Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,448,278 C→A 5.3% intergenic (‑129/+36) rplN ← / ← rpsQ 50S ribosomal subunit protein L14/30S ribosomal subunit protein S17

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,448,2780CA 5.3% 431.5 / 9.9 145intergenic (‑129/+36)rplN/rpsQ50S ribosomal subunit protein L14/30S ribosomal subunit protein S17
Reads supporting (aligned to +/- strand):  ref base C (73/62);  new base A (3/5);  total (77/68)
Fisher's exact test for biased strand distribution p-value = 4.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.25e-01

Only 100 of 147 total aligned reads displayed.

CATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCACGTCACCGATACCGCATTCGTTGTTCTCGTC  >  NC_000913/3448129‑3448427
                                                                                                                                                     |                                                                                                                                                     
cATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTc                                                                                                                                                       <  2:941905/150‑1 (MQ=255)
 aTAGTCTGTTATTGGATCATTTTAGTGCTCCGCTAATTTAAACTAGTACTTTGTCCCGAAAAGCAGGTCGTTTAAAATCCCCATCTCGAGGGCGCGGCATTATAAGACCGCTTCAAGGTTATGGGTAGAAAAACTAAACGGATCAATTCt                                                                                                                                                      <  2:1058026/150‑1 (MQ=255)
     tCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGc                                                                                                                                                  <  2:3607/150‑1 (MQ=255)
      cTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGcc                                                                                                                                                 >  2:862178/1‑150 (MQ=255)
       tGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCg                                                                                                                                                <  2:712031/150‑1 (MQ=255)
        gTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGt                                                                                                                                               >  1:1201597/1‑150 (MQ=255)
         ttCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCAGAAATGAGCCGtt                                                                                                                                              <  2:1284693/150‑1 (MQ=255)
          tCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATTACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGttt                                                                                                                                             >  1:1504470/1‑150 (MQ=255)
          tCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCAGAAATGAGCCGttt                                                                                                                                             <  2:2099026/150‑1 (MQ=255)
          tCTTGGATCATTTTAGTGCTACGCTATTGTCAACTAATACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTGCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGttt                                                                                                                                             <  1:115246/150‑1 (MQ=255)
            ttGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCTAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCAGAAATGAGCCGTTTAt                                                                                                                                           <  1:776615/150‑1 (MQ=255)
             tGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTAtt                                                                                                                                          >  1:1395806/1‑150 (MQ=255)
              ggATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTc                                                                                                                                         >  1:59094/1‑150 (MQ=255)
                  cATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCTTAt                                                                                                                                     >  1:163820/1‑150 (MQ=255)
                  cATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTAt                                                                                                                                     >  1:453076/1‑150 (MQ=255)
                   catttAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTAtt                                                                                                                                    <  2:1407898/148‑1 (MQ=255)
                   aTTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTAtt                                                                                                                                    <  1:511272/150‑1 (MQ=255)
                       tAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTgaga                                                                                                                                >  2:661619/1‑150 (MQ=255)
                          tGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGagagag                                                                                                                             >  2:2172251/1‑150 (MQ=255)
                            cTCCGCTAATGTCAACTACTACTGAGACACGAAAATCAGGTCGTTAAAAATCCCCATCTCGAGGGCGCGGCATTATAACCCCGCTTCACGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAgtg                                                                                                                           <  1:2047356/150‑1 (MQ=255)
                             tCCTCTAATGTCAACTACTACTTATACCCTAAAATCAGGTGGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAgtgt                                                                                                                          <  1:210240/150‑1 (MQ=255)
                             tCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAgtgt                                                                                                                          >  2:1308142/1‑150 (MQ=255)
                                gCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACt                                                                                                                       <  1:30031/150‑1 (MQ=255)
                                  tAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGt                                                                                                                     <  2:608003/150‑1 (MQ=255)
                                  tAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGt                                                                                                                     >  2:1225018/1‑150 (MQ=255)
                                  tAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGt                                                                                                                     <  1:1149138/150‑1 (MQ=255)
                                  tAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGCGCGCTGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGTCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGt                                                                                                                     >  1:1564036/1‑150 (MQ=255)
                                  tAATGTCAACTACTACTGAGAACCGAAAATCATGTCGTTAAAAATCCCCATATAGAGGTCGCGGCATTATAACACCGATTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGt                                                                                                                     <  1:59051/150‑1 (MQ=255)
                                  aaaTTTCAACTACTACTCAGACCCCAAAATCAGGTCGTTAAATATCCCCATATCGATGGCGCGGCATTATAACACCGCTTAAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGt                                                                                                                     <  2:2208956/149‑1 (MQ=255)
                                      gTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTAGTATTGAGAGAGTGTACTGTATTa                                                                                                                 >  2:2022452/1‑150 (MQ=255)
                                       tCAAATACTACTGATATCCGAAAATCAGGTCGATAAAAATGCTCATATCGAGGGCGCGGAATTATACCACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTAc                                                                                                                <  1:1927468/150‑1 (MQ=255)
                                        cAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGTGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTAATTTCTGAGCCGTTTATTCGTATTTAGAGATTGTACTGTATTACa                                                                                                               >  1:376032/1‑150 (MQ=255)
                                        cAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACa                                                                                                               >  2:1825993/1‑150 (MQ=255)
                                        cAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACa                                                                                                               <  2:2171135/150‑1 (MQ=255)
                                         tactactactGAGTCCAGAAAATCAGGTCGTTAAAAATCCCCATATCGATTGCGCGGCATTTTAACAGCGCTTCAAGGTTATGGGTAGAAAAAATAAACGGCTCATTTGTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAg                                                                                                              <  1:91228/149‑1 (MQ=255)
                                          actactactGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTATAAAAAATAAACGCCTCATTTCTGAGCCGTTTATTCGTATTGAGAGATTGTACTTTATTACAGa                                                                                                             >  2:1211867/1‑150 (MQ=255)
                                              ctactGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCg                                                                                                         <  1:817744/150‑1 (MQ=255)
                                                 ctGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACCCCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTTTTGAGAGAGTGTACTGTATTACAGAACCGCtt                                                                                                      >  2:897422/1‑150 (MQ=255)
                                                  tGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCttt                                                                                                     >  1:243824/1‑150 (MQ=255)
                                                  tGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAATGTACTGTATTACAGAACCGCttt                                                                                                     >  2:1863460/1‑150 (MQ=255)
                                                    agaCCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTtct                                                                                                   <  1:1294556/150‑1 (MQ=255)
                                                       cccGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTa                                                                                                >  2:17506/1‑150 (MQ=255)
                                                       cccGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTa                                                                                                >  2:1070871/1‑150 (MQ=255)
                                                       cccGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTa                                                                                                >  2:402417/1‑150 (MQ=255)
                                                        ccGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTAc                                                                                               >  2:958507/1‑150 (MQ=255)
                                                        ccGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTAc                                                                                               >  1:2187169/1‑150 (MQ=255)
                                                         cGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACa                                                                                              >  1:32520/1‑150 (MQ=255)
                                                         cGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGAATTACAGAACCGCTTTCTCTACa                                                                                              >  2:1059077/1‑150 (MQ=255)
                                                          gAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACaa                                                                                             >  1:1912184/1‑150 (MQ=255)
                                                             aaTCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAAcgc                                                                                          <  2:415776/150‑1 (MQ=255)
                                                                  ggTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACACCGCGAAcc                                                                                     >  1:222910/1‑150 (MQ=255)
                                                                  ggTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAAcc                                                                                     <  2:793321/150‑1 (MQ=255)
                                                                     cGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGc                                                                                  >  2:668035/1‑150 (MQ=255)
                                                                      gTTAAAAATCCCCATATCGCGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCTTTTATTCGTATTGAGAGAGTGTACTGTATTACAGCACCGCTTTCTCTACAACGCGAACCAGCg                                                                                 >  2:1308893/1‑150 (MQ=255)
                                                                       ttAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGt                                                                                >  2:1037488/1‑150 (MQ=255)
                                                                         aaaaaTCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTcc                                                                              <  1:1277364/150‑1 (MQ=255)
                                                                           aaaTCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAg                                                                            <  1:1239972/150‑1 (MQ=255)
                                                                                  cATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAATGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAg                                                                     <  1:132723/150‑1 (MQ=255)
                                                                                  cATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAg                                                                     >  1:2043326/1‑150 (MQ=255)
                                                                                  cATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGCGTAGAAAAAATAAACGGCTCATTTCTGCGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAg                                                                     <  2:1435814/150‑1 (MQ=255)
                                                                                      tCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCtt                                                                 <  2:76348/150‑1 (MQ=255)
                                                                                        gAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGGTTATTCGTATTGAGAGAGTGTACTGTTTTACAGAACGGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTgg                                                               >  2:178304/1‑150 (MQ=255)
                                                                                        gAGGGCGCGGCATTAAAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTgg                                                               <  2:666545/150‑1 (MQ=255)
                                                                                                ggCATTATAACACCGCTTCAAGTATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAAGACTAAATCCTGGAcgctggt                                                       <  2:1556829/150‑7 (MQ=255)
                                                                                                 gCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTGTCTCTACAAAGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGAc                                                      >  2:1251953/1‑150 (MQ=255)
                                                                                                   aTTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACgg                                                    <  1:1092747/150‑1 (MQ=255)
                                                                                                   aTTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTAATGAGAGAGTGTACTGTATTACAGAAACGCTTTCTCTACAACGCCAACCAGCGTCCAGGAATTAGTCTTGTACAGCGTACgg                                                    >  1:841868/1‑150 (MQ=255)
                                                                                                    ttATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGc                                                   <  2:1757994/150‑1 (MQ=255)
                                                                                                       taACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCAtt                                                <  1:72874/150‑1 (MQ=255)
                                                                                                        aaCACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTc                                               >  2:1547469/1‑150 (MQ=255)
                                                                                                         acacCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCTTAATGAGAAAGTGAAATGTAATACATAACAGCATTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTcg                                              >  2:498325/1‑150 (MQ=255)
                                                                                                         acacCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTcg                                              >  2:684481/1‑150 (MQ=255)
                                                                                                           acCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGCGCGAGTGTCCTGTATTACAGAACCGCGTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTcgcg                                            <  1:1439515/150‑1 (MQ=255)
                                                                                                             cGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGGCAGCGGACGGCATTCGCGGa                                          <  1:395608/150‑1 (MQ=255)
                                                                                                              gCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCATGGACAGCGGACGGCATTCGCGGAt                                         >  2:148122/1‑150 (MQ=255)
                                                                                                               cTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGAGAACAAGCGTCCAGGATTTAGTAATGAACAGCGGACGGCATTCGCGGAtt                                        >  1:1495736/1‑150 (MQ=255)
                                                                                                                ttCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGAttt                                       <  1:209597/150‑1 (MQ=255)
                                                                                                                 tCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGAttta                                      >  1:1680847/1‑149 (MQ=255)
                                                                                                                 tCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTc                                      >  1:1285038/1‑150 (MQ=255)
                                                                                                                  aaaGGATATTGTTAGAAAAAATAAACGGCTCATTTCTGATCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGATTTCTCTACAACTCTAAACAGCGTCCAGTATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCa                                     <  2:376032/149‑1 (MQ=255)
                                                                                                                          tGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACAGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCACGTc                             >  2:427967/1‑150 (MQ=255)
                                                                                                                           gggTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCACGTCa                            <  1:2172251/150‑1 (MQ=255)
                                                                                                                           gggTAGAAAAAATAAACGGCTAATTTCTGAGCCTTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAAAGCGAACCAGCGTCCAGGATTTAGACTAGGACAGCGGTAGGAATTCGCGGATTTCAACCACGTCa                            >  2:1742870/1‑150 (MQ=255)
                                                                                                                            ggTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCACGTCAc                           >  2:2020328/1‑150 (MQ=255)
                                                                                                                             gTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCACGTCAcc                          >  2:2135474/1‑150 (MQ=255)
                                                                                                                              tataaaaaaTAAACGTCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCACGTCACCg                         <  1:888783/147‑1 (MQ=255)
                                                                                                                              caatacgaaTAAACGGCTCAGAAATGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCACCCACGTCACCg                         >  2:47556/8‑150 (MQ=38)
                                                                                                                               aGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTAGTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCACGTCACCGa                        >  1:956789/1‑150 (MQ=255)
                                                                                                                                gAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCACGTCACCGAt                       >  2:1682743/1‑150 (MQ=255)
                                                                                                                                 aaaaaaTAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTAATGTATTACAGATCCGCTGTCTCTACAACGCGAAACAGCGTCCAGGATTTAGTCTTTGACAGCGGACGGCATTAGCGGATTTAAACCACGTCACCGATa                      >  1:375129/1‑150 (MQ=255)
                                                                                                                                  aaaaaTAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCCGAGTCCAGGATTTAGTATTGGACAGCGGACGGCATTCGCGGATTTCACCCACGGCACCGATAc                     >  2:1326447/1‑150 (MQ=255)
                                                                                                                                      aTAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCACGTCACCGATACCGCa                 >  1:1136911/1‑150 (MQ=255)
                                                                                                                                       tAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCACCCACGTCACCGATACCGCAt                >  1:88005/1‑150 (MQ=255)
                                                                                                                                          aCGGGTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGACCCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTGTTGTACAGCGGACGGCATTCGCGGATTTCAACCACGTCACCGATACCGCAttag             <  2:148588/150‑3 (MQ=255)
                                                                                                                                            ggCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCACGTCACCGATACCGCATTCgtt           >  1:1075933/1‑150 (MQ=255)
                                                                                                                                              cTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCACGTCACCGATACCGCATTCgttgt         <  2:243824/150‑1 (MQ=255)
                                                                                                                                              atCATTTCTGAGCCGTTTATTCGTATTGAGAGATTGTACTGTATTACAGAACCGCTATCTCTACAACTCGAAGCAGCGTCCAGGATTTAGTCTTGGAAAGCGGACGGCATTCGCGGATTTCAACCACGTCACCGATACCGCATTCgtttt         <  2:1363522/149‑3 (MQ=255)
                                                                                                                                                cATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCACGTCACCGATACCGCATTCGTTGTtc       <  1:1341928/150‑1 (MQ=255)
                                                                                                                                                    tCTGAGCCGTTTATTCGTATTGAGAGAGTGGACTGTATTACTGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGTTTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCACGTCACCGATACCGCATTCGTTGTTCTCGt   <  1:1211867/150‑1 (MQ=255)
                                                                                                                                                     cTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCACGTCCCCGCTACCGCATTCGTCGTTCTCGTc  >  2:1004721/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CATAGTCTGTTCTTGGATCATTTTAGTGCTCCGCTAATGTCAACTACTACTGAGACCCGAAAATCAGGTCGTTAAAAATCCCCATATCGAGGGCGCGGCATTATAACACCGCTTCAAGGATATGGGTAGAAAAAATAAACGGCTCATTTCTGAGCCGTTTATTCGTATTGAGAGAGTGTACTGTATTACAGAACCGCTTTCTCTACAACGCGAACCAGCGTCCAGGATTTAGTCTTGGACAGCGGACGGCATTCGCGGATTTCAACCACGTCACCGATACCGCATTCGTTGTTCTCGTC  >  NC_000913/3448129‑3448427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: