Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,107,880 C→T 5.3% A426T (GCT→ACT) ‡ speC ← ornithine decarboxylase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,107,8800CT 5.3% 424.0 / 7.5 132A426T (GCT→ACT) ‡speCornithine decarboxylase, constitutive
Reads supporting (aligned to +/- strand):  ref base C (56/69);  new base T (4/3);  total (60/72)
Fisher's exact test for biased strand distribution p-value = 7.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.33e-01

Only 100 of 133 total aligned reads displayed.

CCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGCATAAAGGCGTTATTC  >  NC_000913/3107732‑3108025
                                                                                                                                                    |                                                                                                                                                 
 cTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAACCGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCt                                                                                                                                                 >  2:389163/1‑150 (MQ=255)
    cAAAGCCGCGCCACTTAGCCCCCGGCTCAAAACTGAAAAAACGGCTGTCGCTGGCTACCACGGATGTCTGATAAGCCTGCCACACTTTGCCATCCACCACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTgc                                                                                                                                              <  1:119406/150‑1 (MQ=255)
     aaaGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTgcg                                                                                                                                             >  1:535643/1‑150 (MQ=255)
      aaGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTgcgc                                                                                                                                            <  2:614750/150‑1 (MQ=255)
        cccGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGTCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCTCAAGGCTATTCTTgcgcgc                                                                                                                                          <  2:1673265/149‑1 (MQ=255)
          cGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGGCTATTCTTGCGCGCtg                                                                                                                                        <  2:75680/150‑2 (MQ=255)
          cGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCtt                                                                                                                                        <  2:1244817/150‑1 (MQ=255)
           gTGCCTCTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGTTCTCTGGCTAACTCTGATGGCGGATAATCCTGCCACAAATTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTc                                                                                                                                       <  2:2113399/150‑1 (MQ=255)
              ccACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTCAGGTCGGATAATCCGGCCACACTTTGCCATCAACAACGGGCGGGAGACACGGGCGGAACAGCTTACAGCGCGCATGAATAGCCTTGCGCGCTTCaaa                                                                                                                                    >  1:280127/1‑149 (MQ=255)
                  ttCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATcccg                                                                                                                                >  2:1146660/1‑149 (MQ=255)
                    cGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTAt                                                                                                                              >  1:820457/1‑150 (MQ=255)
                     gCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTAtc                                                                                                                             >  1:489974/1‑150 (MQ=255)
                      cccccGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTAtct                                                                                                                            >  1:971688/1‑150 (MQ=255)
                       ccccGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTAtctc                                                                                                                           >  1:1091885/1‑150 (MQ=255)
                        cccGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCa                                                                                                                          <  1:123483/150‑1 (MQ=255)
                          cGGCTCAAAACTGATAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTTCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACATGTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAac                                                                                                                        <  2:1236413/150‑1 (MQ=255)
                             cTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAacaca                                                                                                                     >  2:849179/1‑150 (MQ=255)
                                        aaaaaCGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCCACACACTCAGCCcaca                                                                                                          >  1:1859216/1‑150 (MQ=255)
                                         aaaaCGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACTGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCcacac                                                                                                         >  2:1752251/1‑149 (MQ=255)
                                            aCGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCg                                                                                                      <  2:1937174/150‑1 (MQ=255)
                                            aCGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAAAGGGAGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCg                                                                                                      <  1:1361419/150‑1 (MQ=255)
                                             cggcggTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGa                                                                                                     <  1:952445/150‑1 (MQ=255)
                                             cggcggTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGa                                                                                                     >  2:929462/1‑150 (MQ=255)
                                                 ggTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGcc                                                                                                 >  1:870022/1‑150 (MQ=255)
                                                 ggTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGcc                                                                                                 <  2:559592/150‑1 (MQ=255)
                                                 ggTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACACCCGACGcc                                                                                                 <  1:2190488/150‑1 (MQ=255)
                                                  gTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGCCGccc                                                                                                >  1:1522889/1‑150 (MQ=255)
                                                  gTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGccc                                                                                                >  1:1544235/1‑150 (MQ=255)
                                                     gCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCAct                                                                                             <  2:820236/150‑1 (MQ=255)
                                                           tAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTcccc                                                                                       >  1:1999715/1‑150 (MQ=255)
                                                           tAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTcccc                                                                                       >  2:1244060/1‑150 (MQ=255)
                                                              cacTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTc                                                                                    <  2:720794/150‑1 (MQ=255)
                                                              aacTGATGTCGGATAATACTGCCACATTTTGTCATCAACATCGGGCGGGATAAACGGGCGGAACAGCTTACTGCGCGCAAGAATAGCCTTGCGCTCTTCATTCGCTATCTCTACACACTCATCCCACAGCCGACGCCCACTCTCCCCTTc                                                                                    <  2:939802/149‑1 (MQ=255)
                                                               acTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCa                                                                                   <  1:1988438/150‑1 (MQ=255)
                                                                cTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACATCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCAt                                                                                  <  2:1710583/150‑1 (MQ=255)
                                                                  gATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGa                                                                                >  1:1798369/1‑150 (MQ=255)
                                                                   aTGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGaa                                                                               <  1:1029519/150‑1 (MQ=255)
                                                                        tgATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCATTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTg                                                                          <  2:389895/149‑1 (MQ=255)
                                                                        ggATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGACGACGCCCACTCTCCCCTTCATGAATTTTg                                                                          >  1:362483/1‑150 (MQ=255)
                                                                          aTAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGc                                                                        <  1:1355592/150‑1 (MQ=255)
                                                                           tAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCg                                                                       <  1:152385/150‑1 (MQ=255)
                                                                            aaTCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGt                                                                      >  1:689695/1‑150 (MQ=255)
                                                                            aaTCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGt                                                                      <  2:56597/150‑1 (MQ=255)
                                                                                cTGCCACAATTTGCCATCCACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAAc                                                                  <  2:1065690/150‑1 (MQ=255)
                                                                                 tGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACa                                                                 >  2:1945678/1‑150 (MQ=255)
                                                                                 tGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACa                                                                 <  2:1682942/150‑1 (MQ=255)
                                                                                  gCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACAt                                                                <  2:1997043/150‑1 (MQ=255)
                                                                                   ccACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGTAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTACAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATc                                                               >  1:1450301/1‑150 (MQ=255)
                                                                                   ccACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACGCTCCCCTGCATGAATTTTGGCGTTAACATc                                                               >  2:477067/1‑150 (MQ=255)
                                                                                    cacaATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATcc                                                              <  2:2007164/150‑1 (MQ=255)
                                                                                    cacaATATGCCATCAACAACGCGCGGGATAAACGGGCGGAACAGCTGACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCATTCATGAATTTTTGCGTTAACATcc                                                              <  1:331252/150‑1 (MQ=255)
                                                                                     acaATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCa                                                             >  2:1300395/1‑150 (MQ=255)
                                                                                      caATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAg                                                            <  1:1716404/150‑1 (MQ=255)
                                                                                      caATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAg                                                            <  2:820457/150‑1 (MQ=255)
                                                                                         tttGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTgc                                                         <  1:858/150‑1 (MQ=255)
                                                                                         tttGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTgc                                                         <  2:210692/150‑1 (MQ=255)
                                                                                         tttGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTgc                                                         <  2:944051/150‑1 (MQ=255)
                                                                                             ccATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTgcagca                                                     >  1:463913/1‑150 (MQ=255)
                                                                                             ccATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTgcagca                                                     <  1:849179/150‑1 (MQ=255)
                                                                                              cATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCaa                                                    >  1:1029652/1‑150 (MQ=255)
                                                                                                tCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAAc                                                  <  2:726280/150‑1 (MQ=255)
                                                                                                tCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAAc                                                  <  2:1150954/150‑1 (MQ=255)
                                                                                                   acaacGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCTCAGCCGACGCCCACTCTCCCCCTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGc                                               <  2:313909/150‑1 (MQ=255)
                                                                                                   acaacGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGc                                               >  2:19955/1‑150 (MQ=255)
                                                                                                    caacGGGCGGGATAAACGGGCGGAACAGCTTACCGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCg                                              <  1:372286/150‑1 (MQ=255)
                                                                                                     aacGGGCGGGTTAAACGGGCGGAACAGCTTACAGCGCGCAAGAATATCCTTGCGCGCTTAAATCCCTATCTCAACACACTCCGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCgg                                             <  2:901783/150‑1 (MQ=255)
                                                                                                     aacGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCgg                                             <  1:981963/150‑1 (MQ=255)
                                                                                                     aacGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCgg                                             <  1:2029471/150‑1 (MQ=255)
                                                                                                         ggcgggATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACCCACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAg                                         <  2:1010146/150‑1 (MQ=255)
                                                                                                         ggcgggATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACCCTCAGCCCACAGCCGACGCCCACTCTCCCCGTCAGGAATTTTGGCGTTACCATCCAGTGCAGCAAACAGCGGCTAg                                         >  1:1478407/1‑150 (MQ=255)
                                                                                                         ggcgggATAAACGGGCGGAACAGATTACAGCGAGCAAGAATAGCCTTGCTCGCTTCAATCACTATCTCAAAACACTCATCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAg                                         <  2:1685816/150‑1 (MQ=255)
                                                                                                          gcgggATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCGTCATGAAGTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAgc                                        >  2:1961050/1‑149 (MQ=255)
                                                                                                             ggATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACTTCCAGTGCAGCAAACAGCGGATAGAAAg                                     <  1:1844181/150‑1 (MQ=255)
                                                                                                                 aaaCGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCt                                 <  2:1089683/150‑1 (MQ=255)
                                                                                                                    cGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTACAGCAAACAGCGGATAGAAAGGGCTGGt                              <  2:2073009/150‑1 (MQ=255)
                                                                                                                     gggCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTa                             <  2:1091885/150‑1 (MQ=255)
                                                                                                                     gggCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCACCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTa                             <  1:1102448/150‑1 (MQ=255)
                                                                                                                      ggCGGAACAGCTTACAGCGCGCAAGAATAGCCTAGCGCGCTTCAATACCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAg                            <  1:1270241/150‑1 (MQ=255)
                                                                                                                         ggAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAg                         <  2:37826/150‑1 (MQ=255)
                                                                                                                         ggAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAg                         <  2:1709803/150‑1 (MQ=255)
                                                                                                                         ggAACAGCTTACAGCGCGAAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAg                         <  2:1438689/150‑1 (MQ=255)
                                                                                                                          gAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATACCTATCTCCACACACTCAGCCCGCCGCCGACGCCCCCGCTCCCCGTCATGAAGTTTGGCGTTAACCGCCCGTGCAGCAAGCAGCGGAGAGGACTGGCTGGTAGAAGc                        >  1:1928194/1‑150 (MQ=255)
                                                                                                                           aaCAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCa                       <  2:474765/150‑1 (MQ=255)
                                                                                                                            aCAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTGCATGAATTTTGGCGTTAACATCCAGTGCCGCAACCAGCCGATAGAAAGGGCTGGTAGAAGCAt                      >  1:337660/1‑150 (MQ=255)
                                                                                                                            aCAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCATCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCAt                      >  1:64622/1‑150 (MQ=255)
                                                                                                                               gCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGCCGCCCACTCTCCCCATCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGAGAGAAAGGGCTGGTAGCAGCATGGa                   >  1:357255/1‑150 (MQ=255)
                                                                                                                               gCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCGGCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGa                   >  1:1808965/1‑150 (MQ=255)
                                                                                                                                cTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAg                  >  1:2182137/1‑150 (MQ=255)
                                                                                                                                  tACAGCTCGCAAGAATAGCCTTGCGCGCGTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGCa                <  2:1195652/150‑1 (MQ=255)
                                                                                                                                    cAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGCATa              >  1:841666/1‑150 (MQ=255)
                                                                                                                                     aGCGCGCAAGGCTATTCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACCGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGCAtta             >  2:317066/1‑148 (MQ=255)
                                                                                                                                     aGCGCGCAAGGCTATTCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCTTGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGCATaa             >  2:915172/1‑150 (MQ=255)
                                                                                                                                     aGCGCGCAAGGCTATTCTTGCGCGCTTCAATCCCTATCTAAACACACACAGCCCACAGCCGACGCCCACTATACCCTACAAGAATTTTCGCGTTAACATACAGTCCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATCGAGCATaa             >  1:222821/1‑150 (MQ=255)
                                                                                                                                     aGCGCGCAAGGCTATTCTTGCGCGCTTCAAGCCCTATCTCACCACACTCAGCCCACAGCCGACGCCCACTCTCCCCGGCATGAATTTTGGCGTTAACATCCAGTGGCGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGCGCATaa             >  2:1354371/1‑150 (MQ=255)
                                                                                                                                      gcgcgcAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGCATaaa            >  2:260203/1‑150 (MQ=255)
                                                                                                                                         cgcAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGCATAAAGGc         >  2:902338/1‑150 (MQ=255)
                                                                                                                                          gcAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGCATAGAGGCg        <  1:778700/150‑1 (MQ=255)
                                                                                                                                             aGAATAGCCTTTCGCGCTTCAATCCCTATCTCAAAACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGCATAAAGGCGTTa     <  2:413315/150‑1 (MQ=255)
                                                                                                                                             aGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGCATAAAGGCGTTa     <  1:1300395/150‑1 (MQ=255)
                                                                                                                                                aTAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCGTCATGAATTTTGGCGTTACCATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGCATAAAGGCGTTATTc  >  1:1458617/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
CCTTCAAAGCCGTGCCACTTCGCCCCCGGCTCAAAACTGAAAAAACGGCGGTCGCTGGCTAACACTGATGTCGGATAATCCTGCCACAATTTGCCATCAACAACGGGCGGGATAAACGGGCGGAACAGCTTACAGCGCGCAAGAATAGCCTTGCGCGCTTCAATCCCTATCTCAACACACTCAGCCCACAGCCGACGCCCACTCTCCCCTTCATGAATTTTGGCGTTAACATCCAGTGCAGCAAACAGCGGATAGAAAGGGCTGGTAGAAGCATGGAGCATAAAGGCGTTATTC  >  NC_000913/3107732‑3108025

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: