Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 506,586 T→A 5.6% intergenic (+20/+17) ushA → / ← ybaK bifunctional UDP‑sugar hydrolase/5'‑nucleotidase/Cys‑tRNA(Pro)/Cys‑tRNA(Cys) deacylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913506,5860TA 5.6% 432.4 / 10.0 142intergenic (+20/+17)ushA/ybaKbifunctional UDP‑sugar hydrolase/5'‑nucleotidase/Cys‑tRNA(Pro)/Cys‑tRNA(Cys) deacylase
Reads supporting (aligned to +/- strand):  ref base T (73/59);  new base A (2/6);  total (77/65)
Fisher's exact test for biased strand distribution p-value = 1.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.12e-01

Only 100 of 145 total aligned reads displayed.

CCTTGATAACAAACCGGGCTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTTGTGCGGGGGCGTC  >  NC_000913/506437‑506734
                                                                                                                                                     |                                                                                                                                                    
ccTTGATAACAAACCGGGCTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGttt                                                                                                                                                      <  1:1854957/150‑1 (MQ=255)
 cTTGATAACAACCCGCGCTATGTTAATACCGGCTTTATTTATGCCGAAGGGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGATCCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTg                                                                                                                                                     <  2:2065304/150‑1 (MQ=255)
 cTTGATAACAAACCGGGCTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGATAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTg                                                                                                                                                     <  1:1133677/150‑1 (MQ=255)
 cTTGATAACAAACCGGGCTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTg                                                                                                                                                     <  2:820339/150‑1 (MQ=255)
 cTTGATAACAAACCGGGCTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCGCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTg                                                                                                                                                     <  1:2171856/150‑1 (MQ=255)
 cTTGAAAACAAACCGTGCTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTg                                                                                                                                                     <  1:192177/150‑1 (MQ=255)
   tGATAACAAACCGGGCTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCa                                                                                                                                                   >  2:13343/1‑150 (MQ=255)
    gATAACAAACCGGGCTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCAt                                                                                                                                                  >  1:1960449/1‑150 (MQ=255)
      tAACAAACCGGGCTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATcc                                                                                                                                                <  1:502067/150‑1 (MQ=255)
      tAACAAACCGGGCTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATcc                                                                                                                                                >  2:1256613/1‑150 (MQ=255)
      tAACAAACCGGGCTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATcc                                                                                                                                                <  1:1648436/150‑1 (MQ=255)
       aaCAAACCGGGCTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCg                                                                                                                                               >  2:1253051/1‑150 (MQ=255)
        aCAAACCGTGCTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAATCTCTCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGATGTGAGCTGGCAGTAATACGATAGTGCCGGATGTTTTCATCCgg                                                                                                                                              >  2:1561108/1‑150 (MQ=255)
        aCAAACCGGGCTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCgg                                                                                                                                              >  1:1536917/1‑150 (MQ=255)
        aCAAACCGGGCTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACAGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCgg                                                                                                                                              >  2:216744/1‑150 (MQ=255)
         cAAACCGGTCTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCCGGATGTGAGTGTTTATGAACAGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGCAAACATCCGGc                                                                                                                                             <  2:1503008/150‑1 (MQ=255)
         cAAACCGGGCTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGCAAACATCCGGc                                                                                                                                             <  1:1857962/150‑1 (MQ=255)
         aaaaCCGGGCTATTTTAATACCGGCTTTATTGTTGCCGAATTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGCAAACATCCGGc                                                                                                                                             <  2:935929/149‑1 (MQ=255)
           aaCCGGGCTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGcac                                                                                                                                           >  1:712290/1‑150 (MQ=255)
                  cTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCtt                                                                                                                                    >  2:546296/1‑150 (MQ=255)
                  cTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGCAAACATCCGGcactttcgga                                                                                                                                    <  1:824151/150‑8 (MQ=255)
                   tATGTTATTACCGTCTTTATTGATCCCGAAGTGCTGAAAGCGTATATCCAGAAAACCTCGCCGCTGGATGAGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAAACCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTa                                                                                                                                   <  1:175384/150‑1 (MQ=255)
                       tgAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATcg                                                                                                                               >  1:788576/1‑150 (MQ=255)
                             ccGGCTTTATTGATGCCGAAGTGCTGAAAGCGTTTATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGcggcgg                                                                                                                         >  2:1048767/1‑150 (MQ=255)
                             ccGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGcggcgg                                                                                                                         >  1:2081207/1‑150 (MQ=255)
                              cGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCggcggg                                                                                                                        >  2:1841433/1‑150 (MQ=255)
                               ggCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCggcgggc                                                                                                                       >  1:1943232/1‑150 (MQ=255)
                               ggCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCggcgggc                                                                                                                       >  1:2135787/1‑150 (MQ=255)
                                gCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAATGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCTGATGTTTGCATCCGGAACAATGATTAATCGCtgagggcg                                                                                                                      >  1:381775/1‑150 (MQ=255)
                                   ttATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGata                                                                                                                   <  1:1796764/150‑1 (MQ=255)
                                      ttGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCa                                                                                                                >  2:1939663/1‑150 (MQ=255)
                                      ttGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCa                                                                                                                >  1:1736650/1‑150 (MQ=255)
                                      ttGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCa                                                                                                                >  2:1931190/1‑150 (MQ=255)
                                       tGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAg                                                                                                               >  2:368875/1‑150 (MQ=255)
                                       tGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAg                                                                                                               >  1:270354/1‑150 (MQ=255)
                                        gATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGc                                                                                                              >  2:19462/1‑150 (MQ=255)
                                           gCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCaaa                                                                                                           >  1:1042205/1‑150 (MQ=255)
                                            ccGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAAt                                                                                                          >  2:2050344/1‑150 (MQ=255)
                                            ccGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAAt                                                                                                          >  2:705799/1‑150 (MQ=255)
                                             cGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAAtt                                                                                                         >  1:601214/1‑150 (MQ=255)
                                                   gCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCTGCGGGCGATATCAGCAAATTTGGCAt                                                                                                   >  2:1755389/1‑150 (MQ=255)
                                                   gCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCAt                                                                                                   >  2:1871296/1‑150 (MQ=255)
                                                   gCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCAt                                                                                                   >  2:667449/1‑150 (MQ=255)
                                                    cTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATc                                                                                                  >  1:1261608/1‑150 (MQ=255)
                                                     tGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTTATCGCGGCGGGCGATATCCGCTAATGTGGCAGCg                                                                                                 >  1:356933/1‑150 (MQ=255)
                                                     tGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGTTATCAGCAATTTTGGCATCg                                                                                                 >  1:352248/1‑150 (MQ=255)
                                                     tGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCg                                                                                                 >  1:1832344/1‑150 (MQ=255)
                                                       aaaGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAg                                                                                               <  2:1185457/150‑1 (MQ=255)
                                                         aGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGa                                                                                             >  2:703549/1‑150 (MQ=255)
                                                               tatCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGTAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTg                                                                                       <  1:1437720/150‑1 (MQ=255)
                                                                atCCAGAAAAGCGCGCCGAGGGAGGGGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGc                                                                                      <  1:436851/150‑1 (MQ=255)
                                                                 tCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGcc                                                                                     <  1:952994/150‑1 (MQ=255)
                                                                  ccAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCGGGCAGTAATCCGAAAGTGCCGGCTGTTGGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCa                                                                                    >  1:150917/1‑150 (MQ=255)
                                                                   cAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCat                                                                                   >  1:1827600/1‑149 (MQ=255)
                                                                       aaaGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCg                                                                               >  2:204122/1‑150 (MQ=255)
                                                                        aaGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGATGTGAGCTGGCAGTACTCCGAATGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGc                                                                              <  1:278548/150‑1 (MQ=255)
                                                                         aGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGcc                                                                             <  2:2023472/150‑1 (MQ=255)
                                                                          gCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTCGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCt                                                                            >  1:2034731/1‑150 (MQ=255)
                                                                               ccgcTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTgccgc                                                                       >  2:653009/1‑150 (MQ=255)
                                                                               ccgcTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTgccgc                                                                       >  1:1647573/1‑150 (MQ=255)
                                                                                cgcTGGATGTGAGTGTTTATGATCCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCGgccgcc                                                                      >  2:267095/1‑150 (MQ=255)
                                                                                cgcTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTgccgcc                                                                      <  1:390266/150‑1 (MQ=255)
                                                                                          gAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCCGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGatat                                                            >  2:2007437/1‑150 (MQ=255)
                                                                                           aGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATc                                                           <  2:1443346/150‑1 (MQ=255)
                                                                                            gtgtTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATcc                                                          <  2:1040707/150‑1 (MQ=255)
                                                                                            gtgtTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGCAAACATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATcc                                                          <  1:1080693/150‑1 (MQ=255)
                                                                                              gtgtATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGAGCTTTGCCAGATCGGCTGCCGCCAGTTCGATATCCAg                                                        <  2:1064892/147‑1 (MQ=255)
                                                                                              gtTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATACGGCACAATGCTTAATCGCGGCTGGCGATATAAGCAAATTTGGCATCGAAGATCTTTGCCAGATCGCCTGCCGCCAGTTCTATATCCat                                                        >  1:1825645/1‑149 (MQ=255)
                                                                                                 tattacacGAAAGGTGAGGTGATCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTcc                                                     <  2:381775/143‑1 (MQ=255)
                                                                                                  aTGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAAAGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCAAGTCcg                                                    >  2:1817691/1‑150 (MQ=255)
                                                                                                   tGAACCGAAAGGTGAGGTGCGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCcgc                                                   >  2:643077/1‑150 (MQ=255)
                                                                                                   tGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCcgc                                                   >  2:1603562/1‑150 (MQ=255)
                                                                                                    gAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCcgcg                                                  >  1:1289122/1‑150 (MQ=255)
                                                                                                        cGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTg                                              <  1:20946/150‑1 (MQ=255)
                                                                                                        cGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTg                                              <  1:750010/150‑1 (MQ=255)
                                                                                                               tgaggtgagCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCgg                                       <  2:1458966/150‑1 (MQ=255)
                                                                                                               tgaggtgagCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCgg                                       <  2:716684/150‑1 (MQ=255)
                                                                                                                gaggtgagCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGa                                      >  1:2086455/1‑150 (MQ=255)
                                                                                                                gaggtgagCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGa                                      <  2:2035690/150‑1 (MQ=255)
                                                                                                                 aggtgagCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGaa                                     <  1:667449/150‑1 (MQ=255)
                                                                                                                   gtgagCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAAc                                   <  1:703549/150‑1 (MQ=255)
                                                                                                                      agCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATa                                <  1:653009/150‑1 (MQ=255)
                                                                                                                        cTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATACAGTCCGCGCTTGCCGCCGGAAACATaaa                              >  1:892835/1‑150 (MQ=255)
                                                                                                                          ggCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATa                            >  1:835922/1‑150 (MQ=255)
                                                                                                                            cAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGt                          >  2:409491/1‑150 (MQ=255)
                                                                                                                             aGTAATCCGAAAGGGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTg                         <  2:77891/150‑1 (MQ=255)
                                                                                                                              gTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTgg                        <  1:1632087/150‑1 (MQ=255)
                                                                                                                              gTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTgg                        >  1:205922/1‑150 (MQ=255)
                                                                                                                              gTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTgg                        <  1:13343/150‑1 (MQ=255)
                                                                                                                                   agcattgTGCCGGATGCAAACATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCAGCCGGAAACATAAATAGTGGCAAAt                   >  1:227432/7‑150 (MQ=255)
                                                                                                                                    cGAAAGTGCCGGATGTTTGCATCAGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGACCGCGCTTGCCGCCGTAAACATTAATAGTGGCAAAtt                  >  1:224499/1‑150 (MQ=255)
                                                                                                                                       aaGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCtt               >  2:482817/1‑150 (MQ=255)
                                                                                                                                        aGTTCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTtg              <  2:723979/150‑1 (MQ=255)
                                                                                                                                          tGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTtgtg            <  2:2086455/150‑1 (MQ=255)
                                                                                                                                           gcgggATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTTGTGc           <  2:233703/147‑1 (MQ=255)
                                                                                                                                           gCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACTTAAATAGTGGCAAATTCTTGTGc           >  2:633444/1‑150 (MQ=255)
                                                                                                                                           gCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTTGTGc           <  1:975395/150‑1 (MQ=255)
                                                                                                                                             cGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTTGTGCgg         >  2:943806/1‑150 (MQ=255)
                                                                                                                                                aTGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTTGTGCggggg      <  2:271316/150‑1 (MQ=255)
                                                                                                                                                    ttGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTTGTGCGGGGGCGTc  >  2:1373274/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CCTTGATAACAAACCGGGCTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTTGTGCGGGGGCGTC  >  NC_000913/506437‑506734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: