Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,045,124 T→C 7.6% intergenic (+23/+34) bglA → / ← ygfF 6‑phospho‑beta‑glucosidase A/putative NAD(P)‑dependent oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,045,1240TC 7.6% 346.3 / 14.4 118intergenic (+23/+34)bglA/ygfF6‑phospho‑beta‑glucosidase A/putative NAD(P)‑dependent oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (60/49);  new base C (5/4);  total (65/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.43e-01

Only 100 of 121 total aligned reads displayed.

CAGCAAACGCTACGGCTTTATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGCTGCATGGGGA  >  NC_000913/3044975‑3045273
                                                                                                                                                     |                                                                                                                                                     
cAGCAAACGCTACGGCTTTATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAAt                                                                                                                                                       >  2:56353/1‑150 (MQ=255)
 aGCAAACGCTACGGCTTTATCTATGTGAATAAACATGACGACGGTCCTGGCGATAAGACGCGTTAACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGAGAGAAGCTATAAGTCGATGAAGTAACGGATGCAATa                                                                                                                                                      >  1:1151723/1‑150 (MQ=255)
  gCAAACGCTACGGCTTTATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGTTGATTGCCAGCAACGGCGAGAAGCTTTAAGTAGATGAAGTACCGGATGCAATAc                                                                                                                                                     >  1:2208566/1‑150 (MQ=255)
   cAAACGCTACGGCTTTATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACt                                                                                                                                                    <  2:693440/150‑1 (MQ=255)
    aaaCGCTACGGCTTTATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACttg                                                                                                                                                  >  2:2053703/1‑150 (MQ=255)
       cGCTACGGCTTTATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACttgtt                                                                                                                                                >  1:1029406/1‑150 (MQ=255)
        gCTACGGCTTTATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACttgttg                                                                                                                                               <  1:1614613/150‑1 (MQ=255)
        gCTACGGCTTTATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACttgttg                                                                                                                                               <  1:1443790/150‑1 (MQ=255)
           aCGGCTTTATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCAt                                                                                                                                            >  1:64182/1‑150 (MQ=255)
            cGGCTTTATCTATGTGAATAAACATGACGACCGTACTGTCGATATGTCGCGTTAACGTAAGAATAGCTTTATCTGGTACAAATAGGTGATTGACAGCAACGGCGAGAAGATTTAAGTCGATGAAATACCGGATTCAATACTAGTTGCAta                                                                                                                                           >  1:1075977/1‑149 (MQ=255)
             ggCTTTATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAACAAGTATTGCATcc                                                                                                                                          <  2:1771308/150‑1 (MQ=255)
               cTTTATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAACAAGTATTGCATCCgg                                                                                                                                        <  1:27084/150‑1 (MQ=255)
                tttATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAACAAGTATTGCATCCGGt                                                                                                                                       <  2:1145788/150‑1 (MQ=255)
                  tATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCa                                                                                                                                     <  1:2127200/150‑1 (MQ=255)
                  tATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCa                                                                                                                                     <  2:4515/150‑1 (MQ=255)
                    tCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGa                                                                                                                                   >  1:150383/1‑150 (MQ=255)
                      tATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACa                                                                                                                                 >  1:1335516/1‑150 (MQ=255)
                      tATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTGTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACa                                                                                                                                 >  2:2071161/1‑150 (MQ=255)
                        tgtgAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAAc                                                                                                                               <  1:566311/150‑1 (MQ=255)
                            aaTAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTcc                                                                                                                           <  2:2213361/150‑1 (MQ=255)
                             aTAAACATCACGACGTTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTccc                                                                                                                          <  1:230142/150‑1 (MQ=255)
                                  cATGACGACGGTACTGGCGATATGTCGCGTTCACGAAAGAAGAGCTTTAACTGGTACAAAGAGGCGATAGCCAGCAACGGCGAGAAGCTTTAAGTCGTTGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGttt                                                                                                                     <  1:1809200/150‑1 (MQ=255)
                                      acgacgGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTAtt                                                                                                                 <  2:1537369/150‑1 (MQ=255)
                                         acgGTACTGGCGATATGTCGCGTTCAGGTAAGAATAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGTAGCTTTAAGTCGATGAAGTACCGGCTGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTcc                                                                                                              <  1:1462438/150‑1 (MQ=255)
                                         acgGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTcc                                                                                                              <  2:1029406/150‑1 (MQ=255)
                                         acgGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTcc                                                                                                              >  2:1232572/1‑150 (MQ=255)
                                         acgGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTcc                                                                                                              <  2:1881771/150‑1 (MQ=255)
                                          cgGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTccc                                                                                                             >  1:932873/1‑150 (MQ=255)
                                               cTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTcccgcccg                                                                                                        <  2:1273022/150‑1 (MQ=255)
                                                 ggCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGATGCGTTAAGTCGATGAAGTACCGGAAGCAATAGTTGTTGCATCCCGTCAGACAACTTCCCTGTTGTATTTCCcgcccgcc                                                                                                      >  2:425443/1‑150 (MQ=255)
                                                 ggCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCcgcccgcc                                                                                                      >  1:1919095/1‑150 (MQ=255)
                                                 ggCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCcgcccgcc                                                                                                      >  1:2102100/1‑150 (MQ=255)
                                                 ggCGATATGTCGCGTGCACGTAAGAAGCGCGGTAACTGGTACAAAGAGGTGATTGCCAGCACCGGCGAGAAGCTTTATGTCGATGAAGTACCGGATGCAATACGGGTTGCATCCGGTCAGACAACTTCGCTGTGTTATTTCCcgcccgcc                                                                                                      >  2:767076/1‑150 (MQ=255)
                                                      tatGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATc                                                                                                 >  2:2164632/1‑150 (MQ=255)
                                                      tatGTCGCGGGCACGTAAGATGAGCTTTAACTGGTACAAAGAGGTGAGTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATc                                                                                                 <  2:377145/150‑1 (MQ=255)
                                                         gTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGAt                                                                                              >  1:636047/1‑150 (MQ=255)
                                                         gTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGAt                                                                                              >  2:1918488/1‑150 (MQ=255)
                                                          tCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATa                                                                                             <  2:64182/150‑1 (MQ=255)
                                                              gTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAAc                                                                                         <  1:1611718/150‑1 (MQ=255)
                                                                 cACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTc                                                                                      >  1:1276646/1‑150 (MQ=255)
                                                                 cACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTc                                                                                      >  2:803290/1‑150 (MQ=255)
                                                                    gTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCg                                                                                   >  2:2142199/1‑150 (MQ=255)
                                                                     tAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGAAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGt                                                                                  <  1:388582/150‑1 (MQ=255)
                                                                         aagaGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACg                                                                              <  1:1982655/150‑1 (MQ=255)
                                                                                ttAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAgg                                                                       >  1:1090748/1‑150 (MQ=255)
                                                                                 tAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGc                                                                      >  2:166360/1‑150 (MQ=255)
                                                                                 tAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGc                                                                      <  2:135925/150‑1 (MQ=255)
                                                                                 tAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGc                                                                      <  1:1866580/150‑1 (MQ=255)
                                                                                   aCTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCCTCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCGCGTGACGGAAGAGGCtt                                                                    >  2:230526/1‑150 (MQ=255)
                                                                                   aCTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCtt                                                                    >  1:531671/1‑150 (MQ=255)
                                                                                   aCTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCAGATGCAATACTTGTTGCATCCGTTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCtt                                                                    <  1:370203/150‑1 (MQ=255)
                                                                                      ggTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGAAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTAt                                                                 <  1:1330717/150‑1 (MQ=255)
                                                                                       gTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATc                                                                <  1:1681729/150‑1 (MQ=255)
                                                                                         aCAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGTTTTATCAc                                                              <  1:1467625/150‑1 (MQ=255)
                                                                                          cAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACt                                                             >  1:601541/1‑150 (MQ=255)
                                                                                          cAAAGACGTGATTGCCAGCAACGGCGAGAAGCTATAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCATGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACt                                                             >  1:1514479/1‑150 (MQ=255)
                                                                                           aaaGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACtt                                                            <  2:907902/150‑1 (MQ=255)
                                                                                            aaGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTta                                                           <  1:16837/150‑1 (MQ=255)
                                                                                              gagGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACACCTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTtagt                                                         >  1:177057/1‑150 (MQ=255)
                                                                                              gagGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGAAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCACGTGACGTAAGAGGCTTTATCACTtagt                                                         >  1:1832598/1‑150 (MQ=255)
                                                                                                 gTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGc                                                      >  1:979413/1‑150 (MQ=255)
                                                                                                  tGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGcc                                                     <  1:1016697/150‑1 (MQ=255)
                                                                                                  ggATTGCCAGCAACGGCGAGAAGCTTTAAGGCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGcc                                                     <  1:1773686/149‑1 (MQ=255)
                                                                                                       gCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACATCTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGAGGTAAGAGGCTTTATCACTTAGTAGCCAGACa                                                >  2:1337789/1‑150 (MQ=255)
                                                                                                        ccAGCAACGGCGAGAAGCTTTAAGTCTATGAAGTACCGCATGCAATACTTGATGCATCCGGTCAGACAACGACCCTGTTTTATTTCCCGCCCGCCAACTCGATAAAAATTCCAGTCACGTAAGAGGCTTAATCACTTAGTAGCCAGACaa                                               >  1:345273/1‑150 (MQ=255)
                                                                                                           gCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCTGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAACCTTCCCGTGACGTATGAGGCTTTATCACTTAGTAGCCAGACAATgg                                            <  2:94959/150‑1 (MQ=255)
                                                                                                              aCGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCt                                         <  1:850269/150‑1 (MQ=255)
                                                                                                                   gagaAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGcgcg                                    <  2:932873/150‑1 (MQ=255)
                                                                                                                   gagaAGCTTTAAGTCGATGAAGTAACGGATGCAATACTTGTTGCATCCGGGCAGACAACTTCCATGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGcgcg                                    <  2:193865/150‑1 (MQ=255)
                                                                                                                    agaAGCTTTAAGTCGATGAAGTACCGGATGCAATACTGGTTGCCTCCGGTCAGACAACTTCCATGTTTTATTTCCCGCCCGCCAAATCGATTAACCTTCCCGGGACGTAAGAGGCTTTATCACTTAGTAGCCCGACAATGTCATGCGCGa                                   >  2:417917/1‑150 (MQ=255)
                                                                                                                      aaGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAACTCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGAcc                                 <  2:1459615/150‑1 (MQ=255)
                                                                                                                      aaGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCCGACAATGGCCTGCGCGAcc                                 <  2:1823425/150‑1 (MQ=255)
                                                                                                                      aaGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGGCAATGGCCTGCGCGAcc                                 <  1:53579/150‑1 (MQ=255)
                                                                                                                      aaGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGAcc                                 <  1:1014704/150‑1 (MQ=255)
                                                                                                                       aGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGAcat                                >  2:408556/1‑148 (MQ=255)
                                                                                                                        gCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGCCAATGGCCTGCGCGACctc                               >  1:2161787/1‑150 (MQ=255)
                                                                                                                             aaGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTg                          <  2:723597/150‑1 (MQ=255)
                                                                                                                             aaGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTg                          >  2:1888953/1‑150 (MQ=255)
                                                                                                                              aGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGc                         >  1:1694896/1‑150 (MQ=255)
                                                                                                                              aGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGc                         >  1:2126128/1‑150 (MQ=255)
                                                                                                                               gTCGATGTAGTACCGGATGCAATTCTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGATTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTgtc                        <  2:1151723/150‑3 (MQ=255)
                                                                                                                                tCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGccg                       >  1:880580/1‑149 (MQ=255)
                                                                                                                                tCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCt                       >  1:948736/1‑150 (MQ=255)
                                                                                                                                 cGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTGACCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGGGACGTAAGAGGCTTTTTCGCTGAGTAGCCAGACAATGGCCTGCGAGACCTCTTCTGCCtt                      >  2:1442976/1‑149 (MQ=255)
                                                                                                                                    tGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAACTCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTcc                   <  2:1075157/150‑1 (MQ=255)
                                                                                                                                    tGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTcc                   >  1:1259107/1‑150 (MQ=255)
                                                                                                                                     gAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAAATTCGCTGATTTATTACCCGCACGCCAAATCGATAAAACTTCCGCTGACGTAAGAGGCTTTATAACTTAGTAGCCAGACAATGGCCTGCGCGACCTCGTCTGCCTTTcca                  >  2:1293663/1‑150 (MQ=255)
                                                                                                                                        tgaCCGGATGCAACAAGTATTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTccaccc               >  1:1881017/3‑149 (MQ=255)
                                                                                                                                        tgaCCGGATGCAACAAGTATTGCATCAGGTCAGACAACTTACCTTTTTTATTTCCCGCCCGCCAAATCGATAAAAATTCACGTGAAGTAAGAGGCTTGATCACTTAGTAGACAGACAATGGCCGGCGCGACCTAGTCTGCCTGTccacca               >  2:1721321/3‑150 (MQ=255)
                                                                                                                                           ccGGATGCAATACTTGTTTCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCGCGCCAAATAGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTGAGTAGCGAGACAATGGCCTTCGCGACCTCTTCTGCCTGTGAAACACGc            >  2:350105/1‑150 (MQ=255)
                                                                                                                                           ccGGATGCAATACTTGTTGCATCCGGTCAGACAACATCCCTGTTTTATTTCCCGCCCGACAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGc            >  2:350170/1‑150 (MQ=255)
                                                                                                                                           ccGGATGCAACAAGTATTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGc            >  1:431139/1‑150 (MQ=255)
                                                                                                                                           ccGGATGCAACAAGTATTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGc            >  1:1789415/1‑150 (MQ=255)
                                                                                                                                           ccGGATGCAACAAGTATTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGATTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACgg            >  2:954573/1‑149 (MQ=255)
                                                                                                                                             ggATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGCTg          >  2:1699325/1‑150 (MQ=255)
                                                                                                                                               aTGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGACTGCGCGACCTCTTCTGCCTGTCCACCACGCTGCa        >  1:2096138/1‑150 (MQ=255)
                                                                                                                                                 gCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGCTGCATg      >  1:591714/1‑150 (MQ=255)
                                                                                                                                                   aaTACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGCTGCATggg    <  2:1038476/150‑1 (MQ=255)
                                                                                                                                                    aTACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGCTGCATgggg   <  2:1335516/150‑1 (MQ=255)
                                                                                                                                                     tACTTGTTGCATCCGGTCAGACAACTACCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGCTGCATGGGGa  <  2:1853513/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CAGCAAACGCTACGGCTTTATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGCTGCATGGGGA  >  NC_000913/3044975‑3045273

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: