Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,177,172 T→A 5.3% intergenic (+44/‑186) tufB → / → secE translation elongation factor EF‑Tu 2/preprotein translocase membrane subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,177,1720TA 5.3% 394.3 / 6.9 129intergenic (+44/‑186)tufB/secEtranslation elongation factor EF‑Tu 2/preprotein translocase membrane subunit
Reads supporting (aligned to +/- strand):  ref base T (60/61);  new base A (2/5);  total (62/67)
Fisher's exact test for biased strand distribution p-value = 4.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.44e-01

Only 100 of 132 total aligned reads displayed.

TGTTACCCTGATCCACCCGATCGCGATGGACGACGGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATACGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGT  >  NC_000913/4177023‑4177321
                                                                                                                                                     |                                                                                                                                                     
tgttACCCTGATCCACCCGATCGCGCTGGACGACGGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCAGGCGTTGTAGCAAAAGTTCTGAGCAAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCAtt                                                                                                                                                       <  1:430628/150‑1 (MQ=255)
 gttACCCTGATCCACCCGATCGCGATGGACGACGGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCAttt                                                                                                                                                      >  2:1168560/1‑150 (MQ=255)
   tACCCTGATCCACCCGATCGCGATGGACGACGGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGa                                                                                                                                                    >  2:1250613/1‑150 (MQ=255)
     cccTGATCCACCCGATCGCGATGGACGACGGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATg                                                                                                                                                  >  1:536915/1‑150 (MQ=255)
     cccTGATCCACCCGATCGCGATGGACGACGGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATg                                                                                                                                                  >  1:1394588/1‑150 (MQ=255)
     cccTGATCCACCCGATCGCGATGGACGACGGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATg                                                                                                                                                  >  1:902849/1‑150 (MQ=255)
       cTGATCCACCCGATCGCGATGGACGACGGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGcc                                                                                                                                                <  1:534980/150‑1 (MQ=255)
        tGATCCACCCGATCGCGCTGTACGACGGGCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGccc                                                                                                                                               <  2:1413156/150‑1 (MQ=255)
         gATCCACCCGATCGCGATGGACGACGGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCAAATGATGCCCt                                                                                                                                              <  2:2191243/150‑1 (MQ=255)
          aTCCACCCGATCGCGATGTACGACGGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCTCtt                                                                                                                                             >  1:896455/1‑150 (MQ=255)
           tCCACCCGATCGCGATGGACGACGGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCttt                                                                                                                                            >  2:1975805/1‑150 (MQ=255)
            ccACCCGATCGCGATGGACGACGGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCtttt                                                                                                                                           <  2:516747/150‑1 (MQ=255)
            ccACCCGATCGCGATGGACGACGGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCAAATGATGCCCtttt                                                                                                                                           <  2:545917/150‑1 (MQ=255)
            ccACCCGATCGCGATGGACGACGGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCAAATGATGCCCtttt                                                                                                                                           <  1:1048194/150‑1 (MQ=255)
             cACCCGATCGCGATGGACGACGGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCttttt                                                                                                                                          >  2:1513086/1‑150 (MQ=255)
             cACCCGATCGCGATGGACGACGGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCttttt                                                                                                                                          >  2:934194/1‑150 (MQ=255)
             cACCCGATCGCGATGGACGACGGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCAAATGATGCCCtttta                                                                                                                                          <  2:396142/150‑2 (MQ=255)
               ccccATCGCGATGGCCGCCCGGCTGCGCTTCGCAATCCGAGAAGGCGGCCGTCCCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGc                                                                                                                                        <  1:269077/150‑1 (MQ=255)
               cccGATCGCGATGGACGACGGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCAAATGATGCCCttttagt                                                                                                                                        <  1:691708/150‑4 (MQ=255)
                 cGATCGCGATGGACGACGGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCAc                                                                                                                                      >  2:2146350/1‑150 (MQ=255)
                     cgcgATGGACGACGGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCtt                                                                                                                                  >  2:1217900/1‑150 (MQ=255)
                       cgATGGACGACGGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTc                                                                                                                                >  2:862983/1‑150 (MQ=255)
                       cgATGGACGACGGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGATGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTc                                                                                                                                >  2:1088431/1‑150 (MQ=255)
                          tGGACGACGGTCTGCGTTTAGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTa                                                                                                                             <  2:584076/150‑1 (MQ=255)
                           ggACGACGGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTAc                                                                                                                            >  1:1011124/1‑150 (MQ=255)
                             acgacgGTCTGCGTTTCGCATTCCGTGAAGGCGGCCGTACCGTGGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCa                                                                                                                          <  1:198606/150‑1 (MQ=255)
                                acgGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGaa                                                                                                                       >  1:1785221/1‑150 (MQ=255)
                                 ctggatGCTTTTCGCAATCAGTGTAGGCGGCCGTACCGTTGGCGCGGGCGTTTTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAAc                                                                                                                      <  2:2053064/145‑1 (MQ=38)
                                    tCTGCGTTTCGCAATCCGTGAAGGCGGCCGGACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAACAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTg                                                                                                                   <  1:2036204/150‑1 (MQ=255)
                                       gCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCt                                                                                                                <  1:2036390/150‑1 (MQ=255)
                                         gTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAACAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCtca                                                                                                              <  1:1072285/150‑1 (MQ=255)
                                            tCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCtcatca                                                                                                           >  2:2105755/1‑150 (MQ=255)
                                             cGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAg                                                                                                          >  1:1197316/1‑150 (MQ=255)
                                              gCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGt                                                                                                         >  1:174523/1‑150 (MQ=255)
                                               cAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTg                                                                                                        <  2:1746542/150‑1 (MQ=255)
                                                  tCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGAtt                                                                                                     >  1:2070361/1‑150 (MQ=255)
                                                   ccGTGAAGTCGGCCGTACGTTTGTCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTTCTGATAACATTTGACGCAATGCGCACTAAAATGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGAttt                                                                                                    <  2:2059410/150‑1 (MQ=255)
                                                   ccGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATCTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGAttt                                                                                                    <  2:2168290/150‑1 (MQ=255)
                                                   ccGAGAAGGCGGCCGTACCGGTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGGTAATTGCCGATAACATTTGACTCAATGCTCACTAAACGGGCATCATTTGATGCCCTTTTTGCACGCTTTCTTACCAGAACCTGGCTCATCAGTGAttt                                                                                                    <  1:141260/150‑1 (MQ=255)
                                                    cGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCttttag                                                                                                                                         >  1:882651/1‑110 (MQ=255)
                                                    cGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCttttag                                                                                                                                         <  2:882651/112‑3 (MQ=255)
                                                       gAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCtt                                                                                                <  2:204150/150‑1 (MQ=255)
                                                        aaGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCttt                                                                                               <  2:2120678/150‑1 (MQ=255)
                                                         aGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCTTCAGTGATTTTCTTTg                                                                                              <  2:764607/150‑1 (MQ=255)
                                                         aGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTg                                                                                              <  2:362599/150‑1 (MQ=255)
                                                             ggcCTTACCGTTGGCGCGGGAGTTGTAGCAAAAGTTCTGAGCAAATTGCCTATAACATTTGACGCAATGAGCACTAAAATGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCAt                                                                                          <  2:76153/150‑1 (MQ=255)
                                                                     ggTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTTCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTg                                                                                  <  1:1335346/149‑1 (MQ=255)
                                                                      gTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGc                                                                                 <  2:1974045/150‑1 (MQ=255)
                                                                      gTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCAGCCTCATCAGTGATTTTCTTTGTCATAATCAATga                                                                                 >  2:1713924/1‑149 (MQ=255)
                                                                       ttGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCt                                                                                <  1:1518578/150‑1 (MQ=255)
                                                                       ttGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCt                                                                                <  1:1725117/150‑1 (MQ=255)
                                                                        tGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCtt                                                                               >  2:230157/1‑149 (MQ=255)
                                                                         ggCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTga                                                                              <  1:1020849/150‑1 (MQ=255)
                                                                             cgGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAATGGCATCATTTGATGACCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACa                                                                          <  1:130584/150‑1 (MQ=255)
                                                                                  gTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTCTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGctc                                                                     <  2:1553350/150‑1 (MQ=255)
                                                                                    tGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGCACCAGAACCTGGCGCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTg                                                                   <  1:283761/150‑1 (MQ=255)
                                                                                     gTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGt                                                                  <  2:2172559/150‑1 (MQ=255)
                                                                                      tAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGtt                                                                 <  1:338807/150‑1 (MQ=255)
                                                                                       aGCAAAAGTTCTGCGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGAACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTg                                                                <  2:313635/150‑1 (MQ=255)
                                                                                       aGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTg                                                                <  2:536915/150‑1 (MQ=255)
                                                                                       aGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTCGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATTATTGCTGAGACAGGCTCTGTTg                                                                >  2:815735/1‑150 (MQ=255)
                                                                                          aaaaGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAgg                                                             >  2:1605120/1‑150 (MQ=255)
                                                                                          aaaaGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAgg                                                             <  2:712598/150‑1 (MQ=255)
                                                                                               ttCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGta                                                        <  1:2057425/150‑1 (MQ=255)
                                                                                                tCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGtat                                                       <  1:749722/150‑1 (MQ=255)
                                                                                                       tAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTGTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTGATAATCATTGCTGAGACAGGCTCTATTGAGGGCGTATAATCCGa                                                >  2:440733/1‑150 (MQ=255)
                                                                                                        aaTTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGaa                                               >  2:1856814/1‑150 (MQ=255)
                                                                                                            gCCGATAAGATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGGTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGc                                           <  2:2036034/150‑1 (MQ=255)
                                                                                                             cctaTAACATTTGACGCAATGCGCACAAACAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCAACAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCt                                          <  1:389079/147‑1 (MQ=255)
                                                                                                             ccGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCt                                          >  1:1127404/1‑150 (MQ=255)
                                                                                                             ccGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCt                                          <  1:790523/150‑1 (MQ=255)
                                                                                                              tgATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTa                                         >  1:453989/2‑150 (MQ=255)
                                                                                                              cGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTa                                         >  2:1613674/1‑150 (MQ=255)
                                                                                                              cGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTa                                         >  2:1761387/1‑150 (MQ=255)
                                                                                                               gATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCCTTCGTACCAGAACCTGGCTCATCAGTGAATTTCTTTGTCATAATCATTGCTGAGAAAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTaa                                        >  2:1046516/1‑150 (MQ=255)
                                                                                                                        tGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATACGCGttt                               >  1:1144683/1‑150 (MQ=255)
                                                                                                                           cGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATACGCGTTTCGa                            >  2:1856999/1‑150 (MQ=255)
                                                                                                                            gCAATGCGAACTAAAAGGGCATCCTTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATACGCGTTTCTAt                           <  2:599171/150‑1 (MQ=255)
                                                                                                                             cAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATACGCGTTTCGAtt                          <  1:1713924/150‑1 (MQ=255)
                                                                                                                             cAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATACGCGTTTCGAtt                          >  1:1356255/1‑150 (MQ=255)
                                                                                                                                tGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATACGCGTTTCGATTTgg                       <  1:552868/150‑1 (MQ=255)
                                                                                                                                  cgcACTAATAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATACGCGTTTCGATTTGGtt                     <  2:902849/150‑1 (MQ=255)
                                                                                                                                  cgcACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCCGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTAGAGGGCGTATAATCCGAAAAGATAATACGCGTTTCGATTTGGtt                     >  1:51055/1‑150 (MQ=255)
                                                                                                                                  cgcACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATACGCGTTTCGATTTGGtt                     <  2:1394588/150‑1 (MQ=255)
                                                                                                                                  cgcACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATACGCGTTTCGATTTGGtt                     >  2:1966693/1‑150 (MQ=255)
                                                                                                                                  cgcACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTAGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATACGCGTTTCGATTTGGtt                     >  2:292069/1‑150 (MQ=255)
                                                                                                                                  cgcACTAAAAGGGCATCATATGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCAATGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATACGCGTTTCGATTTGGtt                     >  2:973114/1‑150 (MQ=255)
                                                                                                                                   gcACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATACGCGTTTCGATTTGGttt                    >  1:724373/1‑150 (MQ=255)
                                                                                                                                    cACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATACGCGTTTCGATTTGGTTTg                   >  2:1812132/1‑150 (MQ=255)
                                                                                                                                     gcaaaaaGGGCATCAAATGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATACGCGTTTCGATTTGGTTTGc                  >  2:1409482/4‑150 (MQ=255)
                                                                                                                                       tAAAAGGGCCTCATTTGATGCCCGTTTTGCACGCGTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCCTAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATACGCGTTTCGATTTGGTTTGCCt                <  2:1151105/150‑1 (MQ=255)
                                                                                                                                        aaaaGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATACGCGTTTCGATTTGGTTTGCCTc               >  1:2153799/1‑150 (MQ=255)
                                                                                                                                           aGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATACGCGTTTCGATTTGGTTTGCCTcgcg            <  1:297159/150‑1 (MQ=255)
                                                                                                                                           aGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATACGCGTTTCGATTTGGTTTGCCTcgcg            <  1:2069307/150‑1 (MQ=255)
                                                                                                                                             ggCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATACGCGTTTCGATTTGGTTTGCCTCGCGAt          >  2:271796/1‑150 (MQ=255)
                                                                                                                                             ggCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATACGCGTTTCGATTTGGTTTGCCTCGCGAt          >  1:1858092/1‑150 (MQ=255)
                                                                                                                                               catcatTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATACGCGTTTCGATTTGGTTTGCCTCGCGATcg        >  1:775012/1‑150 (MQ=255)
                                                                                                                                               catcatTTGATGCACTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGGGATTTTCTTTGTAATAATAAATGCTGAGACATGCTCTTTTGAGGGCGGATAATACGAAAAGCTAATACGCGTGTCGATTTGGTTTGCCTCGCGATcg        >  2:439613/1‑150 (MQ=255)
                                                                                                                                                  catTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATACGCGTTTCGATTTGGTTTGCCTCGCGATCGCgg     >  2:24088/1‑150 (MQ=255)
                                                                                                                                                     ttGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATACGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGt  <  2:627057/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGTTACCCTGATCCACCCGATCGCGATGGACGACGGTCTGCGTTTCGCAATCCGTGAAGGCGGCCGTACCGTTGGCGCGGGCGTTGTAGCAAAAGTTCTGAGCTAATTGCCGATAACATTTGACGCAATGCGCACTAAAAGGGCATCATTTGATGCCCTTTTTGCACGCTTTCGTACCAGAACCTGGCTCATCAGTGATTTTCTTTGTCATAATCATTGCTGAGACAGGCTCTGTTGAGGGCGTATAATCCGAAAAGCTAATACGCGTTTCGATTTGGTTTGCCTCGCGATCGCGGGGT  >  NC_000913/4177023‑4177321

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: