Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 696,782 G→C 11.4% noncoding (49/75 nt) glnW ← tRNA‑Gln

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913696,7820GC11.4% 100.8 / 6.4 35noncoding (49/75 nt)glnWtRNA‑Gln
Reads supporting (aligned to +/- strand):  ref base G (23/8);  new base C (2/2);  total (25/10)
Fisher's exact test for biased strand distribution p-value = 5.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

CCGAACATATTCGATACATTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGC  >  NC_000913/696637‑696925
                                                                                                                                                 |                                                                                                                                               
ccGAACATATTCGCTACATTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGaa                                                                                                                                             <  1:1986132/150‑1 (MQ=255)
    aCATATTCGATACATTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGcc                                                                                                                                         >  1:1749916/1‑150 (MQ=255)
     cATATTCGATACATTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCg                                                                                                                                        >  1:1672049/1‑150 (MQ=255)
     cATATTCGATACATTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCg                                                                                                                                        <  1:1985335/150‑1 (MQ=255)
      atatTCGATACATTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATTAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCgg                                                                                                                                       <  1:1983631/150‑1 (MQ=255)
       tatTCGATACATTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGt                                                                                                                                      >  2:1895271/1‑150 (MQ=255)
           cGATACATTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCa                                                                                                                                  >  2:726943/1‑150 (MQ=255)
            gATACATTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCaa                                                                                                                                 >  1:1200553/1‑150 (MQ=255)
              tACATTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCaaaa                                                                                                                               >  1:1679744/1‑150 (MQ=255)
                 aTTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAAcc                                                                                                                            >  2:226433/1‑150 (MQ=255)
                    cAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGt                                                                                                                         >  1:1823370/1‑150 (MQ=255)
                              tggCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGc                                                                                                               >  2:824595/1‑150 (MQ=255)
                                  tACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTgg                                                                                                           >  1:1691382/1‑150 (MQ=255)
                                    cgacgGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCg                                                                                                         >  1:2009713/1‑150 (MQ=255)
                                    cgacgGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCg                                                                                                         >  2:1006613/1‑150 (MQ=255)
                                      acgGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGAt                                                                                                       >  1:1221366/1‑150 (MQ=255)
                                      acgGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGAt                                                                                                       <  2:1988861/150‑1 (MQ=255)
                                           aTTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATAcccc                                                                                                  >  2:1157452/1‑150 (MQ=255)
                                               gAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACAAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGAGATACCCCaaaa                                                                                              >  1:123891/1‑148 (MQ=255)
                                                  ccTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGTATTCGAACCAGGGAATGCCGGTATC‑AAAACCCGTGCCATAACCCCTGGCCAAAACCCAAtcagcc                                                                                          >  2:778162/1‑145 (MQ=25)
                                                        aCCCCTTCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCgg                                                                                     <  1:1702654/150‑1 (MQ=255)
                                                                       gTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCg                                                                      >  1:1930084/1‑150 (MQ=255)
                                                                              gCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAAAAACCGGGCGGTGACCCGCTTACTCGAagaaga                                                               >  1:11913/1‑150 (MQ=255)
                                                                                    aCCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATAAGACGGGCGGTGAAACGTTTAGTCGACGATGCTCGCTg                                                         >  1:679874/1‑150 (MQ=18)
                                                                                     ccAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAAAAAACGAGCGGTGAAACGCTTAATCGAAGAAGATCGATgg                                                        >  1:612558/1‑150 (MQ=21)
                                                                                           gAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATACACGGCCGGTTAACCGATAACTCGTAGAAGATGGATGGGGTAcc                                                  >  1:1646118/1‑150 (MQ=21)
                                                                                            aGCTACGTAGCCGGATTGTTTCTTCGATGGCTGGGTTACCTTGATGCGATCCAGGGATTGCCGGTATCAAAACCCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGTGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCt                                                 <  2:1288963/150‑1 (MQ=25)
                                                                                             gCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTg                                                <  2:1930084/150‑1 (MQ=255)
                                                                                                 cGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAAGAGACGGGCGGTGACCCGAGTAGTCGAAGATGATGGCTGGGGTACCtcgctg                                            >  1:955801/1‑145 (MQ=18)
                                                                                                                           tGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCaaaa                  <  2:1691382/150‑1 (MQ=255)
                                                                                                                                    aggATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCgaag                                                                   >  2:281735/2‑92 (MQ=14)
                                                                                                                                    aggATTCGAACCTCGGAATGCCGGAATCAGAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCgaag                                                                   <  1:281735/91‑1 (MQ=17)
                                                                                                                                     ggATTCGAACCTCGGAATGCCGGAATCATAATCCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCgaagaa                                                                 <  1:774539/93‑1 (MQ=17)
                                                                                                                                     ggATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCgaagaa                                                                 >  2:774539/1‑93 (MQ=17)
                                                                                                                                           gAACCAGGGAATGCCGGTATCAAAAACCGGTACCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGc  >  1:1693612/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
CCGAACATATTCGATACATTCAGAATTTGGTGGCTACGACGGGATTCGAACCTGTGACCCCATCATTATGAGTGATGTGCTCTAACCAACTGAGCTACGTAGCCAGATTGTTTCTTCGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAATAACCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGC  >  NC_000913/696637‑696925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑