Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,510,357 G→T 5.9% P207Q (CCG→CAG)  yhfX ← putative pyridoxal 5'‑phosphate binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,510,3570GT 5.9% 338.0 / 4.7 127P207Q (CCG→CAG) yhfXputative pyridoxal 5'‑phosphate binding protein
Reads supporting (aligned to +/- strand):  ref base G (54/64);  new base T (7/2);  total (61/66)
Fisher's exact test for biased strand distribution p-value = 8.66e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 130 total aligned reads displayed.

GACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCGGCAACACCTTGAGGGCAAAACCGCTCTCCTGGCCCGGATAAAGAAAA  >  NC_000913/3510208‑3510506
                                                                                                                                                     |                                                                                                                                                     
gACCGGGTTCTGCATGAGTCACACCGTTTTGCGCCAGTAATGGCTGCGAAGTGCAGCTGGTCGCGGCAGGCGCGTTAAGTTGCGCAAGTGCAATACCAGATTTATACAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCg                                                                                                                                                       <  2:357221/150‑1 (MQ=255)
 aCCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCgg                                                                                                                                                      >  1:538093/1‑150 (MQ=255)
    gggTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCaa                                                                                                                                                   >  1:2152019/1‑150 (MQ=255)
      gTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCCGCGTGTGAAGATTCGGTGTAGGCaaaa                                                                                                                                                 >  1:665060/1‑150 (MQ=255)
        tCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACt                                                                                                                                               >  1:132746/1‑150 (MQ=255)
           gCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACtttt                                                                                                                                            >  1:1676520/1‑150 (MQ=255)
           gCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATACCGTTTCTACaaaaataat                                                                                                                                            >  2:644625/1‑145 (MQ=255)
                 gTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACa                                                                                                                                      >  2:860341/1‑150 (MQ=255)
                   cacaCCTTATTTCGCCAGTAATTGCAGCGAAGTGCAGCTGGTCGCTGAAGGGGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCATCGTGTGAAGATTCGGTGTCGGAAAAGTTTTGCCGACacc                                                                                                                                    <  1:14606/150‑3 (MQ=255)
                    acacCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTTTCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGcc                                                                                                                                   >  1:872543/1‑150 (MQ=255)
                    acacCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGcc                                                                                                                                   <  2:183579/150‑1 (MQ=255)
                     cacCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCt                                                                                                                                  <  1:1485229/150‑1 (MQ=255)
                     cacCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTAGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCt                                                                                                                                  >  2:585935/1‑150 (MQ=255)
                     cacCGTATTGCGACAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGAACCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCt                                                                                                                                  >  1:847427/1‑150 (MQ=255)
                      acCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGAAAAGTTTTGCCGACaccgaa                                                                                                                                 <  2:1647276/150‑6 (MQ=255)
                         gTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATc                                                                                                                              >  1:2137328/1‑150 (MQ=255)
                         gTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATc                                                                                                                              <  2:1324456/150‑1 (MQ=255)
                          tATTGCTCCAGTATTGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGGTCAAGTGCAATACCAGATTTCTCAAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATcc                                                                                                                             <  2:743815/150‑1 (MQ=255)
                              gcgcCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCaaa                                                                                                                         >  1:1719296/1‑150 (MQ=255)
                                gcCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGc                                                                                                                       >  1:1055366/1‑150 (MQ=255)
                                   aGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAgg                                                                                                                    <  2:455183/150‑1 (MQ=255)
                                       aTGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGTTCCGGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACGTTTCAGACAGCCTCATCCCAAAGGAGGCAAg                                                                                                                >  1:331909/1‑150 (MQ=255)
                                       aTGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAg                                                                                                                <  2:2007900/150‑1 (MQ=255)
                                        tGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAgg                                                                                                               <  1:1376787/150‑1 (MQ=255)
                                         ggCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAggg                                                                                                              <  2:703445/150‑1 (MQ=255)
                                         ggCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTACGACAGCCTCATCCCAAAGCAGGCAAggg                                                                                                              >  2:552512/1‑150 (MQ=255)
                                          gCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGGTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGa                                                                                                             <  1:851561/150‑1 (MQ=255)
                                           cAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACAAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGAGTGTGAAGATTCGGTGTCGGCAAAACTTTTCAGACAGCCTCCTCCAAAAGCAGGCAAGGGaa                                                                                                            >  1:1025780/1‑150 (MQ=255)
                                           cAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTACAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGaa                                                                                                            >  1:185047/1‑150 (MQ=255)
                                             gCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAAt                                                                                                          >  1:171838/1‑150 (MQ=255)
                                               gAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGCTCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAATCTTTTCCGACAGCCTCATCCCAACGCAGGCAAGGGAAATgg                                                                                                        >  2:334037/1‑150 (MQ=255)
                                               gAAGTGCAGCTGGTCGCTGAAGGCGCGGTCAGTTGCTCAAGTGCAATACCAGATTACGCCAGTTGATCCCGGGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGGCAGCCTCATCCCAAAGCAGGCAAGGGAAATgg                                                                                                        <  2:1352093/150‑1 (MQ=255)
                                                atgTGCTGCTGGTCGCAGAAGGCGCGTTCTGTTGCTCAATTGCAATACGAGATTTCGCGAGTTGATCCCGTGCCTGTATCAGCGTGTGTAGATTCGGTGTCTGGAAAACTTTTCCGTCAGCCTCATCCCAATGCAGGCTATGGAAATtgg                                                                                                       <  1:1468208/148‑1 (MQ=255)
                                                aaGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATggg                                                                                                       <  2:1514121/150‑1 (MQ=255)
                                                      aGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTc                                                                                                 >  1:1075399/1‑150 (MQ=255)
                                                      aGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTACGTGTCGCCAAAACTATTACAACATCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTc                                                                                                 >  2:1772849/1‑150 (MQ=255)
                                                          ggTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGc                                                                                             >  1:1929448/1‑150 (MQ=255)
                                                          ggTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGc                                                                                             <  2:1791585/150‑1 (MQ=255)
                                                          ggTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGc                                                                                             <  2:1685720/150‑1 (MQ=255)
                                                            tCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTa                                                                                           <  1:1576649/150‑1 (MQ=255)
                                                            tCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTa                                                                                           <  1:1568837/150‑1 (MQ=255)
                                                              gCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTaaa                                                                                         <  2:2138076/150‑1 (MQ=255)
                                                               cTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAAt                                                                                        >  1:892049/1‑150 (MQ=255)
                                                                tGAAGGCGCGTTCAGTTGCTCAAGTGCATTACCAGATTTCGCCAGTTGATCCCGTGCCGGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAACGGAAATGGGTAAGTCCGGCTAAATg                                                                                       >  1:1988860/1‑150 (MQ=255)
                                                                 gAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCTACAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCACGCAAGGGAAATGGGTAAGTCCGGCTAAATGc                                                                                      <  1:124038/150‑1 (MQ=255)
                                                                    ggCGCGTTCAGTTGCTCAAGTGCAAAACCAGATTTCGCCACTTGATCCCGAGCCTGTATCAGCGTGTGAAGATTAGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAAGGCAGc                                                                                   >  1:402152/1‑150 (MQ=255)
                                                                       gcgTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCt                                                                                <  1:464000/150‑1 (MQ=255)
                                                                        cgTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGCTTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTg                                                                               <  1:1425343/150‑1 (MQ=255)
                                                                          ttCAGTTGCTCAAGTGCAATACAAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACATTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAGATGCAGCCCTGGc                                                                             >  1:1767848/1‑150 (MQ=255)
                                                                           tCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCa                                                                            <  2:2137122/150‑1 (MQ=255)
                                                                           tCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCa                                                                            <  2:1055366/150‑1 (MQ=255)
                                                                               ttGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGAtt                                                                        <  2:1117668/150‑1 (MQ=255)
                                                                                tGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTc                                                                       <  2:1442541/150‑1 (MQ=255)
                                                                                 gCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGAATCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCt                                                                      >  1:1023556/1‑150 (MQ=255)
                                                                                  cTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGCATCTg                                                                     >  1:1789642/1‑150 (MQ=255)
                                                                                  cTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTg                                                                     <  1:1561965/150‑1 (MQ=255)
                                                                                      aGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATAACGTTTCTCCAAAAATAATAAAAAAAACACATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGAtt                                                                 >  2:652404/1‑150 (MQ=255)
                                                                                        tGCAATACCAGATTTCGCCATTTCATCCCGTGCCTGAATCAGCGTGTGAAGATTCGGTCTCGGCAAAACTTTTCCGACAGCCTCATCACAAAGCAGGCAAGGGAAATGTGTAAGTCCGGCTAAATGCAGCCCTGTCAGATTCTGGATTTc                                                               <  1:1154129/150‑1 (MQ=255)
                                                                                            aTACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCg                                                           <  2:254163/150‑1 (MQ=255)
                                                                                            aTACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCg                                                           <  1:166005/150‑1 (MQ=255)
                                                                                            aTACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCg                                                           >  1:2039451/1‑150 (MQ=255)
                                                                                              aCCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGAc                                                         <  1:2121216/150‑1 (MQ=255)
                                                                                               ccAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACa                                                        >  1:1635149/1‑150 (MQ=255)
                                                                                               ccAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACa                                                        >  2:844917/1‑150 (MQ=255)
                                                                                                  gATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAAtc                                                     >  1:1552527/1‑150 (MQ=255)
                                                                                                   aTTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATAACGTTTCTCCAAAACTAATAAAACAACCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCGGGCAGATTCGGGATTTCGGCGACATtcg                                                    >  2:2088196/1‑149 (MQ=255)
                                                                                                    tttCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAAtctc                                                   >  1:930282/1‑150 (MQ=255)
                                                                                                      tCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTACCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAGTCTCCg                                                 >  1:2191552/1‑150 (MQ=255)
                                                                                                      tCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTGTGCCGACAGCCTCATCCCACAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCg                                                 >  1:2199944/1‑150 (MQ=255)
                                                                                                      tCGACAGTGGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCTAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCg                                                 <  1:1636587/150‑1 (MQ=255)
                                                                                                        gCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCGGc                                               <  2:767693/150‑1 (MQ=255)
                                                                                                        gCCAGTTGATCACGTGCCTTTATCTGGGTTTTAAGATTCGGTGTCTGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCGTGGCAGATTCTGGATTTCGGCGACAATCTCCGGc                                               <  2:1025780/150‑1 (MQ=255)
                                                                                                               gATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGTCTATACTTTTCCGAGAGCCTCTTCCCAAAGCAGGCAAGGGAAATGGGTTTGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCGGCTACACCt                                        <  2:847427/150‑1 (MQ=255)
                                                                                                                 tCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCGGCAACTCCTTg                                      <  2:1674517/150‑1 (MQ=255)
                                                                                                                 tCCCGTGCCTGTATCAGCGTGTGAAGATAACGTTTCTCCAAAAATAATAAAAAAAAAAAATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCGGCAACACCTTg                                      >  2:512936/1‑150 (MQ=255)
                                                                                                                    cGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCGGCAACACCTTGAgg                                   <  2:849539/150‑1 (MQ=255)
                                                                                                                     gTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCGGCAACACCTTGAggg                                  <  2:1075399/150‑1 (MQ=255)
                                                                                                                      tGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCGGCAACACCTTGAGGGc                                 <  2:1929448/150‑1 (MQ=255)
                                                                                                                       gCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCGGCAACACCTTGAGGGCa                                <  1:665559/150‑1 (MQ=255)
                                                                                                                           gTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCATAGCAGGCAAGGGAAATGGGTTAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCGGCAACACCTTGAGGGCAAAAc                            <  2:1712964/150‑1 (MQ=255)
                                                                                                                           gTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCGGCAACACCTTGAGGGCAAAAc                            <  2:1192567/150‑1 (MQ=255)
                                                                                                                           gTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCGGCAACACCTTGAGGGCAAAAc                            <  2:726049/150‑1 (MQ=255)
                                                                                                                            tATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCGGCAACACCTTGAGGGCAAAAcc                           <  2:2137328/150‑1 (MQ=255)
                                                                                                                            tATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCGGCAACACCTTGAGGGCAAAAcc                           <  2:1590807/150‑1 (MQ=255)
                                                                                                                              tcatcttgtgTAGATTAGTTGTCGGCAAAAATTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCGGAAACACCTTGAGGGCAAAACCGc                         <  2:1622716/144‑1 (MQ=255)
                                                                                                                              tCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGCCAGCCTCCTCCCAAAGCAGGCAAGGGAATTGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCGGCAACACCTTGAGGGCAAAACCGc                         >  1:1786290/1‑150 (MQ=255)
                                                                                                                                   gtgtGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTTAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCGGCAACACCTTGAGGGCAAAACCGCTCTcc                    <  2:1719296/150‑1 (MQ=255)
                                                                                                                                    tgtgAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCGGCAACACCTTGAGGGCAAAACCGCTCTCCt                   >  1:1691365/1‑150 (MQ=255)
                                                                                                                                    tgtgAAGATTAGGTTTCGCCAAAAATTATCAAAAAACCACATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGCCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGAGACAATCTCCGGCAAAACCTTGCGGGCAAAACCGCTCTCCt                   >  2:900748/1‑150 (MQ=255)
                                                                                                                                     gtgAAGATTCGGTGTCGGCAAAACTTTTACGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTATGGATTTCGGCGACAATCTCCGGCAACACCTTGAGGGCAAAACCGCTCTCCTg                  >  2:325517/1‑150 (MQ=255)
                                                                                                                                     gtgAAGATACGGGGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGCAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCGGCAACACCTTGAGGGCAAAACCGCTCTCCTg                  <  2:1285908/150‑1 (MQ=255)
                                                                                                                                       gAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCGGCAACACCTTGAGGGCAAAACCGCTCTCCTGGc                <  1:1942766/150‑1 (MQ=255)
                                                                                                                                          gATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTATGTCCGGCTAAATGCAGCCGTGGCAGCTTCTGGATTTCGGCGACAATCTCCGGCAACACCTTGAGGGCAAAACCGCTCTCCTGGCCCg             >  1:254021/1‑150 (MQ=255)
                                                                                                                                          gATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCGGCAACACCTTGAGGGCAAAACCGCTCTCCTGGCCCg             <  1:585935/150‑1 (MQ=255)
                                                                                                                                           aTTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATTCAGCCCTGGCTGATTCTGGATTTCGGCGACAATCTCCGGCAACACCTTGAGGGCAAAACCGCTCTCCTGGCCCgg            >  2:861875/1‑150 (MQ=255)
                                                                                                                                             tCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGGTAAATGCAGCCCTGGCAGTTTCTGGATTTCGGCGACAATCTCCGGCAACACCTTTAGGGCAAAACCGCTCTCCTGGCCCGGAt          <  2:185047/150‑1 (MQ=255)
                                                                                                                                             tCGGAGTCGGAAAAACTTATACGACTTCCTCATACCAAAGCAGGCAAGGGAAATGTTTAAGTCAGGCTAAATGCAGCCCTGGCAGATTCTTGATTTCGGCGACAATATCCGGCAACACCTTGAGTGCAAAACCGCTCTCCTGGCCCGGac          <  1:1389938/150‑2 (MQ=255)
                                                                                                                                              ggctgtCGGAAAAGTTTTGCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCGGCAACACCTTGAGGGCAAAACCGCTCTCCTGGCCCGGAtt         >  1:317789/4‑149 (MQ=255)
                                                                                                                                                    cGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCGGCAACACCTTGAGGGCAAAACCGCTCTCCTGGCCCGGATAAAGaaa   >  1:731766/1‑150 (MQ=255)
                                                                                                                                                    cGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGACCGGCTAAATGCAGCCCTGGCAGAATCTGGATTTCGGCGACAATCTCCGGCAACACCTAGAGGGCAATAGCGCTCTCCTGGCCCGGATAAAGaaa   <  1:272570/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GACCGGGTTCGGCATGAGTCACACCGTATTGCGCCAGTAATGGCAGCGAAGTGCAGCTGGTCGCTGAAGGCGCGTTCAGTTGCTCAAGTGCAATACCAGATTTCGCCAGTTGATCCCGTGCCTGTATCAGCGTGTGAAGATTCGGTGTCGGCAAAACTTTTCCGACAGCCTCATCCCAAAGCAGGCAAGGGAAATGGGTAAGTCCGGCTAAATGCAGCCCTGGCAGATTCTGGATTTCGGCGACAATCTCCGGCAACACCTTGAGGGCAAAACCGCTCTCCTGGCCCGGATAAAGAAAA  >  NC_000913/3510208‑3510506

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: