Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,781,131 T→G 7.7% intergenic (+114/‑84) lldD → / → trmL L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,781,1310TG 7.7% 228.6 / 5.2 95intergenic (+114/‑84)lldD/trmLL‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base T (41/44);  new base G (5/3);  total (47/48)
Fisher's exact test for biased strand distribution p-value = 4.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGC  >  NC_000913/3780984‑3781280
                                                                                                                                                    |                                                                                                                                                     
ccTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTcc                                                                                                                                                     <  1:856932/149‑1 (MQ=255)
   ggCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCggg                                                                                                                                                  <  1:260263/150‑1 (MQ=255)
    gCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCgggg                                                                                                                                                 <  1:307710/150‑1 (MQ=255)
    gCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCgggg                                                                                                                                                 <  2:823281/150‑1 (MQ=255)
      tCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGgag                                                                                                                                               <  1:1459781/150‑1 (MQ=255)
       cccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGgaga                                                                                                                                              <  2:1274314/150‑1 (MQ=255)
       cccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGgaga                                                                                                                                              <  2:505338/150‑1 (MQ=255)
       cccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCAGAAACGCCACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGgaga                                                                                                                                              >  2:1321255/1‑150 (MQ=255)
        ccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACACCCAAACCCACACCCTCAGGGAGCGGGGCCCGGTAGGGGCGGTATGTAATCCCGCACTCTGAAACGCCCCCGCCCGCTTTTTTTATCCCGCGCCCCGCCggggcgcg                                                                                                                                             >  1:198039/1‑146 (MQ=255)
          aTGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCCGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAggg                                                                                                                                           <  2:776108/150‑1 (MQ=255)
          aTGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAggg                                                                                                                                           <  1:518399/150‑1 (MQ=255)
          aTGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAggg                                                                                                                                           <  2:784069/150‑1 (MQ=255)
               taaaGGGAAGGCGGCATAGGCGTTTGCGCCCCCTCCCCCTACCCCTCTCCCTCTGGTAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAACCGACCCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCgggg                                                                                                                                     <  2:1023829/149‑1 (MQ=255)
               gAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCATGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCggg                                                                                                                                      <  2:1623463/150‑1 (MQ=255)
                aaaGGGAATGCCGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCgggg                                                                                                                                     <  2:439532/150‑1 (MQ=255)
                 aaGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGt                                                                                                                                    <  2:2155308/150‑1 (MQ=255)
                  aGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTg                                                                                                                                   <  2:1035387/150‑1 (MQ=255)
                    ggAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTTTGTACTCCCTCACTGTGTAACGCCCCCGCACGCGTTTTTTCTCCCGCGCCCCTCCGGGGAGAGGGCCGGGGTGAg                                                                                                                                 >  1:1438540/1‑150 (MQ=255)
                     gAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAgg                                                                                                                                <  1:71409/150‑1 (MQ=255)
                     gAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAgg                                                                                                                                <  2:219651/150‑1 (MQ=255)
                     gAATGCGGCATAGTAGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGTAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAgg                                                                                                                                <  2:1390903/150‑1 (MQ=255)
                      aaTGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACGCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAggg                                                                                                                               >  1:1175319/1‑150 (MQ=255)
                       aTGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAgggg                                                                                                                              <  1:689744/150‑1 (MQ=255)
                         gCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGaa                                                                                                                            <  2:2040379/150‑1 (MQ=255)
                            gCATAGTGGTTTGCGCCCCCTCACCCTATCCCTCTCCCTCAGTGAGAGGGGACCGTTCGGCGCTGTTTGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAg                                                                                                                         <  2:1967255/150‑1 (MQ=255)
                            gCATAGTCGTTTTCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAg                                                                                                                         <  2:1562417/150‑1 (MQ=255)
                              ttAGGCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGc                                                                                                                       <  1:1548616/149‑1 (MQ=255)
                              aTAGTGGTTTGCGCCCCCTCACCCTACCCCTCTCCCACAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGc                                                                                                                       <  1:1433725/150‑1 (MQ=255)
                              aTAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCCGGGAGAGGGGACGGTTCGGCGCTGTAGGTACTCCCTCACTCTGAAACCACACCCCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGc                                                                                                                       <  1:1321805/150‑1 (MQ=255)
                                 gTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGTGAGGGGACCGTTCGGCGTTGTATGTACTCCCTCACTCTGAAACGACACCGCACGCTTTTTTTCTCCCTCGCCCCTCCGGGTAGTGGGCGGGGGTGTGGGGAAAAGGCCGc                                                                                                                    <  2:256498/150‑1 (MQ=255)
                                       tGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCGGCAACGCCACCGCCCTCTTTTTTTCTCCCTCGCCCCGCCGGGGGGAGGCCGGGGGGGCGGGGAAAGGGCCGCAGTggg                                                                                                              >  1:54920/1‑148 (MQ=255)
                                            ccccTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCa                                                                                                         >  1:505625/1‑150 (MQ=255)
                                             cccTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCaa                                                                                                        >  1:52427/1‑150 (MQ=255)
                                             cccTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACGCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGGGAGGGGAAAAGGCCGCACTGCTcccgaa                                                                                                        >  2:2159075/1‑145 (MQ=255)
                                                  ccccacaCCCGCCCCCTCCGGGCGTGGGGACCGGGCGGCGCTGTATGTCCGCCCCCACTCTGAAACGACCCCGCACTCTTTTTTTCTCCCGCGCCCCTCCGGGGCGAGGGCCGGGGTGCGGGGAAAAGGCCGCACTGCTCACCAATTTTc                                                                                                   <  1:1157966/144‑1 (MQ=255)
                                                   cccTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCt                                                                                                  >  2:870141/1‑150 (MQ=255)
                                                   cccTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCt                                                                                                  >  1:1215289/1‑150 (MQ=255)
                                                   cccTAACCCTCACCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCACACTCTGAACCGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCt                                                                                                  <  1:1572227/150‑1 (MQ=255)
                                                         cccTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATc                                                                                            >  2:2085617/1‑150 (MQ=255)
                                                          ccgctcCCTCAGGGAGAGGGGACCGTGGGGCGCGGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCGTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATcc                                                                                           <  2:1416938/147‑1 (MQ=255)
                                                           ctctCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAACCGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGATGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCt                                                                                          >  1:2009522/1‑150 (MQ=255)
                                                               cccTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGccc                                                                                      >  1:1863822/1‑150 (MQ=255)
                                                               cccTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGccc                                                                                      >  1:132027/1‑150 (MQ=255)
                                                                ccTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGcccc                                                                                     >  2:841460/1‑150 (MQ=255)
                                                                              gACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCGTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGGGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGc                                                                       >  2:142618/1‑150 (MQ=255)
                                                                                ccGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAACGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAg                                                                     >  1:1912351/1‑150 (MQ=255)
                                                                                               tgtcCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGt                                                      <  2:1438540/150‑1 (MQ=255)
                                                                                                gtaCTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCATCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTa                                                     >  1:1306279/1‑150 (MQ=255)
                                                                                                 taCTCCCTCACTCTGAAACGACACCGCAGTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTAc                                                    >  2:1159570/1‑150 (MQ=255)
                                                                                                    cccccCACTCTTAAACCACACCGCATTATTTTTTCCCCCCCCGCCCCTCCGGGGTGTGGGCCGGGGTGCGGGCACAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACttt                                                 <  2:1802676/149‑1 (MQ=255)
                                                                                                      ccTCACTCTGAACCGACACCGCACTCTTTTTTTCACCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTAc                                               <  2:1519816/150‑1 (MQ=255)
                                                                                                      acTCAATCTTATAGGACACCTCTCTTTTTTTTTCTCCCTCGCCCGTCCGGGGTGTGGGCGGGGGGGTGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTAc                                               <  2:778484/149‑1 (MQ=255)
                                                                                                        tCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCCCCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGa                                             >  1:489979/1‑150 (MQ=255)
                                                                                                        tCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGa                                             >  2:249108/1‑150 (MQ=255)
                                                                                                         ccctctGAACCGACCCCGCACTCTTTTTTTCTCCCGCGCCCCTCCGGGGAGTGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGaa                                            <  1:1576727/148‑1 (MQ=255)
                                                                                                           ctctGAACCGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAAcc                                          <  1:1292562/150‑1 (MQ=255)
                                                                                                           ctctGAAACGACACCGCAGTCTTTTTTTCTCCAGCGCCCCTCGGGGTTGTGGGCGGGGGTGAGTGGAAAAGGCCGCACTGCGCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTATACATCGTACTTTACGAAcc                                          <  2:35566/150‑1 (MQ=255)
                                                                                                           ctctGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAAcc                                          >  2:1691205/1‑150 (MQ=255)
                                                                                                             ctGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAg                                        >  2:191892/1‑150 (MQ=255)
                                                                                                              tGAAACGACACCGCACTCTTATTTTCTCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGCCAGTATGCTAAACATCGAAATTTAAGAACCAGaa                                      >  2:351439/1‑150 (MQ=255)
                                                                                                                aaaCGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACCTCGTACTTTACGAACCAGaaa                                     >  1:1960419/1‑150 (MQ=255)
                                                                                                                    cacacCCCCCTCTTTTTTCCGCCCGCCCCCCCCCGGGCAGAGGGCCGGGGTGAGGGTAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTcc                                 <  1:585586/149‑1 (MQ=255)
                                                                                                                     cccccGCACTGTTTTTTACCCCCCCGCCCCGCGGGGGTGGGGGCGGGGGTGTGGTGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCg                                <  1:681452/147‑1 (MQ=255)
                                                                                                                       acCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTCCGAACCAGAACTTCCGcc                              >  2:397107/1‑150 (MQ=255)
                                                                                                                        ccGCCCTCTTTTTGTCACCCCCGCCCCGCCGGGCCGCGGGCCGGGGGGAGGGGACACGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCa                             <  2:1061695/150‑1 (MQ=255)
                                                                                                                        ccGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCCGCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTTCTGTACGAACCAGAACTTCCGCCa                             >  2:542789/1‑150 (MQ=255)
                                                                                                                             ctctTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAACTAc                        >  1:794534/1‑150 (MQ=255)
                                                                                                                              tctTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACt                       >  2:962394/1‑150 (MQ=255)
                                                                                                                               ctTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGCACCAGAAATTCCGCCAATTACTg                      >  1:920519/1‑150 (MQ=255)
                                                                                                                                tttttttCTCCCTCGCCCCTCGGGGGAGTGGGCCGGGGGGAGGGTAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTgg                     <  2:198039/150‑1 (MQ=255)
                                                                                                                                tttttttCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTgg                     >  2:2060731/1‑150 (MQ=255)
                                                                                                                                  tttttCGCCCTCGCGGCTCCGGTGAGAGGTGCGGGGGGCGTGGAAAAGGCCGCACTGCTGACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCa                   <  1:97681/150‑1 (MQ=255)
                                                                                                                                  ttttcCCCCGTCGCCCCTCGGGGTTGCGGGCGGGGGTGTGGGTAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCa                   <  1:997483/150‑1 (MQ=255)
                                                                                                                                   ttttCCCCCCCCCCCCGCGGGGGTGGGGGCGGGGGTGTGGGTAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCaa                  <  1:1877877/150‑1 (MQ=255)
                                                                                                                                    tttCTCCCTCGCCCCTCGGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAAc                 <  2:1322971/150‑1 (MQ=255)
                                                                                                                                    cccggcccTCTCCCCGGAGGGGCGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCCCTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAAc                 >  1:1811053/6‑150 (MQ=255)
                                                                                                                                     ttCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAACCATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAAca                >  1:1512133/1‑150 (MQ=255)
                                                                                                                                     ccggcccTCTCCCCGGAGGGGCGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCCCCATTTTTCTGCTATCCTGCCCCGGCCCGAAGGGGGCAGTATGCTAAACAGCGTACTTTACGATCCAGAAAGTCCGCCTAATACTGTCatcg                >  1:382227/5‑147 (MQ=255)
                                                                                                                                        tcCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGCACCAGAAATTCCGCCAAATACTGGCAAcatca             >  1:1072278/1‑150 (MQ=255)
                                                                                                                                        tcCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAAcatca             <  1:1321255/150‑1 (MQ=255)
                                                                                                                                        tcCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAAcatca             >  1:1534067/1‑150 (MQ=255)
                                                                                                                                        gcccTCTCCCCGGAGGGGCGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGGACTTTACGCCCCAGAAATTCCGCCAAATACGGGCAAcatcc             >  1:550679/2‑149 (MQ=255)
                                                                                                                                         cccTCGCCCCCCCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAAcatca             >  2:1974310/1‑150 (MQ=255)
                                                                                                                                          ccTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCaa                  >  2:1455402/1‑143 (MQ=255)
                                                                                                                                          ccTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCaa                  <  1:1455402/143‑1 (MQ=255)
                                                                                                                                          ccTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACatcatc           <  2:139246/150‑1 (MQ=255)
                                                                                                                                            tCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACAGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGCACCAGAAATTCCGCCAAATACTGGCAACATCATCCg         >  1:701683/1‑150 (MQ=255)
                                                                                                                                             cGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTGTCCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGGATGCTGAGCAGCGTACTTGACGAACCAGACATGCCGGCAAATACTGGCCTCAGCTTCCGt        >  1:332166/1‑150 (MQ=255)
                                                                                                                                              gCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTc       >  1:2185979/1‑150 (MQ=255)
                                                                                                                                                ccccTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCCCTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGAGCAGTATGCTTAACATCGTACTTTACGCACCAGAATTTCCGCCAAAAACTGGGCGcatcatgaggct      >  2:1626153/1‑144 (MQ=255)
                                                                                                                                                ccccTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCt      <  2:1295057/150‑1 (MQ=255)
                                                                                                                                                ccccTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCt      >  2:1536847/1‑150 (MQ=255)
                                                                                                                                                 cccTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGACCCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCtt     >  1:2201543/1‑150 (MQ=255)
                                                                                                                                                  ccTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCttt    >  1:714429/1‑150 (MQ=255)
                                                                                                                                                   cTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCGCTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTg   >  2:1603784/1‑150 (MQ=255)
                                                                                                                                                    tCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTgc  >  1:1003636/1‑150 (MQ=255)
                                                                                                                                                    tCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTgc  >  1:272030/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
ACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGC  >  NC_000913/3780984‑3781280

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: