Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,121,622 T→A 5.1% E42D (GAA→GAT hslU ← molecular chaperone and ATPase component of HslUV protease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,121,6220TA 5.1% 361.5 / 4.3 124E42D (GAA→GAThslUmolecular chaperone and ATPase component of HslUV protease
Reads supporting (aligned to +/- strand):  ref base T (58/59);  new base A (4/3);  total (62/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 126 total aligned reads displayed.

TAGCCCACTTCGGTGAATTTGGTCGCTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGACATGGGAGATCCTTACGCTTTGTAGCT  >  NC_000913/4121476‑4121771
                                                                                                                                                  |                                                                                                                                                     
tAGCCCACTTCGGTGAATTTGGTCGCTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCg                                                                                                                                                    <  1:1548350/150‑1 (MQ=255)
 aGCCCACTTCGGTGAATTTGGTCGCTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGt                                                                                                                                                   >  1:940570/1‑150 (MQ=255)
  ccccACTTCGGTGAATTTGGTCGCTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGtt                                                                                                                                                  <  1:1162553/149‑1 (MQ=255)
   cccACTTCTGTGAATTTGGTCGCTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTg                                                                                                                                                 <  2:391254/150‑1 (MQ=255)
   cccACTTCGGTGAATTTGGTCGCTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTg                                                                                                                                                 >  2:2061293/1‑150 (MQ=255)
   cccACTTCGGTGAATTTGGTCGCTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTg                                                                                                                                                 >  1:313890/1‑150 (MQ=255)
     cACTTCGGTGAATTTGGTCGCTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAg                                                                                                                                               <  1:298951/150‑1 (MQ=255)
         tCGGTGAATTTGGTCGCTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCAACGAAGAGCTGc                                                                                                                                           <  1:976477/150‑1 (MQ=255)
          cGGTGAAATAGGTCGCATCAACTTTGATGAACGGCCCATTCGCCAGCTTAGCCAGACGACGCGCGATTTCAGATATACCGACCCCGGTCGGGCCGATCATCAGGATATTATTCGTGGTCACTTAATGGCGCAGCTCTTCGTTGATCTGCa                                                                                                                                          >  2:1411902/1‑150 (MQ=255)
            gTGAATTTGGTCGCTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATg                                                                                                                                        >  2:129004/1‑150 (MQ=255)
            gTGAATTTGGTCGCTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATg                                                                                                                                        <  1:1495980/150‑1 (MQ=255)
              gAATTTGGTCGCTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGcc                                                                                                                                      >  2:978654/1‑149 (MQ=255)
              gAATTTGGTCGCTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCg                                                                                                                                      <  1:12576/150‑1 (MQ=255)
                aTTTGGTCGCTTCAACTTTGATGAACGGCGCATTCTCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGATCTTCGTTGAGCTGCATGCGAc                                                                                                                                    >  2:1935683/1‑150 (MQ=255)
                aTTTGGTCGCTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGTTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGAc                                                                                                                                    >  1:127269/1‑150 (MQ=255)
                aTTTGGTCGCTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGGCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGAc                                                                                                                                    <  2:1162397/150‑1 (MQ=255)
                      tCGCTTCAACATTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGCCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGc                                                                                                                              <  1:565300/150‑1 (MQ=255)
                       cGCTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCg                                                                                                                             <  1:865398/150‑1 (MQ=255)
                        gCTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGTCGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCgg                                                                                                                            <  1:2208431/150‑1 (MQ=255)
                        gCTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCgg                                                                                                                            <  1:895083/150‑1 (MQ=255)
                             aaCTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCCGCTCTTCGTTGAGCTGCCTGCGACGCCCGCGGTTACg                                                                                                                       >  2:391995/1‑150 (MQ=255)
                              agtttGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGc                                                                                                                      <  1:601921/148‑1 (MQ=255)
                              aCTTTGATCAACGCCGAATAAGCCAGCTTAGCCAGACCACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGGCACTTCATGGGGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGc                                                                                                                      <  2:1073974/150‑1 (MQ=255)
                               cTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCa                                                                                                                     >  2:1365529/1‑150 (MQ=255)
                                tttGATGAACGGCGCATGCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCag                                                                                                                    <  1:950453/150‑1 (MQ=255)
                                  tgatgaACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCagag                                                                                                                  <  2:2105327/150‑1 (MQ=255)
                                  tgatgaACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCagag                                                                                                                  >  2:1619494/1‑150 (MQ=255)
                                   gatgaACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGc                                                                                                                 >  1:68288/1‑150 (MQ=255)
                                    atgaACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCATTGAGCTGCATGCGACGCCAGCGGTTCCGCAGAGCa                                                                                                                >  2:1837584/1‑150 (MQ=255)
                                     aagctggCGAATGCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCaa                                                                                                               >  2:410009/6‑150 (MQ=255)
                                      gaACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAAt                                                                                                              >  1:506765/1‑150 (MQ=255)
                                        aCGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGCGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCg                                                                                                            >  1:1844227/1‑150 (MQ=255)
                                           gcgcATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCca                                                                                                         <  2:1005777/150‑1 (MQ=255)
                                           gcgcATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCca                                                                                                         >  2:832550/1‑150 (MQ=255)
                                             gcATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCcaca                                                                                                       <  1:2183232/150‑1 (MQ=255)
                                              cATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAg                                                                                                      <  1:1890114/150‑1 (MQ=255)
                                                 tCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAAc                                                                                                   >  2:1254922/1‑150 (MQ=255)
                                                  cGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACg                                                                                                  >  1:1507554/1‑150 (MQ=255)
                                                   gCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGc                                                                                                 >  1:1767897/1‑150 (MQ=255)
                                                     cAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCtt                                                                                               >  2:730700/1‑150 (MQ=255)
                                                      aGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTg                                                                                              <  1:1183698/150‑1 (MQ=255)
                                                        cTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGc                                                                                            <  2:186596/150‑1 (MQ=255)
                                                            gCCAGACGACGGGCGATTTCAGTTTTACCGACTCAGGTCGGGCCGAACATCAGGATATATTTCGGGGTCACTTCATGGCGCAGCTCTTCGATGAGCTCCATGCGAAGCCAGCGGATACGCAGAGCAATCGCCACAGATCGCTTGGCGTTg                                                                                        >  1:375424/1‑150 (MQ=255)
                                                            gCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTg                                                                                        <  2:1165124/150‑1 (MQ=255)
                                                            gCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCGCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGttt                                                                                        >  2:589692/1‑149 (MQ=255)
                                                            cccAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTg                                                                                        <  2:1845174/149‑1 (MQ=255)
                                                              cAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTc                                                                                      >  1:891049/1‑150 (MQ=255)
                                                                   gacgGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGc                                                                                 <  1:1872321/150‑1 (MQ=255)
                                                                   gacgGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGc                                                                                 >  2:1620874/1‑150 (MQ=255)
                                                                    acgGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGcc                                                                                <  1:827596/150‑1 (MQ=255)
                                                                    acgGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGcc                                                                                <  2:732052/150‑1 (MQ=255)
                                                                      gggCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCGCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCga                                                                              <  2:974630/150‑1 (MQ=255)
                                                                        gCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCgatg                                                                            <  2:1587942/150‑1 (MQ=255)
                                                                        gCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCgatg                                                                            <  2:1459631/150‑1 (MQ=255)
                                                                         cGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCgatga                                                                           >  1:464646/1‑150 (MQ=255)
                                                                                 gTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTa                                                                   >  2:711017/1‑150 (MQ=255)
                                                                                   tttCCCGACACCGGGCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCGCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATc                                                                 <  1:1335222/150‑1 (MQ=255)
                                                                                     tACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCa                                                               >  1:2189823/1‑150 (MQ=255)
                                                                                      aCCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAg                                                              >  1:836095/1‑150 (MQ=255)
                                                                                         gACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTc                                                           <  1:1246002/150‑1 (MQ=255)
                                                                                            acCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCCCTTCATGGCGCAGCTCTTCGTTGCGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGGTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCt                                                        >  2:1077385/1‑150 (MQ=255)
                                                                                            acCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCt                                                        <  2:595479/150‑1 (MQ=255)
                                                                                             ccGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTATAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTg                                                       <  2:639228/150‑1 (MQ=255)
                                                                                                gTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTGCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACg                                                    <  1:1541325/150‑1 (MQ=255)
                                                                                                    ggCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGTAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGAttt                                                >  2:1391581/1‑150 (MQ=255)
                                                                                                    ggCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGAttt                                                >  1:1460131/1‑150 (MQ=255)
                                                                                                    ggCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGAttt                                                >  1:382869/1‑150 (MQ=255)
                                                                                                       cGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTcgc                                             >  1:564492/1‑150 (MQ=255)
                                                                                                           catcaGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTgg                                         >  1:1688034/1‑150 (MQ=255)
                                                                                                            atcaGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTggg                                        >  2:1974900/1‑150 (MQ=255)
                                                                                                             tcaGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTgggg                                       <  2:583521/150‑1 (MQ=255)
                                                                                                             tcaGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCCGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTgggg                                       >  2:1782147/1‑150 (MQ=255)
                                                                                                                    atTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGATGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCAGACGATTTCGCCTGGGGTCATTTc                                >  2:536980/1‑150 (MQ=255)
                                                                                                                    aattttCGGGGTCACATCAAGGCGCAGCTCTTCGTTTAGCTGCAGGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTc                                <  2:381858/148‑1 (MQ=255)
                                                                                                                     tttttCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGATCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCa                               <  1:743108/150‑1 (MQ=255)
                                                                                                                      ttttCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAg                              <  1:70415/150‑1 (MQ=255)
                                                                                                                      ttttCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGAGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAg                              >  1:888144/1‑150 (MQ=255)
                                                                                                                       tttCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAAGCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGa                             <  1:1770818/150‑1 (MQ=255)
                                                                                                                        ttCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGTCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGAc                            <  1:978654/150‑1 (MQ=255)
                                                                                                                          cGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGACAt                          >  2:957669/1‑150 (MQ=255)
                                                                                                                           ggggTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGACATg                         >  1:82690/1‑150 (MQ=255)
                                                                                                                           ggggTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGACATg                         >  2:13799/1‑150 (MQ=255)
                                                                                                                            gggTCACTTCATGGCGCAGCTCTTCGTTGCGCTGCATGCGACGCCAGCGGTAACGCAGAGCAATCGCCACAGAACGCTTGGCGTTCTCCTGGCCGATGATGTGCTGATCCAGTTCGCTGACGAATACGCGTGGGGTCAGTTCAGACATgg                        >  1:1060621/1‑150 (MQ=255)
                                                                                                                            gggTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCGTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGACATgg                        <  1:129004/150‑1 (MQ=255)
                                                                                                                              ggcACTTCATGTCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCGACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGTTTTCGCGTGGGTTCATTTCAGACATGGga                      <  1:1411902/148‑1 (MQ=255)
                                                                                                                               tCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCCGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGACATGGgag                     <  1:410009/150‑1 (MQ=255)
                                                                                                                               tCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGACATGGgag                     <  1:1912033/150‑1 (MQ=255)
                                                                                                                               gcACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGACATGGgag                     <  2:1466927/149‑1 (MQ=255)
                                                                                                                                   ttCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGACATGGGAGATcc                 <  2:506765/150‑1 (MQ=255)
                                                                                                                                    tCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGACATGGGAGATCCt                >  2:526754/1‑150 (MQ=255)
                                                                                                                                    tCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGACATGGGAGATCCt                <  1:1432755/150‑1 (MQ=255)
                                                                                                                                     tcgcatgcAGCTCAACGAAGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGCCGATTTCGCGTGGGGTCATTTCAGACATGGGAGATCCtt               >  1:662957/7‑150 (MQ=255)
                                                                                                                                     tcgcatgcAGCTCAACGAAGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGACATGGGAGATCCtt               >  2:497992/7‑150 (MQ=255)
                                                                                                                                       gcatgcAGCTCAACGAAGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGACATGGGAGATCCTTAc             >  1:1757477/5‑150 (MQ=255)
                                                                                                                                        catgcAGCTCAACGAAGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGACATGGGAGATCCTTACg            >  1:2069537/4‑150 (MQ=255)
                                                                                                                                          cgcAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGACATGGGAGATCCTTACGCt          >  2:108325/1‑150 (MQ=255)
                                                                                                                                            cAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCGACAGAAGGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCTGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGACATGGGAGATCCTTACGCttt        <  1:317514/150‑1 (MQ=255)
                                                                                                                                              gCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGACATGGGAGATCCTTACGCTTTGt      <  2:1463435/150‑1 (MQ=255)
                                                                                                                                                 ctTCGTGGAGCTGCATGCGACGCCAGCGGGTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGACATGGGAGATCCTTACGCTTTGTAGc   <  1:1139520/150‑1 (MQ=255)
                                                                                                                                                  ttCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGACATGGGAGATCCTTACGCTTTGTAGCt  <  2:1507554/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
TAGCCCACTTCGGTGAATTTGGTCGCTTCAACTTTGATGAACGGCGCATTCGCCAGCTTAGCCAGACGACGGGCGATTTCAGTTTTACCGACACCGGTCGGGCCGATCATCAGGATATTTTTCGGGGTCACTTCATGGCGCAGCTCTTCGTTGAGCTGCATGCGACGCCAGCGGTTACGCAGAGCAATCGCCACAGAACGCTTGGCGTTGTCCTGGCCGATGATGTGCTTATCCAGTTCGCTGACGATTTCGCGTGGGGTCATTTCAGACATGGGAGATCCTTACGCTTTGTAGCT  >  NC_000913/4121476‑4121771

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: