BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,110,399	G→T	6.0%	noncoding (34/76 nt)	pheV →	tRNA‑Phe

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,110,399	0	G	T	6.0%	476.8 / 14.0	159	noncoding (34/76 nt)	pheV	tRNA‑Phe
Reads supporting (aligned to +/- strand): ref base G (77/72); new base T (3/7); total (80/79)
Fisher's exact test for biased strand distribution p-value = 2.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.42e-01

Only 100 of 164 total aligned reads displayed.

GGTTTGCCTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCAC  >  NC_000913/3110250‑3110548

ggTTTGCCTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTg                                                                                                                                                       >  2:148868/1‑150 (MQ=255)

    tGCCTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGaaaa                                                                                                                                                   >  2:129948/1‑150 (MQ=255)

     gCCTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAAt                                                                                                                                                  >  2:2097671/1‑150 (MQ=255)

      ccTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATc                                                                                                                                                 >  1:854602/1‑150 (MQ=255)

       cTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATcc                                                                                                                                                <  2:95523/150‑1 (MQ=255)

       cTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATcc                                                                                                                                                <  2:802827/150‑1 (MQ=255)

        tGACAATGCGTGCAATATCGGCAAAGTGTTGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCTCCGCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATccc                                                                                                                                               <  1:1616385/150‑1 (MQ=255)

          ccAATGCGTGCAATATCGGCAAAGTGCTGATAGATTGTGCAGTCTGCAGGAAATTGAAGAAATTTGCTTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGAGAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCg                                                                                                                                             <  2:1251493/149‑1 (MQ=255)

          aCAATGCGTGCAATATCGTCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCg                                                                                                                                             <  1:2067622/150‑1 (MQ=255)

           cAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTTTCAATcccctg                                                                                                                                            <  1:1722017/150‑3 (MQ=255)

             aTGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTTTCAATcccctgct                                                                                                                                          <  1:1214889/150‑5 (MQ=255)

             aTGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCgtgt                                                                                                                                          <  1:479637/150‑1 (MQ=255)

              tGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTc                                                                                                                                         >  2:1678517/1‑150 (MQ=255)

                 gTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCtt                                                                                                                                      >  1:990854/1‑150 (MQ=255)

                   gCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTgg                                                                                                                                    <  1:1510644/150‑1 (MQ=255)

                   gCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTgg                                                                                                                                    <  2:1090836/150‑1 (MQ=255)

                      atatCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTc                                                                                                                                 <  2:1519710/150‑1 (MQ=255)

                           ggCAAAGTGATGATAGATGGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTc                                                                                                                            <  1:1693402/150‑1 (MQ=255)

                             cAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCg                                                                                                                          <  2:790285/150‑1 (MQ=255)

                               aaGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAg                                                                                                                        <  1:1183758/150‑1 (MQ=255)

                                     tgatAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGAAAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCggg                                                                                                                  >  2:95063/1‑150 (MQ=255)

                                         aGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGcacc                                                                                                              >  2:1128553/1‑150 (MQ=255)

                                          gATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGcacca                                                                                                             >  2:554254/1‑150 (MQ=255)

                                          gATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGcacca                                                                                                             >  1:1944406/1‑150 (MQ=255)

                                              gtgCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTaa                                                                                                         >  2:439149/1‑150 (MQ=255)

                                               tgCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATTCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGTTTCGATTCCGAGTCCGGGCACCACTAAt                                                                                                        <  2:37252/150‑1 (MQ=255)

                                                  aGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCt                                                                                                     <  1:973541/150‑1 (MQ=255)

                                                   gTCTGCAGTAAATTGAAGAAATATGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCAGGGCACCACTAATTCtt                                                                                                    >  1:1372139/1‑150 (MQ=255)

                                                     cTGCAGTAAATTGACGAACTTTGATTGACGACACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCACAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTGCGATTCCGAGTCCGGGCACCACTAATTCTTaa                                                                                                  <  1:1144670/150‑1 (MQ=21)

                                                     cTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGCTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCATaa                                                                                                  <  2:1126578/150‑1 (MQ=255)

                                                       gCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGa                                                                                                >  1:212862/1‑150 (MQ=255)

                                                          gTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAAcc                                                                                             >  1:641382/1‑150 (MQ=255)

                                                           tAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAAccc                                                                                            >  1:942946/1‑150 (MQ=255)

                                                            aaaTTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAAcccc                                                                                           >  2:2040555/1‑149 (MQ=255)

                                                                tGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCca                                                                                       >  2:1994356/1‑150 (MQ=255)

                                                                tGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCca                                                                                       >  2:625670/1‑150 (MQ=255)

                                                                 gaagaaATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCcac                                                                                      >  2:1843079/1‑150 (MQ=255)

                                                                 gaagaaATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCcac                                                                                      >  2:1091153/1‑150 (MQ=255)

                                                                 gaagaaATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCcac                                                                                      >  1:1667518/1‑150 (MQ=255)

                                                                    gaaATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAg                                                                                   <  1:554254/150‑1 (MQ=255)

                                                                          tgattgaCGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGt                                                                             <  1:595207/150‑1 (MQ=255)

                                                                           gattgaCGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGtt                                                                            >  2:1989918/1‑150 (MQ=255)

                                                                            attgaCGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGttt                                                                           <  2:1499284/150‑1 (MQ=255)

                                                                               gacgagacgaGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTg                                                                        >  2:69983/1‑150 (MQ=255)

                                                                                acgagacgagGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGc                                                                       >  2:973498/1‑150 (MQ=255)

                                                                                 cgagacgagGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCt                                                                      <  1:1448239/150‑1 (MQ=255)

                                                                                   agacgagGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCttt                                                                    <  2:1845580/150‑1 (MQ=255)

                                                                                     ccgagGCGAATCAGGTTTACTGCGCGCCGTTGCCCGGATAGCGCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTg                                                                  <  2:1475206/149‑1 (MQ=255)

                                                                                      cgagGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCAACAAGTCGGGAGTTTGGTTTTgg                                                                 >  2:320020/1‑150 (MQ=18)

                                                                                       gagGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGa                                                                >  1:1893904/1‑150 (MQ=255)

                                                                                         ggCGACTCAGGTTTAATGCGGCCCGGTGCCCGGATACCTCAGTCGGTAGAGCAGGGGATTGACAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATc                                                              <  1:1677209/150‑1 (MQ=21)

                                                                                         ggCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATc                                                              >  2:2072491/1‑150 (MQ=255)

                                                                                         ggCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATc                                                              >  1:577278/1‑150 (MQ=255)

                                                                                         ggCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATc                                                              >  1:1636670/1‑150 (MQ=255)

                                                                                          gCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCt                                                             <  1:2097671/150‑1 (MQ=255)

                                                                                          gCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCt                                                             <  2:864247/150‑1 (MQ=255)

                                                                                           ggAATCAGGTTTAATGCTCCCCGTTGCGCGTATAGCTCAGTCGGTAGAGCAGGGGTTTGAAAATCCCCGTGTCGTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTg                                                            <  2:649725/149‑1 (MQ=21)

                                                                                           cGAATCAGGTTTACTGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCATGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTg                                                            <  1:2124700/150‑1 (MQ=255)

                                                                                             acgcAGGTCTAATGCGCCCCGTTGCCCGGATCGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGATCGATGCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGAc                                                          <  2:1658668/147‑1 (MQ=21)

                                                                                              aTCAGGTTTAATGTGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACa                                                         <  2:6112/150‑1 (MQ=255)

                                                                                                  ggTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCATTTGGATCTGAAAATaa                                                     >  2:914735/1‑150 (MQ=255)

                                                                                                   gTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAAc                                                    >  2:1201829/1‑150 (MQ=255)

                                                                                                      tAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTATATTCCGAGTACGGGCACCACTAAATCTTAACAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCtt                                                 >  1:594294/1‑150 (MQ=21)

                                                                                                         tGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCAc                                              <  1:1128553/150‑1 (MQ=255)

                                                                                                         tGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCAc                                              <  1:439149/150‑1 (MQ=255)

                                                                                                         tGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCAc                                              >  2:1037445/1‑150 (MQ=255)

                                                                                                           cgcCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGa                                            >  1:1621754/1‑150 (MQ=255)

                                                                                                            gcCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGaa                                           >  1:1904327/1‑150 (MQ=255)

                                                                                                              cccGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCAAGaaaa                                         >  1:410616/1‑150 (MQ=255)

                                                                                                              cccGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACACGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGAAAATAACCTTCAAGaaaa                                         >  2:231790/1‑150 (MQ=255)

                                                                                                                   tGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTTATAATCCCCGTATCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGATCCCGCCCACACGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAAAtt                                    <  2:1465104/150‑1 (MQ=25)

                                                                                                                      ccGGATTGATCAGTCGTTAGAGCAGTGGTTTGAAAATCCCCGTGTCCTTGTTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCAACAAGGCGGGTTTTTGCTTTTGGATCTTACAATAACCTTCACGAAAAAAATTAGc                                 <  1:1270708/150‑1 (MQ=37)

                                                                                                                      ccGGATAGCTCAGTCGGTAGAGCAGGGTATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAAATTAGc                                 <  1:1716479/150‑1 (MQ=255)

                                                                                                                        ggATAGGACAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAAATTAGCtt                               <  1:1002225/150‑1 (MQ=255)

                                                                                                                        ggATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAAATTAGCtt                               >  1:456349/1‑150 (MQ=255)

                                                                                                                             gCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTATGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGTTCTGACAATAACCTTCACGAAACAAATTAGCTTATaaa                          >  1:1946270/1‑150 (MQ=255)

                                                                                                                             gCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAAATTAGCTTATaaa                          >  2:1431261/1‑150 (MQ=255)

                                                                                                                             gCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGCTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAAATTAGCTTATaaa                          >  2:127590/1‑150 (MQ=255)

                                                                                                                              cTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAAATTAGCTTATAAAg                         <  1:1843079/150‑1 (MQ=255)

                                                                                                                                    cGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAAATTAGCTTATAAAGTCTggg                   >  1:2059598/1‑150 (MQ=255)

                                                                                                                                     ggTAGAGCAGGTGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAAATTAGCTTATAAAGCCTgggg                  <  2:429598/150‑1 (MQ=255)

                                                                                                                                     ggTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAAATTAGCTTATAAAGTCTgggg                  <  1:1033070/150‑1 (MQ=255)

                                                                                                                                      gTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAAATTAGCTTATAAAGTCTggggg                 <  1:1349475/150‑1 (MQ=255)

                                                                                                                                         gagCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAAATTAGCTTATAAAGTCTGGGGGAAt              >  1:908109/1‑150 (MQ=255)

                                                                                                                                         gagCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAAATTAGCTTATAAAGTCTGGGGGAAt              <  2:1904327/150‑1 (MQ=255)

                                                                                                                                         gacacggggATTTTCAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAAATTAGCTTATAAAGTCTGGGGGAAt              >  2:683299/6‑150 (MQ=255)

                                                                                                                                          acacggggATTTTCAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAAATTAGCTTATAAAGTCTGGGGGAAtt             >  2:2155430/5‑150 (MQ=255)

                                                                                                                                           gCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAAATTAGCTTATAAAGTCTGGGGGAATTa            <  2:1944406/150‑1 (MQ=255)

                                                                                                                                           cacggggATTTTCAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAAATTAGCTTATAAAGTCTGGGGGAATTa            >  2:2059885/4‑150 (MQ=255)

                                                                                                                                              ggggATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCCCCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGATAAAAATTAGCTTATAAAGTCTGGGGGAATTActg         >  2:386181/1‑149 (MQ=255)

                                                                                                                                              ggggATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAAATTAGCTTATAAAGTCTGGGGGAATTActc         >  1:720445/1‑150 (MQ=255)

                                                                                                                                              ggggATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAAATTAGCTTATAAAGTCTGGGGGAATTActc         <  2:1222509/150‑1 (MQ=255)

                                                                                                                                               gggATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAAATTAGCTTATAAAGTCTGGGGGAATTActct        >  1:1852519/1‑150 (MQ=255)

                                                                                                                                                ggATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAAATTAGCTTATAAAGTCTGGGGGAATTACtctc       >  2:2061586/1‑150 (MQ=255)

                                                                                                                                                 gATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCTCAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCg      >  2:1148192/1‑150 (MQ=255)

                                                                                                                                                  aTTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGc     >  1:684555/1‑150 (MQ=255)

                                                                                                                                                   ttGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCCCCACTAATTCTTAAGAACCCGCCCACAAGGCGGGGTTTTGCTTTTGGAGCTGACAATAACCTTCACGAAAAAAATTAGCTTATAAAGTCTGGGTGAATTACTCTCgac    >  1:1129739/1‑148 (MQ=255)

                                                                                                                                                    tGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGGCCGTGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCa   <  1:148868/150‑1 (MQ=255)

                                                                                                                                                     gAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCAc  >  2:560201/1‑150 (MQ=255)

                                                                                                                                                     gAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCAc  >  1:906507/1‑150 (MQ=255)

GGTTTGCCTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAACCTTCACGAAAAAAATTAGCTTATAAAGTCTGGGGGAATTACTCTCGCCAC  >  NC_000913/3110250‑3110548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`