Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,031,004 C→A 5.0% intergenic (‑33/‑157) fadB ← / → pepQ fused 3‑hydroxybutyryl‑CoA epimerase/delta(3)‑cis‑delta(2)‑trans‑enoyl‑CoA isomerase/enoyl‑CoA hydratase/3‑hydroxyacyl‑CoA dehydrogenase/proline dipeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,031,0040CA 5.0% 443.2 / 6.2 144intergenic (‑33/‑157)fadB/pepQfused 3‑hydroxybutyryl‑CoA epimerase/delta(3)‑cis‑delta(2)‑trans‑enoyl‑CoA isomerase/enoyl‑CoA hydratase/3‑hydroxyacyl‑CoA dehydrogenase/proline dipeptidase
Reads supporting (aligned to +/- strand):  ref base C (66/70);  new base A (3/5);  total (69/75)
Fisher's exact test for biased strand distribution p-value = 7.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 145 total aligned reads displayed.

AGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGG  >  NC_000913/4030856‑4031153
                                                                                                                                                    |                                                                                                                                                     
aGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCg                                                                                                                                                      >  2:1190929/1‑150 (MQ=255)
     ggCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCa                                                                                                                                                 <  1:1123321/150‑1 (MQ=255)
     ggCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCa                                                                                                                                                 <  1:1363694/150‑1 (MQ=255)
      gCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAg                                                                                                                                                <  2:1908382/150‑1 (MQ=255)
        gACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGAt                                                                                                                                              <  2:264416/150‑1 (MQ=255)
        gACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGAt                                                                                                                                              <  2:1244062/150‑1 (MQ=255)
        gACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGAt                                                                                                                                              <  1:139943/150‑1 (MQ=255)
         aCGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATg                                                                                                                                             <  1:1891610/150‑1 (MQ=255)
           ggTCGCAGGGGCGAGGTTATTCACTGAACCTGGGGCAGCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAg                                                                                                                                           <  1:1694323/150‑1 (MQ=255)
               gCAGTGTCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGa                                                                                                                                       >  1:2067741/1‑150 (MQ=255)
                cAGTGTCGAGTTTATTAACTTAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATATGTTCGTACCATATGAGTCGaa                                                                                                                                      >  2:1773801/1‑150 (MQ=255)
                   tgtCGAGTTTATTAACTGCACATTGGGCATCAAATACCAGTTCGGCAATGCCTTCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAgtg                                                                                                                                   <  1:361217/150‑1 (MQ=255)
                   tgtCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAgtg                                                                                                                                   <  2:2071077/150‑1 (MQ=255)
                   tgtCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAACATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAgtg                                                                                                                                   <  2:659319/150‑1 (MQ=255)
                    gtCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAgtgt                                                                                                                                  >  2:2019398/1‑150 (MQ=255)
                     tCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAgtgtg                                                                                                                                 >  2:1730778/1‑150 (MQ=255)
                      cGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAgtgtgt                                                                                                                                >  1:577205/1‑150 (MQ=255)
                        aGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTAt                                                                                                                              >  2:964460/1‑150 (MQ=255)
                            tattaACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCCGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGGCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTtg                                                                                                                          <  1:1108092/150‑1 (MQ=255)
                               taACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTgtgt                                                                                                                       >  1:1021482/1‑150 (MQ=255)
                                aaCTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGACTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGtt                                                                                                                      >  2:1336222/1‑150 (MQ=255)
                                aaCTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGACGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGtt                                                                                                                      >  1:1782484/1‑150 (MQ=255)
                                   tGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTaaa                                                                                                                   <  1:1619538/150‑1 (MQ=255)
                                      aCCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGCATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAat                                                                                                                >  2:1803918/1‑150 (MQ=255)
                                       ccTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCATTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAata                                                                                                               <  2:1650131/150‑1 (MQ=255)
                                         tGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATg                                                                                                             >  1:766299/1‑150 (MQ=255)
                                         tGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATg                                                                                                             <  2:2012841/150‑1 (MQ=255)
                                          ggggCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGc                                                                                                            >  2:2052183/1‑150 (MQ=255)
                                           gggCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGca                                                                                                           <  2:566249/150‑1 (MQ=255)
                                           gggCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGGACCAGATCAGTCGAAGTGTGTATTTTGGGTTAAAAATATGca                                                                                                           >  1:1057178/1‑150 (MQ=255)
                                           gggCATCAAATACCAGTTCGGCAATGCCAGCTTCCAGCCAGTCAAGGTACAGGGTGGCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGGCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGca                                                                                                           <  1:427080/150‑1 (MQ=255)
                                            ggCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACGGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGcaa                                                                                                          <  2:501340/150‑1 (MQ=255)
                                              cATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTTGAAGTGTGTATTTTGTGTTAAAAATATGcaaac                                                                                                        <  2:1882177/150‑1 (MQ=255)
                                                tCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGcaaacaa                                                                                                      >  2:119567/1‑150 (MQ=255)
                                                 cAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGcaaacaaa                                                                                                     >  1:2174001/1‑150 (MQ=255)
                                                     tACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGAt                                                                                                 >  1:620338/1‑150 (MQ=255)
                                                      aCCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGAtt                                                                                                <  2:1155422/150‑1 (MQ=255)
                                                       ccAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTa                                                                                               >  1:788919/1‑150 (MQ=255)
                                                       ccAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTa                                                                                               >  1:781050/1‑150 (MQ=255)
                                                              ggCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAAt                                                                                        <  2:2212962/150‑1 (MQ=255)
                                                                   tGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCAGCAAGTTGATCTGGTCCTACAAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGa                                                                                   >  2:364575/1‑150 (MQ=255)
                                                                     ccATCTTCCAGCCAGTCAAGGTACAGGGTGTCGGCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTTTTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATc                                                                                 <  2:1323659/150‑1 (MQ=255)
                                                                      cATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGCGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCt                                                                                <  1:664904/150‑1 (MQ=255)
                                                                         cTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGAt                                                                             <  1:74275/150‑1 (MQ=255)
                                                                         cTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGAt                                                                             >  1:2032129/1‑150 (MQ=255)
                                                                          ttCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTATGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATc                                                                            <  2:1021482/150‑1 (MQ=255)
                                                                             cagccagTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACg                                                                         >  1:891026/1‑150 (MQ=255)
                                                                             cagccagTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGGCGAAGTGTGTATTTTGTGTTAAAAATATGCAACCAAAAGATTAAAGAAATGCCGATCTGCTCACg                                                                         >  2:1477514/1‑150 (MQ=255)
                                                                             cagacagGCAAGGTACAGTGTGTCTCCTTTTTAAAGCATTTCAGTCTCCTTAATCCGCAAGTTGATCTGTTATTACCATATGATTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCAct                                                                         <  1:449581/150‑2 (MQ=255)
                                                                              agccagTCAAGGGACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAAGAAAAGATTAAAGAAATGCCGATCTGATCACGc                                                                        <  1:350202/150‑1 (MQ=255)
                                                                                 cagTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCg                                                                     <  2:1269674/150‑1 (MQ=255)
                                                                                 cagTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCg                                                                     <  2:845041/150‑1 (MQ=255)
                                                                                     cAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAg                                                                 <  2:626540/150‑1 (MQ=255)
                                                                                          tACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATTCCGATCTGATCACGCTCGGCAGAAATc                                                            >  1:992569/1‑150 (MQ=255)
                                                                                           aCAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCa                                                           >  2:491510/1‑150 (MQ=255)
                                                                                           aCAGGGTGTCGCCTTTGTAAAGAATGTCAGTCTCCTGAATCCGAAAGGTGATCTGGGCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCa                                                           <  2:186751/150‑1 (MQ=255)
                                                                                            cAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCAc                                                          <  1:1783523/150‑1 (MQ=255)
                                                                                              gggTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGc                                                        >  2:620530/1‑150 (MQ=255)
                                                                                              gggTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGc                                                        <  2:1083612/150‑1 (MQ=255)
                                                                                                gtgtCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGGTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGctc                                                      <  2:972706/150‑1 (MQ=255)
                                                                                                  gtCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTg                                                    <  1:1190929/150‑1 (MQ=255)
                                                                                                   tCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTgg                                                   <  2:181273/150‑1 (MQ=255)
                                                                                                    cGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGa                                                  >  1:2103079/1‑150 (MQ=255)
                                                                                                     gCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGAt                                                 >  1:1437415/1‑150 (MQ=255)
                                                                                                     gCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGAt                                                 >  1:1612524/1‑150 (MQ=255)
                                                                                                      ccTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGGATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATg                                                >  1:1327630/1‑150 (MQ=255)
                                                                                                         ttGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAAc                                             <  1:2093641/150‑1 (MQ=255)
                                                                                                          tGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCGGATCTGATCACGGTCGGAAGAAATCACGATCTGTATGAAca                                            >  1:80221/1‑149 (MQ=255)
                                                                                                          tGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACg                                            <  2:1399466/150‑1 (MQ=255)
                                                                                                            tAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGAt                                          >  1:1181641/1‑150 (MQ=255)
                                                                                                             aaaGCATGTCAGTCTCCTGAATCCGCTAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGAtg                                         <  1:1091592/150‑1 (MQ=255)
                                                                                                             aaaGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATTCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGAtg                                         >  2:1130268/1‑150 (MQ=255)
                                                                                                               aGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGAtgtg                                       <  1:673826/150‑1 (MQ=255)
                                                                                                                 cATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTTGATGAACGATGTGCt                                     <  2:1517221/150‑1 (MQ=255)
                                                                                                                 cATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCt                                     <  1:261004/150‑1 (MQ=255)
                                                                                                                  aTGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTa                                    <  2:399466/150‑1 (MQ=255)
                                                                                                                    gTCAGTCTCCTGAATCCGCAAGTGGATCTGGCCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAATCAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAAGCACGCTCTGTATGAACGATGTGCTAAg                                  <  2:875516/150‑1 (MQ=255)
                                                                                                                    gTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAg                                  >  1:700829/1‑150 (MQ=255)
                                                                                                                    gTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAg                                  <  2:766299/150‑1 (MQ=255)
                                                                                                                     tCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATTAACGATGTGCTAAGa                                 <  1:1708989/150‑1 (MQ=255)
                                                                                                                     tCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGa                                 <  2:1958619/150‑1 (MQ=255)
                                                                                                                     tCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGa                                 <  1:770122/150‑1 (MQ=255)
                                                                                                                       aGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGCGGTGCTGAGATg                               >  2:1592402/1‑150 (MQ=255)
                                                                                                                        gTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATACCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATTTGCTAAGATGc                              >  1:363700/1‑150 (MQ=255)
                                                                                                                          ctcCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGAATAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGTATGAACGATTTGCTAAGATTCgg                            >  1:1964692/1‑150 (MQ=255)
                                                                                                                           tcCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGga                           >  2:235608/1‑150 (MQ=255)
                                                                                                                            ccTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGgag                          >  1:841679/1‑150 (MQ=255)
                                                                                                                             cTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGgaga                         >  2:1214166/1‑150 (MQ=255)
                                                                                                                              tGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGAc                        >  2:57014/1‑150 (MQ=255)
                                                                                                                                 aTCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGATGTGTGTATTTTGTGGTAAAAATATGCAAACAAAAGATTAAAGAAATGCCCAACTGATCACGCTCGGCAGCAATCAAGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTa                     >  2:1380144/1‑150 (MQ=255)
                                                                                                                                    cGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAgg                  >  1:2173501/1‑150 (MQ=255)
                                                                                                                                    cGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAgg                  >  2:723100/1‑150 (MQ=255)
                                                                                                                                         actcaTCTGGTACGACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCaaa             >  2:1069222/5‑150 (MQ=255)
                                                                                                                                              tCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAAc        <  2:791312/150‑1 (MQ=255)
                                                                                                                                              tCTGGGCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAAc        <  1:1478587/150‑1 (MQ=255)
                                                                                                                                                tGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAg      <  2:2006597/150‑1 (MQ=255)
                                                                                                                                                 ggTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGa     >  1:1930167/1‑150 (MQ=255)
                                                                                                                                                 ggTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGa     <  1:288700/150‑1 (MQ=255)
                                                                                                                                                  gTCGTACCATATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGaa    <  2:690739/150‑1 (MQ=255)
                                                                                                                                                  gTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGAAGGACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGaa    >  2:1394145/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
AGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGG  >  NC_000913/4030856‑4031153

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: