Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 454,856 G→A 5.6% intergenic (+67/‑277) bolA → / → tig stationary‑phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC/peptidyl‑prolyl cis/trans isomerase (trigger factor)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913454,8560GA 5.6% 386.9 / 5.6 124intergenic (+67/‑277)bolA/tigstationary‑phase morphogene, transcriptional repressor for mreB; also regulator for dacA, dacC, and ampC/peptidyl‑prolyl cis/trans isomerase (trigger factor)
Reads supporting (aligned to +/- strand):  ref base G (59/57);  new base A (4/3);  total (63/61)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 129 total aligned reads displayed.

TTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAAT  >  NC_000913/454708‑455005
                                                                                                                                                    |                                                                                                                                                     
ttACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGCTTTTATGAAAAACGGCCTGCgg                                                                                                                                                      >  1:137728/1‑150 (MQ=255)
 tACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCggg                                                                                                                                                     >  2:1899296/1‑150 (MQ=255)
  acacTATAAAGGAGTTGGAAGGCTTGCAGGACACCGTCTTTTCCTCACCTCCCTGTCGTGGAGCATGACGCATCGCGTAAATACGCATTTTCAACTGTCGGCGCTTTTCCAGTATGTTGCTAATGATTTAATGAAAAACGGGCTGCggtc                                                                                                                                                    <  2:472634/150‑3 (MQ=255)
   cacTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGcc                                                                                                                                                   >  2:675070/1‑150 (MQ=255)
     cTATTAATGAGTGGGAAGTGATGCATGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCTTGAAGCAGCGGCTAACAACGCATTTGCAACTGTCTTCGCATTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGCCCTGCGGGCCgt                                                                                                                                                 <  2:1740660/150‑1 (MQ=255)
         tAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCgttttg                                                                                                                                             <  2:790111/150‑1 (MQ=255)
          aaGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCgttttgt                                                                                                                                            <  1:1814152/150‑1 (MQ=255)
           cggAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCCGCAGGCCgtttttca                                                                                                                                           <  1:2198575/149‑4 (MQ=255)
              aGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGttttgttttg                                                                                                                                        <  2:1482225/150‑1 (MQ=255)
              aGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGttttgttttg                                                                                                                                        >  1:198098/1‑150 (MQ=255)
               gTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTtttgttttgt                                                                                                                                       >  1:1866921/1‑150 (MQ=255)
                tGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTc                                                                                                                                      <  1:700361/150‑1 (MQ=255)
                tGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTc                                                                                                                                      <  2:657124/150‑1 (MQ=255)
                 gggAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCCACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCt                                                                                                                                     >  1:1682231/1‑150 (MQ=255)
                 gggAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCt                                                                                                                                     >  2:1206129/1‑150 (MQ=255)
                 gggAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCt                                                                                                                                     >  2:1845329/1‑150 (MQ=255)
                 gggAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCt                                                                                                                                     >  2:1582160/1‑150 (MQ=255)
                  ggAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTATATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTcag                                                                                                                                    >  2:1823784/1‑148 (MQ=255)
                        gTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGAtttt                                                                                                                              >  2:967485/1‑150 (MQ=255)
                        gTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGAtttt                                                                                                                              >  1:1168284/1‑150 (MQ=255)
                          tGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTgc                                                                                                                            <  1:255268/150‑1 (MQ=255)
                            cAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTgcgc                                                                                                                          >  2:955593/1‑150 (MQ=255)
                              ggACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCtt                                                                                                                        >  2:2109411/1‑150 (MQ=255)
                               gACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCACTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCttt                                                                                                                       <  2:692900/150‑1 (MQ=255)
                                 ccccGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCTCGTAAAAACTCATTTTCAACTGTCCGCGCTTTACCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCttttt                                                                                                                     <  2:285280/148‑1 (MQ=255)
                                  acagTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGTTTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTg                                                                                                                    <  1:1991397/147‑1 (MQ=255)
                                       cTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAg                                                                                                               >  1:339253/1‑150 (MQ=255)
                                            ccTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAACCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTc                                                                                                          <  1:1491281/150‑1 (MQ=255)
                                             ctctCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTATGTCTGGATTTTGCGCTTTTAGCCCAGCAttac                                                                                                         >  1:684099/1‑148 (MQ=255)
                                             ctctCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTCCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCa                                                                                                         <  2:1963883/150‑1 (MQ=255)
                                             ctctCCTCCCTGGCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCa                                                                                                         <  1:2054784/150‑1 (MQ=255)
                                              tctcCTCCCTGTCGTGGAGCAGGAAGCCTCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGGATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAg                                                                                                        <  2:98611/150‑1 (MQ=255)
                                              tctcCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAg                                                                                                        >  1:1273034/1‑150 (MQ=255)
                                              tctcCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTATTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAg                                                                                                        >  1:95991/1‑150 (MQ=255)
                                                tcctccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGAc                                                                                                      <  1:1562416/150‑1 (MQ=255)
                                                 cctccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACg                                                                                                     >  2:589128/1‑150 (MQ=255)
                                                   tccCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGaa                                                                                                   >  2:746369/1‑150 (MQ=255)
                                                    cccTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATTAAAAACGGCCTGCGGGCCGTTTTGTTTTGTGTGGAATTTGCGCTTTTTGGCCCGCATTAAGACGaaa                                                                                                  >  2:1123582/1‑150 (MQ=255)
                                                    cccTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGaaa                                                                                                  >  2:1808783/1‑150 (MQ=255)
                                                    cccTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGaaa                                                                                                  >  2:1505467/1‑150 (MQ=255)
                                                      cTGTCGTGGAGCAGGTAGCATGGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGTAAAACGGCCTGCGGGCGGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAAt                                                                                                <  1:1436621/150‑1 (MQ=255)
                                                         tCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGc                                                                                             >  1:636147/1‑150 (MQ=255)
                                                             ggAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCgg                                                                                         <  1:831720/150‑1 (MQ=255)
                                                              gAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCTTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCggg                                                                                        <  2:1507062/150‑1 (MQ=255)
                                                                   ggAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTTCCCGGGAATtg                                                                                   >  1:1053666/1‑150 (MQ=255)
                                                                   ggAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATtg                                                                                   >  1:900615/1‑150 (MQ=255)
                                                                    taaGCATCGCGTAAAAACTGATTTGCAACTGTCGGCGCTTTTCCATTATGTTGCTAAAGATTTTATTAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATtgt                                                                                  <  1:2079446/149‑1 (MQ=255)
                                                                     aaGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAATATTGCCCGGGAATtgtg                                                                                 <  1:1887960/150‑1 (MQ=255)
                                                                     aaGCATCGAGTAAAAACGCATTTGCAACTTTCGGCGCTTTTCCTGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATtgtg                                                                                 <  2:2091445/150‑1 (MQ=255)
                                                                      atcATCGCGTAAAAAAGCATTTGCAACTGTAGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGa                                                                                <  2:580095/148‑1 (MQ=255)
                                                                      aGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGGTGCTAAAGATTTTAAGAAAACCGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATGGTGa                                                                                <  2:199228/150‑1 (MQ=255)
                                                                        cATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTTCTAAAGATTTTATGAAAAACGGCCTGAGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGaaa                                                                              >  2:1269467/1‑150 (MQ=255)
                                                                        cATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGaaa                                                                              <  1:1615396/150‑1 (MQ=255)
                                                                         aTCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGaaaa                                                                             >  1:1898635/1‑150 (MQ=255)
                                                                           cgctTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGaaaaaa                                                                           <  1:2111118/150‑1 (MQ=255)
                                                                           cgcgTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGaaaaaa                                                                           <  1:240657/150‑1 (MQ=255)
                                                                            gcgTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAAt                                                                          >  1:761320/1‑150 (MQ=255)
                                                                            gcgTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAAt                                                                          >  2:2200130/1‑150 (MQ=255)
                                                                            gcgTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAAt                                                                          >  1:1722262/1‑150 (MQ=255)
                                                                            gcgTAAAAACGCATTTGCAAATGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTTGCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCAGGGAATTGTGAAAAAAt                                                                          >  1:1062359/1‑150 (MQ=255)
                                                                             cgTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATa                                                                         >  2:2191210/1‑150 (MQ=255)
                                                                                aaaaaCGCATTATCAACTGGCGGCGCTTTTCCCGTTTGTTGCTAAAGAGATTATGAAAAACTGCCTGCGGGGAGTTTTGATTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATAcgc                                                                      <  2:103732/150‑1 (MQ=255)
                                                                                 aaaaCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCGTTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATAcgcg                                                                     >  1:1594129/1‑150 (MQ=255)
                                                                                 aaaaCGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATAcgcg                                                                     <  2:1316887/150‑1 (MQ=255)
                                                                                      gCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCAGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAgc                                                                >  2:192870/1‑150 (MQ=255)
                                                                                         tttGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAgcgcg                                                             <  2:282302/150‑1 (MQ=255)
                                                                                           tGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCa                                                           <  1:1624962/150‑1 (MQ=255)
                                                                                           tGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCa                                                           >  1:1658901/1‑150 (MQ=255)
                                                                                              aaCTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATa                                                        >  1:1627581/1‑150 (MQ=255)
                                                                                               aCTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATaa                                                       >  1:2178389/1‑150 (MQ=255)
                                                                                                  gTCGGCGCTTTTCGTGTTTGTTGCTAATTATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTTTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGTAATTGTGAAAAAATACGCGACAGCGCGCAATAACCg                                                    <  2:1654343/150‑1 (MQ=255)
                                                                                                   tCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGt                                                   <  2:159798/150‑1 (MQ=255)
                                                                                                        ggtgttCCATTTTTTTGCTAAAGTTTTTATGATTAACGGCCTGCGGGACGTTTTGTTTTGTCTTGATTTTGCTATTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGATTTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCg                                              <  1:1372764/146‑1 (MQ=255)
                                                                                                          ttttCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGAc                                            >  2:654764/1‑150 (MQ=255)
                                                                                                                   ttgtgGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGATTTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAg                                   <  2:744575/149‑1 (MQ=255)
                                                                                                                    tGTTGCTAAAGATTTTAGGAAAAACGTCTTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAATATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGt                                  <  1:2001432/150‑1 (MQ=255)
                                                                                                                      ttGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGa                                >  1:1829364/1‑150 (MQ=255)
                                                                                                                       tGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCAGGGAATTGTGATAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGAt                               >  1:2214300/1‑150 (MQ=255)
                                                                                                                        gCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAATTACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATg                              <  1:837889/150‑1 (MQ=255)
                                                                                                                          tAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGcc                            >  1:1856064/1‑150 (MQ=255)
                                                                                                                            aaGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGc                          >  1:2143245/1‑150 (MQ=255)
                                                                                                                              gATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCta                        <  1:967485/150‑1 (MQ=255)
                                                                                                                              gATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCta                        <  1:1546102/150‑1 (MQ=255)
                                                                                                                                ttttATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCtata                      >  2:286845/1‑150 (MQ=255)
                                                                                                                                  caaaacaaaaCGGCCCGCAGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAAt                    >  2:2154060/7‑150 (MQ=255)
                                                                                                                                   tATGAAAAACGGCCTGCGGGCCGTTTAGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATAGTGAAAAAATACGCGACATCGCGCAATAACCGTTCTCGACTCATAAAAGTGATTCCGCTATAATg                   >  2:1623150/1‑150 (MQ=255)
                                                                                                                                   aaTGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATg                   <  1:1787508/149‑1 (MQ=255)
                                                                                                                                    aaacaaaaCGGCCCGCAGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGCCTCATAAAAGTGATGCCGCTATAATGc                  >  2:1679523/5‑150 (MQ=255)
                                                                                                                                    aaacaaaaCGGCCCGCAGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACCCATAAAAGTGATGCCGCTATAATGc                  >  1:43791/5‑150 (MQ=255)
                                                                                                                                    aTGAAAAACGGCCTGCGGGCATTTTTGTTTTTTATTGTTTTTGCTCTTTTTGCCCAGAATTCAGACGAAAATTGCCCGGGATTTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGc                  <  2:1866921/150‑1 (MQ=255)
                                                                                                                                     tGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCAAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGcc                 >  1:603035/1‑150 (MQ=255)
                                                                                                                                      acaaaaCGGCCCGCAGGCCGTTTTGTTTTGTCTGGAGTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGTAAAAATACGCGACAGCGCGCAAGACCCGTTCGCGACTCATAAATGTGATGCCGCTATATTGCcg                >  1:1712812/3‑150 (MQ=255)
                                                                                                                                         aaaCGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGt             >  1:592294/1‑150 (MQ=255)
                                                                                                                                          aaCGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTc            >  2:255744/1‑150 (MQ=255)
                                                                                                                                          aaCGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTc            >  2:1220391/1‑150 (MQ=255)
                                                                                                                                              gCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTTTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTAt        <  1:898052/150‑1 (MQ=255)
                                                                                                                                                cTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCCGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCAATAATTCCGCGTCCTAttt      >  2:2130267/1‑150 (MQ=255)
                                                                                                                                                cTGCGGGCCGTTTTGTTTTGTCTGGAATTTGCGGTTTTTGCCCAGCATTCAGACGAAAAATGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTAttt      <  2:137728/150‑1 (MQ=255)
                                                                                                                                                   cGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGaa   <  2:1168284/150‑1 (MQ=255)
                                                                                                                                                    gggCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAAt  >  2:1539421/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TTACACTATTAAGGAGTGGGAAGGGTTGCAGGACACCGTCTTTGCCTCTCCTCCCTGTCGTGGAGCAGGAAGCATCGCGTAAAAACGCATTTGCAACTGTCGGCGCTTTTCCAGTATGTTGCTAAAGATTTTATGAAAAACGGCCTGCGGGCCGTTTTGTTTTGTCTGGATTTTGCGCTTTTTGCCCAGCATTCAGACGAAAATTGCCCGGGAATTGTGAAAAAATACGCGACAGCGCGCAATAACCGTTCTCGACTCATAAAAGTGATGCCGCTATAATGCCGCGTCCTATTTGAAT  >  NC_000913/454708‑455005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑