Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 747,900 G→C 6.7% intergenic (‑131/+21) abrB ← / ← ybgO regulator of aidB expression; inner membrane protein/putative fimbrial protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913747,9000GC 6.7% 435.3 / 16.8 143intergenic (‑131/+21)abrB/ybgOregulator of aidB expression; inner membrane protein/putative fimbrial protein
Reads supporting (aligned to +/- strand):  ref base G (68/64);  new base C (7/3);  total (75/68)
Fisher's exact test for biased strand distribution p-value = 3.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.91e-01

Only 100 of 148 total aligned reads displayed.

CCCACTGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGTAGACCAACATGTCACTGGTGAAATCGG  >  NC_000913/747751‑748048
                                                                                                                                                     |                                                                                                                                                    
cccACTGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCcggcg                                                                                                                                                      <  1:657062/150‑1 (MQ=255)
 ccACTGCAAAACTGTCATCCTTCGCTCCCTCATAATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGTGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCcggcgg                                                                                                                                                     <  1:1930992/150‑1 (MQ=255)
 ccACTGCAAAACTGGCATCCTTCGCTCCCTCATTATTGTCTCTAAGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCATTTCcggcgc                                                                                                                                                     >  1:1104118/1‑149 (MQ=255)
    cTGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTATCCGGCGGCaa                                                                                                                                                  >  2:1045646/1‑150 (MQ=255)
      gCAAAACTGGCATCCTTCGCTCCCTCATAATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAAGAAAAGGGGAAAATCAGCAAATTTCTGAAAGAGATGCCCTTTACGGCGGCAAAg                                                                                                                                                >  2:974933/1‑150 (MQ=255)
       cAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAgg                                                                                                                                               >  1:1853869/1‑150 (MQ=255)
          aaCTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGTGCCAGGAAAAAATAAGAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGct                                                                                                                                            <  1:1083107/150‑2 (MQ=255)
            cTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAATTGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTGCCGCCGGAAAGGGcatc                                                                                                                                          <  2:1266335/150‑1 (MQ=255)
            cTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGcatc                                                                                                                                          >  1:50571/1‑150 (MQ=255)
            cTGGCATCCTACGCTCACTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCAGCAAAGGGcatc                                                                                                                                          <  2:285697/150‑1 (MQ=255)
             tGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATATAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAATAGTTGAAAATCAGCAATTTTCTTAAAGAGATGCCCTTTCCGGCGGCAAAGGGcatca                                                                                                                                         <  1:1362119/150‑1 (MQ=255)
             tGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAACGCTGGGTCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGcatca                                                                                                                                         <  1:262210/150‑1 (MQ=255)
              ggCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGcatcat                                                                                                                                        <  2:451925/150‑1 (MQ=255)
               gCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATg                                                                                                                                       <  2:1764541/150‑1 (MQ=255)
                 aTCCTTCGCTCCCTCATTATTTTCTCTGTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGTAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGt                                                                                                                                     >  2:80660/1‑150 (MQ=255)
                    cTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTGTCCGGCGGCAAAGGGCATCATGGTaaa                                                                                                                                  >  2:585883/1‑150 (MQ=255)
                      tCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATc                                                                                                                                >  1:57086/1‑150 (MQ=255)
                        gCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAATGAGATGCCCTATCCGGCCGCAAAGGGCATCATGGTAAATCAg                                                                                                                              >  2:1124210/1‑150 (MQ=255)
                        gCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAg                                                                                                                              <  2:923793/150‑1 (MQ=255)
                        gCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAg                                                                                                                              <  1:2058892/150‑1 (MQ=255)
                             cTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTtata                                                                                                                         <  2:2184504/150‑1 (MQ=255)
                             cTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTtata                                                                                                                         <  2:1089539/150‑1 (MQ=255)
                                attattTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGtt                                                                                                                      <  1:36657/150‑1 (MQ=255)
                                   attTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAAt                                                                                                                   >  2:810017/1‑150 (MQ=255)
                                    ttttCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATc                                                                                                                  <  2:1675044/150‑1 (MQ=255)
                                    ttttCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATc                                                                                                                  <  2:2085100/150‑1 (MQ=255)
                                      ttCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCtt                                                                                                                >  1:256149/1‑150 (MQ=255)
                                       tctcTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTa                                                                                                               <  2:656535/150‑1 (MQ=255)
                                        ctctTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTaa                                                                                                              <  2:698206/150‑1 (MQ=255)
                                          ctTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTaaaa                                                                                                            >  2:1370662/1‑150 (MQ=255)
                                              ggTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGca                                                                                                        >  1:979341/1‑150 (MQ=255)
                                              ggTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGca                                                                                                        <  2:458903/150‑1 (MQ=255)
                                                tgtATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGcacc                                                                                                      <  2:1602270/150‑1 (MQ=255)
                                                   aTCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACt                                                                                                   >  1:1461638/1‑150 (MQ=255)
                                                    tCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTg                                                                                                  <  1:810017/150‑1 (MQ=255)
                                                    tCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTg                                                                                                  <  1:2083892/150‑1 (MQ=255)
                                                     cGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGTGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGt                                                                                                 <  2:1200369/150‑1 (MQ=255)
                                                     cGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGCAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCACTTATAGTTAATCTTAAAAAGCACCACtct                                                                                                 >  2:1743873/1‑148 (MQ=255)
                                                      gATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTa                                                                                                >  2:59370/1‑150 (MQ=255)
                                                        tACAGCATAAAGAAAGCTGGTCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATc                                                                                              >  2:1316298/1‑150 (MQ=255)
                                                            gCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAg                                                                                          <  1:1613087/150‑1 (MQ=255)
                                                            gCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAg                                                                                          >  1:453332/1‑150 (MQ=255)
                                                              aTAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAggg                                                                                        >  2:993031/1‑150 (MQ=255)
                                                               tAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAgggg                                                                                       >  1:601584/1‑150 (MQ=255)
                                                                 aagacagCTGGGCCAGCAAAAAATAACACCAATCAAGGAAAACGCGAAAACCAGCAATTTTCTGAAATAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGcc                                                                                     <  1:2089909/150‑1 (MQ=255)
                                                                   taaagCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAg                                                                                   <  2:1115070/149‑1 (MQ=255)
                                                                      agCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGCGGAAACTCAGCAATTTTCTGAAAGAGATGCCCTTTCCTGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCt                                                                                <  1:557003/150‑1 (MQ=255)
                                                                           ggCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATg                                                                           <  1:296489/150‑1 (MQ=255)
                                                                           ggCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATg                                                                           >  1:1319757/1‑150 (MQ=255)
                                                                            gCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATTCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCTCCACTGTATCGAAGGGGCCAGCCTCGATgg                                                                          >  2:14193/1‑150 (MQ=255)
                                                                                ggAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGt                                                                      >  1:1496539/1‑150 (MQ=255)
                                                                                 taaaaaaTAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTg                                                                     <  2:1961228/149‑1 (MQ=255)
                                                                                 gAAAAAATACCAGCAATCTAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATTGTAAATCAGTTATAGTTTATCTTAAATAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTg                                                                     <  2:1952687/150‑1 (MQ=255)
                                                                                     aaaTAACAGCAATCAAGGAAAATGGGAAAATCAGCAATATTCTGAATGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTATAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTg                                                                 <  1:701655/150‑1 (MQ=255)
                                                                                      aaaaaCAGCAATCTATGAAAAGCGGAAAATCAGCAATTTTCAGAATTAGATGCCCTTTCCGTCGGGAAAGGGTATCATGGTTAATCAGTTATAGCTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTGGATGGGGGTGCCTgg                                                                <  1:387302/147‑1 (MQ=255)
                                                                                        tAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCATGGaa                                                              >  2:1520006/1‑150 (MQ=255)
                                                                                         accAGCAATCACGGACACGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAAt                                                             <  1:771155/148‑1 (MQ=255)
                                                                                         aaCAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAAt                                                             <  2:523725/150‑1 (MQ=255)
                                                                                          aCAGCAATCAAGGAAAGGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATg                                                            <  1:2005099/150‑1 (MQ=255)
                                                                                           cAGCAATCAAGGAACAGCGTTAAATCAGCAATTTTCTGACAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGTGGTTCCTGGAATgg                                                           <  1:114300/150‑1 (MQ=255)
                                                                                           cAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATgg                                                           >  2:70201/1‑150 (MQ=255)
                                                                                           cAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATgg                                                           >  1:724063/1‑150 (MQ=255)
                                                                                            aGCAATCAAGGAAAAGGGGACAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGa                                                          <  1:1106981/150‑1 (MQ=255)
                                                                                            aGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGa                                                          <  1:59370/150‑1 (MQ=255)
                                                                                                aTCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCTGCGGCAAAGGGCATCATGGTAAATAAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGTGGTGCCTGGAATGGCCGaa                                                      >  1:122251/1‑150 (MQ=255)
                                                                                                   aaGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGt                                                   >  2:2159597/1‑150 (MQ=255)
                                                                                                   aaGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGt                                                   >  1:838987/1‑150 (MQ=255)
                                                                                                      gAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCa                                                >  2:1408502/1‑150 (MQ=255)
                                                                                                            gggAAAATCAGCGATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGta                                          >  1:681206/1‑150 (MQ=255)
                                                                                                            gggAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGta                                          <  1:782935/150‑1 (MQ=255)
                                                                                                              gAACATCCGCAATATTCTGAAAGAGCCTCCCATTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTAAAGTTAATCTTAAAAAGCACCACTGTATCTAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGtata                                        <  2:2081208/150‑1 (MQ=255)
                                                                                                              gAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGtata                                        >  2:252046/1‑150 (MQ=255)
                                                                                                               aaaaTCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAg                                       >  2:1646012/1‑150 (MQ=255)
                                                                                                                      gCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGtttttt                                >  1:774179/1‑150 (MQ=255)
                                                                                                                      gCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGAAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGtttttt                                >  2:897614/1‑150 (MQ=255)
                                                                                                                      gCAATTTTCTGAAAGAGATGCCCTATCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGtttttt                                >  2:1687571/1‑150 (MQ=255)
                                                                                                                        aaTTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCg                              <  2:57086/150‑1 (MQ=255)
                                                                                                                         aTTTTCTGAAAGAGATGCCCTTTCAGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGt                             <  1:643065/150‑1 (MQ=255)
                                                                                                                          ttttCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGTa                            <  1:1686356/150‑1 (MQ=255)
                                                                                                                                gAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTGGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGTAGACCaa                      >  1:1388586/1‑150 (MQ=255)
                                                                                                                                 aaaCAGACGCCCATACCGGCGGCAAAGGGCATCATGGAAAATCAGTAAAACTTACTCTACAAAAGCACCACTGTATCGAACGGGCCAGCCGCGATGGGGGGGCCTGGAATGGACGAAAGTTCAGCCGTATAGGGTTTTTCGTAGACCAAc                     <  2:920322/150‑1 (MQ=255)
                                                                                                                                  aaGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGTAGACCAACa                    <  2:77325/150‑1 (MQ=255)
                                                                                                                                    gagaTGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGTAGACCAACATg                  >  2:1043356/1‑150 (MQ=255)
                                                                                                                                    atgaTGCCCTTTGCCGCCGGAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGTAGACCAACATg                  >  1:2061713/3‑150 (MQ=255)
                                                                                                                                      gaTGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGTAGACCAACATGTc                >  2:658695/1‑150 (MQ=255)
                                                                                                                                       aTGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGTAGACCAACATGTCa               >  2:1759759/1‑150 (MQ=255)
                                                                                                                                        tGCCCTTTCCGGCGGCAAAGGGCATCATGGTTAATCAGTTTTAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATCTACGAAAGTTCAGCCCTATAGGTTTTTTCGTAGACCAACATGTCAc              <  1:1264508/150‑1 (MQ=255)
                                                                                                                                         gCCCTTTGCCGCCGGAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCCGCCTCGATGGGGGTGCCTGGAATCGACGAAAGTTCAGCCGTATAGGTTTTTTCGTAGACCAACATGtacct             >  1:1400805/1‑146 (MQ=255)
                                                                                                                                         gCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGTAGACCAACATGTCACt             >  2:249490/1‑150 (MQ=255)
                                                                                                                                         gCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTAATAGTTAATCTTAAAAAGCACCACTGTATCGAAGCGGCCAGCCTCGAGGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGTAGACCAACATGTCACt             <  2:145161/150‑1 (MQ=255)
                                                                                                                                          cccTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGTAGACCAACATGTCACTg            <  2:1599292/150‑1 (MQ=255)
                                                                                                                                          cccTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGTAGACCAACATGTCACTg            >  1:1167944/1‑150 (MQ=255)
                                                                                                                                           ccTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGTAGACCAACATGTCACTgg           >  1:747380/1‑150 (MQ=255)
                                                                                                                                           catttCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCTAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGTAGACCAACATGTCACTgg           >  1:2169481/3‑150 (MQ=255)
                                                                                                                                            cTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGTAGACCAACATGTCACTGGt          <  1:1583464/150‑1 (MQ=255)
                                                                                                                                              ttCCGGCGGCAAAGGTCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGTAGACCAACATGTCACTGGTGa        <  2:601584/150‑1 (MQ=255)
                                                                                                                                              ttCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGTAGACCAACATGTCACTGGTGa        <  2:50571/150‑1 (MQ=255)
                                                                                                                                               tCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGTAGACCAACATGTCACTGGTGaa       <  2:838987/150‑1 (MQ=255)
                                                                                                                                                ccGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGTAGACCAACATGTCACTGGTGaaa      <  2:770692/150‑1 (MQ=255)
                                                                                                                                                    cggcAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCTTATACCAACATGTCACTGGTGaattagg  >  1:2138238/1‑145 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CCCACTGCAAAACTGGCATCCTTCGCTCCCTCATTATTTTCTCTTTGGTGTATCGATACAGCATAAAGAAAGCTGGGCCAGGAAAAAATAACAGCAATCAAGGAAAAGGGGAAAATCAGCAATTTTCTGAAAGAGATGCCCTTTCCGGCGGCAAAGGGCATCATGGTAAATCAGTTATAGTTAATCTTAAAAAGCACCACTGTATCGAAGGGGCCAGCCTCGATGGGGGTGCCTGGAATGGACGAAAGTTCAGCCGTATAGGTTTTTTCGTAGACCAACATGTCACTGGTGAAATCGG  >  NC_000913/747751‑748048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: