Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 209,615 G→A 6.9% intergenic (+35/‑64) accA → / → ldcC acetyl‑CoA carboxylase, carboxytransferase, alpha subunit/lysine decarboxylase 2, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913209,6150GA 6.9% 360.8 / 9.7 117intergenic (+35/‑64)accA/ldcCacetyl‑CoA carboxylase, carboxytransferase, alpha subunit/lysine decarboxylase 2, constitutive
Reads supporting (aligned to +/- strand):  ref base G (47/60);  new base A (4/4);  total (52/65)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 119 total aligned reads displayed.

GGCGGCATCGTTGAAAGCGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAGCCCATCAAAGAACTGGAGTCGGCGCTGGTGGC  >  NC_000913/209466‑209761
                                                                                                                                                     |                                                                                                                                                  
ggcggcATCGTTGAAAGCGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTgg                                                                                                                                                    <  1:1735201/150‑1 (MQ=255)
ggcggcATCGTTGAAAGCGAAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTGCTGAAAAAGGGTCACTTCGGTgg                                                                                                                                                    <  2:514593/150‑1 (MQ=255)
 gcggcATCGTTGAAAGCGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGc                                                                                                                                                   >  1:1549006/1‑150 (MQ=255)
     cATCGTTGAAAGCGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCtt                                                                                                                                               >  2:761866/1‑150 (MQ=255)
       tCGTTGAAAGCGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCtttt                                                                                                                                             <  2:138978/150‑1 (MQ=255)
         gTTGAAAGCGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCtttttt                                                                                                                                           >  2:1930770/1‑150 (MQ=255)
          ttGAAAGCGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGCCACCGAAGTGACCCtttttca                                                                                                                                          <  1:535184/150‑3 (MQ=255)
           tGAAAGCGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGCCACCGAAGTGACCCtttttcag                                                                                                                                         <  1:1694727/150‑4 (MQ=255)
            gAAAGCGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCATCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATc                                                                                                                                        >  2:345707/1‑150 (MQ=255)
              tagagcAACTGGTGGCGGTTCTGGCAGATGACGACGTGTTAAGCACTGATTATTTAAAATATCGTAGTTTTCAGCGCCTGATGAGCTACGGTAACGCGCAATTAGCAATAGTTCTGAAAAAGGGTCACTTAGGTTGCCCTTTTTTATCGc                                                                                                                                      <  2:408722/146‑1 (MQ=255)
                    aaCTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGt                                                                                                                                <  2:706050/150‑1 (MQ=255)
                      ctgctgGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGttt                                                                                                                              <  2:892863/150‑1 (MQ=255)
                       tgctgGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGTGCTACGGATACGCGTAATTCGCAAAAGTTCTGAAAAAGGGGCACTTCGGTGGCCCTTTTTTATCGCCTCGGTTTg                                                                                                                             <  1:1863422/150‑1 (MQ=255)
                        gctgGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGa                                                                                                                            <  2:1456216/150‑1 (MQ=255)
                        gctgGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGa                                                                                                                            >  2:406173/1‑150 (MQ=255)
                         cttccGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCTTAATTCGCAAAAGTTCTGAAAAAGTGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAg                                                                                                                           <  1:1677109/146‑1 (MQ=255)
                         ctgGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCGTTTTTATCGCCACGGTTTGAg                                                                                                                           >  1:1396700/1‑150 (MQ=255)
                           ggCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGACCTTTTTTATCGCGGCGGTTTGAGCa                                                                                                                         >  2:313528/1‑150 (MQ=255)
                             cGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTTCGCCACGGTTTGAGCAgga                                                                                                                      >  2:1157012/1‑149 (MQ=255)
                              ggATCTGGCCGATCTCGACGTGTTAAGCAATGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGc                                                                                                                      <  2:1217764/150‑1 (MQ=255)
                                aTCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTa                                                                                                                    <  1:391238/150‑1 (MQ=255)
                                aTCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTa                                                                                                                    <  1:2041994/150‑1 (MQ=255)
                                 tCTGGCCGTTCTCGACGTGTTCAGCCCTGACGAATTAAAAAATCGTCGTTATCAGAGCCTGATGAGCTACGGGTACGCGTAATTCGAAAAAGTTCTGAAAAAGGGTCACATCGGTGGCCCTTTTTTATCTCCACGGTTTGAGCAGGCTAt                                                                                                                   <  1:1155582/150‑1 (MQ=255)
                                  cTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATg                                                                                                                  <  2:1166583/150‑1 (MQ=255)
                                  cTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATg                                                                                                                  >  2:2046818/1‑150 (MQ=255)
                                   tGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAATGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGa                                                                                                                 <  1:1268792/150‑1 (MQ=255)
                                    ggCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGAt                                                                                                                >  1:609035/1‑150 (MQ=255)
                                      ccGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTa                                                                                                              >  2:645816/1‑150 (MQ=255)
                                      ccGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTa                                                                                                              >  2:1854195/1‑150 (MQ=255)
                                        gATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTaag                                                                                                            <  2:1491260/150‑1 (MQ=255)
                                        gATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTaag                                                                                                            <  2:1314215/150‑1 (MQ=255)
                                          tctcGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTaagga                                                                                                          <  2:1047444/150‑1 (MQ=255)
                                            tcGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTaaggaag                                                                                                        >  1:2149792/1‑150 (MQ=255)
                                               aCGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGAt                                                                                                     <  2:1977886/150‑1 (MQ=255)
                                                   gtTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGAGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTATTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTc                                                                                                 >  1:1575573/1‑150 (MQ=255)
                                                      aaGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCTAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTGTGAGCATGCTATGATTAAGGAAGGATTTTCCag                                                                                              <  1:1525719/150‑1 (MQ=255)
                                                      aaGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCag                                                                                              >  1:1229820/1‑150 (MQ=255)
                                                         cACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGTAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCaggag                                                                                           <  2:1789655/150‑1 (MQ=255)
                                                         cACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCaggag                                                                                           <  2:246176/150‑1 (MQ=255)
                                                         cACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCaggag                                                                                           <  1:1767783/150‑1 (MQ=255)
                                                           cTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCCCGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAggagga                                                                                         >  2:1185723/1‑150 (MQ=255)
                                                           cTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGAAAAAGTTCTGAAAAAGGGTCACTTCGTTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTACAggagga                                                                                         <  1:1562812/150‑1 (MQ=255)
                                                           cTGAAGATTTAAAAAATCGTCGTTATCAGCGCCCGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAGGATTTTCCAGGAGGaa                                                                                        >  1:828423/1‑150 (MQ=255)
                                                             gAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAac                                                                                       >  1:2124006/1‑150 (MQ=255)
                                                             gAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGCCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCAAGGAGGAac                                                                                       >  2:1226681/1‑150 (MQ=255)
                                                              aaGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAaca                                                                                      <  2:1549006/150‑1 (MQ=255)
                                                               aGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAacac                                                                                     <  1:2205712/150‑1 (MQ=255)
                                                                gATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAAcaca                                                                                    >  2:1770965/1‑150 (MQ=255)
                                                                  tttAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCGTTTTTATCGCCACGGTTTGAGCAGGCTATGAGTAAGGAAGGATTTTCCAGGAGGAACACATg                                                                                  >  2:1065575/1‑150 (MQ=255)
                                                                    tAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGaa                                                                                >  1:1811259/1‑150 (MQ=255)
                                                                     aaaaaaTCGTCGGTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAAc                                                                               <  2:1205146/150‑1 (MQ=255)
                                                                       aaaaTCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAAcat                                                                             >  2:634804/1‑150 (MQ=255)
                                                                        aaaTCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAAcatc                                                                            >  1:489474/1‑150 (MQ=255)
                                                                        aaaTCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAAcata                                                                            >  1:1163685/1‑149 (MQ=255)
                                                                          aTCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAAcatcat                                                                          <  2:1159562/150‑1 (MQ=255)
                                                                             gtcgtTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGCAAACATGCACATCATTGc                                                                       >  1:313400/1‑150 (MQ=255)
                                                                                gtTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTAGGATTAAGGAAGGATTTTCCTGGAGGAACACATGAACATCATTGCCAt                                                                    >  1:153127/1‑150 (MQ=255)
                                                                                  tATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTa                                                                  <  1:645816/150‑1 (MQ=255)
                                                                                   ttCAGCGCCTGATGAGCTACGGTTACTCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTAt                                                                 <  1:630572/149‑1 (MQ=255)
                                                                                    tCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATg                                                                <  2:1024825/150‑1 (MQ=255)
                                                                                    tCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGACCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATg                                                                <  2:105061/150‑1 (MQ=255)
                                                                                      aGCGCCTGATGAGCTACGGTTACGCGAAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTATTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGTAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATggg                                                              >  2:26012/1‑150 (MQ=255)
                                                                                       gcgcCTGCTGAGCTTCGGTTACGCGTAATTCGCAAAAGTTCTGAATAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAAGACATGAACATCATTGCCATTATGGGa                                                             <  1:1404280/150‑1 (MQ=255)
                                                                                          ccTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCg                                                          >  2:913218/1‑150 (MQ=255)
                                                                                           cTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTAGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGc                                                         <  2:1613776/150‑1 (MQ=255)
                                                                                            tgatgaGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCa                                                        <  1:2137290/150‑1 (MQ=255)
                                                                                                 aGCTAAGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCg                                                   <  2:180352/150‑1 (MQ=255)
                                                                                                    tACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCt                                                >  2:1724571/1‑150 (MQ=255)
                                                                                                      cGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCATTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCttt                                              <  2:2068587/150‑1 (MQ=255)
                                                                                                      cGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCttt                                              >  2:263733/1‑150 (MQ=255)
                                                                                                      aggTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCATGCTATGATTAAGGAATGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCttt                                              <  2:1050591/149‑1 (MQ=255)
                                                                                                        gTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTta                                            <  1:2183296/150‑1 (MQ=255)
                                                                                                         ttACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTtat                                           <  2:2028178/150‑1 (MQ=255)
                                                                                                         ttACGCGTAATTCCGAAAAGTTCTGAAAAAGGGGCACTTCGGTGGGCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTtat                                           <  2:164999/150‑1 (MQ=255)
                                                                                                            cgcgTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGTTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGTGAACGCATGGCGTCTTTTATaaa                                        >  2:503055/1‑150 (MQ=255)
                                                                                                                tAATTCGCAAAAGTTCTGTAAAAGGGGCACATCGGTGGCCCTTTTTTAACGCCACCGTTTGAGCAGGGTATGATTAAGGAAGGATTTTCCAGGACGAACACATGAACATCTTTTCCATTATGTGACCGCATGGCGTCTTTTATAAAGATg                                    <  1:345707/150‑1 (MQ=255)
                                                                                                                     ggCAAAAGTTCTGAATACGGGGCACTTCGGGGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAATGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAGccc                               <  2:318703/149‑1 (MQ=255)
                                                                                                                     cGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAGccc                               >  1:1039409/1‑150 (MQ=255)
                                                                                                                     cGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAGccc                               >  2:591776/1‑150 (MQ=255)
                                                                                                                      gctaaaGTCCTGAAAACGCGGCACGTCGGTGTCCAGTTTTTTTAGACACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGCAACACATGAACATCATTGCCATAATGGTACCGCATGGCGTCTTTTATAAAGATGAGCCCa                              <  1:444848/147‑1 (MQ=255)
                                                                                                                      gCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAGCCCa                              >  2:1679655/1‑150 (MQ=255)
                                                                                                                      gCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAGCCCa                              >  1:265779/1‑150 (MQ=255)
                                                                                                                           aGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAGCCCATCaaa                         >  1:1441110/1‑150 (MQ=255)
                                                                                                                            gTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTACGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAGCCCATCAAAg                        <  1:1451132/150‑1 (MQ=255)
                                                                                                                             cgataaaaaaGGGCCACCGAAGTGACCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAGCCCATCAAAGa                       >  2:1871217/6‑150 (MQ=255)
                                                                                                                               cTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCTCAACGGTTTGAGCAGGCTATTATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAGCCCATCAAAGAAc                     <  1:2132430/150‑1 (MQ=255)
                                                                                                                               ataaaaaaGGGCCACCGAAGTGACCCTTTTTTATCGCCCCGGTTTGAGCAGGCTATGAATAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGCGACCGCATGGCGTCTTTTATAAAGATGAGCCCATCAaacaac                     >  2:1381635/4‑150 (MQ=255)
                                                                                                                               ataaaaaaGGGCCACCGAAGTGACCCTTTTTTATCGCCATGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAGCCCATCAAAGAAc                     >  2:1511338/4‑150 (MQ=255)
                                                                                                                                 gAAATAGGGTCACTTCGTGGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCGAGGAGGAACACATTAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAGCCCATCAAAGAACTg                   <  1:406173/150‑1 (MQ=255)
                                                                                                                                  aaaaaGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAGCCCATCAAAGAACTgg                  <  2:793244/150‑1 (MQ=255)
                                                                                                                                   aaaaGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAGCCCATCAAAGAACTGGa                 >  2:529348/1‑150 (MQ=255)
                                                                                                                                      aGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAGCCCATCAAAGAACTGGAGTc              >  2:180826/1‑150 (MQ=255)
                                                                                                                                      aGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAGCCCATCAAAGAACTGGAGTc              <  2:743689/150‑1 (MQ=255)
                                                                                                                                         gTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAGCCCATCAAAGAACTGGAGTCGgc           >  1:168413/1‑150 (MQ=255)
                                                                                                                                          gcACTTCGCGGGCCCGTTTTTATCGCCACGGTTTGAGCAGGATATGATTAAGGAAGGATTTTCCAGGAGGAACACAGGCCCATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGACCCCATCAAAGAACTGGAGTCGgcg          <  2:302963/149‑1 (MQ=255)
                                                                                                                                           cACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAGCCCATCAAAGAACTGGAGTCGgcgc         <  2:1163685/150‑1 (MQ=255)
                                                                                                                                           cACTTCGGTGGCCCTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAGCCCATCAAAGAACTGGAGTCGGCGCt        <  1:1346272/150‑1 (MQ=255)
                                                                                                                                           cACTTAGGTGGCCCTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCGATTATGGGACCGCATGGCGTGTTTTATAAAGATGAGCCCATCAAAGAACTGGAGTCGGCGCt        <  1:1157012/150‑1 (MQ=255)
                                                                                                                                            aCTTCGGTGGCCCTTTTTAAGCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGCCCGCATGGCGTCTTTTATAAAGATGAGCCCATCAAAGAACTGGAGGCGGCGCt        <  1:412889/150‑1 (MQ=255)
                                                                                                                                                  gTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAGCCCATCAAAGAACTGGAGTCGGCGCTGGTGgc  <  2:1229820/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
GGCGGCATCGTTGAAAGCGCAACTGCTGGCGGATCTGGCCGATCTCGACGTGTTAAGCACTGAAGATTTAAAAAATCGTCGTTATCAGCGCCTGATGAGCTACGGTTACGCGTAATTCGCAAAAGTTCTGAAAAAGGGTCACTTCGGTGGCCCTTTTTTATCGCCACGGTTTGAGCAGGCTATGATTAAGGAAGGATTTTCCAGGAGGAACACATGAACATCATTGCCATTATGGGACCGCATGGCGTCTTTTATAAAGATGAGCCCATCAAAGAACTGGAGTCGGCGCTGGTGGC  >  NC_000913/209466‑209761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: