Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,231,007 G→A 5.6% L218L (CTG→CTA) ‡ rluF → 23S rRNA pseudouridine(2604) synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,231,0070GA 5.6% 369.4 / 7.5 125L218L (CTG→CTA) ‡rluF23S rRNA pseudouridine(2604) synthase
Reads supporting (aligned to +/- strand):  ref base G (61/57);  new base A (2/5);  total (63/62)
Fisher's exact test for biased strand distribution p-value = 2.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.77e-01

Only 100 of 130 total aligned reads displayed.

AAAAAAGAAGCGCCGTTTGTCTTCCGCATTACCCTGGTGCAGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTAAGATGGAAAAAACGGCGG  >  NC_000913/4230861‑4231155
                                                                                                                                                  |                                                                                                                                                    
aaaaaaGAAGCGCCGTTTGTCTTCCGCATTACCCTGGTGCAGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTgggg                                                                                                                                                   >  1:1377621/1‑150 (MQ=255)
aaaaaaGAAGCGCCGTTTGTCTTCCGCATTACCCTGGTGCAGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTgggg                                                                                                                                                   <  1:1872628/150‑1 (MQ=255)
aaaaaaGAAGCGCCGTTTGTCTTCCGCATTACCCTGGTGCAGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTgggg                                                                                                                                                   <  1:236630/150‑1 (MQ=255)
    aagaagCGCCGTTTGTCTTCCGCATTACCCTGGTGCAGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAAt                                                                                                                                               >  2:960506/1‑150 (MQ=255)
      gaagCGCCGTTTGTCTTCCGCATTACCCTGGTGCAGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATgg                                                                                                                                             <  2:394558/150‑1 (MQ=255)
       aagCGCCGTTTGTCTTCCGCATTACCCTGGTGCAGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGgc                                                                                                                                            >  1:1122475/1‑150 (MQ=255)
          cgcCGTTTGTCTTCCGCATTACCCTGGTGCAGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGcgcg                                                                                                                                         <  1:748050/150‑1 (MQ=255)
          cgcCGTTTGTCTTCCGCATTACCCTGGTGCAGGGGCTGAACCGGCAGATCCGGCGCTTGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGATCGTTAGCTTAAGCGCCATTCCGCCAGCGGAATGccgct                                                                                                                                         <  2:89718/150‑2 (MQ=255)
          cgcCGTTTGGCTTCCGCATTACCCTGGTGCAGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGCCATTCCCCCAGCGGAATGccgct                                                                                                                                         <  1:1782635/150‑2 (MQ=255)
           gcCGTTTGTCTTCCGCATTACCCTGGTGCAGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGa                                                                                                                                        >  2:689280/1‑150 (MQ=255)
           gcCGTTGGGCTTCCGCATTACCCTGGTGCAGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGCCATTCCCCCAGCGGAATGccgctt                                                                                                                                        <  2:1259462/150‑3 (MQ=255)
            ccGTTTGTCTTCCGCATTACCCTGGTGCAGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGAt                                                                                                                                       >  2:1898482/1‑150 (MQ=255)
               tttGTCTTCCGCATTACCCTGGTGCAGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTa                                                                                                                                    <  2:244430/150‑1 (MQ=255)
                  gTCTTCCGCATTACCCTGGTGCAGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAAcc                                                                                                                                 <  2:1818564/150‑1 (MQ=255)
                  gTCTTCCGCATTACCCTGGTGCAGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAAcc                                                                                                                                 <  2:854279/150‑1 (MQ=255)
                    cTTCCGCATTACCCTGGTGCAGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACcga                                                                                                                               <  2:1023215/150‑1 (MQ=255)
                      tCCGCATTACCCTGGTGCAGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGATTGGCGCGATTTAACcgacg                                                                                                                             >  2:209872/1‑150 (MQ=255)
                       ccGCATTACCCTGGTGCAGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACcgacga                                                                                                                            >  1:1118417/1‑150 (MQ=255)
                        cGCATTACGCTGGTGCCGGGGGTGAACCGCCAGATCCGGCGCTTGTGCGAGCATTTCGGCTATGAAGAGAAAAAGCTCGACCGCACGCGCTTCATGACCGTTTGCTTTAGCGGCATTCCGCTGGGGGAATGGCGCGATTAAACCGACGAt                                                                                                                           <  1:1354934/150‑1 (MQ=255)
                             tACCCTGGTGCAGGGGCTGAACGGTCAGATCCGGCGCATGTGCGAGCATTTCGTCTATGAAGTGAAAAAGCTGGAACACACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGtt                                                                                                                      <  2:927989/150‑1 (MQ=255)
                               cccTGGTGCAGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGCGAATGGCGCGATTTAACCGACGATGAGTTaa                                                                                                                    >  1:1977972/1‑150 (MQ=255)
                                 cTGGTGCAGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATc                                                                                                                  <  2:1211955/150‑1 (MQ=255)
                                  tgatGCAGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCg                                                                                                                 >  1:651900/4‑150 (MQ=255)
                                   ggTGCAGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGa                                                                                                                >  1:467680/1‑150 (MQ=255)
                                     tGCAGGTGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGAcc                                                                                                              >  2:1585136/1‑150 (MQ=255)
                                     tGCAGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGAcc                                                                                                              <  2:1507073/150‑1 (MQ=255)
                                      gCAGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACct                                                                                                             >  2:1291365/1‑150 (MQ=255)
                                      gCAGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACct                                                                                                             >  2:2131098/1‑150 (MQ=255)
                                        aGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGCGTTAATCGACctct                                                                                                           >  1:410712/1‑150 (MQ=255)
                                         ggggCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCtt                                                                                                          >  1:1583847/1‑150 (MQ=255)
                                         ggggCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCtt                                                                                                          >  1:42712/1‑150 (MQ=255)
                                           ggCTGACCCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGCGAAAAAGCGGGAACGCACGCGCATCATCAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTa                                                                                                        <  2:1496636/150‑1 (MQ=255)
                                           ggCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTa                                                                                                        <  2:837492/150‑1 (MQ=255)
                                           ggCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTa                                                                                                        <  1:1477864/150‑1 (MQ=255)
                                             cTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAg                                                                                                      >  2:380477/1‑150 (MQ=255)
                                             cTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAg                                                                                                      >  2:1611231/1‑150 (MQ=255)
                                              tGAACCGTCAGCTCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGc                                                                                                     <  1:2201125/150‑1 (MQ=255)
                                               gAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCAGCTGGGGGAATGGCGCTATTTAACCGACGATGAGTTAATCTACCTCTTTAAGCt                                                                                                    >  2:407474/1‑150 (MQ=255)
                                                  ccGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCAt                                                                                                 >  2:573084/1‑150 (MQ=255)
                                                  ccGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCAt                                                                                                 >  1:170582/1‑150 (MQ=255)
                                                   cGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATCAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCAtt                                                                                                <  1:1673856/150‑1 (MQ=255)
                                                    gTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTg                                                                                               >  2:2062551/1‑150 (MQ=255)
                                                        gATCCGGCGCATGTGCGAGGATTTCGGCTATGAAGTGAAAAAGCTGGACCGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATTAGTTAATCGACCTCTTTAAGCTCATTGaaaa                                                                                           >  1:350109/1‑150 (MQ=255)
                                                            cGGCTCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTcc                                                                                       <  1:1397742/150‑1 (MQ=255)
                                                            cGGCGCATGTGCGAGCATTTCGGCTATGAAGTGTAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTcc                                                                                       <  1:957132/150‑1 (MQ=255)
                                                            cGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTcc                                                                                       <  1:1468681/150‑1 (MQ=255)
                                                            cGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTcc                                                                                       <  2:1925709/150‑1 (MQ=255)
                                                            cGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTcc                                                                                       <  1:1162333/150‑1 (MQ=255)
                                                            cGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCGGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTcc                                                                                       <  2:37062/150‑1 (MQ=255)
                                                             ggCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTACTCGACCTCTTTAAGCTCATTGAAAATTCct                                                                                      <  1:82987/150‑1 (MQ=255)
                                                                gcATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTACGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCtt                                                                                   >  2:153065/1‑150 (MQ=255)
                                                                  aTGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTcc                                                                                 <  2:1893798/150‑1 (MQ=255)
                                                                    gtgCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGa                                                                               <  1:776016/150‑1 (MQ=255)
                                                                     tgCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAg                                                                              <  1:960506/150‑1 (MQ=255)
                                                                     ggCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGTATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAg                                                                              <  1:1780325/149‑1 (MQ=255)
                                                                       cGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGt                                                                            >  1:1141649/1‑150 (MQ=255)
                                                                              ttCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATTGCGCGATTTAACCGACGATGAGTTAATCGAACTCTTTAAGCTCATTGAAATTTCCTCTTCCGAGGTAAAACCt                                                                     <  2:256908/150‑1 (MQ=255)
                                                                               tCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTa                                                                    >  1:1495945/1‑150 (MQ=255)
                                                                                cGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTaa                                                                   <  1:1949455/150‑1 (MQ=255)
                                                                                attcTATTAAGTGAAAAAGCTGGAACGCACGCGCATCTTGAAGGTTAGCTTATGCTGCCTTCCGCTGGGGTAATGGCGCGATTTAACCTACGATTAGATAATCGACCTGTTTAAGGTCATTGAAAATTCATCTTGCGAGGTAAAACCTat                                                                   <  2:36123/147‑2 (MQ=255)
                                                                                 ggCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTACGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTaaa                                                                  >  1:1050455/1‑150 (MQ=255)
                                                                                  gCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAg                                                                 <  1:611953/150‑1 (MQ=255)
                                                                                      tGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGaa                                                             >  1:1131149/1‑150 (MQ=255)
                                                                                       gAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAg                                                            <  2:949064/150‑1 (MQ=255)
                                                                                        aaGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAgg                                                           <  2:1321368/150‑1 (MQ=255)
                                                                                          gTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGAGCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGcc                                                         <  1:1350650/150‑1 (MQ=255)
                                                                                          gTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGcc                                                         <  1:1209262/150‑1 (MQ=255)
                                                                                             aaaaaGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCaaa                                                      >  2:91459/1‑150 (MQ=255)
                                                                                              aaaaGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCaacc                                                     >  1:492053/1‑148 (MQ=255)
                                                                                              aaaaGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAAc                                                     >  1:1911673/1‑150 (MQ=255)
                                                                                               aaaGCTGGAACGCACGCGCATCATGAACGGTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTACCCGACGATGAGTTAATCGACCTCTTTAAGCGCATGGAAAATTCCTCTTCCGCGGTAAAACCTATAGCGACGGCCAAAcc                                                    >  1:326097/1‑150 (MQ=255)
                                                                                                   cTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGaaa                                                >  2:635408/1‑150 (MQ=255)
                                                                                                   cTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGAGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGaaa                                                >  2:1736349/1‑150 (MQ=255)
                                                                                                     ggAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAAc                                              <  1:1902297/150‑1 (MQ=255)
                                                                                                         cgcacgcGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCg                                          <  1:1585136/150‑1 (MQ=255)
                                                                                                             cgcgCATCATGAACGTTAGCTTAAGCTGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCa                                      <  2:285564/150‑1 (MQ=255)
                                                                                                             cgcgCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCa                                      >  2:2055979/1‑150 (MQ=255)
                                                                                                             cgcgCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCa                                      >  2:1613797/1‑150 (MQ=255)
                                                                                                                    catGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAACCGAAGGCCAAACCGAAAACAGCGGGCATCAAACg                               >  2:2193626/1‑150 (MQ=255)
                                                                                                                     atGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGt                              <  2:654696/150‑1 (MQ=255)
                                                                                                                      tGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAATATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTc                             >  1:104929/1‑150 (MQ=255)
                                                                                                                       gAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTcc                            <  1:607557/150‑1 (MQ=255)
                                                                                                                       gAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTcc                            >  2:2057307/1‑150 (MQ=255)
                                                                                                                        aaCGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCa                           >  2:504404/1‑150 (MQ=255)
                                                                                                                              aGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGtt                     <  2:446860/150‑1 (MQ=255)
                                                                                                                              aGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGtt                     <  1:573084/150‑1 (MQ=255)
                                                                                                                               gCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTa                    <  2:1286693/150‑1 (MQ=255)
                                                                                                                                cTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTaa                   <  1:1715352/150‑1 (MQ=255)
                                                                                                                                cTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAACGCCAAACCGAAAACCGCGGGCATCAAACGTCCAGTCGTTat                   >  1:367530/1‑149 (MQ=255)
                                                                                                                                  tAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTAAGa                 <  1:1278068/150‑1 (MQ=255)
                                                                                                                                    cgcgcCATTCCCCCAGCGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTAAGATg               >  2:879577/2‑150 (MQ=255)
                                                                                                                                     gcgcCATTCCCCCAGCGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTAAGATgg              >  1:1991965/1‑150 (MQ=255)
                                                                                                                                     gCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTAAGATgg              >  1:2104556/1‑150 (MQ=255)
                                                                                                                                       ggCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTAAGATGGaa            >  1:1271660/1‑150 (MQ=255)
                                                                                                                                          tttCCGCTGGGGGAATGGCGCGATTTAACGGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAATCCGAAAACAGCGGGGATCTAACGTCCAGTCGTTAAGATGGaaaaa         <  2:1977972/149‑1 (MQ=255)
                                                                                                                                           ttCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTAAGATGGaaaaaa        >  2:1839758/1‑150 (MQ=255)
                                                                                                                                                cTGGGGGAATGGCGCGATTTACCCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTAAGATGGAAAAAAcggcg   >  2:1231360/1‑150 (MQ=255)
                                                                                                                                                cTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTAAGATGGAAAAAAcggcg   >  2:807406/1‑150 (MQ=255)
                                                                                                                                                cTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTAAGATGGAAAAAAcggcg   >  2:1652271/1‑150 (MQ=255)
                                                                                                                                                 tGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGATCATTGAAAATTCCAGTTCCGAGGTAAAACCTAAAGCGAAGCACAACGCGAACAAAGCGGGCATCACACGTCCAGTCTTTATGATGTAAAAACgggcgg  >  2:1137200/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
AAAAAAGAAGCGCCGTTTGTCTTCCGCATTACCCTGGTGCAGGGGCTGAACCGTCAGATCCGGCGCATGTGCGAGCATTTCGGCTATGAAGTGAAAAAGCTGGAACGCACGCGCATCATGAACGTTAGCTTAAGCGGCATTCCGCTGGGGGAATGGCGCGATTTAACCGACGATGAGTTAATCGACCTCTTTAAGCTCATTGAAAATTCCTCTTCCGAGGTAAAACCTAAAGCGAAGGCCAAACCGAAAACAGCGGGCATCAAACGTCCAGTCGTTAAGATGGAAAAAACGGCGG  >  NC_000913/4230861‑4231155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: