Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 10,622 A→C 6.7% intergenic (‑128/+21) satP ← / ← yaaW succinate‑acetate transporter/UPF0174 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091310,6220AC 6.7% 370.8 / 13.0 120intergenic (‑128/+21)satP/yaaWsuccinate‑acetate transporter/UPF0174 family protein
Reads supporting (aligned to +/- strand):  ref base A (54/58);  new base C (2/6);  total (56/64)
Fisher's exact test for biased strand distribution p-value = 2.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01

Only 100 of 136 total aligned reads displayed.

TAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGCCGCACCTACAGGGCCTCCC  >  NC_000913/10473‑10769
                                                                                                                                                     |                                                                                                                                                   
tAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGgaaaatgaaa                                                                                                                                                     <  1:2153099/150‑1 (MQ=255)
 aGCCAAGTTAGTGTTGCCCATAATTCCTCAAAAAACATCATCGAATGCATGGTGAAATAATTTCCCTGAATAACCGTAGTGTTTTCAGGGCGCGGCATACTTATCAGCCAAGGGGGCAGTGTCTACGATCTTTTCAGGGgaaaatgaaaa                                                                                                                                                    >  2:568381/1‑150 (MQ=255)
 aGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGCGGGgaaaatgaaaa                                                                                                                                                    >  2:1472527/1‑150 (MQ=255)
  gCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACTATCTTTTGAGGGgaaaatgaaaat                                                                                                                                                   >  2:54463/1‑150 (MQ=255)
  gCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGgaaaatgaaaat                                                                                                                                                   >  1:211582/1‑150 (MQ=255)
     aaCTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAAtttt                                                                                                                                                <  2:1274801/150‑1 (MQ=255)
      aCTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGAGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATATTc                                                                                                                                               >  1:1061535/1‑150 (MQ=255)
       cTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTcc                                                                                                                                              <  1:454642/150‑1 (MQ=255)
        ttAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTccc                                                                                                                                             <  1:1008012/150‑1 (MQ=255)
         tAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCTGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTcccc                                                                                                                                            >  2:1790530/1‑150 (MQ=255)
         tAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATTTTCATTTTcccc                                                                                                                                            <  2:860415/150‑1 (MQ=255)
         tAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATTTTCATTTTcccc                                                                                                                                            <  1:351757/150‑1 (MQ=255)
         tAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATTTTCATTTTcccc                                                                                                                                            <  2:782423/150‑1 (MQ=255)
         tAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATTTTCATTTTcccc                                                                                                                                            <  1:1791923/150‑1 (MQ=255)
            tgtTTCCCATAATTACTCAAAAATCATCATCGAATGAATGGTGAAATAATTTAACTGAATAACTGTAGTGTTTTCAGGGCGCGGCAAAAAAATCAGCAAGTCGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCACGGt                                                                                                                                         >  2:1338770/1‑150 (MQ=255)
             gtTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGtt                                                                                                                                        <  2:641809/150‑1 (MQ=255)
               tGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTc                                                                                                                                      >  1:299598/1‑150 (MQ=255)
                  ccATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCATCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCgg                                                                                                                                   <  1:356884/150‑1 (MQ=255)
                  ccATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCgg                                                                                                                                   >  2:1788674/1‑150 (MQ=255)
                   cATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGt                                                                                                                                  >  2:799281/1‑150 (MQ=255)
                   cATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCACCGGTTTCCGGt                                                                                                                                  >  1:910684/1‑150 (MQ=255)
                    aTAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTa                                                                                                                                 <  1:1734393/150‑1 (MQ=255)
                      aaTTACTCAAAAATCATCATCGAATGAATTGTGAAATAATTTCCCTGAATAACTGAAGTGTTTTCAGGGCGCGGCATATTAATCAGAGAGTGGTGCAGTTTCGACGATCTTTTGAGGGGAATATGAAATTTTTCCCCGTGTTCCGGTAta                                                                                                                               >  2:135991/1‑149 (MQ=255)
                             cAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTg                                                                                                                        <  2:1665574/150‑1 (MQ=255)
                             cAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTg                                                                                                                        >  2:1380293/1‑150 (MQ=255)
                                 aaTCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATTATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTg                                                                                                                    <  2:1848650/150‑1 (MQ=255)
                                  atcatcatcGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTgg                                                                                                                   <  2:2214504/150‑1 (MQ=255)
                                  atcatcatcGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTgg                                                                                                                   <  1:1842495/150‑1 (MQ=255)
                                  atcatcatcGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTgg                                                                                                                   <  2:1507541/150‑1 (MQ=255)
                                     atcatcGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGgcgc                                                                                                                <  2:855498/150‑1 (MQ=255)
                                     atcatcGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGgcgc                                                                                                                <  2:610153/150‑1 (MQ=255)
                                       catcGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTa                                                                                                              >  1:1142893/1‑150 (MQ=255)
                                       catcGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGCTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTa                                                                                                              <  1:1568514/150‑1 (MQ=255)
                                         tcGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAAc                                                                                                            <  2:11023/150‑1 (MQ=255)
                                           gaatgaatGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCa                                                                                                          >  1:1308805/1‑150 (MQ=255)
                                              tgaatgGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATcc                                                                                                       >  1:539438/1‑150 (MQ=255)
                                               taatgGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGTGGCAGTGTCTTCGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGAGCTGAGTGGCGCTAACCATCCg                                                                                                      <  2:213153/149‑1 (MQ=255)
                                                 atgGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGgc                                                                                                    <  1:812078/150‑1 (MQ=255)
                                                  tgGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGgcg                                                                                                   >  2:1978505/1‑150 (MQ=255)
                                                   ggTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGgcgc                                                                                                  >  2:2175195/1‑150 (MQ=255)
                                                    gTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGTCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCgca                                                                                                 >  1:1141500/1‑150 (MQ=255)
                                                    gTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCgca                                                                                                 <  1:1819726/150‑1 (MQ=255)
                                                                 ccTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTg                                                                                    <  1:525858/150‑1 (MQ=255)
                                                                 ccTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTg                                                                                    >  1:2089071/1‑150 (MQ=255)
                                                                 ccTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCACCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGTCGCAGGCAGGCGAtttt                                                                                    >  1:879592/1‑149 (MQ=255)
                                                                  cTGAATAACTGTAGTGTTTTCAGGGAGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGc                                                                                   >  2:369205/1‑150 (MQ=255)
                                                                  cTGAATAACTGTAGGGTTTGCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGc                                                                                   <  1:314984/150‑1 (MQ=255)
                                                                     aaTAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCTGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGt                                                                                <  1:1790530/150‑1 (MQ=255)
                                                                        aaCTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACg                                                                             >  1:281373/1‑150 (MQ=255)
                                                                          cTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTCCGGc                                                                           >  2:1093461/1‑150 (MQ=255)
                                                                          cTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGc                                                                           <  2:876400/150‑1 (MQ=255)
                                                                            gTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTg                                                                         <  2:1924974/150‑1 (MQ=255)
                                                                            gTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGGGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTg                                                                         <  1:843866/150‑1 (MQ=255)
                                                                              aGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGCGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTACCCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGa                                                                       >  1:1712592/1‑150 (MQ=255)
                                                                               gtgtTTTCAGGGCGCGGCATCATCATCATCCAGTGGGTCAGGGGCTACGAGCTTTTGAGTGGAAACTGAAAATTTTCCCCGGTTTCAGGTATCAGACCTGAGGTTCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGaa                                                                      <  1:630679/150‑1 (MQ=255)
                                                                                tgtTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAAt                                                                     >  1:1227617/1‑150 (MQ=255)
                                                                                 gtTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCTGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATc                                                                    >  2:909416/1‑150 (MQ=255)
                                                                                   tttCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGt                                                                  >  2:1779740/1‑150 (MQ=255)
                                                                                     tCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCa                                                                >  1:1733759/1‑150 (MQ=255)
                                                                                        gggCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCAcgc                                                             <  2:129390/150‑1 (MQ=255)
                                                                                            gcgGCATAATAATCAGCCCGGGCGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATa                                                         <  1:102599/150‑1 (MQ=255)
                                                                                               gCAAAATAATCAGCCAGTGGGGCAGTGTCTACCATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGCCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGgc                                                      <  2:1392403/150‑1 (MQ=255)
                                                                                                  taataaTCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCt                                                   <  1:800528/150‑1 (MQ=255)
                                                                                                     taatCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGcc                                                >  1:1022826/1‑150 (MQ=255)
                                                                                                       atCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCCCGCGATAGGCGCTGCCGc                                              >  2:321277/1‑150 (MQ=255)
                                                                                                       atCAGACAGGGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGGGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGc                                              <  2:2014058/150‑1 (MQ=255)
                                                                                                          aGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGa                                           >  2:1469321/1‑150 (MQ=255)
                                                                                                           gCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGAc                                          <  2:1879357/150‑1 (MQ=255)
                                                                                                            ccAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGAcc                                         <  1:56246/150‑1 (MQ=255)
                                                                                                            ccAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGAcc                                         <  1:799281/150‑1 (MQ=255)
                                                                                                            ccAGTGCGGCAGGGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGAcc                                         <  1:130668/150‑1 (MQ=255)
                                                                                                              aGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGAttt                                                                                     <  1:87850/104‑1 (MQ=255)
                                                                                                                  gggCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTa                                   >  1:2005322/1‑150 (MQ=255)
                                                                                                                   ggCAGTGTCTACGATCTTTTGAGGGGAAAAAGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCCCAGGCAGGCGATTTGCAGTACGGCAGGAATCGTCACGCGATAGGCGATGCCGCTGACCGCTATaa                                  >  2:1590334/1‑150 (MQ=255)
                                                                                                                     cAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAAcc                                <  2:910684/150‑1 (MQ=255)
                                                                                                                     cAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAAcc                                <  2:1308805/150‑1 (MQ=255)
                                                                                                                      aGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAAccc                               >  2:1338708/1‑150 (MQ=255)
                                                                                                                             aCGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAg                        >  2:136727/1‑150 (MQ=255)
                                                                                                                             aCGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAg                        <  2:1142893/150‑1 (MQ=255)
                                                                                                                              cGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGt                       <  1:1978505/150‑1 (MQ=255)
                                                                                                                              cGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGt                       >  1:2038375/1‑150 (MQ=255)
                                                                                                                               gATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTg                      >  1:924563/1‑150 (MQ=255)
                                                                                                                               gATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGAGCTGCCGCTGACCGCTTTAACCCCATTTAGTg                      >  2:320161/1‑150 (MQ=255)
                                                                                                                               gATCTTTTGAGGGGAAAATGAAAATTTTCCACGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTg                      <  2:408375/150‑1 (MQ=255)
                                                                                                                                aTCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGc                     <  2:223317/150‑1 (MQ=255)
                                                                                                                                  cTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGTGCTGCCGCTGACCGCTTTAACCCCATTTAGTGCCg                   >  1:1624767/1‑150 (MQ=255)
                                                                                                                                      tGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGCCGCAcc               <  1:1472527/150‑1 (MQ=255)
                                                                                                                                        aGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGCCGCACCTa             >  2:2212909/1‑150 (MQ=255)
                                                                                                                                         ggggAAAATTTTCATTTTCCCCGGTTTCAGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGCCGCCCCTAc            >  2:1425074/1‑150 (MQ=255)
                                                                                                                                         ggggAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGCCGCACCTAc            <  2:2089071/150‑1 (MQ=255)
                                                                                                                                         ggggAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGATGCCGCTGACCGCTTTAACCCCATTTAGTGCCGCACCTAc            >  2:1005968/1‑150 (MQ=255)
                                                                                                                                           ggAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGCCGCACCTACAg          >  1:1654896/1‑150 (MQ=255)
                                                                                                                                            gtaaatgaaaatTTTCCCCGGTTTCCGGTATCACTCCGGCGTGGGGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGCCGCACCTACAgg         <  2:638429/148‑1 (MQ=255)
                                                                                                                                             aaaatgaaaatTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGCCGCACCTACAggg        <  1:622886/150‑1 (MQ=255)
                                                                                                                                              aaatgaaaatTTTCCCTGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGCCGCACCTACAGGGc       <  1:909416/150‑1 (MQ=255)
                                                                                                                                              aaatgaaaatTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAAGCCCATTTAGTGCCGCACCTACAggtc       >  2:1634187/1‑148 (MQ=255)
                                                                                                                                               aatgaaaatTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGCCGCACCTACAGGGcc      <  1:1965539/150‑1 (MQ=255)
                                                                                                                                                  gaaaatTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGCCGCACCTACAGGGCCTcc   >  2:1453873/1‑150 (MQ=255)
                                                                                                                                                   aaaatTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAGCCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCGGACCGCTTTAACCCCAGTTAGTGCCGCCCCTACAGGGCCTacc  >  1:1457002/1‑150 (MQ=255)
                                                                                                                                                   aaaatTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGCCGCACCTACAGGGCCTccc  >  1:980054/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
TAGCCAACTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGCTGACCGCTTTAACCCCATTTAGTGCCGCACCTACAGGGCCTCCC  >  NC_000913/10473‑10769

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑