Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,031,006 T→G 5.0% intergenic (‑35/‑155) fadB ← / → pepQ fused 3‑hydroxybutyryl‑CoA epimerase/delta(3)‑cis‑delta(2)‑trans‑enoyl‑CoA isomerase/enoyl‑CoA hydratase/3‑hydroxyacyl‑CoA dehydrogenase/proline dipeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,031,0060TG 5.0% 436.3 / 8.5 145intergenic (‑35/‑155)fadB/pepQfused 3‑hydroxybutyryl‑CoA epimerase/delta(3)‑cis‑delta(2)‑trans‑enoyl‑CoA isomerase/enoyl‑CoA hydratase/3‑hydroxyacyl‑CoA dehydrogenase/proline dipeptidase
Reads supporting (aligned to +/- strand):  ref base T (65/72);  new base G (5/3);  total (70/75)
Fisher's exact test for biased strand distribution p-value = 4.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.39e-01

Only 100 of 145 total aligned reads displayed.

GGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGG  >  NC_000913/4030861‑4031154
                                                                                                                                                 |                                                                                                                                                    
ggCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCa                                                                                                                                                  <  1:1123321/150‑1 (MQ=255)
ggCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCa                                                                                                                                                  <  1:1363694/150‑1 (MQ=255)
 gCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAg                                                                                                                                                 <  2:1908382/150‑1 (MQ=255)
   gACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGAt                                                                                                                                               <  2:264416/150‑1 (MQ=255)
   gACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGAt                                                                                                                                               <  1:139943/150‑1 (MQ=255)
    aCGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATg                                                                                                                                              <  1:1891610/150‑1 (MQ=255)
     cGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTACGACCAGAtca                                                                                                                                             <  2:1786642/150‑3 (MQ=255)
      ggTCGCAGGGGCGAGGTTATTCACTGAACCTGGGGCAGCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAg                                                                                                                                            <  1:1694323/150‑1 (MQ=255)
          gCAGTGTCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTGGa                                                                                                                                        >  2:441282/1‑150 (MQ=255)
          gCAGTGTCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGa                                                                                                                                        >  1:2067741/1‑150 (MQ=255)
           cAGTGTCGAGTTTATTAACTTAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATATGTTCGTACCATATGAGTCGaa                                                                                                                                       >  2:1773801/1‑150 (MQ=255)
              tgtCGAGTTTATTAACTGCACATTGGGCATCAAATACCAGTTCGGCAATGCCTTCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAgtg                                                                                                                                    <  1:361217/150‑1 (MQ=255)
              tgtCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAgtg                                                                                                                                    <  2:2071077/150‑1 (MQ=255)
              tgtCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAACATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAgtg                                                                                                                                    <  2:659319/150‑1 (MQ=255)
               gtCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAgtgt                                                                                                                                   >  2:2019398/1‑150 (MQ=255)
                tCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAgtgtg                                                                                                                                  >  2:1237491/1‑150 (MQ=255)
                 cGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAgtgtgt                                                                                                                                 >  1:577205/1‑150 (MQ=255)
                  gAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTa                                                                                                                                <  2:1873081/150‑1 (MQ=255)
                   aGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTAt                                                                                                                               >  2:964460/1‑150 (MQ=255)
                       tattaACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCCGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGGCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTtg                                                                                                                           <  1:1108092/150‑1 (MQ=255)
                       tattaACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTtg                                                                                                                           >  2:2008202/1‑150 (MQ=255)
                        attaACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCTCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGAGATCTGGTCGTACCAGATGAGTCGAAGTATGTATTTtgt                                                                                                                          >  2:1942467/1‑150 (MQ=255)
                        attaACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTtgt                                                                                                                          >  2:1318663/1‑150 (MQ=255)
                          taACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTgtgt                                                                                                                        >  1:1021482/1‑150 (MQ=255)
                           aaCTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGACTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGtt                                                                                                                       >  2:1336222/1‑150 (MQ=255)
                           aaCTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGACGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGtt                                                                                                                       >  1:1782484/1‑150 (MQ=255)
                              tGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTaaa                                                                                                                    <  1:1619538/150‑1 (MQ=255)
                                  ccTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCATTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAata                                                                                                                <  2:1650131/150‑1 (MQ=255)
                                    tGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATg                                                                                                              >  1:766299/1‑150 (MQ=255)
                                      gggCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGGACCAGATCAGTCGAAGTGTGTATTTTGGGTTAAAAATATGca                                                                                                            >  1:1057178/1‑150 (MQ=255)
                                      gggCATCAAATACCAGTTCGGCAATGCCAGCTTCCAGCCAGTCAAGGTACAGGGTGGCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGGCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGca                                                                                                            <  1:427080/150‑1 (MQ=255)
                                       ggCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACGGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGcaa                                                                                                           <  2:501340/150‑1 (MQ=255)
                                          aTCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGcaaaca                                                                                                        <  2:836312/150‑1 (MQ=255)
                                            cAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGcaaacaaa                                                                                                      >  1:2174001/1‑150 (MQ=255)
                                                tACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGAt                                                                                                  >  1:620338/1‑150 (MQ=255)
                                                 aCCAGTTCGGGAATGCCATCTTCGATCCAGTCAAGGTACAGGTTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGAtt                                                                                                 <  2:2036038/150‑1 (MQ=255)
                                                 aCCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGAtt                                                                                                 <  2:1155422/150‑1 (MQ=255)
                                                  ccAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTa                                                                                                >  1:781050/1‑150 (MQ=255)
                                                  ccAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTa                                                                                                >  1:788919/1‑150 (MQ=255)
                                                           ctaTGCCATCTTCCAGCCAGGCAAGGTACAGGGTGTCACCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGGCGTACCAGATGAGTCGAAGTGTGGATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGc                                                                                       <  2:1543169/148‑1 (MQ=255)
                                                                ccATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATc                                                                                  <  2:1430737/150‑1 (MQ=255)
                                                                 cATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGCGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCt                                                                                 <  1:664904/150‑1 (MQ=255)
                                                                    cTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGAt                                                                              <  1:74275/150‑1 (MQ=255)
                                                                    cTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGAt                                                                              >  1:2032129/1‑150 (MQ=255)
                                                                     ttCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTATGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATc                                                                             <  2:1021482/150‑1 (MQ=255)
                                                                        cagccagTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACg                                                                          >  1:891026/1‑150 (MQ=255)
                                                                        cagacagGCAAGGTACAGTGTGTCTCCTTTTTAAAGCATTTCAGTCTCCTTAATCCGCAAGTTGATCTGTTATTACCATATGATTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCAct                                                                          <  1:449581/150‑2 (MQ=255)
                                                                         agccagTCAAGGGACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAAGAAAAGATTAAAGAAATGCCGATCTGATCACGc                                                                         <  1:350202/150‑1 (MQ=255)
                                                                            cagTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCg                                                                      <  2:1269674/150‑1 (MQ=255)
                                                                                cAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAg                                                                  <  2:626540/150‑1 (MQ=255)
                                                                                     tACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATTCCGATCTGATCACGCTCGGCAGAAATc                                                             >  1:992569/1‑150 (MQ=255)
                                                                                      aCAGGGTGTCGCCTTTGTAAAGAATGTCAGTCTCCTGAATCCGAAAGGTGATCTGGGCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCa                                                            <  2:186751/150‑1 (MQ=255)
                                                                                       cAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCAc                                                           <  1:1783523/150‑1 (MQ=255)
                                                                                         gggTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGc                                                         <  2:1083612/150‑1 (MQ=255)
                                                                                           gtgtCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTCCCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCGGATCACGATCGGCAGAAATCACGctc                                                       >  2:2085196/1‑150 (MQ=255)
                                                                                           gtgtCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGctc                                                       <  2:1712518/150‑1 (MQ=255)
                                                                                           gtgtCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGGTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGctc                                                       <  2:972706/150‑1 (MQ=255)
                                                                                             gtCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTg                                                     <  1:1190929/150‑1 (MQ=255)
                                                                                              tCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTgg                                                    <  2:181273/150‑1 (MQ=255)
                                                                                              gcGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTgg                                                    <  2:509801/149‑1 (MQ=255)
                                                                                               cGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGa                                                   >  1:2103079/1‑150 (MQ=255)
                                                                                                gCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGAt                                                  >  1:1612524/1‑150 (MQ=255)
                                                                                                gCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGAt                                                  >  1:1437415/1‑150 (MQ=255)
                                                                                                 ccTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGGATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATg                                                 >  1:1327630/1‑150 (MQ=255)
                                                                                                    ttGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAAc                                              <  1:2093641/150‑1 (MQ=255)
                                                                                                     tGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCGGATCTGATCACGGTCGGAAGAAATCACGATCTGTATGAAca                                             >  1:80221/1‑149 (MQ=255)
                                                                                                     tGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACg                                             <  2:1399466/150‑1 (MQ=255)
                                                                                                       tAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGAt                                           >  1:1181641/1‑150 (MQ=255)
                                                                                                        aaaGCATGTCAGTCTCCTGAATCCGCTAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGAtg                                          <  1:1091592/150‑1 (MQ=255)
                                                                                                        aaaGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATTCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGAtg                                          >  2:1130268/1‑150 (MQ=255)
                                                                                                          aGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGAtgtg                                        <  1:673826/150‑1 (MQ=255)
                                                                                                            cATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTTGATGAACGATGTGCt                                      <  2:1517221/150‑1 (MQ=255)
                                                                                                            cATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCt                                      <  1:261004/150‑1 (MQ=255)
                                                                                                             aTGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTa                                     <  2:399466/150‑1 (MQ=255)
                                                                                                               gTCAGTCTCCTGAATCCGCAAGTGGATCTGGCCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAATCAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAAGCACGCTCTGTATGAACGATGTGCTAAg                                   <  2:875516/150‑1 (MQ=255)
                                                                                                               gTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAg                                   >  1:700829/1‑150 (MQ=255)
                                                                                                                tCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATTAACGATGTGCTAAGa                                  <  1:1708989/150‑1 (MQ=255)
                                                                                                                tCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGa                                  <  1:770122/150‑1 (MQ=255)
                                                                                                                   gTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATACCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATTTGCTAAGATGc                               >  1:363700/1‑150 (MQ=255)
                                                                                                                   gTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAAAATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACTCTCGGCATAAATCACGCTCTGTATGAACGATGAGCTAAGATGc                               >  2:1351900/1‑150 (MQ=255)
                                                                                                                     ctcCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGAATAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGTATGAACGATTTGCTAAGATTCgg                             >  1:1964692/1‑150 (MQ=255)
                                                                                                                      tcCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGga                            >  2:235608/1‑150 (MQ=255)
                                                                                                                       ccTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGgag                           >  1:841679/1‑150 (MQ=255)
                                                                                                                        cTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGgaga                          >  2:1214166/1‑150 (MQ=255)
                                                                                                                         tGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGAc                         >  2:57014/1‑150 (MQ=255)
                                                                                                                          gAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAATAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGCCAGAAATCACGCTCAGGATGCACGATGTGCTAAGATGCGGAGACt                        >  2:1192930/1‑150 (MQ=255)
                                                                                                                           aaTCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACtt                       >  2:743025/1‑150 (MQ=255)
                                                                                                                               cGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAACTGCCCATCTGATCACGCTCTGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAgg                   <  2:277121/150‑1 (MQ=255)
                                                                                                                               cGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAgg                   >  2:723100/1‑150 (MQ=255)
                                                                                                                               cGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAgg                   >  1:2173501/1‑150 (MQ=255)
                                                                                                                                 tcgactcaTCTGGTACGACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTc                 >  2:665385/8‑150 (MQ=255)
                                                                                                                                    actcaTCTGGTACGACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCaaa              >  2:1069222/5‑150 (MQ=255)
                                                                                                                                         tCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAAc         <  2:791312/150‑1 (MQ=255)
                                                                                                                                         tCTGGGCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAAc         <  1:1478587/150‑1 (MQ=255)
                                                                                                                                           tGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAg       <  2:2006597/150‑1 (MQ=255)
                                                                                                                                            ggTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGa      >  1:1930167/1‑150 (MQ=255)
                                                                                                                                            ggTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGa      <  1:288700/150‑1 (MQ=255)
                                                                                                                                             gTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGAAGGACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGaa     >  2:1394145/1‑150 (MQ=255)
                                                                                                                                               cGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAgg   >  2:592544/1‑150 (MQ=255)
                                                                                                                                                gTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAggg  >  2:885140/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
GGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCCGCAAGGTGATCTGGTCGTACCAGATGAGTCGAAGTGTGTATTTTGTGTTAAAAATATGCAAACAAAAGATTAAAGAAATGCCGATCTGATCACGCTCGGCAGAAATCACGCTCTGGATGAACGATGTGCTAAGATGCGGAGACTTAAGGTCAAAAAAACAGAAGGG  >  NC_000913/4030861‑4031154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: