Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 454,853 T→C 5.5% intergenic (+64/‑280) bolA → / → tig stationary‑phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC/peptidyl‑prolyl cis/trans isomerase (trigger factor)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913454,8530TC 5.5% 391.7 / 9.2 129intergenic (+64/‑280)bolA/tigstationary‑phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC/peptidyl‑prolyl cis/trans isomerase (trigger factor)
Reads supporting (aligned to +/- strand):  ref base T (62/59);  new base C (4/3);  total (67/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.88e-01

Only 100 of 129 total aligned reads displayed.

TACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTT  >  NC_000913/454705‑455001
                                                                                                                                                    |                                                                                                                                                    
   ttACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGCTTTTATGAAAAACGGCCTGCgg                                                                                                                                                  >  1:137728/1‑150 (MQ=255)
    tACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCggg                                                                                                                                                 >  2:1899296/1‑150 (MQ=255)
     acacTATAAAGGAGTTGGAAGGCTTGCAGGACACCGTCTTTTCCTCACCTCCCTGTCGTGGAGCATGACGCATCGCGTAAATACGCATTTTCAACTGTCGGCGCTTTTCCAGTATGTTGCTAATGATTTAATGAAAAACGGGCTGCggtc                                                                                                                                                <  2:472634/150‑3 (MQ=255)
      cacTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGcc                                                                                                                                               >  2:675070/1‑150 (MQ=255)
        cTATTAATGAGTGGGAAGTGATGCATGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCTTGAAGCAGCGGCTAACAACGCATTTGCAACTGTCTTCGCATTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGCCCTGCGGGCCgt                                                                                                                                             <  2:1740660/150‑1 (MQ=255)
            tAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCgttttg                                                                                                                                         <  2:790111/150‑1 (MQ=255)
             aaGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCgttttgt                                                                                                                                        <  1:1814152/150‑1 (MQ=255)
              cggAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCCGCAGGCCgtttttca                                                                                                                                       <  1:2198575/149‑4 (MQ=255)
               ggAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCgttttgttt                                                                                                                                      >  2:1444643/1‑150 (MQ=255)
               ggAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCCGCAGGCCgtttttcat                                                                                                                                      <  1:915040/150‑5 (MQ=255)
                 aGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGttttgttttg                                                                                                                                    <  2:1482225/150‑1 (MQ=255)
                 aGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGttttgttttg                                                                                                                                    >  1:198098/1‑150 (MQ=255)
                  gTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTtttgttttgt                                                                                                                                   >  1:1866921/1‑150 (MQ=255)
                   tGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTc                                                                                                                                  <  2:657124/150‑1 (MQ=255)
                   tGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTc                                                                                                                                  <  1:700361/150‑1 (MQ=255)
                    gggAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCCACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCt                                                                                                                                 >  1:1682231/1‑150 (MQ=255)
                    gggAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCt                                                                                                                                 >  2:1206129/1‑150 (MQ=255)
                    gggAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCt                                                                                                                                 >  2:1582160/1‑150 (MQ=255)
                    gggAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCt                                                                                                                                 >  2:1845329/1‑150 (MQ=255)
                     ggAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTATATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTcag                                                                                                                                >  2:1823784/1‑148 (MQ=255)
                           gTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGAtttt                                                                                                                          >  2:967485/1‑150 (MQ=255)
                           gTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGAtttt                                                                                                                          >  1:1168284/1‑150 (MQ=255)
                             tGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTgc                                                                                                                        <  1:255268/150‑1 (MQ=255)
                               cAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTgcgc                                                                                                                      >  2:955593/1‑150 (MQ=255)
                                 ggACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCtt                                                                                                                    >  2:2109411/1‑150 (MQ=255)
                                  gACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCACTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCttt                                                                                                                   <  2:692900/150‑1 (MQ=255)
                                    ccccGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCTCGTAAAAACTCATTTTCAACTGTCCGCGCTTTACCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCttttt                                                                                                                 <  2:285280/148‑1 (MQ=255)
                                     acagTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGTTTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTg                                                                                                                <  1:1991397/147‑1 (MQ=255)
                                          cTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAg                                                                                                           >  1:339253/1‑150 (MQ=255)
                                               ccTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAACCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTc                                                                                                      <  1:1491281/150‑1 (MQ=255)
                                                ctctCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTATGTCTGGATTTTGCGCTTTTAGCCCAGCAttac                                                                                                     >  1:684099/1‑148 (MQ=255)
                                                ctctCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTCCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCa                                                                                                     <  2:1963883/150‑1 (MQ=255)
                                                ctctCCTCCCTGGCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCa                                                                                                     <  1:2054784/150‑1 (MQ=255)
                                                 tctcCTCCCTGTCGTGGAGCAGGAAGCCTCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGGATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAg                                                                                                    <  2:98611/150‑1 (MQ=255)
                                                 tctcCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAg                                                                                                    >  1:1273034/1‑150 (MQ=255)
                                                 tctcCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTATTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAg                                                                                                    >  1:95991/1‑150 (MQ=255)
                                                   tcctccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGAc                                                                                                  <  1:1562416/150‑1 (MQ=255)
                                                    cctccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACg                                                                                                 >  2:589128/1‑150 (MQ=255)
                                                      tccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGaa                                                                                               >  2:746369/1‑150 (MQ=255)
                                                       cccTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATTAAAAACGGCCTGCGGGCCGTTTTGTTTTGTGTGGAATTTGCGCTTTTTGGCCCGCATTAAGACGaaa                                                                                              >  2:1123582/1‑150 (MQ=255)
                                                       cccTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGaaa                                                                                              >  2:1505467/1‑150 (MQ=255)
                                                       cccTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGaaa                                                                                              >  2:1808783/1‑150 (MQ=255)
                                                         cTGTCGTGGAGCAGGTAGCATGGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGTAAAACGGCCTGCGGGCGGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAAt                                                                                            <  1:1436621/150‑1 (MQ=255)
                                                            tCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGc                                                                                         >  1:636147/1‑150 (MQ=255)
                                                                ggAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCgg                                                                                     <  1:831720/150‑1 (MQ=255)
                                                                 gAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCTTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCggg                                                                                    <  2:1507062/150‑1 (MQ=255)
                                                                      ggAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTTCCCGGGAATtg                                                                               >  1:1053666/1‑150 (MQ=255)
                                                                      ggAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATtg                                                                               >  1:900615/1‑150 (MQ=255)
                                                                       taaGCATCGCGTAAAAACTGATTTGCAACTGTCGGCGCTTTTCCATTATGTTGCTAAAGATTTTATTAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATtgt                                                                              <  1:2079446/149‑1 (MQ=255)
                                                                        aaGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAATATTGCCCGGGAATtgtg                                                                             <  1:1887960/150‑1 (MQ=255)
                                                                        aaGCATCGAGTAAAAACGCATTTGCAACTTTCGGCGCTTTTCCTGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATtgtg                                                                             <  2:2091445/150‑1 (MQ=255)
                                                                         atcATCGCGTAAAAAAGCATTTGCAACTGTAGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGa                                                                            <  2:580095/148‑1 (MQ=255)
                                                                         aGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGGTGCTAAAGATTTTAAGAAAACCGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATGGTGa                                                                            <  2:199228/150‑1 (MQ=255)
                                                                           cATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTTCTAAAGATTTTATGAAAAACGGCCTGAGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGaaa                                                                          >  2:1269467/1‑150 (MQ=255)
                                                                           cATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGaaa                                                                          <  1:1615396/150‑1 (MQ=255)
                                                                            aTCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGaaaa                                                                         >  1:1898635/1‑150 (MQ=255)
                                                                              cgctTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGaaaaaa                                                                       <  1:2111118/150‑1 (MQ=255)
                                                                              cgcgTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGaaaaaa                                                                       <  1:240657/150‑1 (MQ=255)
                                                                               gcgTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAAt                                                                      >  1:1722262/1‑150 (MQ=255)
                                                                               gcgTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAAt                                                                      >  2:2200130/1‑150 (MQ=255)
                                                                               gcgTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAAt                                                                      >  1:761320/1‑150 (MQ=255)
                                                                               gcgTAAAAACGCATTTGCAAATGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTTGCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCAGGGAATTGTGAAAAAAt                                                                      >  1:1062359/1‑150 (MQ=255)
                                                                                cgTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATa                                                                     >  2:2191210/1‑150 (MQ=255)
                                                                                   aaaaaCGCATTATCAACTGGCGGCGCTTTTCCCGTTTGTTGCTAAAGAGATTATGAAAAACTGCCTGCGGGGAGTTTTGATTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATAcgc                                                                  <  2:103732/150‑1 (MQ=255)
                                                                                    aaaaCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCGTTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATAcgcg                                                                 >  1:1594129/1‑150 (MQ=255)
                                                                                    aaaaCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATAcgcg                                                                 <  2:1316887/150‑1 (MQ=255)
                                                                                         gCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCAGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAgc                                                            >  2:192870/1‑150 (MQ=255)
                                                                                            tttGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAgcgcg                                                         <  2:282302/150‑1 (MQ=255)
                                                                                              tGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCa                                                       >  1:1658901/1‑150 (MQ=255)
                                                                                              tGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCa                                                       <  1:1624962/150‑1 (MQ=255)
                                                                                                 aaCTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATa                                                    >  1:1627581/1‑150 (MQ=255)
                                                                                                  aCTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATaa                                                   >  1:2178389/1‑150 (MQ=255)
                                                                                                     gTCGGCGCTTTTCGTGTTTGTTGCTAATTATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTTTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGTAATTGTGAAAAAATACGCGACAGCGCGCAATAACCg                                                <  2:1654343/150‑1 (MQ=255)
                                                                                                      tCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGt                                               <  2:159798/150‑1 (MQ=255)
                                                                                                           ggtgttCCATTTTTTTGCTAAAGTTTTTATGATTAACGGCCTGCGGGACGTTTTGTTTTGTCTTGATTTTGCTATTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGATTTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCg                                          <  1:1372764/146‑1 (MQ=255)
                                                                                                             ttttCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGAc                                        >  2:654764/1‑150 (MQ=255)
                                                                                                                      ttgtgGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGATTTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAg                               <  2:744575/149‑1 (MQ=255)
                                                                                                                       tGTTGCTAAAGATTTTAGGAAAAACGTCTTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAATATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGt                              <  1:2001432/150‑1 (MQ=255)
                                                                                                                         ttGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGa                            >  1:1829364/1‑150 (MQ=255)
                                                                                                                          tGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCAGGGAATTGTGATAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGAt                           >  1:2214300/1‑150 (MQ=255)
                                                                                                                           gCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAATTACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATg                          <  1:837889/150‑1 (MQ=255)
                                                                                                                             tAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGcc                        >  1:1856064/1‑150 (MQ=255)
                                                                                                                               aaGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGc                      >  1:2143245/1‑150 (MQ=255)
                                                                                                                                 gATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCta                    <  1:1546102/150‑1 (MQ=255)
                                                                                                                                 gATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCta                    <  1:967485/150‑1 (MQ=255)
                                                                                                                                   ttttATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCtata                  >  2:286845/1‑150 (MQ=255)
                                                                                                                                     caaaacaaaaCGGCCCGCAGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAAt                >  2:2154060/7‑150 (MQ=255)
                                                                                                                                      tATGAAAAACGGCCTGCGGGCCGTTTAGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATAGTGAAAAAATACGCGACATCGCGCAATAACCGTTCTCGACTCATAAAAGTGATTCCGCTATAATg               >  2:1623150/1‑150 (MQ=255)
                                                                                                                                      aaTGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATg               <  1:1787508/149‑1 (MQ=255)
                                                                                                                                       aaacaaaaCGGCCCGCAGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGCCTCATAAAAGTGATGCCGCTATAATGc              >  2:1679523/5‑150 (MQ=255)
                                                                                                                                       aaacaaaaCGGCCCGCAGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACCCATAAAAGTGATGCCGCTATAATGc              >  1:43791/5‑150 (MQ=255)
                                                                                                                                       aTGAAAAACGGCCTGCGGGCATTTTTGTTTTTTATTGTTTTTGCTCTTTTTGCCCAGAATTCAGACGAAAATTGCCCGGGATTTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGc              <  2:1866921/150‑1 (MQ=255)
                                                                                                                                        tGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCAAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGcc             >  1:603035/1‑150 (MQ=255)
                                                                                                                                         acaaaaCGGCCCGCAGGCCGTTTTGTTTTGTCTGGAGTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGTAAAAATACGCGACAGCGCGCAAGACCCGTTCGCGACTCATAAATGTGATGCCGCTATATTGCcg            >  1:1712812/3‑150 (MQ=255)
                                                                                                                                            aaaCGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGt         >  1:592294/1‑150 (MQ=255)
                                                                                                                                             aaCGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCGCAGCAGTCAGACGAAATTTGCCCGGGAATTGTGAAAAAATTCGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTc        <  2:1087653/150‑1 (MQ=255)
                                                                                                                                             aaCGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTc        >  2:255744/1‑150 (MQ=255)
                                                                                                                                              aCGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCAATCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTcc       <  2:122590/150‑1 (MQ=255)
                                                                                                                                                 gCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTTTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTAt    <  1:898052/150‑1 (MQ=255)
                                                                                                                                                   cTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCCGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCAATAATTCCGCGTCCTAttt  >  2:2130267/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TACTTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTT  >  NC_000913/454705‑455001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑