Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,663,556 A→C 6.6% intergenic (+29/+334) mdtF → / ← gadW anaerobic multidrug efflux transporter, ArcA‑regulated/transcriptional activator of gadA and gadBC; repressor of gadX

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,663,5560AC 6.6% 462.0 / 18.6 152intergenic (+29/+334)mdtF/gadWanaerobic multidrug efflux transporter, ArcA‑regulated/transcriptional activator of gadA and gadBC; repressor of gadX
Reads supporting (aligned to +/- strand):  ref base A (74/68);  new base C (7/3);  total (81/71)
Fisher's exact test for biased strand distribution p-value = 3.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.43e-01

Only 100 of 156 total aligned reads displayed.

AAACGCGGTAGGTACCGGCGTGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTGAACAACACAATACTGACCCACATTC  >  NC_000913/3663407‑3663705
                                                                                                                                                     |                                                                                                                                                     
aaaCGCGGTAGGTACCGGCGTGATGGGCGGGATGGTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGa                                                                                                                                                       <  2:1828992/150‑1 (MQ=255)
 aaCGCGGTAGGTACCGGCGTGATGGGAGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGACCATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGaa                                                                                                                                                      >  1:609499/1‑150 (MQ=255)
         aggtaCCGGCGTGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGGCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGc                                                                                                                                              <  2:224716/150‑1 (MQ=255)
          ggtaCCGGCGTGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGcc                                                                                                                                             >  2:1436008/1‑150 (MQ=255)
            taCCGGCGTGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCtt                                                                                                                                           >  1:2099396/1‑150 (MQ=255)
              ccGGCGTGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTAc                                                                                                                                         >  1:427305/1‑150 (MQ=255)
                ggCGTGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTActc                                                                                                                                       <  1:934326/150‑1 (MQ=255)
                  cGTGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCtt                                                                                                                                     >  2:1766768/1‑150 (MQ=255)
                   gTGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCttt                                                                                                                                    >  1:2177904/1‑150 (MQ=255)
                      cggggcgggcGGCTTGCCGCAACAGTGCTGGCAATTTACTTCTGTCCGGTCATTTTCGTTGTAGTGGACCATCTCTTTGCCCGCTTTAAAAAACCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCt                                                                                                                                 <  2:2081132/148‑1 (MQ=255)
                       tGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACTTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTg                                                                                                                                >  2:332974/1‑150 (MQ=255)
                            gggATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAAc                                                                                                                           <  1:498871/150‑1 (MQ=255)
                                tGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCAtt                                                                                                                       <  1:1983405/150‑1 (MQ=255)
                                 gTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTc                                                                                                                      >  2:1036241/1‑150 (MQ=255)
                                 gTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTc                                                                                                                      <  2:1975750/150‑1 (MQ=255)
                                    tGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTcacc                                                                                                                   <  2:1163979/150‑1 (MQ=255)
                                     gCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTcacca                                                                                                                  >  1:1028084/1‑150 (MQ=255)
                                          aaCAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTcaa                                                                                                             <  2:167949/150‑1 (MQ=255)
                                          aaCAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTcaa                                                                                                             <  2:411879/150‑1 (MQ=255)
                                          aaCAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTcaa                                                                                                             >  1:970670/1‑150 (MQ=255)
                                           aCAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTcaac                                                                                                            <  2:1902931/150‑1 (MQ=255)
                                              gTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCaacaac                                                                                                         <  2:886660/150‑1 (MQ=255)
                                              gTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCaacaac                                                                                                         >  2:2032932/1‑150 (MQ=255)
                                               tGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAAcc                                                                                                        <  2:442234/150‑1 (MQ=255)
                                                gCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGACCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCa                                                                                                       <  1:671320/150‑1 (MQ=255)
                                                 cTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAg                                                                                                      <  1:1002793/150‑1 (MQ=255)
                                                    gCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAGATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTa                                                                                                   <  2:1078724/150‑1 (MQ=255)
                                                     cAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCCCCACTCAACAACCAGCTaa                                                                                                  >  1:1861340/1‑150 (MQ=255)
                                                        tttACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCg                                                                                               >  2:123352/1‑150 (MQ=255)
                                                          tACTTCGTTCCGGTCTTTTTCGGTGTAGTGGAACATCTCTTTGCCCGCGTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCGTTACTCTGTCCGGCTAACCATTCCCCACTCCACAGCCAGCTAACCGTc                                                                                             >  2:2072937/1‑150 (MQ=255)
                                                             ttCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCTTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACCACCAGCTCACCGTCAgg                                                                                          >  1:364400/1‑150 (MQ=255)
                                                             ttCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAgg                                                                                          <  1:410350/150‑1 (MQ=255)
                                                              tCGTTCCTGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGATGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGa                                                                                         <  1:2123520/150‑1 (MQ=255)
                                                              tCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTATGCCCGCTTTAAAAAAGCGTAACCTGTAAATGAGAGTAAGCTTGAACATGAAGCTTCAGCCTTACTCTTTCCTGCTAACCATTAACCACTCAACAAACAGCTAACCTTCAgca                                                                                         >  1:1974680/1‑148 (MQ=255)
                                                               cGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGaa                                                                                        >  2:2049257/1‑150 (MQ=255)
                                                                 ttCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATa                                                                                      >  2:904902/1‑150 (MQ=255)
                                                                   ccGGTATTTTTCGTTGTAGTGGAACATCTATTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATAcc                                                                                    <  2:373272/150‑1 (MQ=255)
                                                                    cGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACAATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCa                                                                                   >  1:308158/1‑150 (MQ=255)
                                                                        cTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCATCTAACCGTCAGGAATACCAGGCt                                                                               >  2:595887/1‑150 (MQ=255)
                                                                        cTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCt                                                                               >  1:1942354/1‑150 (MQ=255)
                                                                             tCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTa                                                                          <  2:96210/150‑1 (MQ=255)
                                                                             tCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTa                                                                          <  1:646899/150‑1 (MQ=255)
                                                                             tCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTa                                                                          <  1:1360614/150‑1 (MQ=255)
                                                                              cGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTac                                                                         >  1:2193982/1‑150 (MQ=255)
                                                                              cGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTac                                                                         <  1:353919/150‑1 (MQ=255)
                                                                                       ggAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTg                                                                >  1:2189063/1‑150 (MQ=255)
                                                                                       ggAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTg                                                                >  2:448579/1‑150 (MQ=255)
                                                                                       ggAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTg                                                                <  2:2199419/150‑1 (MQ=255)
                                                                                         aaCATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGGTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGaa                                                              <  1:1779934/150‑1 (MQ=255)
                                                                                           cATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACCCAGCACTGAAAg                                                            >  1:1127413/1‑150 (MQ=255)
                                                                                           cATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAg                                                            <  2:1449980/150‑1 (MQ=255)
                                                                                           cATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAg                                                            >  2:2032790/1‑150 (MQ=255)
                                                                                            aTCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGt                                                           <  2:767902/150‑1 (MQ=255)
                                                                                            aTCTCTTTGCCCGCTTTAAAAAAGCGTAACGCGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGACTTAGTACACAGCACTGAAAGt                                                           <  2:1095039/150‑1 (MQ=255)
                                                                                             tctcTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTa                                                          <  2:1044154/150‑1 (MQ=255)
                                                                                              ctctTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAg                                                         <  2:765612/150‑1 (MQ=255)
                                                                                               tctTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGa                                                        >  1:1098322/1‑150 (MQ=255)
                                                                                                ctTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGaa                                                       <  2:272708/150‑1 (MQ=255)
                                                                                                ctTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGaa                                                       <  1:723498/150‑1 (MQ=255)
                                                                                                ctTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGaa                                                       <  2:1561412/150‑1 (MQ=255)
                                                                                                   tGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAAcc                                                    >  1:2085721/1‑150 (MQ=255)
                                                                                                   tGCACGCTTTAAAAAATCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGGTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCATGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAAcc                                                    <  1:1567749/150‑1 (MQ=255)
                                                                                                    gCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAAccc                                                   >  1:382089/1‑149 (MQ=255)
                                                                                                         cTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTACTACACAGCACTGAAAGTAGAAACCACTCCa                                              >  1:1696381/1‑150 (MQ=255)
                                                                                                           ttAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCAATCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGc                                            <  1:1164697/150‑1 (MQ=255)
                                                                                                             aaaaaaGCGTAACGTGTAAATGAGAGTAAGGTTGAACAAGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCa                                          >  1:32841/1‑150 (MQ=255)
                                                                                                              aaaaaGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCAt                                         <  2:238321/150‑1 (MQ=255)
                                                                                                                 aaGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCg                                      <  2:2099396/150‑1 (MQ=255)
                                                                                                                    cGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGtca                                   <  1:1658411/150‑1 (MQ=255)
                                                                                                                     gTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACCGCACTGAAAGAAGAAACCACTCCAGCCATTCGtcat                                  >  2:1437737/1‑150 (MQ=255)
                                                                                                                      tAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGtcatc                                 >  1:689035/1‑150 (MQ=255)
                                                                                                                        aCGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCaa                               >  2:1628039/1‑150 (MQ=255)
                                                                                                                           tgtAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAAttt                            >  1:1502720/1‑150 (MQ=255)
                                                                                                                            gtAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTGGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTg                           <  2:133747/150‑1 (MQ=255)
                                                                                                                            gtAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTg                           <  2:427305/150‑1 (MQ=255)
                                                                                                                             tAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTGa                          <  2:920844/150‑1 (MQ=255)
                                                                                                                             tAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTGa                          <  2:1705294/150‑1 (MQ=255)
                                                                                                                             caggaaagagTAAGGCTGAACCTTCATGTTCAACCTTACTCTTTCCTGCTAACCATTCACCCCGCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACCCCGCACTGAAGGTAGCAACCACTCCAGCCATACGTCATCAATTTGa                          >  2:1583494/7‑150 (MQ=25)
                                                                                                                             caggaaagagTAAGGCTGAACCTTCATGTTCAACCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTGa                          >  1:814195/7‑150 (MQ=38)
                                                                                                                               ggaaagagTAAGGCTGATCCTTCATGTTCAACCTTACTCTTTCCTGCTAAACATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTGcac                        >  1:1112102/5‑150 (MQ=38)
                                                                                                                               ggaaagagTAAGGCTGAACCTTCATGTTCAACCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATACGTCATCAATTTGaac                        >  1:1844862/5‑150 (MQ=38)
                                                                                                                               aaTGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTGaac                        <  1:247802/150‑1 (MQ=255)
                                                                                                                               aaTGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTGaac                        <  2:1171658/150‑1 (MQ=255)
                                                                                                                               aaTGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTGaac                        <  2:1776077/150‑1 (MQ=255)
                                                                                                                               aaTGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGGCATCAATTTGaac                        >  1:1373710/1‑150 (MQ=255)
                                                                                                                                 tGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTGaacaa                      >  2:1623857/1‑150 (MQ=255)
                                                                                                                                 tGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCAGCTAACCATTCACCACTAAACAACAAGATAACCGTCAGGAATACCAGGCTTAGTACCCAGCACTGAAAGTAGACACCACTCCAGCCATTCGTCATCAATTTGaacaa                      >  2:109314/1‑150 (MQ=255)
                                                                                                                                  aagagTAAGGCTGATCCTTCATGTTCAACCTTACTCTTTCCTGCTAACAATACACCACTCAACACCCAGCTAACAGTCAGGAATACCAGGCTTAGTTCACAGCACTGAAAGAAGAAGCCACTCCCGCCATTCGTCATAAATTTGaacaac                     >  1:1826577/2‑150 (MQ=255)
                                                                                                                                  aagagTAAGGCTGAACCTTCATGTTCAACCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTGaacaac                     >  1:843121/2‑150 (MQ=39)
                                                                                                                                   agagTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCATACCATGCACCTCTCACCAACCAGCTAACCGTCAGGAATACCAGCCTTAGTACACAGCACAGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTGAacaaca                    <  2:355235/150‑1 (MQ=255)
                                                                                                                                    gagTAAGGCTGAACCTTCATGTTCAACCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTGAACAacac                   >  1:1270360/1‑150 (MQ=255)
                                                                                                                                      ttAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTAAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACAACTCCAGCCATTCGTCATCAATTTGAACAACACaa                 >  1:520806/2‑150 (MQ=255)
                                                                                                                                      gTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTGAACAACACaa                 >  1:2064638/1‑150 (MQ=255)
                                                                                                                                      gTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACAGAAAGTAGAAACCACTCCAGCCATTCGGCATCAATTTTAACAACACaa                 >  2:384713/1‑150 (MQ=255)
                                                                                                                                      gTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCAGCTAACAATTCAACACTCAACAACCAGATAACAGTCAGGAATACCAGGCTTAGTAAACAGAAATGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTGAACAGCACaa                 >  2:522093/1‑150 (MQ=255)
                                                                                                                                         aGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTGAACAACACAATAc              >  2:1918154/1‑150 (MQ=255)
                                                                                                                                          ggTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTGAACAACACAATACt             >  2:583467/1‑150 (MQ=255)
                                                                                                                                              gAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTGAACAACACAATACTGAcc         <  1:23831/150‑1 (MQ=255)
                                                                                                                                                   tGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTGAACAACACAATACTGACCCACAt    >  2:1865963/1‑150 (MQ=255)
                                                                                                                                                    gAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTACCCGTCAGGAATACCAGGCTTAGTACACAGCACTCAAAGTAGAAACCACTCCAGACAATCGTCATCAATTAGAACAACACAATACTGACCCACAtt   >  2:919134/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AAACGCGGTAGGTACCGGCGTGATGGGCGGGATGTTTGCCGCAACAGTGCTGGCAATTTACTTCGTTCCGGTCTTTTTCGTTGTAGTGGAACATCTCTTTGCCCGCTTTAAAAAAGCGTAACGTGTAAATGAGAGTAAGGTTGAACATGAAGGTTCAGCCTTACTCTTTCCTGCTAACCATTCACCACTCAACAACCAGCTAACCGTCAGGAATACCAGGCTTAGTACACAGCACTGAAAGTAGAAACCACTCCAGCCATTCGTCATCAATTTGAACAACACAATACTGACCCACATTC  >  NC_000913/3663407‑3663705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: