Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,710,635 G→T 6.2% intergenic (‑160/+164) eptB ← / ← yhjX KDO phosphoethanolamine transferase, Ca(2+)‑inducible/pyruvate‑inducible inner membrane protein, putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,710,6350GT 6.2% 348.6 / 5.2 132intergenic (‑160/+164)eptB/yhjXKDO phosphoethanolamine transferase, Ca(2+)‑inducible/pyruvate‑inducible inner membrane protein, putative transporter
Reads supporting (aligned to +/- strand):  ref base G (54/68);  new base T (8/2);  total (62/70)
Fisher's exact test for biased strand distribution p-value = 4.53e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 134 total aligned reads displayed.

CCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCATTGTTTATACATTTCGTTGATGCGTTTGATGCACACGGAAGCTGAAGCCCAGTAG  >  NC_000913/3710486‑3710784
                                                                                                                                                     |                                                                                                                                                     
ccTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAg                                                                                                                                                       <  2:1489771/150‑1 (MQ=255)
  tGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGca                                                                                                                                                     <  1:550677/150‑1 (MQ=255)
  tGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAACCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGca                                                                                                                                                     <  1:2169970/150‑1 (MQ=255)
     aCAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACaa                                                                                                                                                  >  1:1346219/1‑150 (MQ=255)
      cAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTATTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGAATGAAGCACaaa                                                                                                                                                 >  2:740088/1‑150 (MQ=255)
      cAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACaaa                                                                                                                                                 >  2:820460/1‑150 (MQ=255)
          cAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTa                                                                                                                                             <  1:1105303/150‑1 (MQ=255)
            ggTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACa                                                                                                                                           <  2:1707944/150‑1 (MQ=255)
               gATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACaaaa                                                                                                                                        >  1:2205659/1‑150 (MQ=255)
                  aaCACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAATATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATg                                                                                                                                     <  2:1594244/150‑1 (MQ=255)
                    cacCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCa                                                                                                                                   >  1:1698639/1‑150 (MQ=255)
                     acCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCaa                                                                                                                                  >  2:477713/1‑150 (MQ=255)
                     acCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGGAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCaa                                                                                                                                  <  1:1571626/150‑1 (MQ=255)
                         tGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTAAACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGttt                                                                                                                              >  1:2053384/1‑150 (MQ=255)
                          gCTTAGTGGCTTTTCAGGCCCGGCAGTGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTg                                                                                                                             <  2:1852017/150‑1 (MQ=255)
                          gCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTg                                                                                                                             >  1:1636069/1‑150 (MQ=255)
                            ttAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGaa                                                                                                                           <  1:468955/150‑1 (MQ=255)
                             tAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGTGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAACTTTGaaa                                                                                                                          >  1:123874/1‑150 (MQ=255)
                             tAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGaaa                                                                                                                          <  1:920637/150‑1 (MQ=255)
                             tAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGCGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGaaa                                                                                                                          <  1:710774/150‑1 (MQ=255)
                               gTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGaaaaa                                                                                                                        >  2:995035/1‑149 (MQ=255)
                               gTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAag                                                                                                                        <  2:1164168/150‑1 (MQ=255)
                               gTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAag                                                                                                                        <  1:1874514/150‑1 (MQ=255)
                               gTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAag                                                                                                                        >  2:1709996/1‑150 (MQ=255)
                               gTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGAACAAATTTACAAAAATGCAAGTTAGaaaaa                                                                                                                        >  2:1979510/1‑149 (MQ=255)
                                   cTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTACATGAAGCACAAAATAACAAAAATGCAACTATGaaaaaacac                                                                                                                    >  2:1665880/1‑145 (MQ=255)
                                   cTTTTCAGGCCCCGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAACGGAAAAACTGTGGGGAAAGACTGTAAAGTAAGCACAAAATTACAAAAATGCAACTTTGAAAACAGAc                                                                                                                    >  2:1674805/1‑150 (MQ=255)
                                     tttCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACtt                                                                                                                  >  2:2113383/1‑150 (MQ=255)
                                        cAGGCCCGGCAGTGAAAAAGTGCGGAGTTAGTCAGATAATTAAAATCTACACGAGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTTGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAg                                                                                                               <  2:1712822/150‑1 (MQ=255)
                                          ggCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCg                                                                                                             <  1:1709996/150‑1 (MQ=255)
                                             ccGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTa                                                                                                          >  2:1313996/1‑150 (MQ=255)
                                             ccGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTa                                                                                                          >  1:1923036/1‑150 (MQ=255)
                                              cGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTAc                                                                                                         <  2:64644/150‑1 (MQ=255)
                                               ggCAGGTAAAAATTTAGGTGGTAGTGAGATAAGTAAAATCTACACGCGCGAATAAGTAGAACACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACa                                                                                                        <  2:1352659/150‑1 (MQ=255)
                                               ggCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCTAATAAGTAGACCACAGAAAAGGGAAAAAGTGTGGGGAAAGAGTGTGTATGAAGCACAAATTTACAAAAATGCAAGTTTGAAGAGAGACTTTAGCGTTACa                                                                                                        <  2:1598550/150‑1 (MQ=255)
                                               ggCAGGGAAAAAGTGCGGACGTAGTCAGATAAGTAAAATCTACACGCGCCAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACa                                                                                                        <  2:1150155/150‑1 (MQ=255)
                                                   gggAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAATCACAAATTTACAAAAATGCAATTTTTAAAAGATACTTTAGCGTTACaaaaa                                                                                                    >  2:1294257/1‑150 (MQ=255)
                                                   gggAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACaaaaa                                                                                                    >  1:1865243/1‑150 (MQ=255)
                                                    ggAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAATAGACTTTAGCGTTACAAAAAg                                                                                                   >  2:476948/1‑150 (MQ=255)
                                                    ggAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAg                                                                                                   >  2:798321/1‑150 (MQ=255)
                                                     gAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGc                                                                                                  <  1:521140/150‑1 (MQ=255)
                                                     gAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGc                                                                                                  >  1:1289088/1‑150 (MQ=255)
                                                     gAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTCCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGc                                                                                                  >  1:1633497/1‑150 (MQ=255)
                                                       aaaaGTGCGGAGGTAGTCAGATAATTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAg                                                                                                <  2:844336/150‑1 (MQ=255)
                                                         aaGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTa                                                                                              <  1:1132218/150‑1 (MQ=255)
                                                         aaGTGCGGAGGTAGTAATATAAGTAAAATCTACACGCGCGAATAAGTATACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTa                                                                                              <  2:976818/150‑1 (MQ=255)
                                                           gTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACTGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACATAAAGCAGTaaa                                                                                            >  1:273500/1‑150 (MQ=255)
                                                               ggAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAgg                                                                                        <  1:524579/150‑1 (MQ=255)
                                                                      gTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGtt                                                                                 <  2:792581/150‑1 (MQ=255)
                                                                        aaGATAATTAAAATCTACACGCCCGAATAAGTATACCACAGCAAACGGAAAAAGTGTGGGGAAAGAGTGTTCATCAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTAAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTaa                                                                               <  1:288010/149‑1 (MQ=255)
                                                                         aGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAAt                                                                              >  1:741093/1‑150 (MQ=255)
                                                                            tAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTgg                                                                           <  1:1929230/150‑1 (MQ=255)
                                                                              aGTAAAATCTACACGCGCGAATAATTAGACCACAGCAAAGGGAAAAAGTGTGGTGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGcc                                                                         <  1:1979510/150‑1 (MQ=255)
                                                                              aGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGcc                                                                         <  1:1495722/150‑1 (MQ=255)
                                                                               gTAAAATCTACACGCGCGAATATGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTAAAAAAAGCAGTAAAAAGGGCCAGTTAATTGGccc                                                                        <  2:1178428/150‑1 (MQ=255)
                                                                               gTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATAAAGCACAAATTTACAAAAATGCAAATTTGAAAAAAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAAGTGGccc                                                                        >  2:1334947/1‑150 (MQ=255)
                                                                                    aTCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAAtt                                                                   <  2:2041565/150‑1 (MQ=255)
                                                                                      cTACACGCGCGAATAAGTATACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCAAAAATTTACAAAAATGCAAGTTTGAAAAGAGAATTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAAtttt                                                                 <  2:1185907/150‑1 (MQ=255)
                                                                                        acacGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTAc                                                               <  1:1856211/150‑1 (MQ=255)
                                                                                               cgAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTGGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCAt                                                        >  2:498831/1‑150 (MQ=255)
                                                                                                gAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCAtt                                                       <  2:190639/150‑1 (MQ=255)
                                                                                                     aGTAGACCACAGCAAAGGGTAAAAGTGTGGTGAAAGAGTGTGCATGAAGCACAAATTTACATAAATGCAAGTTTGAAAAGAGACTTTTGCGTTACAAAAAGCAGTAAAAAGCGCCAGTTAATTGGCCCAAATTTTACGAGTCATTGTTta                                                  <  2:1329056/150‑1 (MQ=255)
                                                                                                       tAGACCACAGCAAAGTGAAAAAGTGTGTGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCATTGTTtata                                                <  1:327108/150‑1 (MQ=255)
                                                                                                       tAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCATTGTTtata                                                <  2:919826/150‑1 (MQ=255)
                                                                                                       tAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCATTGTTtata                                                <  2:1585538/150‑1 (MQ=255)
                                                                                                       tAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCATTGTTtata                                                >  2:936503/1‑150 (MQ=255)
                                                                                                           ccACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTTAATAAATCAAAAAATAACAAAAATCCAAATAATAAAAAAAAATTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCGCAAATTTTACGAGTCATTGTTTATACAtt                                            >  2:1505385/1‑150 (MQ=255)
                                                                                                             acaGCAAAGTGAAAAAGTGTGGGGAAAGAGTGTGCATGAATCACAAATTTAAAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCATTGTTTATACATTTc                                          <  2:1255726/150‑1 (MQ=255)
                                                                                                              caGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCATTGTTTATACATTTCg                                         <  1:42893/150‑1 (MQ=255)
                                                                                                              caGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGAATGAAGCACAAAATTACAAAAATGCAAGTTTTAAAAGAAACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCATTGTTTATACATTTCg                                         >  2:1416627/1‑150 (MQ=255)
                                                                                                              caGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTAAATAAATCAAAAAATAACAAAAATCCAAATAATAAAAAAAAAAATAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGACCAAATTTTACGAGTCATTGTTTATACATTTCg                                         >  2:272799/1‑150 (MQ=255)
                                                                                                                gCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCATTGTTTATACATTTCGtt                                       <  2:11107/150‑1 (MQ=255)
                                                                                                                  aaaGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTTAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCATTGTTTATAAATTTCGTTGa                                     >  2:2037697/1‑150 (MQ=255)
                                                                                                                   aaGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCATTGTTTATACATTTCGTTGAt                                    >  2:1766538/1‑150 (MQ=255)
                                                                                                                    aGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCATTGTTTATACATTTCGTTGATg                                   <  2:2120750/150‑1 (MQ=255)
                                                                                                                     gggAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCATTGTTTATACATTTCGTTGATGc                                  <  2:2053384/150‑1 (MQ=255)
                                                                                                                      ggAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTCTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCATTGTTTATACATTTCGTTGATGCg                                 <  1:820460/150‑1 (MQ=255)
                                                                                                                       gAAAAAGTGTGGGGAAAGAGTGTGCATGAATCACAAATTTACAAAAATCCAACTATGAAAAGATACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCATTGTTTATACAGTTCGTTGATGCGt                                >  2:622741/1‑150 (MQ=255)
                                                                                                                       gAAAAAGTGTGGGGAAAGAGTGTGAATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCATTGTGTATACATTTCGTTGATGCGt                                >  2:631100/1‑150 (MQ=255)
                                                                                                                          aaaGTGTGGGGAAAGAGTGTTAATGAAGCACAAATTAACAAAAATGCAAGTTTGAAAAGAGAATTTCGCTATACAAAAAGCAGTAAAACGGCCCAGTTATTGGGCCAAAATTTAACGAGTAATTTTTTATACATTTAGTTGATGAGTTTg                             >  2:2160190/1‑150 (MQ=255)
                                                                                                                             ttgtgGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTATCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCATTGGTTATACATTTCGTTGATGCGTTTGATg                          <  1:261120/149‑1 (MQ=255)
                                                                                                                             gtgtTGGGAAAGAGTGTAAATAAATCAAAAAATAACAAAAATCCAAATAATAAAAAAAAAAATAGCGTTACAAAACGCAGTAAAAAGGGCCAGGTAATTGGCCCAAATTTTACGAGTCATTGTTAATACATTTCGTTGATGCGTTTGCTg                          >  2:219606/1‑150 (MQ=255)
                                                                                                                             gtgtAGGGAAAGAGTGTAAATAAATCAAAAAATAACAAAAATCCAAATAATAAAAAAAAAAATAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTACTTGGCCCAAATTTTACGAGTCATTGTTTATACATTTCGTGGATGCGTTTGATg                          >  2:226796/1‑150 (MQ=255)
                                                                                                                              tgtgGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTAAAAAAATCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCATTGTTTATACATTTCGTTGATGCGTTTGATGc                         <  2:1636069/150‑1 (MQ=255)
                                                                                                                                tgGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCATTGTTTATACATTTCGTTGATGCGTTTGATGcac                       >  2:1542165/1‑150 (MQ=255)
                                                                                                                                 ggggAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTTAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCATTTAATTGGCCCAAATTTTACGAGTCATTGTTTATACATTTCGTTGATGCTTATGATGcaca                      >  2:850265/1‑150 (MQ=255)
                                                                                                                                    gAAAGAGTGTGCATGAAGCACAAATTTACAATCAAGCAAGTTTGAAAAGAGACTTTAGCGCTACAAAAAGCAGTAAAAAGGGCGAGATAAATTGCCCAAATTTTACGAGTCATTGTTTAAACATTTCGTTGATGCGTTTGATGCACACgg                   <  2:371602/150‑1 (MQ=255)
                                                                                                                                     aaaGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCATTGTTTATACATTTCGTTGATGCGTTTGATGCACACGGa                  <  1:997485/150‑1 (MQ=255)
                                                                                                                                        gagTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCATTGTTTATACATTTCGTTGATGCGTTTGATGCACACGGAAGc               <  1:599499/150‑1 (MQ=255)
                                                                                                                                          gtgtGCATGAAGCACTAATATACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCATTGTTTATACATTTCGTTGATGCGTTTGATGCACACGGAAGCTg             <  2:1822059/150‑1 (MQ=255)
                                                                                                                                          gtgtGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGGTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCATTGTTTATACATTTCGTTGATGCGTTTGATGCACACGGAAGCTg             <  2:1923036/150‑1 (MQ=255)
                                                                                                                                           tgtgCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCATTGTTTATACATTTCGTTGATGCGTTTGATGCACACGGAAGCTGa            >  1:2068572/1‑150 (MQ=255)
                                                                                                                                            gtgCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCATTGTTTATACATTTCGTTGATGCGTTTGATGCACACGGAAGCTGaa           <  2:408743/150‑1 (MQ=255)
                                                                                                                                               cATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGCGACTTTAGCGTTCCAAAAAGCAGTAAAAAGGGCCAGGTAATTGGCCCAAATTTTACGAGTCATTGATTATACATTTCGTTGATGCGTTTGATGAACCCGGAAGCTGAAGcc        >  1:1080141/1‑150 (MQ=255)
                                                                                                                                                aTGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCATTGTTTATACATTTCGTTGATGCGTTTGATGCACACGGAAGCTGAAGccc       <  1:477713/150‑1 (MQ=255)
                                                                                                                                                aTGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCATTGTTTATACATTTCGTTGATGCGTTTGATGCACACGGAAGCTGAAGccc       >  1:2094289/1‑150 (MQ=255)
                                                                                                                                                   aaGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCATTGTTTATACATTTCGTTGATGCGTTTGATGCACACGGAAGCTGACGCCCAGt    >  2:892554/1‑150 (MQ=255)
                                                                                                                                                    aGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCATTGTTTATACATTTCGTTGATGCGTTTGATGCACACGGAAGCTGAAGCCCAGTa   >  2:250719/1‑150 (MQ=255)
                                                                                                                                                    aGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCATTGTTTATACATTTCGTTGATGCGTTTGATGCACACGGAAGCTGAAGCCCAGTa   <  1:995035/150‑1 (MQ=255)
                                                                                                                                                     gCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCATTGTTTATACATTTCGTTGATGCGTTTGATGCACACGGAAGCTGAAGCCCAGTAg  >  1:835402/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CCTGGACAAACAGGTGATAACACCCTGCTTAGTGGCTTTTCAGGCCCGGCAGGGAAAAAGTGCGGAGGTAGTCAGATAAGTAAAATCTACACGCGCGAATAAGTAGACCACAGCAAAGGGAAAAAGTGTGGGGAAAGAGTGTGCATGAAGCACAAATTTACAAAAATGCAAGTTTGAAAAGAGACTTTAGCGTTACAAAAAGCAGTAAAAAGGGCCAGTTAATTGGCCCAAATTTTACGAGTCATTGTTTATACATTTCGTTGATGCGTTTGATGCACACGGAAGCTGAAGCCCAGTAG  >  NC_000913/3710486‑3710784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑