Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 506,584 T→C 5.6% intergenic (+18/+19) ushA → / ← ybaK bifunctional UDP‑sugar hydrolase/5'‑nucleotidase/Cys‑tRNA(Pro)/Cys‑tRNA(Cys) deacylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913506,5840TC 5.6% 427.7 / 10.0 142intergenic (+18/+19)ushA/ybaKbifunctional UDP‑sugar hydrolase/5'‑nucleotidase/Cys‑tRNA(Pro)/Cys‑tRNA(Cys) deacylase
Reads supporting (aligned to +/- strand):  ref base T (74/59);  new base C (2/6);  total (77/65)
Fisher's exact test for biased strand distribution p-value = 1.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 143 total aligned reads displayed.

CCTTGATAACAAACCGGGCTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTTGTGCGGGGG  >  NC_000913/506437‑506730
                                                                                                                                                   |                                                                                                                                                  
 cTTGATAACAAACCGGGCTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGATAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTg                                                                                                                                                 <  1:1133677/150‑1 (MQ=255)
 cTTGATAACAAACCGGGCTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTg                                                                                                                                                 <  2:820339/150‑1 (MQ=255)
 cTTGATAACAAACCGGGCTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCGCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTg                                                                                                                                                 <  1:2171856/150‑1 (MQ=255)
    gATAACAAACCGGGCTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCAt                                                                                                                                              >  1:1960449/1‑150 (MQ=255)
      tAACAAACCGGGCTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATcc                                                                                                                                            >  2:1256613/1‑150 (MQ=255)
      tAACAAACCGGGCTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATcc                                                                                                                                            >  2:1265076/1‑150 (MQ=255)
      tAACAAACCGGGCTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATcc                                                                                                                                            >  1:716684/1‑150 (MQ=255)
      tAACAAACCGGGCTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATcc                                                                                                                                            <  1:1648436/150‑1 (MQ=255)
       aaCAAACCGGGCTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCg                                                                                                                                           >  2:1253051/1‑150 (MQ=255)
        aCAAACCGGGCTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCgg                                                                                                                                          >  1:1536917/1‑150 (MQ=255)
        aCAAACCGGGCTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACAGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCgg                                                                                                                                          >  2:216744/1‑150 (MQ=255)
        aCAAACAGGGCTATGTGAATACAGGCTTTATTGATGCCGAAGTGCTGAAAGCTTATATCCAGAAAAGCTCGCAGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGATGGAAGTAATCCGAAAGTGCCGGATGTTTGCATCCgg                                                                                                                                          >  2:1632087/1‑150 (MQ=255)
         cAAACCGGTCTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCCGGATGTGAGTGTTTATGAACAGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGCAAACATCCGGc                                                                                                                                         <  2:1503008/150‑1 (MQ=255)
         cAAACCGGGCTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGc                                                                                                                                         >  1:697125/1‑150 (MQ=255)
         cAAACCGGGCTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGCAAACATCCGGc                                                                                                                                         <  1:1897307/150‑1 (MQ=255)
         cAAACCGGGCTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGCAAACATCCGGc                                                                                                                                         <  1:1857962/150‑1 (MQ=255)
         aaaaCCGGGCTATTTTAATACCGGCTTTATTGTTGCCGAATTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGCAAACATCCGGc                                                                                                                                         <  2:935929/149‑1 (MQ=255)
                  cTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCtt                                                                                                                                >  2:546296/1‑150 (MQ=255)
                  cTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGCAAACATCCGGcactttcgga                                                                                                                                <  1:824151/150‑8 (MQ=255)
                   tATGTTATTACCGTCTTTATTGATCCCGAAGTGCTGAAAGCGTATATCCAGAAAACCTCGCCGCTGGATGAGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAAACCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTa                                                                                                                               <  1:175384/150‑1 (MQ=255)
                     tgtgAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAAt                                                                                                                             <  1:1826498/150‑1 (MQ=255)
                              cGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCggcggg                                                                                                                    >  2:1841433/1‑150 (MQ=255)
                               ggCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCggcgggc                                                                                                                   >  1:1943232/1‑150 (MQ=255)
                               ggCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCggcgggc                                                                                                                   >  1:2135787/1‑150 (MQ=255)
                                gCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAATGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCTGATGTTTGCATCCGGAACAATGATTAATCGCtgagggcg                                                                                                                  >  1:381775/1‑150 (MQ=255)
                                  tttATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGat                                                                                                                <  1:295330/150‑1 (MQ=255)
                                   ttATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGata                                                                                                               <  1:1796764/150‑1 (MQ=255)
                                     aTTGATGCCGCTGTGCTGAAAGCGTATATCCAGAAAAGCACGCCGCTGGATGGGAGTGTTAATGAAACGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTTCATCCGGCACAATGCTTAATCGCGGCTGGCGATATc                                                                                                             <  1:504660/150‑1 (MQ=255)
                                      ttGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCa                                                                                                            >  1:1736650/1‑150 (MQ=255)
                                      ttGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCa                                                                                                            >  2:1931190/1‑150 (MQ=255)
                                       tGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAg                                                                                                           >  2:368875/1‑150 (MQ=255)
                                        gaagCCGAAGTGCTGAACGCGTATATCCAGACCCGCGCGCCGCTGGATGTGACTGTTTATGACCCGAAAGGGGAGGGGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCAGCCGGCACAATGCTTCATCGCGGCGGGCGATATCAGc                                                                                                          <  1:408165/147‑1 (MQ=255)
                                        gATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGc                                                                                                          >  2:19462/1‑150 (MQ=255)
                                           gCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCaaa                                                                                                       >  1:1042205/1‑150 (MQ=255)
                                            ccGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAAt                                                                                                      >  2:2050344/1‑150 (MQ=255)
                                            ccGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAAt                                                                                                      >  2:705799/1‑150 (MQ=255)
                                             cGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAAtt                                                                                                     >  1:601214/1‑150 (MQ=255)
                                               aaGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTg                                                                                                   <  1:2061105/150‑1 (MQ=255)
                                                   gCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCTGCGGGCGATATCAGCAAATTTGGCAt                                                                                               >  2:1755389/1‑150 (MQ=255)
                                                    cTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATc                                                                                              >  1:1261608/1‑150 (MQ=255)
                                                     tGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTTATCGCGGCGGGCGATATCCGCTAATGTGGCAGCg                                                                                             >  1:356933/1‑150 (MQ=255)
                                                     tGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCg                                                                                             >  1:1832344/1‑150 (MQ=255)
                                                       aaaGCGTATATCCAGAACAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAg                                                                                           <  2:1435885/150‑1 (MQ=255)
                                                       aaaGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAg                                                                                           <  2:1185457/150‑1 (MQ=255)
                                                         aGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGa                                                                                         >  2:703549/1‑150 (MQ=255)
                                                          gCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGAt                                                                                        >  1:24225/1‑150 (MQ=255)
                                                            gTATATCCATAAAAGCTCGCCGGTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGTTGTTTGCATCCGGCTCAATGCTTAATCGCGGCTGGGGATATCAGCAAATTTGGCATCGAGGATCt                                                                                      <  2:1821024/150‑1 (MQ=255)
                                                               tatCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGTAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTg                                                                                   <  1:1437720/150‑1 (MQ=255)
                                                                atCCAGAAAAGCGCGCCGAGGGAGGGGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGc                                                                                  <  1:436851/150‑1 (MQ=255)
                                                                 tCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGcc                                                                                 <  1:952994/150‑1 (MQ=255)
                                                                  ccAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCGGGCAGTAATCCGAAAGTGCCGGCTGTTGGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCa                                                                                >  1:150917/1‑150 (MQ=255)
                                                                  ccAGAAAAGCTAGCCGCTGGATGTGAGTGTTTATGAACCGATAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCTGGCGATATGAGCAAATTTGGCATCGAGGATCTTTGCCa                                                                                <  1:2092010/150‑1 (MQ=255)
                                                                        aaGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGATGTGAGCTGGCAGTACTCCGAATGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGc                                                                          <  1:278548/150‑1 (MQ=255)
                                                                          gCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCt                                                                        <  1:368875/150‑1 (MQ=255)
                                                                              gccgcTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCGGGCAGTAATCCGAAAGTGCCGGATGGTTGCATCCGTCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTtccg                                                                    >  1:1589304/1‑150 (MQ=255)
                                                                               ccgcTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTgccgc                                                                   >  2:653009/1‑150 (MQ=255)
                                                                               ccgcTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTgccgc                                                                   >  1:1647573/1‑150 (MQ=255)
                                                                                cgcTGGATGTGAGTGTTTATGATCCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCGgccgcc                                                                  >  2:267095/1‑150 (MQ=255)
                                                                                  cTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAg                                                                <  2:2081207/150‑1 (MQ=255)
                                                                                    ggATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATAACAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGtt                                                              >  2:683800/1‑150 (MQ=255)
                                                                                          gAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGatat                                                        >  2:2042642/1‑150 (MQ=255)
                                                                                          gAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCCGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGatat                                                        >  2:2007437/1‑150 (MQ=255)
                                                                                           aGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATc                                                       <  2:1443346/150‑1 (MQ=255)
                                                                                            gtgtTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATcc                                                      <  2:1040707/150‑1 (MQ=255)
                                                                                            gtgtTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGCAAACATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATcc                                                      <  1:1080693/150‑1 (MQ=255)
                                                                                              gtgtATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGAGCTTTGCCAGATCGGCTGCCGCCAGTTCGATATCCAg                                                    <  2:1064892/147‑1 (MQ=255)
                                                                                              gtTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATACGGCACAATGCTTAATCGCGGCTGGCGATATAAGCAAATTTGGCATCGAAGATCTTTGCCAGATCGCCTGCCGCCAGTTCTATATCCat                                                    >  1:1825645/1‑149 (MQ=255)
                                                                                                 tATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTCGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTcc                                                 >  2:2027925/1‑150 (MQ=255)
                                                                                                   tGAACCGAAAGGTGAGGTGCGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCcgc                                               >  2:643077/1‑150 (MQ=255)
                                                                                                   tGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCcgc                                               >  2:1603562/1‑150 (MQ=255)
                                                                                                    gAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCcgcg                                              >  2:347960/1‑150 (MQ=255)
                                                                                                    gAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCcgcg                                              >  1:1289122/1‑150 (MQ=255)
                                                                                                        cGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTg                                          <  1:750010/150‑1 (MQ=255)
                                                                                                              gggaggggagCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGccgccg                                    <  2:1924431/148‑1 (MQ=255)
                                                                                                               tgaggtgagCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCgg                                   <  1:79993/150‑1 (MQ=255)
                                                                                                               tgaggtgagCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCgg                                   <  2:716684/150‑1 (MQ=255)
                                                                                                               tgaggtgagCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCgg                                   <  1:994623/150‑1 (MQ=255)
                                                                                                                gaggtgagCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGa                                  >  1:2086455/1‑150 (MQ=255)
                                                                                                                gaggtgagCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGa                                  <  2:2035690/150‑1 (MQ=255)
                                                                                                                 aggtgagCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGaa                                 <  1:667449/150‑1 (MQ=255)
                                                                                                                   gtgagCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAAc                               <  1:703549/150‑1 (MQ=255)
                                                                                                                        cTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATACAGTCCGCGCTTGCCGCCGGAAACATaaa                          >  1:892835/1‑150 (MQ=255)
                                                                                                                            cAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGt                      >  2:409491/1‑150 (MQ=255)
                                                                                                                             aGTAATCCGAAAGGGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTg                     <  2:77891/150‑1 (MQ=255)
                                                                                                                              gTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTgg                    <  1:13343/150‑1 (MQ=255)
                                                                                                                              gTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTgg                    >  1:205922/1‑150 (MQ=255)
                                                                                                                              gTAATACGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTgg                    <  1:216744/150‑1 (MQ=255)
                                                                                                                                   agcattgTGCCGGATGCAAACATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCAGCCGGAAACATAAATAGTGGCAAAt               >  1:227432/7‑150 (MQ=255)
                                                                                                                                     cattgTGCCGGATGCAAACATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGACGCCAGATAGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTc             >  2:388473/5‑150 (MQ=255)
                                                                                                                                       aaGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCtt           >  2:482817/1‑150 (MQ=255)
                                                                                                                                        aGTTCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTtg          <  2:723979/150‑1 (MQ=255)
                                                                                                                                         gTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTtgt         >  1:882733/1‑150 (MQ=255)
                                                                                                                                          tGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTtgtg        <  2:2086455/150‑1 (MQ=255)
                                                                                                                                          ggCCGTATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTtgtg        <  1:203019/149‑1 (MQ=255)
                                                                                                                                           gcgggATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTTGTGc       <  2:233703/147‑1 (MQ=255)
                                                                                                                                           gCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACTTAAATAGTGGCAAATTCTTGTGc       >  2:633444/1‑150 (MQ=255)
                                                                                                                                             cGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTTGTGCgg     >  2:943806/1‑150 (MQ=255)
                                                                                                                                              ggATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTTGTGCggg    <  1:1841433/150‑1 (MQ=255)
                                                                                                                                               gATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTTGTGCgggg   >  2:1524607/1‑150 (MQ=255)
                                                                                                                                                aTGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTTGTGCggggg  <  2:1960449/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CCTTGATAACAAACCGGGCTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTTGTGCGGGGG  >  NC_000913/506437‑506730

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: