Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,657,603 T→A 5.3% intergenic (+36/+383) hdeD → / ← arrS acid‑resistance membrane protein/antisense sRNA ArrS, function unknown

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,657,6030TA 5.3% 486.0 / 11.4 160intergenic (+36/+383)hdeD/arrSacid‑resistance membrane protein/antisense sRNA ArrS, function unknown
Reads supporting (aligned to +/- strand):  ref base T (82/69);  new base A (6/3);  total (88/72)
Fisher's exact test for biased strand distribution p-value = 5.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

Only 100 of 165 total aligned reads displayed.

GGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCAAATTAGCCATTTCAA  >  NC_000913/3657454‑3657752
                                                                                                                                                     |                                                                                                                                                     
ggTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGAt                                                                                                                                                       <  2:410255/150‑1 (MQ=255)
 gTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGAtg                                                                                                                                                      >  2:1162628/1‑150 (MQ=255)
 gTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGAtg                                                                                                                                                      >  2:1454944/1‑150 (MQ=255)
     aaCGCCGATGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGAc                                                                                                                                                  <  1:1311230/150‑1 (MQ=255)
        gCCGATGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTAt                                                                                                                                               >  1:596121/1‑150 (MQ=255)
         ccGATGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATg                                                                                                                                              <  2:2155772/150‑1 (MQ=255)
          cGATGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGa                                                                                                                                             <  2:911441/150‑1 (MQ=255)
               gtgtCTGTAACGCTGGTTTCGACACTGGTGGGAATTGAACTGATATTTAGCGCAGCCAGCCTGTTCAGCTGCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACATCAAGTGACTATGATCCgg                                                                                                                                        <  1:1761946/150‑2 (MQ=255)
               gtgtCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGa                                                                                                                                        >  1:815025/1‑150 (MQ=255)
               gtgtCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACATCAAGTGACTATGATCCgg                                                                                                                                        <  1:1863395/150‑2 (MQ=255)
                tgtCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACATCAAGTGACTATGATCCggg                                                                                                                                       <  1:630371/150‑3 (MQ=255)
                 gtCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGAtt                                                                                                                                      <  2:742894/150‑1 (MQ=255)
                     gTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATa                                                                                                                                  >  2:1845720/1‑150 (MQ=255)
                      tAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATAc                                                                                                                                 <  1:723372/150‑1 (MQ=255)
                        aCGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATActc                                                                                                                               <  1:1745624/150‑1 (MQ=255)
                        aCGCTGGCTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATActc                                                                                                                               <  2:138598/150‑1 (MQ=255)
                         cGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCCGCTTCGCGAGTTTGGTCGTTAAGCAGCAATAATTACCACGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTTTTActct                                                                                                                              >  1:342237/1‑150 (MQ=255)
                              gTTTCCACACTGGTGGGAATTGAACTGATATTTAGAGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCg                                                                                                                         >  2:2146375/1‑150 (MQ=255)
                                   cacaCTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACg                                                                                                                    >  2:1705981/1‑150 (MQ=255)
                                   cacaCTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACg                                                                                                                    >  1:1031017/1‑150 (MQ=255)
                                    acacTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGc                                                                                                                   <  1:1520629/150‑1 (MQ=255)
                                        tggtggGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAgtg                                                                                                               <  1:312731/150‑1 (MQ=255)
                                         ggtggGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCCGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAgggt                                                                                                              >  2:872137/1‑150 (MQ=255)
                                          gtggGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGtgtt                                                                                                             >  2:575727/1‑150 (MQ=255)
                                          gtggGAATTGAACTGATATTTAGAGCCGCCATCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGGAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGtgtt                                                                                                             <  2:1552723/150‑1 (MQ=255)
                                           ttggAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGtgttg                                                                                                            <  1:1176168/148‑1 (MQ=255)
                                           tggGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCTGTTTGTTCGTTAAGCTGCTATATTTACCCCGGTTGTCACCCGGTTCATAGTCACTTGATGTGTGTATGATCCGATTAATTCTCTCTCCGCTACGCTGggttg                                                                                                            >  1:2082858/1‑150 (MQ=255)
                                            gggAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGtgttgt                                                                                                           >  1:1823855/1‑150 (MQ=255)
                                              taaTTTAACTCATATTTAGCCCCGCCCGCCGGTTCAGCTTCCCCCGTTTGTTCTTTAAGCAGAAAAAATTACCCCGGTTGTCACCCGGACCATAGCCACTTCATGTCACTATGATCCGATTAATACTCTCTCCCCACCGCCGTGTTGTac                                                                                                         <  2:1798963/149‑2 (MQ=255)
                                              gAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAg                                                                                                         >  2:22044/1‑150 (MQ=255)
                                              gAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAAGAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAg                                                                                                         >  1:2065196/1‑150 (MQ=255)
                                              caaTTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAg                                                                                                         >  2:875578/2‑150 (MQ=255)
                                                 ttGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATTCTCTCTCCGCTACGCAGTGGTGTAGATc                                                                                                      >  2:1949473/1‑150 (MQ=255)
                                                 ttGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATc                                                                                                      >  1:6829/1‑150 (MQ=255)
                                                   gAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCaa                                                                                                    >  2:1824519/1‑150 (MQ=255)
                                                   gAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCaa                                                                                                    <  2:480006/150‑1 (MQ=255)
                                                        gATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTgcg                                                                                               >  1:2113665/1‑150 (MQ=255)
                                                        gATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTgcg                                                                                               >  2:1748556/1‑150 (MQ=255)
                                                        gATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTgcg                                                                                               <  1:478673/150‑1 (MQ=255)
                                                         atatTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTgcgc                                                                                              >  2:784323/1‑150 (MQ=255)
                                                         atatTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTgcgc                                                                                              <  2:2175715/150‑1 (MQ=255)
                                                           atTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCAc                                                                                            >  2:148410/1‑150 (MQ=255)
                                                           atTTAGCGCCGCAAGCCTGTTAATCTTCGCCAGTTTGTTCTTTAAGCATCAATAATTACCACGGTTGTCACCCTGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCAc                                                                                            <  1:1054853/150‑1 (MQ=255)
                                                            tttAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGAAGTGTTGTAGATCAATTGCGCACt                                                                                           >  1:1712242/1‑150 (MQ=255)
                                                               aGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATc                                                                                        >  2:637444/1‑150 (MQ=255)
                                                                gcgcCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCa                                                                                       >  1:424035/1‑150 (MQ=255)
                                                                 cgccgcCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCAt                                                                                      <  1:1554412/150‑1 (MQ=255)
                                                                  gccgccAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCAGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCAtt                                                                                     >  1:1723895/1‑150 (MQ=255)
                                                                  gccgccAGCCTGTTCAGCTTCGCAAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTAGTCACCCGAATCATAGTCACTTGATGAGACTATGATCCGATTTATACTCTCTCCGCTACGCAGTGTTGTAGATCAATAGCGCACTATCAtt                                                                                     >  1:330102/1‑150 (MQ=255)
                                                                   ccgccAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTg                                                                                    <  1:575956/150‑1 (MQ=255)
                                                                    cgccAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGGAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGa                                                                                   <  2:1670266/150‑1 (MQ=255)
                                                                     gccAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCTGCAATAATTACCCCGGTTGTCACCCGGATGATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTTTTGTAGATCAATTGCGCACTATCATTGaa                                                                                  <  2:822234/150‑1 (MQ=255)
                                                                     gccAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGaa                                                                                  >  2:941370/1‑150 (MQ=255)
                                                                       cAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGAGTTGTAGATCAATTGCGCACTATCATTGAaat                                                                                >  1:1390618/1‑150 (MQ=255)
                                                                           cTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAAtt                                                                            <  2:1952288/150‑1 (MQ=255)
                                                                            tGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTa                                                                           >  2:1194875/1‑150 (MQ=255)
                                                                                 aGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACATGc                                                                      >  1:405743/1‑150 (MQ=255)
                                                                                        ccAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGat                                                               >  1:1492803/1‑150 (MQ=255)
                                                                                             ttGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTAttt                                                          <  1:643061/150‑1 (MQ=255)
                                                                                                 tCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAAc                                                      <  1:1781083/150‑1 (MQ=255)
                                                                                                  cGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAAcc                                                     <  1:1948090/150‑1 (MQ=255)
                                                                                                  cGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAAcc                                                     <  2:1723895/150‑1 (MQ=255)
                                                                                                   gTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCt                                                    >  1:2176235/1‑150 (MQ=255)
                                                                                                      aaGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACt                                                 >  1:888728/1‑150 (MQ=255)
                                                                                                      aaGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACt                                                 <  2:1776113/150‑1 (MQ=255)
                                                                                                      aaGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACt                                                 >  2:1146701/1‑150 (MQ=255)
                                                                                                             aataatTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTc                                          >  1:1484260/1‑150 (MQ=255)
                                                                                                              ataatTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGAACTATCATTGAAATAATTAACTGCTAGTCATTATTTCAACCTAATGAATTTCa                                         >  1:556665/1‑150 (MQ=255)
                                                                                                              ataatTACACCTGTTGTCAACCGGATCATAGTCACTTGATGTGACTATGATCAGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTAACTGCTAGTGATTATTTCAACCTACTGAATTTCa                                         <  1:460217/150‑1 (MQ=255)
                                                                                                               taatTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCAt                                        <  1:1845720/150‑1 (MQ=255)
                                                                                                                       ccGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTAAGCAGTGTTTTAGATCAATTGCTCACTATCATTGAAAAAATTACCAGCTAGTTATTATTTCATCCTACTGAATTTAATATAAtttt                                >  1:141731/1‑150 (MQ=255)
                                                                                                                         ggTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAAtttttt                              <  1:1676659/150‑1 (MQ=255)
                                                                                                                          gTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAAttttttt                             <  1:2146375/150‑1 (MQ=255)
                                                                                                                          gTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAAttttttt                             <  2:2080603/150‑1 (MQ=255)
                                                                                                                          gTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAAttttttt                             <  2:1623192/150‑1 (MQ=255)
                                                                                                                          gTTGTCACCCGGATCATAGTCACTTGATGGGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAAttttttt                             <  1:1454944/150‑1 (MQ=255)
                                                                                                                           ttGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTc                            <  1:921474/150‑1 (MQ=255)
                                                                                                                           ttGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTc                            <  2:6829/150‑1 (MQ=255)
                                                                                                                            tGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCa                           >  2:428728/1‑150 (MQ=255)
                                                                                                                             gTCACCAGGATCATAGTCACTTGATGTGACTATGATACGATTAATACTCTCTCCGCAACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCAc                          <  1:1368226/150‑1 (MQ=255)
                                                                                                                               cACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCActc                        <  1:2095987/150‑1 (MQ=255)
                                                                                                                               cACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCActc                        >  1:468028/1‑150 (MQ=255)
                                                                                                                               cACCCGGATAATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGATACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCActc                        <  2:363430/150‑1 (MQ=255)
                                                                                                                                aCCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCActct                       >  1:735738/1‑150 (MQ=255)
                                                                                                                                  ccGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTAt                     <  1:2197834/150‑1 (MQ=255)
                                                                                                                                   cGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATg                    <  1:1146701/150‑1 (MQ=255)
                                                                                                                                     gATCATAGTCACATCAAGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGc                  >  2:1337445/1‑150 (MQ=255)
                                                                                                                                     gATCATAGTCACATCAAGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGc                  >  1:1535533/1‑150 (MQ=255)
                                                                                                                                        cATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCaaa               >  2:288462/1‑150 (MQ=255)
                                                                                                                                        cATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATATAATTTTTTTCACTCTATGGCaaa               >  1:431638/1‑150 (MQ=255)
                                                                                                                                         aTAGTCACATCAAGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCAAAt              >  1:1323932/1‑150 (MQ=255)
                                                                                                                                          tAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCAAAtt             <  1:1897557/150‑1 (MQ=255)
                                                                                                                                           aGTCACTTGATGTGACTATGCTCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCTATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCAAATTa            >  1:366076/1‑150 (MQ=255)
                                                                                                                                           aGTCACATCAAGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCAAATTa            >  1:247899/1‑150 (MQ=255)
                                                                                                                                            gTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCAAATTAg           <  1:1703231/150‑1 (MQ=255)
                                                                                                                                             tCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCAAATTAGc          <  1:1194875/150‑1 (MQ=255)
                                                                                                                                              cACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCAAATTAGcc         >  1:163587/1‑150 (MQ=255)
                                                                                                                                              cACTTGATGTGACTAGGATCCGATTAATACACTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATGTCATCTAATTTTTTTCACTCTATGGCAAATTAGcc         >  2:1138802/1‑150 (MQ=255)
                                                                                                                                                   taTGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGTTTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCAAATTAGCCATTTc    <  2:506331/149‑1 (MQ=255)
                                                                                                                                                     tgtgACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCTAATTTGCCAtagagt  <  1:637444/150‑6 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GGTGCAACGCCGATGGTGTCTGTAACGCTGGTTTCCACACTGGTGGGAATTGAACTGATATTTAGCGCCGCCAGCCTGTTCAGCTTCGCCAGTTTGTTCGTTAAGCAGCAATAATTACCCCGGTTGTCACCCGGATCATAGTCACTTGATGTGACTATGATCCGATTAATACTCTCTCCGCTACGCAGTGTTGTAGATCAATTGCGCACTATCATTGAAATAATTACCTGCTAGTGATTATTTCAACCTACTGAATTTCATCTAATTTTTTTCACTCTATGGCAAATTAGCCATTTCAA  >  NC_000913/3657454‑3657752

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: