Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,636,008 G→T 5.0% intergenic (+115/+200) yhiM → / ← yhiN acid resistance protein, inner membrane/putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,636,0080GT 5.0% 397.6 / 6.1 130intergenic (+115/+200)yhiM/yhiNacid resistance protein, inner membrane/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (52/69);  new base T (5/3);  total (58/72)
Fisher's exact test for biased strand distribution p-value = 4.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 132 total aligned reads displayed.

TTTCAATATTAGAAGCGGGTTCTGCTAAAAAATAATTGCAACGTACCGGATAAAACCAGCGTTGACCATTTGCGTAACGCTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGCATGGAGGGTTTCAGTTCACGGGCTCATTAGAAAATAATCACAAGATAACCACTATC  >  NC_000913/3635859‑3636153
                                                                                                                                                     |                                                                                                                                                  
tttCAATATTAGAAGCGGGTTCTGCTAAAAAATAATTGCAACGTACCGGATAAAACCCGCGTTGACCATTTGCGTAACGCTGGTTTTTCTTAGGGATGATGATATAACGCCGCATTTATGCATAGTGGTTAAGTATAAAAAACCGCAAAg                                                                                                                                                    >  2:227348/1‑150 (MQ=255)
tttCAATATTAGAAGCGGGTTCTGCTAAAAAATAATTGCAACGTACCGGATAAAACCAGCGTTGACCATTTGCGTAACGCTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAg                                                                                                                                                    <  1:1590839/150‑1 (MQ=255)
  tCAATATTAGAAGCGGGTTCTGCTAAAAAATAATTGCAACGTACCGGATAAAACCAGCGTTGACCATTTGCGTAACGCTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGtt                                                                                                                                                  <  2:1281980/150‑1 (MQ=255)
  tCAATATTAGAAGCGGGTTCTGCTAAAAAATAATTGCAACGTACCGGATAAAACCAGCGTTGACCATTTGCGTAACGCTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGtt                                                                                                                                                  <  1:1606589/150‑1 (MQ=255)
   cAATATTAGAAGCGGGTTCTGCTAAAAAATAATTGCAACGTACCGGATAAAACCAGCGTTGACCATTTGCGTAACTCTGTTTTTTCTTAGGCATCATTAAATAACGCAGAATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAATTTa                                                                                                                                                 >  2:1326399/1‑150 (MQ=255)
     atatTAGAAGCGGGTTCTGCTAAAAAATAATTGCAACGTACCGGATAAAACCAGCGTTGACAATTTGCGTAACGCTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACt                                                                                                                                               >  1:787441/1‑150 (MQ=255)
         tAGAAGCGGGTTCTGCTAAAAAATAATTGCAACGTACCGGATAAAACCAGCGTTGACCATTTGCGTAACGCTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGttt                                                                                                                                          >  1:615236/1‑150 (MQ=255)
           gtaGCGGGTTCTGCTAAAAAATAATTTCAACGTAACGGATAAAACCAGCGTTGACCATTTGCGTAACGCTGGTTTTTCTTATGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGttttt                                                                                                                                        <  2:825648/148‑1 (MQ=255)
           gAAGCGGGTTCTGCTAAAAAATAATTGCAACGTACCGGATAAAACCAGCGTTGACCATTTGCGTAACGCTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGttttt                                                                                                                                        >  2:1705836/1‑150 (MQ=255)
             aGCGGGTTCTGCTAAAAAATAATTGCAACGTACCGGATAAAACCAGCGTTGACCATTTGCGTAACGCTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGTAACTTTGCTGtttttta                                                                                                                                      <  1:188510/150‑2 (MQ=255)
                  gTTCTGCTAAAAAATAATTGCAACGTACCGGATAAAACCAGCGTTGACCATTTGCGTAACGCTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAAcc                                                                                                                                 <  1:1823604/150‑1 (MQ=255)
                       gCTAAAAAATAATTGCAACGTACCGGATAAAACCAGCGTTGACCATTTGCGTAACGCTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTc                                                                                                                            >  1:728174/1‑150 (MQ=255)
                       gCTAAAAAATAATTGCAACGTACCGGATAAAACCAGCGTTGACCATTTGCGTAACGCTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTc                                                                                                                            >  2:2081068/1‑150 (MQ=255)
                        cTAAAAAATAATTGCAACGTACCGGATAAAACCAGCGTTGACCATTTGCGTAACGCTGGTTTTTCTTAGGCATCATGAAATAACGAATCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTATCAACCTGTTCa                                                                                                                           >  2:1105773/1‑150 (MQ=255)
                           aaaaaTAATTGCAACGTACCGGATAAAACCAGCGTTGACCATTTGCGTAACGCTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCatat                                                                                                                        >  2:1381271/1‑150 (MQ=255)
                              aataatTGCAACGTACCGGATAAAACCAGCGTTGACCATTTGCGTAACGCTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTc                                                                                                                     >  1:1674347/1‑150 (MQ=255)
                               ataatTGCAACGTACCGGATAAAACCAGCGTTGACCATTTGCGTAACGCTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCa                                                                                                                    <  1:1838015/150‑1 (MQ=255)
                               ataatTGCAACGTACAGGATAAAACCAGCGTTGACCATTTGCGTAACGCTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCTTAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCa                                                                                                                    <  1:1367799/150‑1 (MQ=255)
                                 aatTGCAACGTACCGGATAAAACCAGCGTTGACCATTTGCGTAACGCTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATa                                                                                                                  <  1:1704223/150‑1 (MQ=255)
                                 aatTGCAACGTACCGGATAAAACCAGCGTTGACCATTTGCGTAACGCTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATa                                                                                                                  <  1:134203/150‑1 (MQ=255)
                                  atTGCAACGTACCGGATAAAACCAGCGTTGACCATTTGCGTAACGCTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATaa                                                                                                                 <  2:1013363/150‑1 (MQ=255)
                                      cAACGTACCGGATAAAACCAGCGTTGACCATTTGCGTAACGCTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGAt                                                                                                             >  1:2204959/1‑150 (MQ=255)
                                       aaCGTACCGGATAAAACCAGCGTTGACCATTTGCGTAACGCTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATc                                                                                                            <  1:1960740/150‑1 (MQ=255)
                                       aaCGTACCGGATAAAACCAGCGTTGACCATTTGCGTAACGCTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATc                                                                                                            <  1:650198/150‑1 (MQ=255)
                                       aaCGTACCGGATAAAACCAGCGTTGACCATTTGCGTAACGCTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATc                                                                                                            <  1:903403/150‑1 (MQ=255)
                                           tACCGGATAAAACCAGCGTTGACCATTTGCGTAACGCTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGc                                                                                                        >  1:164551/1‑150 (MQ=255)
                                           tACCGGATAAAACCAGCGTTGACCATTTGCGTAACGCTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTAATAAAGATCTGGc                                                                                                        >  1:2214395/1‑150 (MQ=255)
                                             ccGGATAAAACCAGCGTTGACCATTTGCGTAACGCTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCa                                                                                                      >  1:721369/1‑150 (MQ=255)
                                              cGGATAAAACCAGCGTTGACCATTTGCGTATCGCTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCAc                                                                                                     <  2:1689873/150‑1 (MQ=255)
                                              cGGATAAAACCAGCGTTGACCATTTGCGTAACGCTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCAc                                                                                                     <  1:893344/150‑1 (MQ=255)
                                               ggATAAAACCAGCGTTGACCATTTGCGTAACGCTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACt                                                                                                    >  1:687268/1‑150 (MQ=255)
                                               ggATAAAACCAGAGTTGACCATTTGCGTAACTCTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACt                                                                                                    <  2:180813/150‑1 (MQ=255)
                                                   aaaaCCAGCGCTGACCATTTGCGTAACGCTGGTTTTTCTTAGTCATCATTAAATTACGCAGCATTAATGCATAGTGTTTAAGTATCAAACACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACtgagt                                                                                                <  2:1141215/150‑1 (MQ=255)
                                                    aaaCCAGCGTTGACCATTTGCGTAACGCTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACtgagtg                                                                                               >  1:64529/1‑150 (MQ=255)
                                                    aaaCCAGCGTTGACCATTTGCGTAACGCTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACtgagtg                                                                                               >  1:1056739/1‑150 (MQ=255)
                                                      aCCAGCGTTGACCATTTGCGTAACGCTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACtgagtgag                                                                                             >  2:947892/1‑150 (MQ=255)
                                                         aGCGTTGACCATTTGCGTAACGCTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGttt                                                                                          <  2:685956/150‑1 (MQ=255)
                                                         aGCGTTGACCATTTGCGTAACGCTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGttt                                                                                          <  2:120187/150‑1 (MQ=255)
                                                            tttGAACATTTGAGTAACGCTGGTTTTTATTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGTCCACTGAGTGAGTTTTCa                                                                                       <  2:212532/149‑1 (MQ=255)
                                                            gTTGACCTTTTGCGTAACGCTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCa                                                                                       <  1:833437/150‑1 (MQ=255)
                                                            gTTGACCATTTGCGTAACGCTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCa                                                                                       <  2:205621/150‑1 (MQ=255)
                                                              tGACCATTTGCGTAACGCTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTAATAAAGATCTGTCCACTGAGTGAGTTTTCAAt                                                                                     >  1:1561314/1‑150 (MQ=255)
                                                               gACCATTTGCGTAACGCTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATc                                                                                    <  2:915380/150‑1 (MQ=255)
                                                               gACCATTTGCGTAACGCTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATc                                                                                    <  2:964757/150‑1 (MQ=255)
                                                                      tGCGTAACGCTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTTGTTAAGTATAAAAAACAGCAAATTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGGTTTCAATCACCTTtc                                                                             <  2:1504555/150‑1 (MQ=255)
                                                                      tGCGTAACGCTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTtc                                                                             <  1:2121232/150‑1 (MQ=255)
                                                                                tGGTTTTTCTTAGGCATCATGAAATTACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAACGGTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCtt                                                                   <  1:130913/150‑1 (MQ=255)
                                                                                 ggTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGACACTGAGTGAGTTTTCAATCACCTTTCCATCAACCtt                                                                   >  1:1868245/1‑150 (MQ=255)
                                                                                 ggTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTta                                                                  >  1:1105102/1‑150 (MQ=255)
                                                                                         ttAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGc                                                          <  1:1453162/150‑1 (MQ=255)
                                                                                         ttAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGc                                                          <  2:2084752/150‑1 (MQ=255)
                                                                                         ttAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGc                                                          <  2:1389271/150‑1 (MQ=255)
                                                                                          tAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGCa                                                         >  1:531404/1‑150 (MQ=255)
                                                                                          tAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGCa                                                         <  2:687268/150‑1 (MQ=255)
                                                                                            ggCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGCATg                                                       <  2:2038530/150‑1 (MQ=255)
                                                                                            ggCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGCATg                                                       <  1:1705836/150‑1 (MQ=255)
                                                                                             gCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGCATgg                                                      <  1:2081068/150‑1 (MQ=255)
                                                                                               atcatGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGCATGGAg                                                    <  1:516340/150‑1 (MQ=255)
                                                                                               atcatGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGCATGGAg                                                    >  1:313925/1‑150 (MQ=255)
                                                                                                  atGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGCATGGAGGGt                                                 >  2:2193190/1‑150 (MQ=255)
                                                                                                  atGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCAATCCACCTTATATTAAGCATGGAGGGt                                                 >  1:1561155/1‑150 (MQ=255)
                                                                                                   tGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGCATGGAGGGtt                                                >  2:1099227/1‑150 (MQ=255)
                                                                                                   tGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGCATGGAGGGtt                                                >  2:1855045/1‑150 (MQ=255)
                                                                                                   tGAAATAACGCAGCATTAATGAATAGTGGTTAAGTAAAAAATACAGAAAACTTACTGTATTTTTCAAACTGTTCATATTTCATAAAGATCTGTCCACAGACTGAGTTTTCAATCACCTTTCCAACCACCTTATATTAACCAATGAgggat                                                >  1:2179057/1‑148 (MQ=255)
                                                                                                     aaaTAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGCATGGAGGGTTTc                                              <  2:654820/150‑1 (MQ=255)
                                                                                                     aaaTAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGCATGGAGGGTTTc                                              >  1:933236/1‑150 (MQ=255)
                                                                                                      aaTAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGCATGGAGGGTTTCa                                             >  1:1241652/1‑150 (MQ=255)
                                                                                                         aaCGCATCATTAATGCATAGTGGTTAAGTATAAAAAACATCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCATTCCATCCACCTTATATTAAGCATGGAGGGTTTCAGtt                                          <  1:375443/150‑1 (MQ=255)
                                                                                                           cGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGCATGGAGGGTTTCAGTTCa                                        >  2:1432610/1‑150 (MQ=255)
                                                                                                            gcagcaTTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGCATGGAGGGTTTCAGTTCAc                                       <  1:1918111/150‑1 (MQ=255)
                                                                                                             aagaaTTAATGCATATTGGTTAATTATAAAAAACAGCAAATTTACTGTTTTTTTCAACCTGTTAATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTAAATCACCTTTCCATCCACCTTATATTAAGCATGGAGGGTTTCAGTTCACg                                      <  1:30213/146‑1 (MQ=255)
                                                                                                               gcaTTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGCATGGAGGGTTTCAGTTCACggg                                    >  1:883869/1‑150 (MQ=255)
                                                                                                                caTTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGCATGGAGGGTTTCAGTTCACGGGc                                   <  2:1838940/150‑1 (MQ=255)
                                                                                                                     aTGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGCATGGAGGGTTTCAGTTCACGGGCTCAtt                              <  1:1045867/150‑1 (MQ=255)
                                                                                                                     aTGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTGTTCAATCACCTTTCCATCCACCTTATATTAAGCATGGAGGGTTTCAGTTCACGGGCTCAtt                              <  2:2006017/150‑1 (MQ=255)
                                                                                                                      tGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGCATGGAGGGTTTCAGTTCACGGGCTCATTa                             <  1:339643/150‑1 (MQ=255)
                                                                                                                      tGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGCATGGAGGGTTTCAGTTCACGGGCTCATTa                             <  2:1056739/150‑1 (MQ=255)
                                                                                                                       gCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGCATGTAGGGTTTCAGTTCACGGGATCATTat                            >  2:208694/1‑149 (MQ=255)
                                                                                                                              ggTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGCATGGAGGGTTTCAGTTCACGGGCTCATTAGAAaataa                     >  2:1601751/1‑150 (MQ=255)
                                                                                                                                ttAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATATCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGCATGGAGGGTTTCAGTTCACGGGCTCATTAGAAAATAATc                   <  1:1066521/150‑1 (MQ=255)
                                                                                                                                 tAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTTAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGCATGGAGGGTTTCAGTTCACGGGCTCATTAGAAAATAATca                  <  1:1381271/150‑1 (MQ=255)
                                                                                                                                  ggttgaaaaaaaCAGTAACTTTGCTGTTTTTTTCAACATGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCAACTTATATTAAGAATGGAGGGTTTCAGTTCACGGTCTCATTAGAAAATAATcaa                 >  2:1479745/7‑149 (MQ=255)
                                                                                                                                   gttgaaaaaaaCAGTAACTTTGCTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGCATGGAGGGTTTCAGTTCACGGGCTCATTAGAAAATAATcaca                >  1:1452360/6‑150 (MQ=255)
                                                                                                                                   aGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGCATGGAGGGTTTCAGTTCACGGGCTCATTAGAAAATAATcaca                <  2:1674347/150‑1 (MQ=255)
                                                                                                                                   aGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGCATGGAGGGTTTCAGTTCACGGGCTCATTAGAAAATAATcaca                <  1:1306907/150‑1 (MQ=255)
                                                                                                                                    ttgaaaaaaaCAGTAACTTTGCTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGCATGGAGGGTTTCAGTTCACGGGCTCATTAGAAAATAATCACaa               >  1:1749606/5‑150 (MQ=255)
                                                                                                                                    gTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGCATGGAGGGTTTCAGTTCACGGGCTCATTAGAAAATAATCACaa               >  2:788503/1‑150 (MQ=255)
                                                                                                                                    gTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGGTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGCATGGAGGGTTTCAGTTCACGGGCTCATTAGAAAATAATCACaa               <  2:1637568/150‑1 (MQ=255)
                                                                                                                                     tataAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGCATGGAGGGTTTCAGTTCACGGGCTCATTAGAAAATAATCACAAg              <  2:1496193/150‑1 (MQ=255)
                                                                                                                                     tataAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTAAATTAAGCATGGAGGGTTTCAGTTCACGGGCTCATTAGAAAATAATCACAAg              <  1:1461499/150‑1 (MQ=255)
                                                                                                                                     tataAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAAACACCTTTCCATCCACCTTATATTAAGCATGGAGGGTTTCAGTTCACGGGCTCATTAGAAAATAATCACAAg              >  2:1047738/1‑150 (MQ=255)
                                                                                                                                       taAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGCATGGAGGGTTTCAGTTCACGGGCTCATTAGAAAATAATCACAAGAt            <  2:443129/150‑1 (MQ=255)
                                                                                                                                       taAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGCATGGAGGGTTTCAGTTCACGGGCTCATTAGAAAATAATCACAAGAt            >  2:740940/1‑150 (MQ=255)
                                                                                                                                       aaaaaaaCAGTAACTTTGCTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGCATGGAGGGTTTCAGTTCACGGGCTCATTAGAAAATAATCACAAGAt            >  2:1902121/2‑150 (MQ=255)
                                                                                                                                        aaaaaaCAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGCATGGAGGGTTTCAGTTCACGGGCTCATTAGAAAATAATCACAAGATa           >  2:346778/1‑150 (MQ=255)
                                                                                                                                        aaaaaaCAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGCATGGAGGGTTTCAGTTCACGGGCTCATTAGAAAATAATCACAAGATa           >  2:1586553/1‑150 (MQ=255)
                                                                                                                                         aaaaaCAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGCATGGAGGGTTTCAGTTCACGGGCTCATTAGAAAATAATCACAAGATaa          >  1:745539/1‑150 (MQ=255)
                                                                                                                                             aCAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGCATGGAGGGTTTCAGTTCACGGGCTCATTAGAAAATAATCACAAGATAACCAc      <  2:494078/150‑1 (MQ=255)
                                                                                                                                                gCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGCATGGAGGGTTTCAGTTCACGGGCTCATTAGAAAATAATCACAAGATAACCACTAt   <  1:1586553/150‑1 (MQ=255)
                                                                                                                                                 cAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGCATGGAGGGTTTCAGTTCACGGGCTCATTAGAAAATAATCACAAGATAACCACTATc  <  1:240422/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
TTTCAATATTAGAAGCGGGTTCTGCTAAAAAATAATTGCAACGTACCGGATAAAACCAGCGTTGACCATTTGCGTAACGCTGGTTTTTCTTAGGCATCATGAAATAACGCAGCATTAATGCATAGTGGTTAAGTATAAAAAACAGCAAAGTTACTGTTTTTTTCAACCTGTTCATATTTCATAAAGATCTGGCCACTGAGTGAGTTTTCAATCACCTTTCCATCCACCTTATATTAAGCATGGAGGGTTTCAGTTCACGGGCTCATTAGAAAATAATCACAAGATAACCACTATC  >  NC_000913/3635859‑3636153

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: