Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,279,854 T→C 6.3% intergenic (+26/‑126) ghxP → / → yjcE guanine/hypoxanthine permease, high affinity; guanine/hypoxanthine:H+ symporter/putative cation/proton antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,279,8540TC 6.3% 370.5 / 11.6 126intergenic (+26/‑126)ghxP/yjcEguanine/hypoxanthine permease, high affinity; guanine/hypoxanthine:H+ symporter/putative cation/proton antiporter
Reads supporting (aligned to +/- strand):  ref base T (60/58);  new base C (3/5);  total (63/63)
Fisher's exact test for biased strand distribution p-value = 7.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.04e-01

Only 100 of 129 total aligned reads displayed.

GATCGGCTTCGCGACTCTGGTGATTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCAT  >  NC_000913/4279705‑4279999
                                                                                                                                                     |                                                                                                                                                 
gATCGGCTTCGCGACTCTGGTGATTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGt                                                                                                                                                   >  1:1237188/1‑150 (MQ=255)
gATCGGCTTCGCGACTCTGGTGATTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGt                                                                                                                                                   >  2:1171281/1‑150 (MQ=255)
 aTCGGCTTCGCGACTCTGGTGATTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTg                                                                                                                                                  <  1:1959338/150‑1 (MQ=255)
    ggCTTCGCGACTCTGGTGATTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCa                                                                                                                                               >  1:2210846/1‑150 (MQ=255)
    ggCTTCGCGACTCTGGTGATTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCa                                                                                                                                               >  2:62878/1‑150 (MQ=255)
       ttCGCGACTCTGGTGATTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATg                                                                                                                                            <  1:1684935/150‑1 (MQ=255)
       ttCGCGACTCTGGTGATTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGGGACCTTCTATGCGTGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATg                                                                                                                                            <  2:114397/150‑1 (MQ=255)
          gcgACTCTGGTGATTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCt                                                                                                                                         >  1:314303/1‑150 (MQ=255)
            gACTCTGGGGATTGGTCGTCTGGTTTCCGGCGAATGGCGAAAGTTGAACATCTGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGTGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGc                                                                                                                                       <  1:1957927/150‑1 (MQ=255)
              ctctGGTGATTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGccc                                                                                                                                     >  2:1173410/1‑150 (MQ=255)
              ctctGGTGATTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGccc                                                                                                                                     >  2:578024/1‑150 (MQ=255)
                 tGGTGATTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGtt                                                                                                                                  >  2:939925/1‑150 (MQ=255)
                  ggTGATTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGttt                                                                                                                                 >  2:2106658/1‑150 (MQ=255)
                    tGATTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGCAGCCATTGCCACCCGttttc                                                                                                                               <  1:14820/150‑2 (MQ=255)
                    tGATTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGCAGCCATTGCCACCCGttttc                                                                                                                               <  2:85863/150‑2 (MQ=255)
                    tGATTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGCAGCCATTGCCACCCGttttc                                                                                                                               <  2:2117023/150‑2 (MQ=255)
                      cttGGTCGGCTGGTATCCGGCGAATGGCGCAAGGTGAAGAGCGGTACGGTCGTTATCGGCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGCAGCCATTGCCACCCGttttcag                                                                                                                             <  1:127183/149‑4 (MQ=255)
                      aTTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTAt                                                                                                                             <  2:1482438/150‑1 (MQ=255)
                         ggTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTTGCAATGGCTGCCCGTTTTTAtttt                                                                                                                          >  2:1852695/1‑150 (MQ=255)
                            cgtcTGGTTTCCGGAGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTtctc                                                                                                                       >  1:1686805/1‑150 (MQ=255)
                             gtcTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCGTCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTcc                                                                                                                      <  2:630984/150‑1 (MQ=255)
                             gacTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTCGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCACGTTTTTATTTTATcc                                                                                                                      >  2:462757/3‑150 (MQ=255)
                               cTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGc                                                                                                                    >  1:1824394/1‑150 (MQ=255)
                               cTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTAATCGGATTCTGAAAACGGTTGGAAATGGCTGCCAGTTTTTAattttcagca                                                                                                                    >  2:214856/1‑144 (MQ=255)
                                tGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGca                                                                                                                   >  1:1608992/1‑150 (MQ=255)
                                      ccGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATtgt                                                                                                             >  2:1643836/1‑150 (MQ=255)
                                      ccGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGAGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCAAGTTTTTATTTTCTCCGCACATtgt                                                                                                             >  2:131590/1‑150 (MQ=255)
                                        ggCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTTGTGACATTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATtgtgt                                                                                                           <  2:1082040/150‑1 (MQ=255)
                                        ggCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATtgtgt                                                                                                           >  1:1434509/1‑150 (MQ=255)
                                        ggCGAATGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATtgtgtg                                                                                                          <  1:1050058/150‑1 (MQ=255)
                                         gCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTTGGCTATCTAATCTGTCATCGGAGTCTGAAAACGGGTGGCAATGGCTGCCCGTGTTTATTTTCTCCGCACATtgggtg                                                                                                          >  2:333986/1‑150 (MQ=255)
                                          cGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGa                                                                                                         <  1:1171281/150‑1 (MQ=255)
                                             aTGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCt                                                                                                      <  1:2188534/150‑1 (MQ=255)
                                                 cgcAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCACCGCACATTTTGTGAGCTTTTg                                                                                                  >  1:215699/1‑150 (MQ=255)
                                                  gcAAGTTGAACATCGGTACGGTCGTTATCGCCGGGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGc                                                                                                 <  2:219298/150‑1 (MQ=255)
                                                   cAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCg                                                                                                <  2:1119052/150‑1 (MQ=255)
                                                      gTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGttt                                                                                                                                 >  1:116586/1‑114 (MQ=255)
                                                        tGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCTATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATAtt                                                                                           >  1:51867/1‑150 (MQ=255)
                                                         gAACATCGGTTCGGTCGTTATCGCCGTGGCGCGGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTc                                                                                          <  2:1396370/150‑1 (MQ=255)
                                                         gAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTc                                                                                          <  1:100858/150‑1 (MQ=255)
                                                            cATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGa                                                                                       >  2:626966/1‑150 (MQ=255)
                                                            cATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGa                                                                                       >  1:1506978/1‑150 (MQ=255)
                                                            cATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCAATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGa                                                                                       <  2:1989416/150‑1 (MQ=255)
                                                             aTCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGaa                                                                                      >  1:1707890/1‑150 (MQ=255)
                                                             aTCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGaa                                                                                      <  2:14291/150‑1 (MQ=255)
                                                                ggTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCAGTTTTTATTTTCTCAGCACATTGTGTGAGCTTTTGCGATATTCTGaaaaa                                                                                   >  2:1319844/1‑150 (MQ=255)
                                                                   aCGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTTGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAAt                                                                                <  2:84935/150‑1 (MQ=255)
                                                                   aCGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAAt                                                                                <  2:1180372/150‑1 (MQ=255)
                                                                   aCGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAAt                                                                                <  2:1172636/150‑1 (MQ=255)
                                                                        cGTTATCGCCGTGGCGCTGGTGTACTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTTCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGaa                                                                           <  1:416900/150‑1 (MQ=255)
                                                                               gCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGc                                                                    >  1:15327/1‑150 (MQ=255)
                                                                               gCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGCGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGc                                                                    >  2:210531/1‑150 (MQ=255)
                                                                                ccGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCa                                                                   <  2:1894457/150‑1 (MQ=255)
                                                                                       gcTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCTGATTCTGATAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTc                                                            <  2:2115592/150‑1 (MQ=255)
                                                                                       gcTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTc                                                            <  2:1012145/150‑1 (MQ=255)
                                                                                       gcTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTc                                                            >  1:1770916/1‑150 (MQ=255)
                                                                                          ggTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCttt                                                         >  2:1230406/1‑150 (MQ=255)
                                                                                             gACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTtctc                                                      <  2:2201031/150‑1 (MQ=255)
                                                                                                 ttCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGAt                                                  >  1:1222187/1‑150 (MQ=255)
                                                                                                    tATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCAt                                               <  2:893465/150‑1 (MQ=255)
                                                                                                     aTGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATa                                              <  2:1682668/150‑1 (MQ=255)
                                                                                                      tGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATaa                                             <  1:1288592/150‑1 (MQ=255)
                                                                                                       gCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATaaa                                            <  1:965906/150‑1 (MQ=255)
                                                                                                       gCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAAATCAGGCATAACGTCTTTCTCAGATCATaaa                                            >  1:1342616/1‑150 (MQ=255)
                                                                                                        cGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGTCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATaaaa                                           <  1:62878/150‑1 (MQ=255)
                                                                                                         gggTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATaaaaa                                          <  1:578024/150‑1 (MQ=255)
                                                                                                          ggTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTATGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATaaaaaa                                         >  2:369279/1‑150 (MQ=255)
                                                                                                              ggtGGGCTATCTAATGTTTCATCGGATTGAGTAAACGGGTGGCTATGGCTGCCCGTTTTTATTTTCTCCGCAATTTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGTGAATTGAGGCATAACGTCTTTCTCAGATCATAAAAAACATc                                     <  2:1390590/148‑1 (MQ=255)
                                                                                                              gCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACAta                                     >  1:390395/1‑149 (MQ=255)
                                                                                                                 gggCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCa                                  >  1:1819689/1‑150 (MQ=255)
                                                                                                                   gCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCCCATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCaaa                                >  1:1186147/1‑150 (MQ=255)
                                                                                                                   gCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCaaa                                >  2:1658837/1‑150 (MQ=255)
                                                                                                                    cTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAAc                               >  1:1023948/1‑150 (MQ=255)
                                                                                                                      aTCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAg                             >  2:2025186/1‑150 (MQ=255)
                                                                                                                         tAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGa                          <  2:913494/150‑1 (MQ=255)
                                                                                                                          aaTCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGaa                         <  2:1237188/150‑1 (MQ=255)
                                                                                                                          aaTCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATCCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGaa                         >  2:532400/1‑150 (MQ=255)
                                                                                                                          aaTCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCAGAAAAAAATGAGAATTCAGGAATAACGTCTTTCTAAGATCATAAAAAACATAGCAAACAGGGaa                         >  2:1278/1‑150 (MQ=255)
                                                                                                                           aTCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAAc                        >  1:416545/1‑150 (MQ=255)
                                                                                                                             cTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGc                      >  2:105179/1‑150 (MQ=255)
                                                                                                                             cTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGc                      <  1:552916/150‑1 (MQ=255)
                                                                                                                             cTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGc                      <  1:1592683/150‑1 (MQ=255)
                                                                                                                                 cATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATgg                  >  1:2115853/1‑150 (MQ=255)
                                                                                                                                 cATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTAGTTTCTCCGCACATTTTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTATCAGATCAGAAAAAACAGCGCAAACAGTGAACGCAggg                  >  1:1501405/1‑150 (MQ=255)
                                                                                                                                 aataggATTCTGAAAACGGGTGGCAATGGCTGCCAGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATgg                  <  2:1712108/146‑1 (MQ=255)
                                                                                                                                  aTCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGa                 <  2:1770916/150‑1 (MQ=255)
                                                                                                                                     ggATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAATAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAAt              >  2:423920/1‑150 (MQ=255)
                                                                                                                                      gaaaataaaaaCGGGCAGCCATTGCCACCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAAtc             >  2:1286847/8‑150 (MQ=255)
                                                                                                                                      gATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAAtc             <  1:756395/150‑1 (MQ=255)
                                                                                                                                      gATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAAtc             <  2:552334/150‑1 (MQ=255)
                                                                                                                                       aTTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAAtct            <  1:1555195/150‑1 (MQ=255)
                                                                                                                                       aTTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAAtct            <  1:1547373/150‑1 (MQ=255)
                                                                                                                                         aataaaaaCGGGCAGCCATTGCCACCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTATCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAAtcttc          >  2:881417/5‑150 (MQ=255)
                                                                                                                                          cTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAAtcttct         <  1:939925/150‑1 (MQ=255)
                                                                                                                                           tGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGCGCTTTTGCGATATTCTGAAAAAAATTAGAATTCAGGCATTACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATcttctt        >  1:434746/1‑150 (MQ=255)
                                                                                                                                           tGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATcttctt        >  2:1953323/1‑150 (MQ=255)
                                                                                                                                            gAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCttcttc       <  1:1006940/150‑1 (MQ=255)
                                                                                                                                              aaaCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCAc     >  1:1858471/1‑150 (MQ=255)
                                                                                                                                               aaCGGGTGGCTATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCAcc    <  2:51867/150‑1 (MQ=255)
                                                                                                                                                 cGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCAt  <  1:563791/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
GATCGGCTTCGCGACTCTGGTGATTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCAT  >  NC_000913/4279705‑4279999

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: