Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,107,879 G→T 5.6% A426D (GCT→GAT) ‡ speC ← ornithine decarboxylase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,107,8790GT 5.6% 409.9 / 10.2 133A426D (GCT→GAT) ‡speCornithine decarboxylase, constitutive
Reads supporting (aligned to +/- strand):  ref base G (57/68);  new base T (4/4);  total (61/72)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.97e-01

Only 100 of 134 total aligned reads displayed.

ATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGCATAAAGGCGTTATTC  >  NC_000913/3107730‑3108025
                                                                                                                                                     |                                                                                                                                                  
atCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATACTCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAg                                                                                                                                                    <  1:1781581/150‑1 (MQ=255)
  ccTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCAGCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGcc                                                                                                                                                  >  2:1270241/1‑150 (MQ=255)
   cTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAACCGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCt                                                                                                                                                 >  2:389163/1‑150 (MQ=255)
      cAAAGCCGCGCCACTTAGCCCCCGGCTCAAAACTGAAAAAACGGCTGTCGCTGGCTACCACGGATGTCTGATAAGCCTGCCACACTTTGCCATCCACCACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTgc                                                                                                                                              <  1:119406/150‑1 (MQ=255)
       aaaGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTgcg                                                                                                                                             >  1:535643/1‑150 (MQ=255)
        aaGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTgcgc                                                                                                                                            <  2:614750/150‑1 (MQ=255)
          gCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCGATCAACAACGGGCGGGATAAACGGGCGGACCAGCTTACAGCGCGCAAGAAGAGCCTTgcgcgc                                                                                                                                          >  1:1712161/1‑150 (MQ=255)
            cGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGGCTATTCTTGCGCGCtg                                                                                                                                        <  2:75680/150‑2 (MQ=255)
            cGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGGCTATTCTTGCGCGCtg                                                                                                                                        <  2:850326/150‑2 (MQ=255)
            cGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCtt                                                                                                                                        <  2:1244817/150‑1 (MQ=255)
             gTGCCTCTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGTTCTCTGGCTAACTCTGATGGCGGATAATCCTGCCACAAATTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTc                                                                                                                                       <  2:2113399/150‑1 (MQ=255)
               gCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACCGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCaa                                                                                                                                     >  2:373988/1‑150 (MQ=255)
                ccACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTCAGGTCGGATAATCCGGCCACACTTTGCCATCAACAACGGGCGGGAGACACGGGCGGAACAGCTTACAGCGCGCATGAATAGCCTTGCGCGCTTCaaa                                                                                                                                    >  1:280127/1‑149 (MQ=255)
                      cGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTAt                                                                                                                              >  1:820457/1‑150 (MQ=255)
                       gCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTAtc                                                                                                                             >  1:1438689/1‑150 (MQ=255)
                        cccccGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTAtct                                                                                                                            >  1:971688/1‑150 (MQ=255)
                        cccccGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTAtct                                                                                                                            <  1:234528/150‑1 (MQ=255)
                         ccccGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTAtctc                                                                                                                           >  1:1091885/1‑150 (MQ=255)
                          cccGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCa                                                                                                                          <  1:123483/150‑1 (MQ=255)
                          cccGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCGAACCCTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCa                                                                                                                          <  1:129057/150‑1 (MQ=255)
                            cGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAac                                                                                                                        >  2:1129246/1‑150 (MQ=255)
                            cGGATCAAAACTGAAAAAAAGGCGGTCGCTGGCTAACAATGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAac                                                                                                                        <  2:196730/150‑1 (MQ=255)
                              gCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAacac                                                                                                                      >  2:1848811/1‑150 (MQ=255)
                              gCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAacac                                                                                                                      >  1:2073009/1‑150 (MQ=255)
                               cTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAacaca                                                                                                                     >  2:849179/1‑150 (MQ=255)
                                 cAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACt                                                                                                                   <  1:1838775/150‑1 (MQ=255)
                                      cTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGcc                                                                                                              >  2:1220102/1‑150 (MQ=255)
                                         aaaaaaCGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCcac                                                                                                           <  2:627600/150‑1 (MQ=255)
                                           aaaaCGGCGGTCGCTGTCTAAAACAGATGTCGGATAATACTGGCACTATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACATCGGGCAATAATAGCCTTTCGCGCTTGAATCCCTATCTCAACACACTCAGCCCACAg                                                                                                         <  2:501102/150‑1 (MQ=255)
                                           aaaaCGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACTGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCcacac                                                                                                         >  2:1752251/1‑149 (MQ=255)
                                               cggcggTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGa                                                                                                     <  1:952445/150‑1 (MQ=255)
                                                  cggTCGCTGGCTAACACTGATGTCGGATGATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGc                                                                                                  <  1:554038/150‑1 (MQ=255)
                                                    gTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGCCGccc                                                                                                >  1:1522889/1‑150 (MQ=255)
                                                        cTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACCCACTCAGCCCACAGCCGACGCCCActc                                                                                            >  2:206182/1‑150 (MQ=255)
                                                             tAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTcccc                                                                                       >  2:1244060/1‑150 (MQ=255)
                                                              aaCACTGATGTCGGATAATCCTGCCCCAATTTGCCATCAACAACGGGCGGGATAAACGGGAGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGATTCAATCCCTATCTCAACACACTCAGCCCCCAGCCCACGCACACTCTCCCCt                                                                                      >  2:1049539/1‑150 (MQ=255)
                                                              aaCACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACCGCCGACGCCCACTCTCCCCt                                                                                      >  1:1968293/1‑150 (MQ=255)
                                                                aacTGATGTCGGATAATACTGCCACATTTTGTCATCAACATCGGGCGGGATAAACGGGCGGAACAGCTTACTGCGCGCAAGAATAGCCTTGCGCTCTTCATTCGCTATCTCTACACACTCATCCCACAGCCGACGCCCACTCTCCCCTTc                                                                                    <  2:939802/149‑1 (MQ=255)
                                                                 acTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCa                                                                                   <  1:1988438/150‑1 (MQ=255)
                                                                  cTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACATCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCAt                                                                                  <  2:1710583/150‑1 (MQ=255)
                                                                     aTGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGaa                                                                               <  1:1029519/150‑1 (MQ=255)
                                                                         cGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACCGCCGACGCCCCCTCTCCCCTTCCTGAAtttt                                                                           >  2:1701229/1‑150 (MQ=255)
                                                                         cGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAAtttt                                                                           <  1:963172/150‑1 (MQ=255)
                                                                          tgATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCATTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTg                                                                          <  2:389895/149‑1 (MQ=255)
                                                                          ggATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTg                                                                          <  1:1151965/150‑1 (MQ=255)
                                                                            aTAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGc                                                                        <  1:1355592/150‑1 (MQ=255)
                                                                             tAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCg                                                                       <  1:354025/150‑1 (MQ=255)
                                                                             tAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCg                                                                       <  1:152385/150‑1 (MQ=255)
                                                                              aaTCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGt                                                                      <  2:56597/150‑1 (MQ=255)
                                                                                   tGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACa                                                                 <  2:1682942/150‑1 (MQ=255)
                                                                                   tGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACa                                                                 >  2:1945678/1‑150 (MQ=255)
                                                                                    gCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACAt                                                                <  2:1997043/150‑1 (MQ=255)
                                                                                     ccACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGTAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTACAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATc                                                               >  1:1450301/1‑150 (MQ=255)
                                                                                     ccACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACGCTCCCCTGCATGAATTTTGGCGTTAACATc                                                               >  2:477067/1‑150 (MQ=255)
                                                                                      cacaATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATcc                                                              <  2:2007164/150‑1 (MQ=255)
                                                                                        caATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAg                                                            <  2:820457/150‑1 (MQ=255)
                                                                                        caATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAg                                                            <  1:1716404/150‑1 (MQ=255)
                                                                                           tttGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTgc                                                         <  2:944051/150‑1 (MQ=255)
                                                                                           tttGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTgc                                                         <  1:858/150‑1 (MQ=255)
                                                                                           tttGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTgc                                                         <  2:210692/150‑1 (MQ=255)
                                                                                             tGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTgcag                                                       >  2:925182/1‑150 (MQ=255)
                                                                                               ccATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTgcagca                                                     >  1:463913/1‑150 (MQ=255)
                                                                                               ccATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTgcagca                                                     <  1:849179/150‑1 (MQ=255)
                                                                                                cATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCaa                                                    >  1:1029652/1‑150 (MQ=255)
                                                                                                  tCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAAc                                                  <  2:726280/150‑1 (MQ=255)
                                                                                                  tCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAAc                                                  <  2:1150954/150‑1 (MQ=255)
                                                                                                     acaacGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCTCAGCCGACGCCCACTCTCCCCCTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGc                                               <  2:313909/150‑1 (MQ=255)
                                                                                                      caacGGGCGGGATAAACGGGCGGAACAGCTTACCGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCg                                              <  1:372286/150‑1 (MQ=255)
                                                                                                      caacGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCg                                              <  1:389163/150‑1 (MQ=255)
                                                                                                       aacGGGCGGGATAAACGTGCGGAACAGCTTACAGCGCGAAATAATAGCCATGCGCGATTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCgg                                             <  2:400583/150‑1 (MQ=255)
                                                                                                       aacGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCgg                                             <  1:2029471/150‑1 (MQ=255)
                                                                                                       aacGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCgg                                             <  1:850325/150‑1 (MQ=255)
                                                                                                           ggcgggATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACCCACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAg                                         <  2:1010146/150‑1 (MQ=255)
                                                                                                           ggcgggATAAACGGGCGGAACAGATTACAGCGAGCAAGAATAGCCTTGCTCGCTTCAATCACTATCTCAAAACACTCATCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAg                                         <  2:1685816/150‑1 (MQ=255)
                                                                                                            gcgggATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCGTCATGAAGTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAgc                                        >  2:1961050/1‑149 (MQ=255)
                                                                                                               ggATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACTTCCAGTGCAGCAAACAGCGGATAGAAAg                                     <  1:1844181/150‑1 (MQ=255)
                                                                                                                   aaaCGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCt                                 <  2:1089683/150‑1 (MQ=255)
                                                                                                                       gggCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTa                             <  2:1091885/150‑1 (MQ=255)
                                                                                                                           ggAACAGCTTACAGCGCGAAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAg                         <  2:1438689/150‑1 (MQ=255)
                                                                                                                            gAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCGGGTAGAAGc                        >  1:148494/1‑150 (MQ=255)
                                                                                                                            gAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATACCTATCTCCACACACTCAGCCCGCCGCCGACGCCCCCGCTCCCCGTCATGAAGTTTGGCGTTAACCGCCCGTGCAGCAAGCAGCGGAGAGGACTGGCTGGTAGAAGc                        >  1:1928194/1‑150 (MQ=255)
                                                                                                                             aaCAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCa                       <  2:474765/150‑1 (MQ=255)
                                                                                                                              aCAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTGCATGAATTTTGGCGTTAACATCCAGTGCCGCAACCAGCCGATAGAAAGGGCTGGTAGAAGCAt                      >  1:337660/1‑150 (MQ=255)
                                                                                                                              aCAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCATCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCAt                      >  1:64622/1‑150 (MQ=255)
                                                                                                                                 gCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGCCGCCCACTCTCCCCATCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGAGAGAAAGGGCTGGTAGCAGCATGGa                   >  1:357255/1‑150 (MQ=255)
                                                                                                                                 gCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCGGCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGa                   >  1:1808965/1‑150 (MQ=255)
                                                                                                                                  cTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAg                  >  1:2182137/1‑150 (MQ=255)
                                                                                                                                    tACAGCTCGCAAGAATAGCCTTGCGCGCGTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGCa                <  2:1195652/150‑1 (MQ=255)
                                                                                                                                      cAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGCATa              >  2:1526674/1‑150 (MQ=255)
                                                                                                                                       aGCGCGCAAGGCTATTCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACCGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGCAtta             >  2:317066/1‑148 (MQ=255)
                                                                                                                                       aGCGCGCAAGGCTATTCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCTTGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGCATaa             >  2:915172/1‑150 (MQ=255)
                                                                                                                                       aGCGCGCAAGGCTATTCTTGCGCGCTTCAATCCCTATCTAAACACACACAGCCCACAGCCGACGCCCACTATACCCTACAAGAATTTTCGCGTTAACATACAGTCCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATCGAGCATaa             >  1:222821/1‑150 (MQ=255)
                                                                                                                                       aGCGCGCAAGGCTATTCTTGCGCGCTTCAAGCCCTATCTCACCACACTCAGCCCACAGCCGACGCCCACTCTCCCCGGCATGAATTTTGGCGTTAACATCCAGTGGCGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGCGCATaa             >  2:1354371/1‑150 (MQ=255)
                                                                                                                                        gcgcgcAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGCATaaa            >  2:810216/1‑150 (MQ=255)
                                                                                                                                           cgcAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGCATAAAGGc         >  2:902338/1‑150 (MQ=255)
                                                                                                                                            gcAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGCATAGAGGCg        <  1:778700/150‑1 (MQ=255)
                                                                                                                                             cAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGCATAAAGGCGt       >  2:2171607/1‑150 (MQ=255)
                                                                                                                                               aGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGCATAAAGGCGTTa     <  1:1300395/150‑1 (MQ=255)
                                                                                                                                                gAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCACACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGCATAAAGGCggttt    >  1:803435/1‑146 (MQ=255)
                                                                                                                                                  aTAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCGTCATGAATTTTGGCGTTACCATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGCATAAAGGCGTTATTc  >  1:1458617/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
ATCCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGCATAAAGGCGTTATTC  >  NC_000913/3107730‑3108025

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: