Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,045,129 G→A 7.9% intergenic (+28/+29) bglA → / ← ygfF 6‑phospho‑beta‑glucosidase A/putative NAD(P)‑dependent oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,045,1290GA 7.9% 342.1 / 12.1 113intergenic (+28/+29)bglA/ygfF6‑phospho‑beta‑glucosidase A/putative NAD(P)‑dependent oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (56/47);  new base A (5/4);  total (61/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.18e-01

Only 100 of 117 total aligned reads displayed.

AAACGCTACGGCTTTATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGCTGCATGGGGA  >  NC_000913/3044979‑3045273
                                                                                                                                                      |                                                                                                                                                
aaaCGCTACGGCTTTATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACttg                                                                                                                                                  >  2:2053703/1‑150 (MQ=255)
   cGCTACGGCTTTATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACttgtt                                                                                                                                                >  1:1029406/1‑150 (MQ=255)
    gCTACGGCTTTATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACttgttg                                                                                                                                               <  1:1614613/150‑1 (MQ=255)
    gCTACGGCTTTATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACttgttg                                                                                                                                               <  1:1443790/150‑1 (MQ=255)
       aCGGCTTTATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCAt                                                                                                                                            >  1:64182/1‑150 (MQ=255)
        cGGCTTTATCTATGTGAATAAACATGACGACCGTACTGTCGATATGTCGCGTTAACGTAAGAATAGCTTTATCTGGTACAAATAGGTGATTGACAGCAACGGCGAGAAGATTTAAGTCGATGAAATACCGGATTCAATACTAGTTGCAta                                                                                                                                           >  1:1075977/1‑149 (MQ=255)
         ggCTTTATCTATGTTAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGGGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAACAAGTATTGCATcc                                                                                                                                          <  1:1127550/150‑1 (MQ=255)
          gCTTTATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCGTTAACTGGTTAAAAGAGGTGATTGCCAGCAACGGCGAGCAGCTGTAAGTCGATGAAGTACCGGATGCAATACTAGTTGCATCCg                                                                                                                                         >  2:381450/1‑150 (MQ=255)
            tttATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAACAAGTATTGCATCCGGt                                                                                                                                       <  2:1145788/150‑1 (MQ=255)
              tATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCa                                                                                                                                     <  2:4515/150‑1 (MQ=255)
              tATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCa                                                                                                                                     <  1:2127200/150‑1 (MQ=255)
                tCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGa                                                                                                                                   >  1:150383/1‑150 (MQ=255)
                  tATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACa                                                                                                                                 >  1:1335516/1‑150 (MQ=255)
                  tATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTGTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACa                                                                                                                                 >  2:2071161/1‑150 (MQ=255)
                    tgtgAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCTGTCAGACaaa                                                                                                                               >  2:846564/1‑149 (MQ=255)
                    tgtgAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAAc                                                                                                                               <  1:566311/150‑1 (MQ=255)
                         aTAAACATCACGACGTTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTccc                                                                                                                          <  1:230142/150‑1 (MQ=255)
                              cATGACGACGGTACTGGCGATATGTCGCGTTCACGAAAGAAGAGCTTTAACTGGTACAAAGAGGCGATAGCCAGCAACGGCGAGAAGCTTTAAGTCGTTGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGttt                                                                                                                     <  1:1809200/150‑1 (MQ=255)
                                  acgacgGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTAtt                                                                                                                 <  2:1537369/150‑1 (MQ=255)
                                     acgGTACTGGCGATATGTCGCGTTCAGGTAAGAATAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGTAGCTTTAAGTCGATGAAGTACCGGCTGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTcc                                                                                                              <  1:1462438/150‑1 (MQ=255)
                                     acgGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTcc                                                                                                              <  2:1881771/150‑1 (MQ=255)
                                     acgGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTcc                                                                                                              <  2:1029406/150‑1 (MQ=255)
                                      cgGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTccc                                                                                                             >  1:932873/1‑150 (MQ=255)
                                           cTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTcccgcccg                                                                                                        <  2:1848982/150‑1 (MQ=255)
                                           cTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTcccgcccg                                                                                                        <  2:1273022/150‑1 (MQ=255)
                                             ggCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGATGCGTTAAGTCGATGAAGTACCGGAAGCAATAGTTGTTGCATCCCGTCAGACAACTTCCCTGTTGTATTTCCcgcccgcc                                                                                                      >  2:425443/1‑150 (MQ=255)
                                             ggCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCcgcccgcc                                                                                                      >  1:1919095/1‑150 (MQ=255)
                                             ggCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCcgcccgcc                                                                                                      >  1:2102100/1‑150 (MQ=255)
                                             ggCGATATGTCGCGTGCACGTAAGAAGCGCGGTAACTGGTACAAAGAGGTGATTGCCAGCACCGGCGAGAAGCTTTATGTCGATGAAGTACCGGATGCAATACGGGTTGCATCCGGTCAGACAACTTCGCTGTGTTATTTCCcgcccgcc                                                                                                      >  2:767076/1‑150 (MQ=255)
                                                  tatGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATc                                                                                                 <  1:554111/150‑1 (MQ=255)
                                                  tatGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATc                                                                                                 >  2:2164632/1‑150 (MQ=255)
                                                  tatGTCGCGGGCACGTAAGATGAGCTTTAACTGGTACAAAGAGGTGAGTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATc                                                                                                 <  2:377145/150‑1 (MQ=255)
                                                    tGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGa                                                                                               <  1:2189654/150‑1 (MQ=255)
                                                     gTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGAt                                                                                              >  1:1414779/1‑150 (MQ=255)
                                                     gTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGAt                                                                                              >  2:1918488/1‑150 (MQ=255)
                                                     gTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGAt                                                                                              >  1:636047/1‑150 (MQ=255)
                                                      tCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATa                                                                                             <  2:64182/150‑1 (MQ=255)
                                                       cgcgTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATaa                                                                                            >  2:625525/1‑150 (MQ=255)
                                                          gTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAAc                                                                                         <  1:1611718/150‑1 (MQ=255)
                                                             cACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTc                                                                                      >  1:1276646/1‑150 (MQ=255)
                                                                gTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCg                                                                                   >  2:2142199/1‑150 (MQ=255)
                                                                 tAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGAAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGt                                                                                  <  1:388582/150‑1 (MQ=255)
                                                                     aagaGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACg                                                                              <  1:1982655/150‑1 (MQ=255)
                                                                           tttAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAagag                                                                        >  1:2014494/1‑150 (MQ=255)
                                                                            ttAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAgg                                                                       >  1:1090748/1‑150 (MQ=255)
                                                                             tAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGc                                                                      >  2:166360/1‑150 (MQ=255)
                                                                             tAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGc                                                                      <  2:135925/150‑1 (MQ=255)
                                                                             tAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGc                                                                      <  1:1866580/150‑1 (MQ=255)
                                                                               aCTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCCTCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCGCGTGACGGAAGAGGCtt                                                                    >  2:230526/1‑150 (MQ=255)
                                                                               aCTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCtt                                                                    >  1:531671/1‑150 (MQ=255)
                                                                               aCTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCAGATGCAATACTTGTTGCATCCGTTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCtt                                                                    <  1:370203/150‑1 (MQ=255)
                                                                                 tGGTACAATGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATTGAATACTTGTTGCATGCGGTCAGACATCTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTGCCGTGACGTAAGAGGCTTTa                                                                  <  2:123012/150‑1 (MQ=255)
                                                                                  ggTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGAAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTAt                                                                 <  1:1330717/150‑1 (MQ=255)
                                                                                   gTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATc                                                                <  1:1681729/150‑1 (MQ=255)
                                                                                      cAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACt                                                             >  1:601541/1‑150 (MQ=255)
                                                                                      cAAAGACGTGATTGCCAGCAACGGCGAGAAGCTATAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCATGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACt                                                             >  1:1514479/1‑150 (MQ=255)
                                                                                       aaaGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACtt                                                            >  2:1978105/1‑150 (MQ=255)
                                                                                       aaaGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACtt                                                            <  2:907902/150‑1 (MQ=255)
                                                                                          gagGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACACCTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTtagt                                                         >  1:177057/1‑150 (MQ=255)
                                                                                          gagGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGAAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCACGTGACGTAAGAGGCTTTATCACTtagt                                                         >  1:1832598/1‑150 (MQ=255)
                                                                                             gTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGc                                                      >  1:979413/1‑150 (MQ=255)
                                                                                              tGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGcc                                                     <  1:1016697/150‑1 (MQ=255)
                                                                                              ggATTGCCAGCAACGGCGAGAAGCTTTAAGGCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGcc                                                     <  1:1773686/149‑1 (MQ=255)
                                                                                                aTTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAg                                                   <  2:2208566/150‑1 (MQ=255)
                                                                                                 ttGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGa                                                  >  2:1593509/1‑150 (MQ=255)
                                                                                                   gCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACATCTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGAGGTAAGAGGCTTTATCACTTAGTAGCCAGACa                                                >  2:1337789/1‑150 (MQ=255)
                                                                                                    ccAGCAACGGCGAGAAGCTTTAAGTCTATGAAGTACCGCATGCAATACTTGATGCATCCGGTCAGACAACGACCCTGTTTTATTTCCCGCCCGCCAACTCGATAAAAATTCCAGTCACGTAAGAGGCTTAATCACTTAGTAGCCAGACaa                                               >  1:345273/1‑150 (MQ=255)
                                                                                                       gCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCTGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAACCTTCCCGTGACGTATGAGGCTTTATCACTTAGTAGCCAGACAATgg                                            <  2:94959/150‑1 (MQ=255)
                                                                                                          aCGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCt                                         <  1:850269/150‑1 (MQ=255)
                                                                                                               gagaAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGcgcg                                    <  2:932873/150‑1 (MQ=255)
                                                                                                                  aaGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAACTCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGAcc                                 <  2:1459615/150‑1 (MQ=255)
                                                                                                                  aaGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCCGACAATGGCCTGCGCGAcc                                 <  2:1823425/150‑1 (MQ=255)
                                                                                                                  aaGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGAcc                                 <  1:1014704/150‑1 (MQ=255)
                                                                                                                   aGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGAcat                                >  2:408556/1‑148 (MQ=255)
                                                                                                                    gCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGCCAATGGCCTGCGCGACctc                               >  1:2161787/1‑150 (MQ=255)
                                                                                                                        tAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCtcttct                           >  1:1721041/1‑150 (MQ=255)
                                                                                                                         aaGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTg                          <  2:723597/150‑1 (MQ=255)
                                                                                                                         aaGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTg                          >  2:1888953/1‑150 (MQ=255)
                                                                                                                          aGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGc                         >  1:1694896/1‑150 (MQ=255)
                                                                                                                          aGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGc                         >  1:2126128/1‑150 (MQ=255)
                                                                                                                           gTCGATGTAGTACCGGATGCAATTCTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGATTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTgtc                        <  2:1151723/150‑3 (MQ=255)
                                                                                                                            tCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGccg                       >  1:880580/1‑149 (MQ=255)
                                                                                                                            tCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCt                       >  1:948736/1‑150 (MQ=255)
                                                                                                                             cGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTg                      >  1:1568299/1‑150 (MQ=255)
                                                                                                                                tGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAACTCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTcc                   <  2:1075157/150‑1 (MQ=255)
                                                                                                                                 gAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAAATTCGCTGATTTATTACCCGCACGCCAAATCGATAAAACTTCCGCTGACGTAAGAGGCTTTATAACTTAGTAGCCAGACAATGGCCTGCGCGACCTCGTCTGCCTTTcca                  >  2:1293663/1‑150 (MQ=255)
                                                                                                                                    tgaCCGGATGCAACAAGTATTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTccaccc               >  1:1881017/3‑149 (MQ=255)
                                                                                                                                    tgaCCGGATGCAACAAGTATTGCATCAGGTCAGACAACTTACCTTTTTTATTTCCCGCCCGCCAAATCGATAAAAATTCACGTGAAGTAAGAGGCTTGATCACTTAGTAGACAGACAATGGCCGGCGCGACCTAGTCTGCCTGTccacca               >  2:1721321/3‑150 (MQ=255)
                                                                                                                                     tACCGGATGCAATACTTTTTTCATACTGTCTTACAAATTCCCTGTTATAATTCAGGCCCGCCAAATCGATCAAACTTCCCTTGACGTCAGAGGCTTTAACACTTAGTAGACAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCcaccac              <  2:1337241/150‑1 (MQ=255)
                                                                                                                                       ccGGATGCAATACTTGTTTCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCGCGCCAAATAGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTGAGTAGCGAGACAATGGCCTTCGCGACCTCTTCTGCCTGTGAAACACGc            >  2:350105/1‑150 (MQ=255)
                                                                                                                                       ccGGATGCAATACTTGTTGCATCCGGTCAGACAACATCCCTGTTTTATTTCCCGCCCGACAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGc            >  2:350170/1‑150 (MQ=255)
                                                                                                                                       ccGGATGCAACAAGTATTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGc            >  1:431139/1‑150 (MQ=255)
                                                                                                                                       ccGGATGCAACAAGTATTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGc            >  1:1789415/1‑150 (MQ=255)
                                                                                                                                       ccGGATGCAACAAGTATTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGATTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACgg            >  2:954573/1‑149 (MQ=255)
                                                                                                                                         ggATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGCTg          >  2:1699325/1‑150 (MQ=255)
                                                                                                                                             gCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGCTGCATg      >  1:591714/1‑150 (MQ=255)
                                                                                                                                               attACTTGTTGCATCCGTTCAGACTACTTCCCTGTTTTATTTCCCGCCAGCCCCATCGCTCACAGTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGCCAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGCTGCATggg    <  1:381450/148‑1 (MQ=255)
                                                                                                                                               aaTACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGCTGCATggg    <  2:1038476/150‑1 (MQ=255)
                                                                                                                                                aTACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGCTGCATgggg   <  2:1335516/150‑1 (MQ=255)
                                                                                                                                                 tACTTGTTGCATCCGGTCAGACAACTACCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGCTGCATGGGGa  <  2:1853513/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                
AAACGCTACGGCTTTATCTATGTGAATAAACATGACGACGGTACTGGCGATATGTCGCGTTCACGTAAGAAGAGCTTTAACTGGTACAAAGAGGTGATTGCCAGCAACGGCGAGAAGCTTTAAGTCGATGAAGTACCGGATGCAATACTTGTTGCATCCGGTCAGACAACTTCCCTGTTTTATTTCCCGCCCGCCAAATCGATAAAACTTCCCGTGACGTAAGAGGCTTTATCACTTAGTAGCCAGACAATGGCCTGCGCGACCTCTTCTGCCTGTCCACCACGCTGCATGGGGA  >  NC_000913/3044979‑3045273

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: