Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,923,717 G→A 8.6% intergenic (‑37/+27) rsmG ← / ← mnmG 16S rRNA m(7)G527 methyltransferase, SAM‑dependent; glucose‑inhibited cell‑division protein/5‑methylaminomethyl‑2‑thiouridine modification at tRNA U34

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,923,7170GA 8.6% 482.0 / 21.7 161intergenic (‑37/+27)rsmG/mnmG16S rRNA m(7)G527 methyltransferase, SAM‑dependent; glucose‑inhibited cell‑division protein/5‑methylaminomethyl‑2‑thiouridine modification at tRNA U34
Reads supporting (aligned to +/- strand):  ref base G (82/65);  new base A (11/3);  total (93/68)
Fisher's exact test for biased strand distribution p-value = 1.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.23e-01

Only 100 of 164 total aligned reads displayed.

TACGCTTTGTTCCATTTATGCAGCATATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATCGTTA  >  NC_000913/3923568‑3923864
                                                                                                                                                     |                                                                                                                                                   
tACGATTTGTTCCTTTTATGCTGCTTTTTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTATGCGAAATACCTGCGTCTTTCAGCAGTAAGTAGAGTTTGTTGATCACTGTGATTTCCTGTTCTTGATGCGTTGCCTGGTAAGCg                                                                                                                                                     <  1:1396414/150‑1 (MQ=255)
 aCGCTTTGTTCCATTTATGCAGCATATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCgg                                                                                                                                                    <  2:14003/150‑1 (MQ=255)
 aCGCTTTGTTCCATTTATGCAGCATATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCgg                                                                                                                                                    >  2:22693/1‑150 (MQ=255)
    cTTTGTTCCATTTATGCAGCATATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTg                                                                                                                                                 <  1:606658/150‑1 (MQ=255)
        gTTCCATTTATGCAGCATATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTa                                                                                                                                             >  2:1168144/1‑150 (MQ=255)
          tCCATATAAGCAGCATATTCACGTAGGCAATAAGCTGCTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTAcc                                                                                                                                           <  2:574545/150‑1 (MQ=255)
           ccATTTATGCAGCATATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTATGCGGGTGCTTACCa                                                                                                                                          >  1:810154/1‑150 (MQ=255)
           ccATTTATGCAGCATATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCa                                                                                                                                          >  1:1598831/1‑150 (MQ=255)
            cATTTATGCAGCATATTCACGTAGGCACTAATCTGGTTTTTATGGTGATCGTTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAg                                                                                                                                         <  2:2155527/150‑1 (MQ=255)
            cATTTATGCAGCATATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAg                                                                                                                                         <  2:796269/150‑1 (MQ=255)
            cATTTATGCAGCATATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCACCCGCTTACCAg                                                                                                                                         <  2:279695/150‑1 (MQ=255)
            cATTTATGCAGCATATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACATGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCat                                                                                                                                         >  1:1017594/1‑149 (MQ=255)
             aTTTATGCAGCATATTCACGTATGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCACCCGCTTACCAgg                                                                                                                                        <  2:325124/150‑1 (MQ=255)
             aTTTATGCAGCATATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAgg                                                                                                                                        <  2:209578/150‑1 (MQ=255)
             aTTTATGCAGCATATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCACCCGCTTACCAgg                                                                                                                                        <  1:2031488/150‑1 (MQ=255)
                 aTGCAGCATATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCAtt                                                                                                                                    >  2:368985/1‑150 (MQ=255)
                  tGCCGCATATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTTATTACCTGTTATTGATGCGTTGCCTGGTAAGCGGGTGCTTACCATGCAttt                                                                                                                                   >  1:181744/1‑150 (MQ=255)
                   gcagcaTATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGGTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCAtttt                                                                                                                                  <  1:2057322/150‑1 (MQ=255)
                      gcaTATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTaa                                                                                                                               >  1:1101889/1‑150 (MQ=255)
                       caTATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAAt                                                                                                                              <  2:46190/150‑1 (MQ=255)
                        atatTCACGTCGGCAATCAGCTGTTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTGCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCAATTTTAATg                                                                                                                             <  2:1523265/150‑1 (MQ=255)
                        atatTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATg                                                                                                                             <  1:1190020/150‑1 (MQ=255)
                         tatTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGc                                                                                                                            <  2:1745375/150‑1 (MQ=255)
                           ttCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGt                                                                                                                          <  1:1335821/150‑1 (MQ=255)
                             cACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTa                                                                                                                        >  2:1973948/1‑150 (MQ=255)
                             cACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTa                                                                                                                        >  1:2166307/1‑150 (MQ=255)
                             cACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTa                                                                                                                        >  1:152585/1‑150 (MQ=255)
                                gTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATgc                                                                                                                     <  1:1237753/150‑1 (MQ=255)
                                    gCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTa                                                                                                                 <  2:463426/150‑1 (MQ=255)
                                     cAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTac                                                                                                                <  1:571492/150‑1 (MQ=255)
                                     cAATAAGCTGGTTTTTCTGGTGATCGGGAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTac                                                                                                                <  1:584321/150‑1 (MQ=255)
                                           gCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACgca                                                                                                          >  1:17980/1‑150 (MQ=255)
                                              ggTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACgcagca                                                                                                       >  1:1985206/1‑150 (MQ=255)
                                                  tttCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATAcc                                                                                                   >  1:237344/1‑150 (MQ=255)
                                                   ttCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATAccc                                                                                                  >  2:1487494/1‑150 (MQ=255)
                                                       ggTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGtt                                                                                              <  2:1194715/150‑1 (MQ=255)
                                                       ggTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGtt                                                                                              >  2:686238/1‑150 (MQ=255)
                                                         tGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGtttt                                                                                            <  1:1036828/150‑1 (MQ=255)
                                                          gATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATTCGTTATGCGCTACGACGCAGCATACCCTGttttt                                                                                           >  2:1093854/1‑150 (MQ=255)
                                                            tCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTc                                                                                         >  2:1435850/1‑150 (MQ=255)
                                                             cGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCa                                                                                        >  2:896516/1‑150 (MQ=255)
                                                               gTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGc                                                                                      <  1:1761082/150‑1 (MQ=255)
                                                                tAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGcc                                                                                     >  2:717540/1‑150 (MQ=255)
                                                                   gCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCcaca                                                                                  >  1:1134029/1‑150 (MQ=255)
                                                                     gAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACAcc                                                                                <  1:185513/150‑1 (MQ=255)
                                                                      aaaTACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACca                                                                               >  1:2000847/1‑150 (MQ=255)
                                                                       aaTACCTGCGTCCTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACcag                                                                              >  2:862043/1‑150 (MQ=255)
                                                                        aTACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACcagc                                                                             <  1:1095825/150‑1 (MQ=255)
                                                                            cTGCGGCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGaa                                                                         <  1:1209094/150‑1 (MQ=255)
                                                                               cGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATgg                                                                      <  1:1973948/150‑1 (MQ=255)
                                                                                  cTTTCAGCAGTAAGGAGAGGTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGgaga                                                                   <  2:853653/150‑1 (MQ=255)
                                                                                    ttCAGCAGTACGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGGTGCCTTGTAAGCGTGTGCGTTCCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATg                                                                 <  1:1398070/150‑1 (MQ=255)
                                                                                    ttCAGCAGTAAGGAGAGTTTGTAGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATTCGTTATGCGCTACGACGCACCATACCCTGTTTTTTCAGCCACACCCGCAGAATCGAGATg                                                                 >  2:2042005/1‑150 (MQ=255)
                                                                                        gcagTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCg                                                             >  1:2042071/1‑150 (MQ=255)
                                                                                          agTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGcc                                                           >  2:1396524/1‑149 (MQ=255)
                                                                                          agTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCa                                                           >  2:935304/1‑150 (MQ=255)
                                                                                          agTAAGGAGAGTTTGTTGAACACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCa                                                           >  1:315551/1‑150 (MQ=255)
                                                                                              aGGAGAGTTTCAAGAGCAAGGTGAATACCAGTTCTTTATGCGTTTCCAGGAAAACGGGAGCTAACCAGGCATATATAATGCGTTATGCGCTACGACGAAGCAAACCCTGTTTAATCAGACACCCCAGCATAATGGAGATGGCCGCACGcc                                                       >  2:1193895/1‑149 (MQ=255)
                                                                                               ggAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCCGCAGAATGGAGATGGCCGCAGGCGt                                                      >  1:1797536/1‑150 (MQ=255)
                                                                                               ggAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGt                                                      >  1:1319212/1‑150 (MQ=255)
                                                                                               ggAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCAAACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGt                                                      >  1:1870729/1‑150 (MQ=255)
                                                                                                   agTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACg                                                  <  1:298406/150‑1 (MQ=255)
                                                                                                    gTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGc                                                 >  2:219919/1‑150 (MQ=255)
                                                                                                       tgttgAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAg                                              <  2:2166307/150‑1 (MQ=255)
                                                                                                        gttgAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGa                                             <  2:152585/150‑1 (MQ=255)
                                                                                                         ttgAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGaa                                            >  2:739341/1‑150 (MQ=255)
                                                                                                          tgAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGaaa                                           >  2:1746579/1‑150 (MQ=255)
                                                                                                             gCACTGTGAATACCGGTGCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTATAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCTAGAAATAc                                        <  2:1396975/150‑1 (MQ=255)
                                                                                                             gCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATAc                                        >  2:1880335/1‑150 (MQ=255)
                                                                                                                cGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGCCGCAGCATACCCTGTTTTTTCAGCCACCCCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATAcgcg                                     >  2:1104204/1‑150 (MQ=255)
                                                                                                                  gTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGaa                                   >  2:39375/1‑150 (MQ=255)
                                                                                                                    gcttACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGc                                 <  1:902879/148‑1 (MQ=255)
                                                                                                                       tACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTg                              >  2:443521/1‑150 (MQ=255)
                                                                                                                        aCCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATTAGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATTGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTgg                             >  1:733443/1‑150 (MQ=255)
                                                                                                                        aCCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTgg                             >  2:10708/1‑150 (MQ=255)
                                                                                                                          cTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATTCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGcc                           >  1:2016318/1‑150 (MQ=255)
                                                                                                                          cTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGcc                           <  1:852377/150‑1 (MQ=255)
                                                                                                                          cTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGcc                           <  1:1268133/150‑1 (MQ=255)
                                                                                                                          cTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGcc                           <  1:1181241/150‑1 (MQ=255)
                                                                                                                           tGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGCAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCg                          <  2:559031/150‑1 (MQ=255)
                                                                                                                             ttCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGAt                        <  1:686238/150‑1 (MQ=255)
                                                                                                                               cTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATTCGTTATGCGCTACGACGCAGCATACCCAGTTTTTTCAGCCACACCAGCAGAAGGGAGATGGCCGCAGGCCAGACCCCAGAAATACGCCAAGCTTGGCCGATag                      >  1:2058360/1‑150 (MQ=255)
                                                                                                                                ttGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATaga                     <  2:2000847/150‑1 (MQ=255)
                                                                                                                                  gATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAgg                   >  1:1332247/1‑150 (MQ=255)
                                                                                                                                  gATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAgg                   >  1:843536/1‑150 (MQ=255)
                                                                                                                                       gTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGt              >  2:1900090/1‑150 (MQ=255)
                                                                                                                                       gTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGt              <  1:22693/150‑1 (MQ=255)
                                                                                                                                        ttGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGtt             >  1:446388/1‑150 (MQ=255)
                                                                                                                                          gCCTGGTAAGCACCCGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTAAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTtg           >  2:2205781/1‑150 (MQ=255)
                                                                                                                                           ccTGGTAAGCACCCGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTtgt          >  2:634506/1‑150 (MQ=255)
                                                                                                                                           ccTGGTAAGCACCCGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTtgt          >  1:508096/1‑150 (MQ=255)
                                                                                                                                           ccTGGTAAGCACCCGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTtgt          >  2:812215/1‑150 (MQ=255)
                                                                                                                                           ccTGGTAAGCACCCGCTTACCAGGCATTTTTAATGCGTTATGCGATCCGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCGGATAGAGGCTGGTTtgt          >  2:290151/1‑150 (MQ=255)
                                                                                                                                            cTGGTAAGCACCCGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTtgtg         >  2:1868872/1‑150 (MQ=255)
                                                                                                                                              ggaaaGCGGGTGCTTACCAGGCATTTTTAATGCGGTATTCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACAGCAGCAGAATGGAGATGGCCGCAGGCGGGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGAt       <  1:368985/147‑1 (MQ=255)
                                                                                                                                               gTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATc      >  1:1451957/1‑150 (MQ=255)
                                                                                                                                               gTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATc      >  2:111120/1‑150 (MQ=255)
                                                                                                                                                tAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGTTTGGCCGATAGAGGCTGGTTTGTGATCg     >  1:420442/1‑150 (MQ=255)
                                                                                                                                                 aaGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATCGt    >  2:1230930/1‑150 (MQ=255)
                                                                                                                                                  aGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATCGtt   >  2:1652852/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TACGCTTTGTTCCATTTATGCAGCATATTCACGTAGGCAATAAGCTGGTTTTTCTGGTGATCGGTAAGCGAAATACCTGCGTCTTTCAGCAGTAAGGAGAGTTTGTTGAGCACGGTGATTACCTGTTCTTGATGCGTTGCCTGGTAAGCGGGTGCTTACCAGGCATTTTTAATGCGTTATGCGCTACGACGCAGCATACCCTGTTTTTTCAGCCACACCAGCAGAATGGAGATGGCCGCAGGCGTGACGCCAGAAATACGCGAAGCTTGGCCGATAGAGGCTGGTTTGTGATCGTTA  >  NC_000913/3923568‑3923864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: