Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,537,323 T→A 5.8% intergenic (+35/‑62) greB → / → yhgF transcript cleavage factor/putative transcriptional accessory protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,537,3230TA 5.8% 406.8 / 9.2 134intergenic (+35/‑62)greB/yhgFtranscript cleavage factor/putative transcriptional accessory protein
Reads supporting (aligned to +/- strand):  ref base T (80/46);  new base A (6/2);  total (86/48)
Fisher's exact test for biased strand distribution p-value = 7.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 138 total aligned reads displayed.

CCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCTGCTTG  >  NC_000913/3537176‑3537469
                                                                                                                                                   |                                                                                                                                                  
ccccGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGTCTGGCAtttt                                                                                                                                                  >  1:1016932/1‑150 (MQ=255)
ccccGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCAtttt                                                                                                                                                  >  2:2071168/1‑150 (MQ=255)
ccccGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATAACCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCAtttt                                                                                                                                                  >  1:536986/1‑150 (MQ=255)
  ccGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTg                                                                                                                                                >  1:1177363/1‑150 (MQ=255)
  ccGATGGCCCACGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTg                                                                                                                                                >  2:1478498/1‑150 (MQ=255)
      tGGCCCGCGCATTGCTGAAAAACGAAGTCGGCGATCTGGCGGTGGTTAAAACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGATTCTTAACCTCCTGCCGATGGCTGGCATTTTTgccag                                                                                                                                            <  1:1999054/150‑1 (MQ=255)
        gCCCGCGCATTGCTGAAACATGAAGTCGGCGATCTGGCGTTTGTTAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTgccagcc                                                                                                                                          <  2:1319461/150‑1 (MQ=255)
         cccGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTgccagcca                                                                                                                                         >  2:45449/1‑150 (MQ=255)
           cgcgCATTGCTGAAAAAAGAAGTCGGCTATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCAAAAATgccagccatc                                                                                                                                       <  1:1699724/150‑3 (MQ=255)
                   gCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTCTCGAGTACGTGAAACCGTAAGGAAGAGTCTAAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCATACCGTATaa                                                                                                                               >  2:1051786/1‑150 (MQ=255)
                   gCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATaa                                                                                                                               >  2:1333163/1‑150 (MQ=255)
                   gCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATaa                                                                                                                               >  2:631929/1‑150 (MQ=255)
                   gCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATaa                                                                                                                               >  1:1480348/1‑150 (MQ=255)
                    cTGAAAAATGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTACCCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAAc                                                                                                                              >  1:152895/1‑150 (MQ=255)
                    cTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTTTGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGGATTTTTGCCAGCCAGTCCGTATaaa                                                                                                                              >  1:1856103/1‑149 (MQ=255)
                    cTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAAc                                                                                                                              >  1:70860/1‑150 (MQ=255)
                      gAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCGGGCCTTTTTGCCAGCCAGTACGTATAACTa                                                                                                                            >  2:1670397/1‑150 (MQ=255)
                       aaaaaaGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTAt                                                                                                                           >  2:1121921/1‑150 (MQ=255)
                       aaaaaaGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTAt                                                                                                                           >  2:1553715/1‑150 (MQ=255)
                           acgatgTCGGCGAGCTGGCGGTGGTGAATAGCCCTGCCGGGGAAGCGAGCTGGTATGTTTATGCTATCGAGTACGTGAATCGGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATcacc                                                                                                                       <  2:200182/145‑1 (MQ=255)
                                  cGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTTCCAGACAGTACGTATAACGAACCCCGGAtttt                                                                                                                >  2:880609/1‑150 (MQ=255)
                                  cGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGAtttt                                                                                                                >  2:70264/1‑150 (MQ=255)
                                    gCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTg                                                                                                              <  2:698236/150‑1 (MQ=255)
                                       aTCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTTCCAGCCAGTCCCTATAACTATCCCCTGATTTTTTATc                                                                                                           >  2:454871/1‑150 (MQ=255)
                                       aTCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATc                                                                                                           <  1:581504/150‑1 (MQ=255)
                                           ggCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGCGTCTTAACCTCCTGCCGCTGGCTGGCATTTTTGCCAGCAAGTCAGTATAACTATACCCTGATTTTTGATCAGaa                                                                                                       >  1:336089/1‑150 (MQ=255)
                                           ggCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGaa                                                                                                       >  1:639590/1‑150 (MQ=255)
                                             cGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGaaaa                                                                                                     >  2:1191000/1‑150 (MQ=255)
                                               gtggggAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGa                                                                                                   <  1:1539929/150‑1 (MQ=255)
                                                 ggtgAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATg                                                                                                 <  1:2213710/150‑1 (MQ=255)
                                                  gtgAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGa                                                                                                >  1:1150058/1‑150 (MQ=255)
                                                    gAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAAc                                                                                              <  2:978610/150‑1 (MQ=255)
                                                    gAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAAc                                                                                              >  2:1993705/1‑150 (MQ=255)
                                                     aaTACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACt                                                                                             >  1:1779545/1‑150 (MQ=255)
                                                         ccccTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGTCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATATTTCATCCGAAAACATGAACTCaaa                                                                                         >  2:1243847/1‑150 (MQ=255)
                                                         ccccTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGAttttt                                                                                                               >  1:941587/1‑128 (MQ=255)
                                                         ccccTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGCACTCaaa                                                                                         >  1:292273/1‑150 (MQ=255)
                                                         ccccTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCaaa                                                                                         >  2:564443/1‑150 (MQ=255)
                                                         ccccTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCaaa                                                                                         >  2:978985/1‑150 (MQ=255)
                                                          cccTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATTGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGAACCGAAAAGATGAAATAAAAc                                                                                        >  2:1702341/1‑150 (MQ=255)
                                                            cTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCACTGATTTTTGATCCGAAAAGATGAACTCAAACCa                                                                                      >  1:1278164/1‑150 (MQ=255)
                                                                cGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCatgat                                                                                  <  1:945485/150‑1 (MQ=255)
                                                                cGGGGAAGCGAGCGGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTACCCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCatgat                                                                                  <  2:289413/150‑1 (MQ=255)
                                                                   ggAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGatgaa                                                                               <  1:553794/150‑1 (MQ=255)
                                                                     aaGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTTCCAGCCAGTCCGTATAACTATCCCCGGATTTTTGATCCGAAAAGATGAACAAAAACCATGatgaatg                                                                             >  1:819381/1‑150 (MQ=255)
                                                                      aGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGatgaatga                                                                            >  2:857744/1‑150 (MQ=255)
                                                                        cGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGAtt                                                                          >  2:1715020/1‑150 (MQ=255)
                                                                           gCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATTGCATGCATTTTAGCCAGCCAATCCCTATAAAAATACCCTGAATTTTGATAAGAAAAAATTAAAACAAACCAAGattaatgaatact                                                                       >  1:1406784/1‑145 (MQ=255)
                                                                                tATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGc                                                                  <  2:686398/150‑1 (MQ=255)
                                                                                  tGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATTGCTGGCATTTTTGCCAGCCCGTCCGTATAACGATCCCCTGATTTTTGATCCGAAAAGATTAACTCAAACCCTGATGAATGAGTCGTTCTGCCg                                                                >  2:1668269/1‑150 (MQ=255)
                                                                                  tGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCg                                                                >  2:1495938/1‑150 (MQ=255)
                                                                                  tGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCg                                                                <  2:1915433/150‑1 (MQ=255)
                                                                                      aaTGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTAGTTCTGCCGCatt                                                            >  1:703724/1‑150 (MQ=255)
                                                                                       aTGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCattt                                                           >  2:773857/1‑149 (MQ=255)
                                                                                        tGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGCTCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCattat                                                          >  2:491357/1‑150 (MQ=255)
                                                                                         gCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGCTTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCattatt                                                         >  1:2094966/1‑150 (MQ=255)
                                                                                         gCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCattatt                                                         >  2:427945/1‑150 (MQ=255)
                                                                                         gCTATCGAGTACGCGAAACCGTAAGGAAGAGTCTTACCCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTTTTCCTGTGCCCTGATTTTTGATCCGAAAAGATGACCTCAAACCATGATGATGGATTCGTTCTGCCGCattatt                                                         >  2:1078773/1‑150 (MQ=255)
                                                                                          cTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGGATAACTAAACCCTGATTTTTGATCCGAAAAGATGCACTCAAACCATGAATAATGATTCGTTCTGCCTCATTATTg                                                        >  1:1354054/1‑150 (MQ=255)
                                                                                           tATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGc                                                       <  2:369875/150‑1 (MQ=255)
                                                                                            aTCTAGTACGTGAAACCGTAAGGAAGATTCTTAACCTCCTGCCGATGGCTGTCATTTTTGCCAGACAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCg                                                      <  1:209458/150‑1 (MQ=255)
                                                                                              cGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATTAACTCAACCCATGATGAATGATTCGTTCTGCCGCATTATTGCggg                                                    >  1:161793/1‑150 (MQ=255)
                                                                                              cGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCggg                                                    <  1:706307/150‑1 (MQ=255)
                                                                                              cGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCAGGCATTTTTGCCAGCCAGTCCGTATAACAATACCCTGTTTTTTGTTCCGAAAAGATGAAAGCAAACCATGATGAATGATTCTTTAAGCAGCATTATTGCggg                                                    >  2:857308/1‑150 (MQ=255)
                                                                                                 gTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCCGCCAGTCCGTATAACTATACCCTGATTTTTGATACGAAAAGATGAACGCAAACCATGATTAAAGATTAGTTCTGCCGCATTATTTCGGGTGa                                                 >  1:2198132/1‑150 (MQ=255)
                                                                                                    cGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAAt                                              >  2:1214562/1‑150 (MQ=255)
                                                                                                    cGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTACCCGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAAt                                              >  1:525254/1‑150 (MQ=255)
                                                                                                      tGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCAAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTTCGGGTGAAATTc                                            >  1:87438/1‑150 (MQ=255)
                                                                                                       gAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCa                                           >  2:37787/1‑150 (MQ=255)
                                                                                                           ccGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGgcg                                       <  1:2120738/150‑1 (MQ=255)
                                                                                                           ccGTAAGGAAGAGTATTAACCTCCTGCCGATGGCTGGCATTTTTGCCTGCCAGTCCGTAAAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGgcg                                       <  2:1394402/150‑1 (MQ=255)
                                                                                                            cGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGAGTGTTGATCCGAAAAGATGAGCTCAAACCATGATGAATGATTCGTTCTGACGCATTATTGCTGGTGAAATTCATgcgc                                      >  1:385249/1‑150 (MQ=255)
                                                                                                                 ggAAGAGTCTAAACCTCCTGCCGATGTCTGGCATATTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGTGTGAAATTCAGGCGCGCCCg                                 <  2:1125830/150‑1 (MQ=255)
                                                                                                                  gAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCgg                                >  1:890384/1‑150 (MQ=255)
                                                                                                                   aaGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCggg                               >  1:1075436/1‑149 (MQ=255)
                                                                                                                   aaGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGa                               <  1:2020748/150‑1 (MQ=255)
                                                                                                                     gagTCTTAACATCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACAATCCCCGTAGTTTTGATCCGAAAAGATGCACTCAACACATGATGAATGATTCTGTCTGCCGCATTATTGAGGGTTAAATTCAGGCGAGCCAggcaa                             >  2:207629/1‑147 (MQ=255)
                                                                                                                      cgTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACa                            <  1:1114749/149‑1 (MQ=255)
                                                                                                                      agTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACa                            <  1:1121921/150‑1 (MQ=255)
                                                                                                                        tCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAgg                          >  2:2147276/1‑150 (MQ=255)
                                                                                                                         catAACCCCCAGCCGTAGGCTGGGCTTTTTTCCATGCAGTCCCTATTAAAATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGt                         <  1:1478498/148‑1 (MQ=255)
                                                                                                                         cTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGt                         >  2:484379/1‑150 (MQ=255)
                                                                                                                           tAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTAAAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTg                       >  2:205294/1‑150 (MQ=255)
                                                                                                                                  tacggactggctggcAAAAATGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTTTTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGc                >  1:562819/8‑150 (MQ=255)
                                                                                                                                  ctGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGc                <  1:1728092/150‑1 (MQ=255)
                                                                                                                                    gCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTTCTTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTGCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCg              <  1:1152246/150‑1 (MQ=255)
                                                                                                                                    gCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCg              >  2:1053890/1‑150 (MQ=255)
                                                                                                                                    cccGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCg              >  1:1752172/2‑150 (MQ=255)
                                                                                                                                     ccGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGt             >  2:2199246/1‑150 (MQ=255)
                                                                                                                                        ctggctggcAAAAATGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCg          >  1:732623/2‑150 (MQ=255)
                                                                                                                                        ctggctggcAAAAATGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCg          >  1:775335/2‑150 (MQ=255)
                                                                                                                                        aTGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCg          <  1:857744/150‑1 (MQ=255)
                                                                                                                                         tggctggcATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGc         <  2:744114/150‑1 (MQ=255)
                                                                                                                                           gctggcATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCt       <  1:2071168/150‑1 (MQ=255)
                                                                                                                                            ctggcATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCTg      >  2:551025/1‑150 (MQ=255)
                                                                                                                                            ctggcATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCTg      >  1:1447126/1‑150 (MQ=255)
                                                                                                                                            ctggcATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTAAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTCAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCTg      >  1:680278/1‑150 (MQ=255)
                                                                                                                                             tggcATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCTGc     >  1:927706/1‑150 (MQ=255)
                                                                                                                                              ggcATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCTGCt    <  1:820340/150‑1 (MQ=255)
                                                                                                                                                cATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCTGCTTg  <  1:1164821/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCGGCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAAGGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAAAAGATGAACTCAAACCATGATGAATGATTCGTTCTGCCGCATTATTGCGGGTGAAATTCAGGCGCGCCCGGAACAGGTTGACGCTGCCGTTCGCCTGCTTG  >  NC_000913/3537176‑3537469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: