                                                                                   <  2:498754/150‑1 (MQ=255)
                                     cAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTgc                                                                                                                  >  2:2088763/1‑150 (MQ=255)
                                     cAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTAAGCTTCTgc                                                                                                                  >  2:215931/1‑150 (MQ=255)
                                      aaCCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTgcc                                                                                                                 <  1:658690/150‑1 (MQ=255)
                                       aCCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTgcca                                                                                                                >  1:89817/1‑150 (MQ=255)
                                        ccAGATTGGGGTTCCCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTACACGAACAGTGCGTGGAACGCATCTTTAATGACCTTATACGCTTCTgcaac                                                                                                               >  2:1700496/1‑147 (MQ=255)
                                           gATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGGCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTgccagcca                                                                                                            <  2:1553562/150‑1 (MQ=255)
                                              tGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGaa                                                                                                         >  1:1568395/1‑150 (MQ=255)
                                               ggggTTGGGTCCAATTTCAGTATCGTTGACTCCAAATTGACAGAGATAATCAGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGaaa                                                                                                        <  1:113655/150‑1 (MQ=255)
                                                 ggTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGaaaaa                                                                                                      >  2:2033062/1‑150 (MQ=255)
                                                    tCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTg                                                                                                   <  1:940269/150‑1 (MQ=255)
                                                    tCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCGGCGTTACCTGGGCTCATTCCGTCATCACACCGAGTTCCACGAACAGTGCGTGGAACGCATCTTTACTGACCTGTTACGCTTCTGCCAGCCAGAAAAATTg                                                                                                   <  1:375275/150‑1 (MQ=255)
                                                        tCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCg                                                                                               <  2:695775/150‑1 (MQ=255)
                                                         ccAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGt                                                                                              >  2:1153890/1‑150 (MQ=255)
                                                          cAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGtt                                                                                             >  1:1791042/1‑150 (MQ=255)
                                                              ttCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACg                                                                                         <  1:1302970/150‑1 (MQ=255)
                                                                     tCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTta                                                                                  <  1:845446/150‑1 (MQ=255)
                                                                      cGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGGTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTtat                                                                                 <  2:638648/150‑1 (MQ=255)
                                                                        tGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATAc                                                                               <  2:1789414/150‑1 (MQ=255)
                                                                         ggACGCCAAATTGACAGAGAAACACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGCCCTGTTACGCTTCTGCCAGCCAGAAAAAGTGAGCGTTTACGCACGTTATAcc                                                                              >  1:1054479/1‑150 (MQ=255)
                                                                         ggACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATAcc                                                                              >  2:1236185/1‑150 (MQ=255)
                                                                           acaccAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCcg                                                                            >  1:1886283/4‑150 (MQ=255)
                                                                            agacAAATTGACAGAGAAAAACTTCTTCGTTTCCTGGTCTCATTCCGTCATAACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCATCCAGAAAAATTGAGCGTTTACGCACGTTATACCcgt                                                                           <  2:1194380/147‑1 (MQ=255)
                                                                              ccAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCcgtcg                                                                         >  1:990170/1‑150 (MQ=255)
                                                                                 aaTTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTgg                                                                      <  1:448935/150‑1 (MQ=255)
                                                                                   ttGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCg                                                                    <  2:1898376/150‑1 (MQ=255)
                                                                                     gACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGt                                                                  >  1:1545029/1‑150 (MQ=255)
                                                                                      aCAGAGAAAAACTGCTGCGTTACCTGGTCTTATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTc                                                                 <  1:2086315/150‑1 (MQ=255)
                                                                                         gagaAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGCGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTgg                                                              >  1:1476402/1‑150 (MQ=255)
                                                                                         gagaAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCTTGGCGGTCTgg                                                              >  2:1295909/1‑150 (MQ=255)
                                                                                         gagaAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTgg                                                              <  2:531734/150‑1 (MQ=255)
                                                                                         gagaAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTgg                                                              >  1:1051516/1‑150 (MQ=255)
                                                                                         gagaAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAAATGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTgg                                                              <  2:1097488/150‑1 (MQ=255)
                                                                                           taaaaaCTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGAc                                                            <  2:2094598/149‑1 (MQ=255)
                                                                                           gaAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGAc                                                            <  2:1495248/150‑1 (MQ=255)
                                                                                           gaAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGAc                                                            >  1:1123973/1‑150 (MQ=255)
                                                                                           gaAAAACTGCTGCGTTACCTGGTCTCAATCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCAATAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGAACGTTATACCCGTCGTGGCGTTCTAGAc                                                            >  2:1646404/1‑150 (MQ=255)
                                                                                            aaaaaCTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACTAACAGTGCGTGGAACGCATCTTTAATGTCCTGTTACGCTTCTGCCAGCAAGAAAAATTTAGCGTTTACGGACTTTATAACCGTAGTTGCGGTCTGGACa                                                           >  2:160578/1‑150 (MQ=255)
                                                                                              aaaCTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACAtt                                                         <  1:1722526/150‑1 (MQ=255)
                                                                                                 ctgctgCGGTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAAc                                                      <  2:2045708/150‑1 (MQ=255)
                                                                                                       cGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTgg                                                <  1:277279/150‑1 (MQ=255)
                                                                                                           aCCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCa                                            >  1:1748030/1‑150 (MQ=255)
                                                                                                             gtGGTCTCATTCCGTCTTCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACGTGTTACGGTTGTGCCAGCGAGAAAAATAGAGCGTTTGCGCACGTGTTACCCGTCGTGGCGGTCTGGACATTAACCGGTGGCGAAGt                                          >  1:143446/2‑150 (MQ=255)
                                                                                                             cTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGACCGCATCTTTAATGCCCTGTTACGCTTCTGACCGCCAGAAAAATTGAGCGTGTACGCCCGTTATACCCGGCGTGGGGGGCGGGCCAGTAACCCGTGGCGCAgg                                          >  1:143442/1‑149 (MQ=255)
                                                                                                               ggTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTaa                                        <  1:1850637/150‑1 (MQ=255)
                                                                                                                    cATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCCGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTTGCGCAGTAATAGCg                                   >  2:81479/1‑150 (MQ=255)
                                                                                                                    cATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCTTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCg                                   >  1:539637/1‑150 (MQ=255)
                                                                                                                     aTTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGACCGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGGAATAGCGa                                  >  1:388177/1‑150 (MQ=255)
                                                                                                                       tCCGTAATCACAACGAGTTACACGAACAGTTCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGAtt                                <  2:1979671/150‑1 (MQ=255)
                                                                                                                         cGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACTCACGTTATACCCGTCTTGGCGGTATGGACATTAACCCGTGGCGCAGTAATAGCGAtttt                              >  1:1964602/1‑150 (MQ=255)
                                                                                                                         cGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGAtttt                              >  2:1684007/1‑150 (MQ=255)
                                                                                                                         cGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGAtttt                              >  2:1760555/1‑150 (MQ=255)
                                                                                                                         cGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGAtttt                              >  2:2112073/1‑150 (MQ=255)
                                                                                                                          gTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTg                             >  2:763945/1‑150 (MQ=255)
                                                                                                                            catcaCAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCTGTAATAGCGATTTTGTc                           >  1:270120/1‑150 (MQ=255)
                                                                                                                            catcaCAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTc                           <  1:1516144/150‑1 (MQ=255)
                                                                                                                                 caccGAGTTCCACGAACAGTGCGAGGAACGCATCTATTATGACCTCTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTATACGCACGTTATACCCGTCGAGGAGGTGTGGACATTAACCCATGGCGAAGTAATAGCGATTTTGTCCCATc                      >  2:1961096/4‑150 (MQ=255)
                                                                                                                                  gatgcgTTCCACGCACTGTTCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCg                     >  2:1151616/6‑150 (MQ=255)
                                                                                                                                  aaCGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCg                     <  1:692482/150‑1 (MQ=255)
                                                                                                                                     gcgTTCCACGCACTGTTCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAAAAGCGATTTTGTCCCATCGAcc                  >  2:571973/3‑150 (MQ=255)
                                                                                                                                     gAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGAcc                  <  1:1221858/150‑1 (MQ=255)
                                                                                                                                      cgTTCCACGCACTGTTCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCCTCGTGGAGGTCTGGACATTAACACGTGGCGCAGTAATAGAGATATTGTCCCATCGACca                 >  2:1372616/2‑150 (MQ=255)
                                                                                                                                      aGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACca                 >  2:1941437/1‑150 (MQ=255)
                                                                                                                                       gTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGCCcac                >  1:202823/1‑150 (MQ=255)
                                                                                                                                           cACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGa            >  2:1886428/1‑150 (MQ=255)
                                                                                                                                           cACGAACAGTGCGTGGAACGCATCTTTAATGAACTGTTACGCTTCTGCCAGCCAGAAAAATAGAGCGTTAACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCCTCGACAACAAGa            >  1:321880/1‑150 (MQ=255)
                                                                                                                                              gAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTACCATCGACCACAATACTg         >  2:2007508/1‑150 (MQ=255)
                                                                                                                                                aCAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGt       >  2:1103870/1‑150 (MQ=255)
                                                                                                                                                   gTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGCGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTGCg    <  2:1434473/150‑1 (MQ=255)
                                                                                                                                                     gCGTGGAACGCTTCTTTAATGACCTGTTACGATTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGGGGTCTCGACATTAACCCGTGGCGCAGTAATAGCGTTTTTGTCCCATCGACCACAAGACTGGTTCGGc  <  1:1646404/150‑1 (MQ=255)
                                                                                                                                                     gCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGc  >  2:2108243/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CAGCCACCTGCTGAAATCAAACTGCCTGATCACCCATCAACCAGATTGGGGTTCGCTCCAAATTCAGTATCGTGGACGCCAAATTGACAGAGAAAAACTGCTGCGTTACCTGGTCTCATTCCGTCATCACAACGAGTTCCACGAACAGTGCGTGGAACGCATCTTTAATGACCTGTTACGCTTCTGCCAGCCAGAAAAATTGAGCGTTTACGCACGTTATACCCGTCGTGGCGGTCTGGACATTAACCCGTGGCGCAGTAATAGCGATTTTGTCCCATCGACCACAAGACTGGTTCGGC  >  NC_000913/2925893‑2926191

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑