Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 265,582 T→A 5.8% intergenic (‑39/+38) ykfF ← / ← ykfB CP4‑6 prophage; uncharacterized protein/CP4‑6 prophage; uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913265,5820TA 5.8% 377.5 / 8.4 130intergenic (‑39/+38)ykfF/ykfBCP4‑6 prophage; uncharacterized protein/CP4‑6 prophage; uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base T (62/60);  new base A (4/4);  total (66/64)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.34e-01

Only 100 of 132 total aligned reads displayed.

GAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGTCGAACTGCCCGGC  >  NC_000913/265433‑265730
                                                                                                                                                     |                                                                                                                                                    
gAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCt                                                                                                                                                      >  2:679658/1‑150 (MQ=255)
 aaGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTa                                                                                                                                                     <  2:695749/150‑1 (MQ=255)
 aaGTGACTGCCATGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTTACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTa                                                                                                                                                     <  2:1583688/150‑1 (MQ=255)
  aGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAg                                                                                                                                                    <  1:86526/150‑1 (MQ=255)
   gtcaCTGCCCTGGGCGTCTTCGAGGGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTGGTCTCCGGGTGAAATGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGc                                                                                                                                                   <  1:154856/147‑1 (MQ=255)
      aCTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTATCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGt                                                                                                                                                >  1:1885992/1‑150 (MQ=255)
          cccTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAACTGTCggg                                                                                                                                            >  1:1493454/1‑150 (MQ=255)
          cccTGGTCGTCTTCGAGTGGGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGACAGCTAGCAGGCTGCCggg                                                                                                                                            <  2:1284599/150‑1 (MQ=255)
          cccTGGTCGTCATCGAGTGTGATATTGCTGTACGTGGTAGTGAAAGCTTGCGCTTGTCTCCGGGTGAAAGGACCAGGTGGCAGCAACACGCATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCAGCTAGCTGTCggg                                                                                                                                            >  2:758634/1‑150 (MQ=255)
          cccTGGTCGGCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCggg                                                                                                                                            <  2:1163510/150‑1 (MQ=255)
          cccTGGGAGTCTTCGAGTGTTATATTGCTGTAAGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGCAAGGCCCCGGTGTAAGCAACACGGATTGGGTCATTTTCGGGAGCCTGATAAAAGAAAACCCCGACAGCTAGCATGCTGCCggg                                                                                                                                            <  2:1426520/150‑1 (MQ=255)
           ccTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGTAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGACAGCTAGCAGGCTGCCgggg                                                                                                                                           <  2:1007505/150‑1 (MQ=255)
           ccTGGTCGTCTTCGAGTGTGATATTGCTGTACGGGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCgggg                                                                                                                                           <  1:1019878/150‑1 (MQ=255)
             tGGTCGTCTTCGTGTGTGATATTGCTGTACGGGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCTTTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTg                                                                                                                                         <  1:2024893/150‑1 (MQ=255)
               gtcgtcTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGaa                                                                                                                                       >  1:358554/1‑150 (MQ=255)
                    cTTCGAGTGTGATATTGCGGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGc                                                                                                                                  <  1:1434800/150‑1 (MQ=255)
                      tCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTg                                                                                                                                >  2:841242/1‑150 (MQ=255)
                       cGAGTGTGATATTGCTGTACGTTGTAGTGACCGCTTGCGCTTGTCTCAGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTCTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTgg                                                                                                                               <  2:406434/150‑1 (MQ=255)
                             tgATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGa                                                                                                                         >  1:74939/1‑150 (MQ=255)
                              gATAGTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGGGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGAt                                                                                                                        <  2:1791680/150‑1 (MQ=255)
                                 atTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGAtact                                                                                                                     <  1:1706803/150‑1 (MQ=255)
                                 atTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGAtact                                                                                                                     >  1:867630/1‑150 (MQ=255)
                                 atTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGAtact                                                                                                                     >  2:781638/1‑150 (MQ=255)
                                 atTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGAtact                                                                                                                     <  2:639973/150‑1 (MQ=255)
                                  ttGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGAtacta                                                                                                                    >  1:504860/1‑150 (MQ=255)
                                   tGCTGTCCGTGGTAGTGACCGCTTGCGATTGTCTACGGGTGAAAGGCCCCTGTGGCAGCAACACGGATTGGGTCATTTTCGGGATCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGGCGGGGTGAATTTGCTGGGGAAGAtactac                                                                                                                   <  2:384902/150‑1 (MQ=255)
                                   tGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGAtactac                                                                                                                   <  2:1532509/150‑1 (MQ=255)
                                    gCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTATCGGGCTCCTGATAAAAGCAAACCCCGGCAGCCTGCTAGCTGGCGGGGGGAATTTGCTGGGGAAGATactact                                                                                                                  >  2:122806/1‑150 (MQ=255)
                                           gTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTc                                                                                                           <  2:504860/150‑1 (MQ=255)
                                            tGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCg                                                                                                          <  2:820508/150‑1 (MQ=255)
                                              gTAGTGACCGCTTGCGCTTGTCTCCGGGTGAACGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGtt                                                                                                        >  2:1618313/1‑150 (MQ=255)
                                               tAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGGGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTTATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTtg                                                                                                       <  2:107183/150‑1 (MQ=255)
                                                  tGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTtgctg                                                                                                    <  1:2020037/150‑1 (MQ=255)
                                                   gACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTtgctgc                                                                                                   <  1:2201315/150‑1 (MQ=255)
                                                   gACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTtgctgc                                                                                                   >  2:1522897/1‑150 (MQ=255)
                                                   gACCGCGTGCGCTTGTCGCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCGCCTGATAAAAGAAAACCCCGGCAGGCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGGtgcggc                                                                                                   <  1:2037448/150‑1 (MQ=255)
                                                     ccGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAg                                                                                                 <  2:250932/150‑1 (MQ=255)
                                                     ccGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAg                                                                                                 <  1:1032979/150‑1 (MQ=255)
                                                       gcttgcgcttgTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGtc                                                                                               >  2:900894/1‑150 (MQ=255)
                                                       gcttgcgcttgTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGCCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGtc                                                                                               <  1:1443923/150‑1 (MQ=255)
                                                            cgcttgTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCga                                                                                          >  1:1596463/1‑150 (MQ=255)
                                                            cgcttgTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTAGTTGCTGCAGTCTCCga                                                                                          >  2:1047918/1‑150 (MQ=255)
                                                             gcttgTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCgag                                                                                         <  1:773776/150‑1 (MQ=255)
                                                                tttctcCGGGTGAAAGGCCCCGGGGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGt                                                                                      <  1:293515/148‑1 (MQ=255)
                                                                  tctcCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTgg                                                                                    >  1:1318944/1‑150 (MQ=255)
                                                                   ctcCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAAATTCCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGa                                                                                   >  2:226616/1‑150 (MQ=255)
                                                                   ctcCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGGCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGa                                                                                   <  1:1195388/150‑1 (MQ=255)
                                                                   ctcCGGGTGAAACGCCCCGGTGGCAACAACACGGATTGGGTAATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCAGCAAGCTGACGGGCTGAATTTGCTCGGGAAGATACTACTATCAGTCCTTGCTGCAGTCTCCGAGAGTGGa                                                                                   >  1:1655300/1‑150 (MQ=255)
                                                                    tcCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCACGGCAGCCTGCTAGCTGTCGGGGAGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGCAc                                                                                  >  2:1759386/1‑150 (MQ=255)
                                                                           gAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAATGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACtt                                                                           >  2:118515/1‑150 (MQ=255)
                                                                           gAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACtt                                                                           >  2:1585929/1‑150 (MQ=255)
                                                                            aaaGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACttt                                                                          >  2:224205/1‑150 (MQ=255)
                                                                             aaGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTtc                                                                         >  2:889116/1‑150 (MQ=255)
                                                                             aaGGCCCCGGTGGCAGCAACACGGATTGGGTCATTATCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGGCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTtc                                                                         <  1:1165183/150‑1 (MQ=255)
                                                                              aGGCCCCGGTGGCAGCAACACGGTTTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCTGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTtct                                                                        <  1:550991/150‑1 (MQ=255)
                                                                              aGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTtct                                                                        >  1:1040665/1‑150 (MQ=255)
                                                                                 ccccGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAg                                                                     <  2:568328/150‑1 (MQ=255)
                                                                                     ggTGGCAGCGACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGtt                                                                 >  2:900392/1‑150 (MQ=255)
                                                                                      gTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGttc                                                                >  1:1902814/1‑150 (MQ=255)
                                                                                      gTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGttc                                                                <  2:544442/150‑1 (MQ=255)
                                                                                       tGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGttct                                                               >  2:1131478/1‑150 (MQ=255)
                                                                                          cagcaACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTcc                                                            <  2:2184072/150‑1 (MQ=255)
                                                                                          cagcaACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTcc                                                            >  2:1418763/1‑150 (MQ=255)
                                                                                          cagcaACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTcc                                                            <  1:781638/150‑1 (MQ=255)
                                                                                                  cGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCa                                                    >  2:1876984/1‑150 (MQ=255)
                                                                                                   ggATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAg                                                   <  1:1138535/150‑1 (MQ=255)
                                                                                                      ttGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGa                                                <  1:1322073/150‑1 (MQ=255)
                                                                                                        gggTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAg                                              >  2:1134873/1‑150 (MQ=255)
                                                                                                          gTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGt                                            >  1:1633872/1‑150 (MQ=255)
                                                                                                           tCATTTTCGGGCGCCTGATACAAGAACTCCCCGGGAGCCGGCTAGCGGTCGGGGTTAATTTGCTGGGGAAGATACTACTCTCAGTCGTTGCGGCAGTCTCCGAGAGTGGCCAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTc                                           <  1:2039691/150‑1 (MQ=255)
                                                                                                            cATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTcc                                          >  1:1378049/1‑150 (MQ=255)
                                                                                                                    ggCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAACTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGGCCGCAGTGAAGGTCCCTGCTTtg                                  <  2:1493454/150‑1 (MQ=255)
                                                                                                                      cTCCTGATAAAAGAAACCCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTtgtg                                <  2:1093908/150‑1 (MQ=255)
                                                                                                                      cTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTtgtg                                <  2:2161374/150‑1 (MQ=255)
                                                                                                                      cTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTtgtg                                >  2:76955/1‑150 (MQ=255)
                                                                                                                       tCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTgg                               <  2:74939/150‑1 (MQ=255)
                                                                                                                       tCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTgg                               >  2:461966/1‑150 (MQ=255)
                                                                                                                         cTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTc                             >  1:218693/1‑150 (MQ=255)
                                                                                                                          tGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCGGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCa                            >  2:2168855/1‑150 (MQ=255)
                                                                                                                          tGATAAAAGAAAACCCCGGCAGCCTCCTAGATGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCa                            >  2:475364/1‑150 (MQ=255)
                                                                                                                           gATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCAt                           >  1:2140554/1‑150 (MQ=255)
                                                                                                                             tAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTg                         >  1:785315/1‑150 (MQ=255)
                                                                                                                               aaaGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGAc                       >  2:1052388/1‑150 (MQ=255)
                                                                                                                               aaaGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGAc                       <  1:483042/150‑1 (MQ=255)
                                                                                                                               aaaGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGAc                       >  2:582280/1‑150 (MQ=255)
                                                                                                                                aaGAAAACCCCGGCTGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTGTATCAGCGTTCTTCCGGTCCGCAGTGATGGTCGCTGCTTTGTGGTCATTGACg                      >  1:1058601/1‑150 (MQ=255)
                                                                                                                                 aattcaCCCCGACAGCTAGCAGGCTGCCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGt                     >  1:1266938/6‑150 (MQ=255)
                                                                                                                                  gAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTa                    >  1:1185893/1‑150 (MQ=255)
                                                                                                                                  gAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTa                    <  2:2076535/150‑1 (MQ=255)
                                                                                                                                   aaacCCCCGGCCGCCTGCTTGCTGTGGGGGTGAATTTGCTGGTGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAg                   <  2:1089695/150‑1 (MQ=255)
                                                                                                                                    aaaCCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAgc                  >  1:1863228/1‑149 (MQ=255)
                                                                                                                                     aaCCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGAc                 <  1:977911/150‑1 (MQ=255)
                                                                                                                                      aCCCCGACAGCTAGCAGGCTGCCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGATGGTCCCTGCTTTGTGGTCATTGACGTAGACg                >  1:1107450/1‑150 (MQ=255)
                                                                                                                                      aCCCCGACAGCTAGCAGGCTGCCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACg                >  1:810770/1‑150 (MQ=255)
                                                                                                                                       ccccGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGt               >  1:907117/1‑150 (MQ=255)
                                                                                                                                         ccGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGTCg             >  1:523729/1‑150 (MQ=255)
                                                                                                                                          cGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAATATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGTCGa            <  1:841242/150‑1 (MQ=255)
                                                                                                                                               gCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAACGTCCCTGCTTTGTGGTCATTGACGTAGACGTCGAACTGc       >  1:2123684/1‑150 (MQ=255)
                                                                                                                                                ccTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGTCGAACTGcc      >  1:1125735/1‑150 (MQ=255)
                                                                                                                                                  tGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGTCGAACTGCCCg    >  2:468807/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GAAGTGACTGCCCTGGTCGTCTTCGAGTGTGATATTGCTGTACGTGGTAGTGACCGCTTGCGCTTGTCTCCGGGTGAAAGGCCCCGGTGGCAGCAACACGGATTGGGTCATTTTCGGGCTCCTGATAAAAGAAAACCCCGGCAGCCTGCTAGCTGTCGGGGTGAATTTGCTGGGGAAGATACTACTATCAGTCGTTGCTGCAGTCTCCGAGAGTGGACAGAACTTTCTCAGCGTTCTTCCGGTCCGCAGTGAAGGTCCCTGCTTTGTGGTCATTGACGTAGACGTCGAACTGCCCGGC  >  NC_000913/265433‑265730

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: