Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 209,611 G→A 6.8% intergenic (+31/‑68) accA → / → ldcC acetyl‑CoA carboxylase, carboxytransferase, alpha subunit/lysine decarboxylase 2, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913209,6110GA 6.8% 358.6 / 10.6 117intergenic (+31/‑68)accA/ldcCacetyl‑CoA carboxylase, carboxytransferase, alpha subunit/lysine decarboxylase 2, constitutive
Reads supporting (aligned to +/- strand):  ref base G (47/62);  new base A (4/4);  total (51/66)
Fisher's exact test for biased strand distribution p-value = 7.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.91e-01

Only 100 of 119 total aligned reads displayed.

CGATGGCGGCATCGTTGAAAGCGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAGCCCATCAAAGAACTGGAGTCGGCGCTGGTGG  >  NC_000913/209462‑209760
                                                                                                                                                     |                                                                                                                                                     
cGATGGCGGCATCGTTGAAAGCGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCg                                                                                                                                                       >  2:2137290/1‑150 (MQ=255)
    ggcggcATCGTTGAAAGCGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTgg                                                                                                                                                   <  1:1735201/150‑1 (MQ=255)
    ggcggcATCGTTGAAAGCGAAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTGCTGAAAAAGGGTCACTTCGGTgg                                                                                                                                                   <  2:514593/150‑1 (MQ=255)
     gcggcATCGTTGAAAGCGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGc                                                                                                                                                  >  1:1549006/1‑150 (MQ=255)
         cATCGTTGAAAGCGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCtt                                                                                                                                              >  2:761866/1‑150 (MQ=255)
           tCGTTGAAAGCGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCtttt                                                                                                                                            <  2:138978/150‑1 (MQ=255)
              ttGAAAGCGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGCCACCGAAGTGACCCtttttca                                                                                                                                         <  1:535184/150‑3 (MQ=255)
               tGAAAGCGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGCCACCGAAGTGACCCtttttcag                                                                                                                                        <  1:1694727/150‑4 (MQ=255)
                gAAAGCGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCATCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATc                                                                                                                                       >  2:345707/1‑150 (MQ=255)
                  tagagcAACTGGTGGCGGTTCTGGCAGATGACGACGTGTTAAGCACTGATTATTTAAAATATCGTAGTTTTCAGCGCCTGATGAGCTACGGTAACGCGCAATTAGCAATAGTTCTGAAAAAGGGTCACTTAGGTTGCCCTTTTTTATCGc                                                                                                                                     <  2:408722/146‑1 (MQ=255)
                     cgcAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGCTGCGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTGTTTTTCGCCAc                                                                                                                                  >  2:1779828/1‑150 (MQ=255)
                        aaCTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGt                                                                                                                               <  2:706050/150‑1 (MQ=255)
                          ctgctgGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGttt                                                                                                                             <  2:892863/150‑1 (MQ=255)
                           tgctgGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGTGCTACGGATACGCGTAATTCGCAAAAGTTCTGAAAAAGGGGCACTTCGGTGGCCCTTTTTTATCGCCTCGGTTTg                                                                                                                            <  1:1863422/150‑1 (MQ=255)
                            gctgGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGa                                                                                                                           <  2:1456216/150‑1 (MQ=255)
                            gctgGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGa                                                                                                                           >  2:406173/1‑150 (MQ=255)
                             cttccGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCTTAATTCGCAAAAGTTCTGAAAAAGTGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAg                                                                                                                          <  1:1677109/146‑1 (MQ=255)
                             ctgGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCGTTTTTATCGCCACGGTTTGAg                                                                                                                          >  1:1396700/1‑150 (MQ=255)
                               ggCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGACCTTTTTTATCGCGGCGGTTTGAGCa                                                                                                                        >  2:313528/1‑150 (MQ=255)
                                 cGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTTCGCCACGGTTTGAGCAgga                                                                                                                     >  2:1157012/1‑149 (MQ=255)
                                 cGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTATCGCCACGGTTTGAGCAGGc                                                                                                                     >  2:1346272/1‑150 (MQ=255)
                                  ggATCTGGCCGATCTCGACGTGTTAAGCAATGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGc                                                                                                                     <  2:1217764/150‑1 (MQ=255)
                                    aTCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTa                                                                                                                   <  1:391238/150‑1 (MQ=255)
                                    aTCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTa                                                                                                                   <  1:2041994/150‑1 (MQ=255)
                                     tCTGGCCGTTCTCGACGTGTTCAGCCCTGACGAATTAAAAAATCGTCGTTATCAGAGCCTGATGAGCTACGGGTACGCGTAATTCGAAAAAGTTCTGAAAAAGGGTCACATCGGTGGCCCTTTTTTATCTCCACGGTTTGAGCAGGCTAt                                                                                                                  <  1:1155582/150‑1 (MQ=255)
                                      cTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATg                                                                                                                 <  2:1166583/150‑1 (MQ=255)
                                      cTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATg                                                                                                                 >  2:2046818/1‑150 (MQ=255)
                                       tGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAATGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGa                                                                                                                <  1:1268792/150‑1 (MQ=255)
                                        ggCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGAt                                                                                                               >  1:609035/1‑150 (MQ=255)
                                          ccGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTa                                                                                                             >  2:645816/1‑150 (MQ=255)
                                          ccGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTa                                                                                                             >  2:1854195/1‑150 (MQ=255)
                                          ccGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTa                                                                                                             <  2:1555007/150‑1 (MQ=255)
                                            gATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTaag                                                                                                           <  2:1314215/150‑1 (MQ=255)
                                            gATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTaag                                                                                                           <  2:1491260/150‑1 (MQ=255)
                                              tctcGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTaagga                                                                                                         <  2:1047444/150‑1 (MQ=255)
                                                tcGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTaaggaag                                                                                                       >  1:2149792/1‑150 (MQ=255)
                                                   aCGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGAt                                                                                                    <  2:1977886/150‑1 (MQ=255)
                                                       gtTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGAGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTATTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTc                                                                                                >  1:1575573/1‑150 (MQ=255)
                                                          aaGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCTAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTGTGAGCATGCTATGATTAAGGAAGGATTTTCCag                                                                                             <  1:1525719/150‑1 (MQ=255)
                                                          aaGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCag                                                                                             >  1:1229820/1‑150 (MQ=255)
                                                             cACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCaggag                                                                                          <  2:246176/150‑1 (MQ=255)
                                                             cACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCaggag                                                                                          <  1:1767783/150‑1 (MQ=255)
                                                               cTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCCCGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAggagga                                                                                        >  2:1185723/1‑150 (MQ=255)
                                                               cTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGAAAAAGTTCTGAAAAAGGGTCACTTCGTTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTACAggagga                                                                                        <  1:1562812/150‑1 (MQ=255)
                                                               cTGAAGATTTAAAAAATCGTCGTTATCAGCGCCCGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAGGATTTTCCAGGAGGaa                                                                                       >  1:828423/1‑150 (MQ=255)
                                                                 gAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAac                                                                                      >  1:2124006/1‑150 (MQ=255)
                                                                 gAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGCCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCAAGGAGGAac                                                                                      >  2:1226681/1‑150 (MQ=255)
                                                                  aaGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAaca                                                                                     <  2:1549006/150‑1 (MQ=255)
                                                                   aGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAacac                                                                                    <  1:2205712/150‑1 (MQ=255)
                                                                    gATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAAcaca                                                                                   >  2:1770965/1‑150 (MQ=255)
                                                                      tttAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCGTTTTTATCGCCACGGTTTGAGCAGGCTATGAGTAAGGAAGGATTTTCCAGGAGGAACACATg                                                                                 >  2:1065575/1‑150 (MQ=255)
                                                                        tAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGaa                                                                               >  1:1811259/1‑150 (MQ=255)
                                                                         aaaaaaTCGTCGGTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAAc                                                                              <  2:1205146/150‑1 (MQ=255)
                                                                           aaaaTCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAAcat                                                                            >  2:634804/1‑150 (MQ=255)
                                                                            aaaTCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAAcatc                                                                           >  1:489474/1‑150 (MQ=255)
                                                                            aaaTCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAAcata                                                                           >  1:1163685/1‑149 (MQ=255)
                                                                              aTCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAAcatcat                                                                         <  2:1159562/150‑1 (MQ=255)
                                                                                 gtcgtTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGCAAACATGCACATCATTGc                                                                      >  1:313400/1‑150 (MQ=255)
                                                                                    gtTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTAGGATTAAGGAAGGATTTTCCTGGAGGAACACATGAACATCATTGCCAt                                                                   >  1:153127/1‑150 (MQ=255)
                                                                                      tATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTa                                                                 <  1:645816/150‑1 (MQ=255)
                                                                                       ttCAGCGCCTGATGAGCTACGGTTACTCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTAt                                                                <  1:630572/149‑1 (MQ=255)
                                                                                        tCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATg                                                               <  2:1024825/150‑1 (MQ=255)
                                                                                        tCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATg                                                               <  2:1879419/150‑1 (MQ=255)
                                                                                        tCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGACCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATg                                                               <  2:105061/150‑1 (MQ=255)
                                                                                          aGCGCCTGATGAGCTACGGTTACGCGAAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTATTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGTAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATggg                                                             >  2:26012/1‑150 (MQ=255)
                                                                                           gcgcCTGCTGAGCTTCGGTTACGCGTAATTCGCAAAAGTTCTGAATAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAAGACATGAACATCATTGCCATTATGGGa                                                            <  1:1404280/150‑1 (MQ=255)
                                                                                              ccTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCg                                                         >  2:913218/1‑150 (MQ=255)
                                                                                                tgatgaGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCa                                                       <  1:2137290/150‑1 (MQ=255)
                                                                                                     aGCTAAGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCg                                                  <  2:180352/150‑1 (MQ=255)
                                                                                                        tACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCt                                               >  2:1724571/1‑150 (MQ=255)
                                                                                                          cGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCATTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCttt                                             <  2:2068587/150‑1 (MQ=255)
                                                                                                          cGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCttt                                             >  2:263733/1‑150 (MQ=255)
                                                                                                          aggTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCATGCTATGATTAAGGAATGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCttt                                             <  2:1050591/149‑1 (MQ=255)
                                                                                                            gTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTta                                           <  1:2183296/150‑1 (MQ=255)
                                                                                                             ttACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTtat                                          <  2:2028178/150‑1 (MQ=255)
                                                                                                             ttACGCGTAATTCCGAAAAGTTCTGAAAAAGGGGCACTTCGGTGGGCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTtat                                          <  2:164999/150‑1 (MQ=255)
                                                                                                                cgcgTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGTTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGTGAACGCATGGCGTCTTTTATaaa                                       >  2:503055/1‑150 (MQ=255)
                                                                                                                    tAATTCGCAAAAGTTCTGTAAAAGGGGCACATCGGTGGCCCTTTTTTAACGCCACCGTTTGAGCAGGGTATGATTAAGGAAGGATTTTCCAGGACGAACACATGAACATCTTTTCCATTATGTGACCGCATGGCGTCTTTTATAAAGATg                                   <  1:345707/150‑1 (MQ=255)
                                                                                                                         ggCAAAAGTTCTGAATACGGGGCACTTCGGGGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAATGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAGccc                              <  2:318703/149‑1 (MQ=255)
                                                                                                                         cGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAGccc                              >  1:1039409/1‑150 (MQ=255)
                                                                                                                         cGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAGccc                              >  2:591776/1‑150 (MQ=255)
                                                                                                                          gctaaaGTCCTGAAAACGCGGCACGTCGGTGTCCAGTTTTTTTAGACACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGCAACACATGAACATCATTGCCATAATGGTACCGCATGGCGTCTTTTATAAAGATGAGCCCa                             <  1:444848/147‑1 (MQ=255)
                                                                                                                          gCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAGCCCa                             >  2:1679655/1‑150 (MQ=255)
                                                                                                                          gCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAGCCCa                             >  1:265779/1‑150 (MQ=255)
                                                                                                                               aGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAGCCCATCaaa                        >  1:1441110/1‑150 (MQ=255)
                                                                                                                                gTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTACGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAGCCCATCAAAg                       <  1:1451132/150‑1 (MQ=255)
                                                                                                                                 cgataaaaaaGGGCCACCGAAGTGACCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAGCCCATCAAAGa                      >  2:1871217/6‑150 (MQ=255)
                                                                                                                                   cTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCTCAACGGTTTGAGCAGGCTATTATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAGCCCATCAAAGAAc                    <  1:2132430/150‑1 (MQ=255)
                                                                                                                                   ataaaaaaGGGCCACCGAAGTGACCCTTTTTTATCGCCATGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAGCCCATCAAAGAAc                    >  2:1511338/4‑150 (MQ=255)
                                                                                                                                     gAAATAGGGTCACTTCGTGGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCGAGGAGGAACACATTAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAGCCCATCAAAGAACTg                  <  1:406173/150‑1 (MQ=255)
                                                                                                                                      aaaaaGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAGCCCATCAAAGAACTgg                 <  2:793244/150‑1 (MQ=255)
                                                                                                                                       aaaaGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAGCCCATCAAAGAACTGGa                >  2:529348/1‑150 (MQ=255)
                                                                                                                                          aGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAGCCCATCAAAGAACTGGAGTc             >  2:180826/1‑150 (MQ=255)
                                                                                                                                          aGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAGCCCATCAAAGAACTGGAGTc             <  2:743689/150‑1 (MQ=255)
                                                                                                                                             gTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAGCCCATCAAAGAACTGGAGTCGgc          >  1:168413/1‑150 (MQ=255)
                                                                                                                                              gcACTTCGCGGGCCCGTTTTTATCGCCACGGTTTGAGCAGGATATGATTAAGGAAGGATTTTCCAGGAGGAACACAGGCCCATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGACCCCATCAAAGAACTGGAGTCGgcg         <  2:302963/149‑1 (MQ=255)
                                                                                                                                               cACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAGCCCATCAAAGAACTGGAGTCGgcgc        <  2:1163685/150‑1 (MQ=255)
                                                                                                                                               cACTTCGGTGGCCCTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAGCCCATCAAAGAACTGGAGTCGGCGCt       <  1:1346272/150‑1 (MQ=255)
                                                                                                                                               cACTTAGGTGGCCCTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCGATTATGGGACCGCATGGCGTGTTTTATAAAGATGAGCCCATCAAAGAACTGGAGTCGGCGCt       <  1:1157012/150‑1 (MQ=255)
                                                                                                                                                aCTTCGGTGGCCCTTTTTAAGCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGCCCGCATGGCGTCTTTTATAAAGATGAGCCCATCAAAGAACTGGAGGCGGCGCt       <  1:412889/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGATGGCGGCATCGTTGAAAGCGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAGCCCATCAAAGAACTGGAGTCGGCGCTGGTGG  >  NC_000913/209462‑209760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: