Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,279,861 G→T 6.3% intergenic (+33/‑119) ghxP → / → yjcE guanine/hypoxanthine permease, high affinity; guanine/hypoxanthine:H+ symporter/putative cation/proton antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,279,8610GT 6.3% 374.6 / 12.3 127intergenic (+33/‑119)ghxP/yjcEguanine/hypoxanthine permease, high affinity; guanine/hypoxanthine:H+ symporter/putative cation/proton antiporter
Reads supporting (aligned to +/- strand):  ref base G (60/59);  new base T (3/5);  total (63/64)
Fisher's exact test for biased strand distribution p-value = 7.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.69e-01

Only 100 of 130 total aligned reads displayed.

TTCGCGACTCTGGTGATTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGA  >  NC_000913/4279712‑4280010
                                                                                                                                                     |                                                                                                                                                     
ttCGCGACTCTGGTGATTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATg                                                                                                                                                       <  1:1684935/150‑1 (MQ=255)
ttCGCGACTCTGGTGATTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGGGACCTTCTATGCGTGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATg                                                                                                                                                       <  2:114397/150‑1 (MQ=255)
   gcgACTCTGGTGATTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCt                                                                                                                                                    >  1:314303/1‑150 (MQ=255)
     gACTCTGGGGATTGGTCGTCTGGTTTCCGGCGAATGGCGAAAGTTGAACATCTGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGTGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGc                                                                                                                                                  <  1:1957927/150‑1 (MQ=255)
       ctctGGTGATTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGccc                                                                                                                                                >  2:1173410/1‑150 (MQ=255)
       ctctGGTGATTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGccc                                                                                                                                                >  2:578024/1‑150 (MQ=255)
          tGGTGATTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGtt                                                                                                                                             >  2:939925/1‑150 (MQ=255)
           ggTGATTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGttt                                                                                                                                            >  2:2106658/1‑150 (MQ=255)
             tGATTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGCAGCCATTGCCACCCGttttc                                                                                                                                          <  2:85863/150‑2 (MQ=255)
             tGATTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGCAGCCATTGCCACCCGttttc                                                                                                                                          <  1:14820/150‑2 (MQ=255)
               cttGGTCGGCTGGTATCCGGCGAATGGCGCAAGGTGAAGAGCGGTACGGTCGTTATCGGCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGCAGCCATTGCCACCCGttttcag                                                                                                                                        <  1:127183/149‑4 (MQ=255)
               aTTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTAt                                                                                                                                        <  2:1482438/150‑1 (MQ=255)
                  ggTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTTGCAATGGCTGCCCGTTTTTAtttt                                                                                                                                     >  2:1852695/1‑150 (MQ=255)
                     cgtcTGGTTTCCGGAGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTtctc                                                                                                                                  >  1:1686805/1‑150 (MQ=255)
                      gtcTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCGTCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTcc                                                                                                                                 <  2:630984/150‑1 (MQ=255)
                      gacTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTCGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCACGTTTTTATTTTATcc                                                                                                                                 >  2:462757/3‑150 (MQ=255)
                        cTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTAATCGGATTCTGAAAACGGTTGGAAATGGCTGCCAGTTTTTAattttcagca                                                                                                                               >  2:214856/1‑144 (MQ=255)
                               ccGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATtgt                                                                                                                        >  2:1643836/1‑150 (MQ=255)
                               ccGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGAGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCAAGTTTTTATTTTCTCCGCACATtgt                                                                                                                        >  2:131590/1‑150 (MQ=255)
                                 ggCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTTGTGACATTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATtgtgt                                                                                                                      <  2:1082040/150‑1 (MQ=255)
                                 ggCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATtgtgt                                                                                                                      >  1:1434509/1‑150 (MQ=255)
                                 ggCGAATGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATtgtgtg                                                                                                                     <  1:1050058/150‑1 (MQ=255)
                                  gCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATtgtgtg                                                                                                                     <  2:1283013/150‑1 (MQ=255)
                                   cGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGa                                                                                                                    <  1:1171281/150‑1 (MQ=255)
                                    gAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAg                                                                                                                   <  1:422287/150‑1 (MQ=255)
                                      aTGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCt                                                                                                                 <  1:2188534/150‑1 (MQ=255)
                                          cgcAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCACCGCACATTTTGTGAGCTTTTg                                                                                                             >  1:215699/1‑150 (MQ=255)
                                           gcAAGTTGAACATCGGTACGGTCGTTATCGCCGGGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGc                                                                                                            <  2:219298/150‑1 (MQ=255)
                                            cAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCg                                                                                                           <  2:1119052/150‑1 (MQ=255)
                                               gTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGttt                                                                                                                                            <  2:116586/114‑1 (MQ=255)
                                               gTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGttt                                                                                                                                            >  1:116586/1‑114 (MQ=255)
                                                 tGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCTATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATAtt                                                                                                      >  1:51867/1‑150 (MQ=255)
                                                  gAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTc                                                                                                     <  1:100858/150‑1 (MQ=255)
                                                  gAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTc                                                                                                     <  1:1490164/150‑1 (MQ=255)
                                                     cATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGa                                                                                                  >  1:1506978/1‑150 (MQ=255)
                                                     cATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGa                                                                                                  >  2:626966/1‑150 (MQ=255)
                                                     cATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCAATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGa                                                                                                  <  2:1989416/150‑1 (MQ=255)
                                                      aTCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGaa                                                                                                 <  2:14291/150‑1 (MQ=255)
                                                         ggTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCAGTTTTTATTTTCTCAGCACATTGTGTGAGCTTTTGCGATATTCTGaaaaa                                                                                              >  2:1319844/1‑150 (MQ=255)
                                                            aCGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAAt                                                                                           <  2:1180372/150‑1 (MQ=255)
                                                                 cGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGaa                                                                                      <  2:314303/150‑1 (MQ=255)
                                                                  gTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAAt                                                                                     >  1:2177585/1‑150 (MQ=255)
                                                                     aTCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGCGCTTTTGCGATATTCTGAAAAAAATGAGAATTCa                                                                                  >  2:637232/1‑150 (MQ=255)
                                                                       cGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCGGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAgg                                                                                <  1:928967/150‑1 (MQ=255)
                                                                        gCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGc                                                                               >  1:15327/1‑150 (MQ=255)
                                                                        gCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGCGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGc                                                                               >  2:210531/1‑150 (MQ=255)
                                                                         ccGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCa                                                                              <  2:1894457/150‑1 (MQ=255)
                                                                                gcTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCTGATTCTGATAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTc                                                                       <  2:2115592/150‑1 (MQ=255)
                                                                                gcTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTc                                                                       <  2:1012145/150‑1 (MQ=255)
                                                                                gcTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTc                                                                       >  1:1770916/1‑150 (MQ=255)
                                                                                   ggTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGAAATGGCTGCCCGTTTTTATTTTCTCCGAACATTGTTTGAGCTTTTGCGATATTCTGAATAAAATGAGAATTCAGGCATAACGTCttt                                                                    >  1:362957/1‑150 (MQ=255)
                                                                                      gACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTtctc                                                                 <  2:2201031/150‑1 (MQ=255)
                                                                                        ccTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAg                                                               <  1:576491/150‑1 (MQ=255)
                                                                                          ttCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGAt                                                             >  1:1222187/1‑150 (MQ=255)
                                                                                              aTGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATa                                                         <  2:1682668/150‑1 (MQ=255)
                                                                                               tGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATaa                                                        <  1:1288592/150‑1 (MQ=255)
                                                                                                gCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATaaa                                                       <  1:965906/150‑1 (MQ=255)
                                                                                                gCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAAATCAGGCATAACGTCTTTCTCAGATCATaaa                                                       >  1:1342616/1‑150 (MQ=255)
                                                                                                 cGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGTCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATaaaa                                                      <  1:62878/150‑1 (MQ=255)
                                                                                                  gggTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATaaaaa                                                     <  1:578024/150‑1 (MQ=255)
                                                                                                   ggTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTATGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATaaaaaa                                                    >  2:369279/1‑150 (MQ=255)
                                                                                                       ggtGGGCTATCTAATGTTTCATCGGATTGAGTAAACGGGTGGCTATGGCTGCCCGTTTTTATTTTCTCCGCAATTTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGTGAATTGAGGCATAACGTCTTTCTCAGATCATAAAAAACATc                                                <  2:1390590/148‑1 (MQ=255)
                                                                                                       gCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACAta                                                >  1:390395/1‑149 (MQ=255)
                                                                                                          gggCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCa                                             >  1:1819689/1‑150 (MQ=255)
                                                                                                            gCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCCCATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCaaa                                           >  1:1186147/1‑150 (MQ=255)
                                                                                                            gCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCaaa                                           >  2:1658837/1‑150 (MQ=255)
                                                                                                             cTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAAc                                          >  1:1023948/1‑150 (MQ=255)
                                                                                                               aTCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAg                                        >  2:2025186/1‑150 (MQ=255)
                                                                                                                  tAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGa                                     <  2:913494/150‑1 (MQ=255)
                                                                                                                   aaTCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGaa                                    <  2:1237188/150‑1 (MQ=255)
                                                                                                                   aaTCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATCCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGaa                                    >  2:532400/1‑150 (MQ=255)
                                                                                                                   aaTCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCAGAAAAAAATGAGAATTCAGGAATAACGTCTTTCTAAGATCATAAAAAACATAGCAAACAGGGaa                                    >  2:1278/1‑150 (MQ=255)
                                                                                                                    aTCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAAc                                   >  1:416545/1‑150 (MQ=255)
                                                                                                                      cTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGc                                 >  2:1504236/1‑150 (MQ=255)
                                                                                                                      cTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGc                                 <  1:1592683/150‑1 (MQ=255)
                                                                                                                      cTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGc                                 >  2:105179/1‑150 (MQ=255)
                                                                                                                          aataggATTCTGAAAACGGGTGGCAATGGCTGCCAGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATgg                             <  2:1712108/146‑1 (MQ=255)
                                                                                                                           aTCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGa                            <  2:1770916/150‑1 (MQ=255)
                                                                                                                           aTCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGa                            >  1:1789918/1‑150 (MQ=255)
                                                                                                                           aTCGGATTCTGAAAACGGGTGGCAATGGATGCCCATTTTTATTTTATCCGCAAATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTATTTCTCAGATCATAAAAAACATCGCAAACAGGTAACGCATGGa                            >  2:1210543/1‑150 (MQ=255)
                                                                                                                              ggATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAATAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAAt                         >  2:423920/1‑150 (MQ=255)
                                                                                                                               gATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAAtc                        <  1:756395/150‑1 (MQ=255)
                                                                                                                               gATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAAtc                        <  2:552334/150‑1 (MQ=255)
                                                                                                                                aTTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAAtct                       <  1:1547373/150‑1 (MQ=255)
                                                                                                                                aTTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAAtct                       <  1:1555195/150‑1 (MQ=255)
                                                                                                                                  aataaaaaCGGGCAGCCATTGCCACCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTATCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAAtcttc                     >  2:881417/5‑150 (MQ=255)
                                                                                                                                  aataaaaaCGGGCAGCCATTGCCACCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAAGGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAAtcttc                     >  1:1675787/5‑150 (MQ=255)
                                                                                                                                   cTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAAtcttct                    >  2:889158/1‑150 (MQ=255)
                                                                                                                                    tGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGCGCTTTTGCGATATTCTGAAAAAAATTAGAATTCAGGCATTACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATcttctt                   >  1:434746/1‑150 (MQ=255)
                                                                                                                                    tGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATcttctt                   >  2:1953323/1‑150 (MQ=255)
                                                                                                                                    tGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATcttctt                   <  2:1395401/150‑1 (MQ=255)
                                                                                                                                     gAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCttcttc                  <  1:1006940/150‑1 (MQ=255)
                                                                                                                                     gAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGAAAAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCttattc                  >  1:1860600/1‑150 (MQ=255)
                                                                                                                                       aaaCGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCAc                >  1:1858471/1‑150 (MQ=255)
                                                                                                                                        aaCGGGTGGCTATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCAcc               <  2:51867/150‑1 (MQ=255)
                                                                                                                                          cGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCAt             <  1:563791/150‑1 (MQ=255)
                                                                                                                                              gggCAATGGCTGCCCGTTTTTATTTTCTACGTACATTGTGTGAGCTTTTGAGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACtt         <  1:1582595/149‑2 (MQ=255)
                                                                                                                                               ggCAATGGCTGCCCGTTTTTATTTTCTCAGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGATTTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAAAAGGGAACGCATGGAAATCTTCTTCACCTTCCTGa        >  2:944038/1‑150 (MQ=255)
                                                                                                                                                gCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGaa       >  1:812135/1‑149 (MQ=255)
                                                                                                                                                     ggCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGa  >  1:1631245/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTCGCGACTCTGGTGATTGGTCGTCTGGTTTCCGGCGAATGGCGCAAGTTGAACATCGGTACGGTCGTTATCGCCGTGGCGCTGGTGACCTTCTATGCGGGTGGCTGGGCTATCTAATCTTTCATCGGATTCTGAAAACGGGTGGCAATGGCTGCCCGTTTTTATTTTCTCCGCACATTGTGTGAGCTTTTGCGATATTCTGAAAAAAATGAGAATTCAGGCATAACGTCTTTCTCAGATCATAAAAAACATCGCAAACAGGGAACGCATGGAAATCTTCTTCACCATACTGATAATGA  >  NC_000913/4279712‑4280010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: