Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,530,673 G→C 7.3% intergenic (+21/+42) hslO → / ← yhgE heat shock protein Hsp33/DUF4153 family putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,530,6730GC 7.3% 462.1 / 24.8 150intergenic (+21/+42)hslO/yhgEheat shock protein Hsp33/DUF4153 family putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (69/69);  new base C (5/6);  total (74/76)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.85e-01

Only 100 of 157 total aligned reads displayed.

AGCATCCTGGCGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCTGTGAGTAATTTTTC  >  NC_000913/3530524‑3530822
                                                                                                                                                     |                                                                                                                                                     
aGCATCCTGGCGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAgg                                                                                                                                                       <  1:908830/150‑1 (MQ=255)
aGCATCCTGGCGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAgg                                                                                                                                                       <  1:196579/150‑1 (MQ=255)
 ccacccTGGCGGAAGACGGCGAAAATGACATCCATTGTTAATACTGCGGTAACCACTCTCTGTGCAAGGCGATGGAACTTGCTGAAATCCGCCACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAggg                                                                                                                                                      <  2:898435/146‑1 (MQ=255)
  cATCCTGGCGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGc                                                                                                                                                     <  2:555244/150‑1 (MQ=255)
      cTGGCGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTg                                                                                                                                                 <  2:1877326/150‑1 (MQ=255)
      cTGGCGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTg                                                                                                                                                 <  1:27227/150‑1 (MQ=255)
         gCGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGCCCTCTCTGCCg                                                                                                                                              <  1:1125547/150‑1 (MQ=255)
         gCGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGCCCTCTCTGCCg                                                                                                                                              <  2:1029493/150‑1 (MQ=255)
         gCGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCg                                                                                                                                              <  1:1933521/150‑1 (MQ=255)
         gCGGAAGATGGCGAAATGGACATGCTTTGTGATTACTGCGGTAACCACTAGCTGTTGAATGCGATGGATATTGCTGAAATCCGCAACAACGCGGCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGCCCTCTCTGCCg                                                                                                                                              <  1:93047/150‑1 (MQ=255)
          cGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGCCCTCTCTGCCgg                                                                                                                                             <  2:2212262/150‑1 (MQ=255)
           ggAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGCCCTCTCTGCCGGa                                                                                                                                            <  2:657907/150‑1 (MQ=255)
              aGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGAttt                                                                                                                                         <  2:2033791/150‑1 (MQ=255)
                 tGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGTATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTAc                                                                                                                                      <  1:1002639/150‑1 (MQ=255)
                 tGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTAc                                                                                                                                      <  2:1920890/150‑1 (MQ=255)
                  ggCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACg                                                                                                                                     <  1:576604/150‑1 (MQ=255)
                  ggCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACg                                                                                                                                     <  2:1391801/150‑1 (MQ=255)
                  ggCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACg                                                                                                                                     <  2:538462/150‑1 (MQ=255)
                    cGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGAc                                                                                                                                   <  1:373742/150‑1 (MQ=255)
                    cGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGAc                                                                                                                                   <  1:239951/150‑1 (MQ=255)
                    agAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGATGGCTCTGCCGGATTTTACGAc                                                                                                                                   >  1:926293/2‑150 (MQ=255)
                     gAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCTATGGATATTGCTGAAATCCGCAACAACGCGACTCAGGCAGATCCGCAAGATCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGAATTTACGACt                                                                                                                                  >  1:98978/1‑150 (MQ=255)
                        aTTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGACATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAAt                                                                                                                               <  2:1320127/150‑1 (MQ=255)
                          tGACATGCATTGTGATTACTGCGGTAACCACTATGTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTGCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAAGac                                                                                                                             >  2:351189/1‑150 (MQ=255)
                           gACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATacc                                                                                                                            >  2:1973182/1‑149 (MQ=255)
                             cATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACAcc                                                                                                                          >  1:943425/1‑150 (MQ=255)
                                gCATTGTGATTAATGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTCCTTTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGa                                                                                                                       <  2:2209276/150‑1 (MQ=255)
                                         ttACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTg                                                                                                              <  1:787364/150‑1 (MQ=255)
                                         ttACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTg                                                                                                              >  1:2096364/1‑150 (MQ=255)
                                           aCTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTc                                                                                                            >  1:2210267/1‑150 (MQ=255)
                                           aCTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTc                                                                                                            >  1:984741/1‑150 (MQ=255)
                                            cTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCa                                                                                                           >  1:531973/1‑150 (MQ=255)
                                                  tAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCAtt                                                                                                     <  1:1967749/150‑1 (MQ=255)
                                                   aaCCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTc                                                                                                    <  2:2057582/150‑1 (MQ=255)
                                                    actaCTATCTGTTCATTGCGATGGATATTGGTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGAAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCa                                                                                                   <  2:433012/147‑1 (MQ=255)
                                                    aCCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCa                                                                                                   <  1:1992634/150‑1 (MQ=255)
                                                    aCCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCa                                                                                                   <  2:1955509/150‑1 (MQ=255)
                                                     ccACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAg                                                                                                  >  2:2173376/1‑150 (MQ=255)
                                                     ccACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAg                                                                                                  >  2:1104952/1‑150 (MQ=255)
                                                         tATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTc                                                                                              <  2:2098795/150‑1 (MQ=255)
                                                           tCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTcac                                                                                            <  2:537634/150‑1 (MQ=255)
                                                                tCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTCATGCGCTTTGTCACTCATTCAGATTCACATTCa                                                                                       >  2:1700621/1‑150 (MQ=255)
                                                                 cAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAg                                                                                      >  2:507320/1‑150 (MQ=255)
                                                                  aaTGCGATGGATATTGCTGAAATCCGCAACAACGCGACTCCCGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATATTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGc                                                                                     >  1:1525197/1‑150 (MQ=255)
                                                                    tGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCAGTAATGAGTATGTCCGGAAGAGAGGGCTCTGCCGGATTTTACGACTAATACCCCTGATGCGCTTTGTCACTCATTCAGAGTCACATTCAGCCt                                                                                   >  2:1366517/1‑150 (MQ=255)
                                                                       gATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGTCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTc                                                                                <  1:1669212/150‑1 (MQ=255)
                                                                        aTGGATATTGCTGAACTCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACCACTAATACACCTGATGCGCTATGTCAGTCATTCCGATTCACATTCAGCCTTTcc                                                                               >  2:135584/1‑149 (MQ=255)
                                                                           gATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCa                                                                            <  1:1913346/150‑1 (MQ=255)
                                                                             tatTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATc                                                                          >  1:1047566/1‑150 (MQ=255)
                                                                              tttGCTGAAATCCGCAACCACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGGCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGAGCTTTGTCACTCATTCAGATTCCCATTCAGCCTTTCACCATcc                                                                         <  2:1045570/149‑1 (MQ=255)
                                                                                tGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCAc                                                                       >  1:1819597/1‑150 (MQ=255)
                                                                                tGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCAc                                                                       <  1:1478173/150‑1 (MQ=255)
                                                                                tGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCAc                                                                       <  1:699496/150‑1 (MQ=255)
                                                                                 gCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCTGCAGAGAGGTCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACt                                                                      <  1:362777/150‑1 (MQ=255)
                                                                                 gCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGTCTCTGCCGGATTTTACGACTAATACACCTGATGCTCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACt                                                                      <  1:335576/150‑1 (MQ=255)
                                                                                  cTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTa                                                                     >  1:2007243/1‑150 (MQ=255)
                                                                                        tCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGAt                                                               <  2:1965096/150‑1 (MQ=255)
                                                                                         ccGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAatata                                                              >  1:1622882/1‑149 (MQ=255)
                                                                                         ccGAAACAACGCGTATCCTGCAGATCAGCAAGTTCATTAATGAGTATGTCCGTCAGAGACGGCTCTGCCGGATGTTACGACTAATACACCTGATGCGCTTTGTCCCTAATTCATAATCCCATTCACCCATTCACACTCCAATaaaataaa                                                              >  2:1585116/1‑146 (MQ=255)
                                                                                           gCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATcgc                                                            >  1:1277968/1‑150 (MQ=255)
                                                                                            caacaaCGCGTCTCCGGCAGATCCGCAAGGTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGCTTCACATTCAGCCTTTCACCATCCCCTACAAGATcgcg                                                           >  2:1763888/1‑150 (MQ=255)
                                                                                             aacaacGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCgcgc                                                          >  2:747129/1‑150 (MQ=255)
                                                                                              acaacGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTATACGACTAAAACACCTGATGCGCTTTGTCACTCATACAGATTCACATTCAGCCTTTCA‑‑ATCCACTACAAGATCGCGCCAc                                                       >  1:409750/1‑150 (MQ=255)
                                                                                                   gcgTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCt                                                    <  1:2204814/150‑1 (MQ=255)
                                                                                                       ctcCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAg                                                >  1:1666605/1‑150 (MQ=255)
                                                                                                         ccGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGc                                              >  1:453925/1‑150 (MQ=255)
                                                                                                          cGGCAGATCCGCATGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCt                                             <  1:2042264/150‑1 (MQ=255)
                                                                                                          cGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCt                                             >  1:1454698/1‑150 (MQ=255)
                                                                                                          cGGCAGATCAGCAAGTTCATTAATGAGTATGTCCGGCAGAGCGGGCTCTGCCCGATTTTACGACTAATAAACCTGATCCGCTTTCTCACTCATTAAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCt                                             >  2:1537882/1‑150 (MQ=255)
                                                                                                                aTCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCTCCACGCTTTAGGCTTCGTcc                                       <  1:1215383/150‑1 (MQ=255)
                                                                                                                  ccGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTcccc                                     <  2:2041225/150‑1 (MQ=255)
                                                                                                                  ccGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTcccc                                     <  2:920283/150‑1 (MQ=255)
                                                                                                                  ccGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGACCCTCATTCCGATTCACATTCAGGCTTTCACCATCCACTACAAGATCGCGCCACGCGTTAGGCGTCGTcccc                                     >  2:62399/1‑150 (MQ=255)
                                                                                                                   cGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCa                                    <  2:1208966/150‑1 (MQ=255)
                                                                                                                    gCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAg                                   <  2:209675/150‑1 (MQ=255)
                                                                                                                     cAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTTATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTGTAGGCTTCGTCCCCAGt                                  >  2:315245/1‑150 (MQ=255)
                                                                                                                         ttCATTAATGAGTTTGTCCGGCAGTGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTc                              <  2:393868/150‑1 (MQ=255)
                                                                                                                          tCATTAATGAGTATGTCCGGGAGTGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTcc                             <  1:2181911/150‑1 (MQ=255)
                                                                                                                            aTTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCAt                           >  1:1525449/1‑150 (MQ=255)
                                                                                                                            aTTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCAt                           <  2:242615/150‑1 (MQ=255)
                                                                                                                            aTTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCAt                           <  2:2007243/150‑1 (MQ=255)
                                                                                                                             ttAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATc                          <  2:1787767/150‑1 (MQ=255)
                                                                                                                                  gAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCg                     >  1:1681577/1‑150 (MQ=255)
                                                                                                                                   aGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGc                    <  2:897595/150‑1 (MQ=255)
                                                                                                                                       tGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCt                >  2:750907/1‑150 (MQ=255)
                                                                                                                                        gTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCtg               >  2:1646936/1‑150 (MQ=255)
                                                                                                                                        gTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATACACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCtg               <  1:705122/150‑1 (MQ=255)
                                                                                                                                         tCCGGCAGAGCCCTCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCtgt              >  1:1011159/1‑150 (MQ=255)
                                                                                                                                          ccGGCAGAGCCCTCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCtgtg             >  1:2124003/1‑150 (MQ=255)
                                                                                                                                          ccGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATCCACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCtgtg             >  1:1447822/1‑150 (MQ=255)
                                                                                                                                          ccGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCtgtg             <  1:2173376/150‑1 (MQ=255)
                                                                                                                                          ccGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCtgtg             >  2:1644360/1‑150 (MQ=255)
                                                                                                                                          ccGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCAATCCTTCGCAGCtgtg             >  1:760043/1‑150 (MQ=255)
                                                                                                                                           cGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCTGTGa            >  2:460049/1‑150 (MQ=255)
                                                                                                                                            ggCAGAGATTGCTCTTCCGGATTTTACTACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTAGAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCTCAGCTGTGAg           <  1:245648/150‑1 (MQ=255)
                                                                                                                                            ggCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTCATGCGCTTTGTCACTCATTCAGAATCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCTGTGAg           <  2:427438/150‑1 (MQ=255)
                                                                                                                                             gCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCATCTTCACAATCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCTGTGAGt          <  2:2073060/150‑1 (MQ=255)
                                                                                                                                             gCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCTGTGAGt          >  1:937648/1‑150 (MQ=255)
                                                                                                                                                gagagGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTAACATTCAGCCTATCACCATCCACTACAAGATCGCGCCCCGCTTTAGGCTTCGTCCCAAGGTTTCCATCCTTCGCAGCTGTGaataat       >  2:215253/1‑150 (MQ=255)
                                                                                                                                                     ggCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCTGTGAGTAATTtttc  <  2:1835070/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGCATCCTGGCGGAAGATGGCGAAATTGACATGCATTGTGATTACTGCGGTAACCACTATCTGTTCAATGCGATGGATATTGCTGAAATCCGCAACAACGCGTCTCCGGCAGATCCGCAAGTTCATTAATGAGTATGTCCGGCAGAGAGGGCTCTGCCGGATTTTACGACTAATACACCTGATGCGCTTTGTCACTCATTCAGATTCACATTCAGCCTTTCACCATCCACTACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCTGTGAGTAATTTTTC  >  NC_000913/3530524‑3530822

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: