Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 506,587 G→A 5.8% intergenic (+21/+16) ushA → / ← ybaK bifunctional UDP‑sugar hydrolase/5'‑nucleotidase/Cys‑tRNA(Pro)/Cys‑tRNA(Cys) deacylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913506,5870GA 5.8% 435.6 / 9.8 139intergenic (+21/+16)ushA/ybaKbifunctional UDP‑sugar hydrolase/5'‑nucleotidase/Cys‑tRNA(Pro)/Cys‑tRNA(Cys) deacylase
Reads supporting (aligned to +/- strand):  ref base G (76/52);  new base A (2/6);  total (79/60)
Fisher's exact test for biased strand distribution p-value = 7.24e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01

Only 100 of 144 total aligned reads displayed.

CTTGATAACAAACCGGGCTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTTGTGCGGGGGCGTC  >  NC_000913/506438‑506734
                                                                                                                                                     |                                                                                                                                                   
cTTGATAACAACCCGCGCTATGTTAATACCGGCTTTATTTATGCCGAAGGGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGATCCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTg                                                                                                                                                     <  2:2065304/150‑1 (MQ=255)
cTTGATAACAAACCGGGCTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGATAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTg                                                                                                                                                     <  1:1133677/150‑1 (MQ=255)
cTTGATAACAAACCGGGCTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTg                                                                                                                                                     <  2:820339/150‑1 (MQ=255)
cTTGATAACAAACCGGGCTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCGCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTg                                                                                                                                                     <  1:2171856/150‑1 (MQ=255)
cTTGAAAACAAACCGTGCTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTg                                                                                                                                                     <  1:192177/150‑1 (MQ=255)
  tGATAACAAACCGGGCTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCa                                                                                                                                                   >  2:13343/1‑150 (MQ=255)
   gATAACAAACCGGGCTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCAt                                                                                                                                                  >  1:1960449/1‑150 (MQ=255)
     tAACAAACCGGGCTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATcc                                                                                                                                                >  1:716684/1‑150 (MQ=255)
     tAACAAACCGGGCTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATcc                                                                                                                                                >  2:1265076/1‑150 (MQ=255)
     tAACAAACCGGGCTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATcc                                                                                                                                                <  1:1648436/150‑1 (MQ=255)
      aaCAAACCGGGCTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCg                                                                                                                                               >  2:1253051/1‑150 (MQ=255)
       aCAAACCGTGCTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAATCTCTCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGATGTGAGCTGGCAGTAATACGATAGTGCCGGATGTTTTCATCCgg                                                                                                                                              >  2:1561108/1‑150 (MQ=255)
       aCAAACCGGGCTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCgg                                                                                                                                              >  1:1536917/1‑150 (MQ=255)
       aCAAACCGGGCTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACAGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCgg                                                                                                                                              >  2:216744/1‑150 (MQ=255)
       aCAAACAGGGCTATGTGAATACAGGCTTTATTGATGCCGAAGTGCTGAAAGCTTATATCCAGAAAAGCTCGCAGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGATGGAAGTAATCCGAAAGTGCCGGATGTTTGCATCCgg                                                                                                                                              >  2:1632087/1‑150 (MQ=255)
        cAAACCGGGCTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGc                                                                                                                                             >  1:697125/1‑150 (MQ=255)
        cAAACCGGGCTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGCAAACATCCGGc                                                                                                                                             <  1:1897307/150‑1 (MQ=255)
        aaaaCCGGGCTATTTTAATACCGGCTTTATTGTTGCCGAATTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGCAAACATCCGGc                                                                                                                                             <  2:935929/149‑1 (MQ=255)
                 cTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCtt                                                                                                                                    >  2:546296/1‑150 (MQ=255)
                 cTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGCAAACATCCGGcactttcgga                                                                                                                                    <  1:824151/150‑8 (MQ=255)
                  tATGTTATTACCGTCTTTATTGATCCCGAAGTGCTGAAAGCGTATATCCAGAAAACCTCGCCGCTGGATGAGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAAACCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTa                                                                                                                                   <  1:175384/150‑1 (MQ=255)
                             cGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCggcggg                                                                                                                        >  2:1841433/1‑150 (MQ=255)
                               gCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAATGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCTGATGTTTGCATCCGGAACAATGATTAATCGCtgagggcg                                                                                                                      >  1:381775/1‑150 (MQ=255)
                               gCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCggcgggcg                                                                                                                      <  2:1188865/150‑1 (MQ=255)
                                 tttATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGat                                                                                                                    <  1:295330/150‑1 (MQ=255)
                                  ttATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGata                                                                                                                   <  1:1796764/150‑1 (MQ=255)
                                    aTTGATGCCGCTGTGCTGAAAGCGTATATCCAGAAAAGCACGCCGCTGGATGGGAGTGTTAATGAAACGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTTCATCCGGCACAATGCTTAATCGCGGCTGGCGATATc                                                                                                                 <  1:504660/150‑1 (MQ=255)
                                     ttGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCa                                                                                                                >  1:1736650/1‑150 (MQ=255)
                                     ttGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCa                                                                                                                >  2:1931190/1‑150 (MQ=255)
                                     ttGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCa                                                                                                                >  2:1939663/1‑150 (MQ=255)
                                      tGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAg                                                                                                               >  2:368875/1‑150 (MQ=255)
                                       gaagCCGAAGTGCTGAACGCGTATATCCAGACCCGCGCGCCGCTGGATGTGACTGTTTATGACCCGAAAGGGGAGGGGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCAGCCGGCACAATGCTTCATCGCGGCGGGCGATATCAGc                                                                                                              <  1:408165/147‑1 (MQ=255)
                                          gCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCaaa                                                                                                           >  1:1042205/1‑150 (MQ=255)
                                           ccGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAAt                                                                                                          >  2:2050344/1‑150 (MQ=255)
                                           ccGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAAt                                                                                                          >  2:705799/1‑150 (MQ=255)
                                            cGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAAtt                                                                                                         >  1:601214/1‑150 (MQ=255)
                                              aaGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTg                                                                                                       <  1:2061105/150‑1 (MQ=255)
                                                  gCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCTGCGGGCGATATCAGCAAATTTGGCAt                                                                                                   >  2:1755389/1‑150 (MQ=255)
                                                  gCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCAt                                                                                                   >  2:1871296/1‑150 (MQ=255)
                                                   cTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATc                                                                                                  >  1:1261608/1‑150 (MQ=255)
                                                    tGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGTTATCAGCAATTTTGGCATCg                                                                                                 >  1:352248/1‑150 (MQ=255)
                                                    tGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCg                                                                                                 >  1:1832344/1‑150 (MQ=255)
                                                        aGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGa                                                                                             >  2:703549/1‑150 (MQ=255)
                                                         gCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGAt                                                                                            >  1:24225/1‑150 (MQ=255)
                                                           gTATATCCATAAAAGCTCGCCGGTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGTTGTTTGCATCCGGCTCAATGCTTAATCGCGGCTGGGGATATCAGCAAATTTGGCATCGAGGATCt                                                                                          <  2:1821024/150‑1 (MQ=255)
                                                              tatCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGTAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTg                                                                                       <  1:1437720/150‑1 (MQ=255)
                                                               atCCAGAAAAGCGCGCCGAGGGAGGGGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGc                                                                                      <  1:436851/150‑1 (MQ=255)
                                                                 ccAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCGGGCAGTAATCCGAAAGTGCCGGCTGTTGGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCa                                                                                    >  1:150917/1‑150 (MQ=255)
                                                                 ccAGAAAAGCTAGCCGCTGGATGTGAGTGTTTATGAACCGATAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCTGGCGATATGAGCAAATTTGGCATCGAGGATCTTTGCCa                                                                                    <  1:2092010/150‑1 (MQ=255)
                                                                  cAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCat                                                                                   >  1:1827600/1‑149 (MQ=255)
                                                                       aaGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGATGTGAGCTGGCAGTACTCCGAATGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGc                                                                              <  1:278548/150‑1 (MQ=255)
                                                                         gCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCt                                                                            <  1:368875/150‑1 (MQ=255)
                                                                             gccgcTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCGGGCAGTAATCCGAAAGTGCCGGATGGTTGCATCCGTCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTtccg                                                                        >  1:1589304/1‑150 (MQ=255)
                                                                              ccgcTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTgccgc                                                                       >  1:1647573/1‑150 (MQ=255)
                                                                              ccgcTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTgccgc                                                                       >  2:653009/1‑150 (MQ=255)
                                                                               cgcTGGATGTGAGTGTTTATGATCCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCGgccgcc                                                                      >  2:267095/1‑150 (MQ=255)
                                                                                   ggATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATAACAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGtt                                                                  >  2:683800/1‑150 (MQ=255)
                                                                                         gAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGatat                                                            >  2:2042642/1‑150 (MQ=255)
                                                                                         gAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCCGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGatat                                                            >  2:2007437/1‑150 (MQ=255)
                                                                                          aGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATc                                                           <  2:1443346/150‑1 (MQ=255)
                                                                                           gtgtTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATcc                                                          <  2:1040707/150‑1 (MQ=255)
                                                                                           gtgtTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGCAAACATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATcc                                                          <  1:1080693/150‑1 (MQ=255)
                                                                                             gtgtATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGAGCTTTGCCAGATCGGCTGCCGCCAGTTCGATATCCAg                                                        <  2:1064892/147‑1 (MQ=255)
                                                                                             gtTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATACGGCACAATGCTTAATCGCGGCTGGCGATATAAGCAAATTTGGCATCGAAGATCTTTGCCAGATCGCCTGCCGCCAGTTCTATATCCat                                                        >  1:1825645/1‑149 (MQ=255)
                                                                                                tattacacGAAAGGTGAGGTGATCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTcc                                                     <  2:381775/143‑1 (MQ=255)
                                                                                                tATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTCGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTcc                                                     >  2:2027925/1‑150 (MQ=255)
                                                                                                  tGAACCGAAAGGTGAGGTGCGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCcgc                                                   >  2:643077/1‑150 (MQ=255)
                                                                                                   gAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCcgcg                                                  >  2:347960/1‑150 (MQ=255)
                                                                                                   gAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCcgcg                                                  >  1:1289122/1‑150 (MQ=255)
                                                                                                       cGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTg                                              <  1:750010/150‑1 (MQ=255)
                                                                                                       cGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTg                                              <  1:20946/150‑1 (MQ=255)
                                                                                                              tgaggtgagCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCgg                                       <  2:1458966/150‑1 (MQ=255)
                                                                                                              tgaggtgagCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCgg                                       <  1:79993/150‑1 (MQ=255)
                                                                                                              tgaggtgagCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCgg                                       <  2:716684/150‑1 (MQ=255)
                                                                                                              tgaggtgagCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCgg                                       <  1:994623/150‑1 (MQ=255)
                                                                                                               gaggtgagCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGa                                      <  2:2035690/150‑1 (MQ=255)
                                                                                                               gaggtgagCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGa                                      >  1:2086455/1‑150 (MQ=255)
                                                                                                                aggtgagCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGaa                                     <  1:667449/150‑1 (MQ=255)
                                                                                                                  gtgagCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAAc                                   <  1:703549/150‑1 (MQ=255)
                                                                                                                       cTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATACAGTCCGCGCTTGCCGCCGGAAACATaaa                              >  1:892835/1‑150 (MQ=255)
                                                                                                                           cAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGt                          >  2:409491/1‑150 (MQ=255)
                                                                                                                            aGTAATCCGAAAGGGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTg                         <  2:77891/150‑1 (MQ=255)
                                                                                                                             gTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTgg                        <  1:13343/150‑1 (MQ=255)
                                                                                                                             gTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTgg                        >  1:205922/1‑150 (MQ=255)
                                                                                                                             gTAATACGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTgg                        <  1:216744/150‑1 (MQ=255)
                                                                                                                                  agcattgTGCCGGATGCAAACATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCAGCCGGAAACATAAATAGTGGCAAAt                   >  1:227432/7‑150 (MQ=255)
                                                                                                                                      aaGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCtt               >  2:482817/1‑150 (MQ=255)
                                                                                                                                       aGTTCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTtg              <  2:723979/150‑1 (MQ=255)
                                                                                                                                        gTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTtgt             >  1:882733/1‑150 (MQ=255)
                                                                                                                                         tGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTtgtg            <  2:2086455/150‑1 (MQ=255)
                                                                                                                                         ggCCGTATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTtgtg            <  1:203019/149‑1 (MQ=255)
                                                                                                                                          gcgggATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTTGTGc           <  2:233703/147‑1 (MQ=255)
                                                                                                                                          gCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACTTAAATAGTGGCAAATTCTTGTGc           >  2:633444/1‑150 (MQ=255)
                                                                                                                                          gCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTTGTGc           <  1:975395/150‑1 (MQ=255)
                                                                                                                                            cGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTTGTGCgg         >  2:943806/1‑150 (MQ=255)
                                                                                                                                             ggATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTTGTGCggg        <  1:1841433/150‑1 (MQ=255)
                                                                                                                                              gATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTTGTGCgggg       >  2:1524607/1‑150 (MQ=255)
                                                                                                                                               aTGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTTGTGCggggg      <  2:1960449/150‑1 (MQ=255)
                                                                                                                                                   ttGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTTGTGCGGGGGCGTc  >  2:1373274/1‑150 (MQ=255)
                                                                                                                                                   ttGCATACGGAACAATGATTAATCGCGGCGGGCGATATCAGAAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACTTAAATAGTGGCAAATTCTTGTGCGGGGGCGTc  >  2:1061446/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
CTTGATAACAAACCGGGCTATGTGAATACCGGCTTTATTGATGCCGAAGTGCTGAAAGCGTATATCCAGAAAAGCTCGCCGCTGGATGTGAGTGTTTATGAACCGAAAGGTGAGGTGAGCTGGCAGTAATCCGAAAGTGCCGGATGTTTGCATCCGGCACAATGCTTAATCGCGGCGGGCGATATCAGCAAATTTGGCATCGAGGATCTTTGCCAGATCGCCTGCCGCCAGTTCGATATCCAGTCCGCGCTTGCCGCCGGAAACATAAATAGTGGCAAATTCTTGTGCGGGGGCGTC  >  NC_000913/506438‑506734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: