Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 794,939 T→C 7.0% intergenic (+17/‑150) acrZ → / → modA AcrAB‑TolC efflux pump accessory protein, membrane‑associated/molybdate ABC transporter periplasmic binding protein; chlorate resistance protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913794,9390TC 7.0% 503.9 / 20.6 170intergenic (+17/‑150)acrZ/modAAcrAB‑TolC efflux pump accessory protein, membrane‑associated/molybdate ABC transporter periplasmic binding protein; chlorate resistance protein
Reads supporting (aligned to +/- strand):  ref base T (83/75);  new base C (9/3);  total (92/78)
Fisher's exact test for biased strand distribution p-value = 2.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.99e-01

Only 100 of 172 total aligned reads displayed.

AAGTCTGGTATTCGCCGTAATCATGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTACATTAAGGGGTTACC  >  NC_000913/794790‑795087
                                                                                                                                                     |                                                                                                                                                    
   tCTGGTATTCGCCGTAATCATGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAg                                                                                                                                                   >  1:2096668/1‑150 (MQ=255)
   gcTGGTATTCGCCGTAATCATGGTACCGGTCGTGAAGCCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGCGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAg                                                                                                                                                   <  2:446238/149‑1 (MQ=255)
      ggTATTCGCCGTAATCATGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCTGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCg                                                                                                                                                >  1:499212/1‑150 (MQ=255)
      ggTATTCGCCGTAATCATGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCg                                                                                                                                                >  2:386844/1‑150 (MQ=255)
        tATTCGCCGTAATCATGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCggg                                                                                                                                              >  1:962800/1‑150 (MQ=255)
         aTTCGCCGTAATCATGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTCGTGTTGGTAAAAAAGACCAGACCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAgaagtga                                                                                                                                             >  2:1524575/1‑144 (MQ=255)
             gCCGTAATCATGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCAttt                                                                                                                                         >  1:86122/1‑150 (MQ=255)
              ccGTAATCATGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGACTAAGCGGGCAttca                                                                                                                                        <  2:108130/150‑3 (MQ=255)
                gTAATCATGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAATTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCAttttct                                                                                                                                      <  2:921370/150‑1 (MQ=255)
                gTAATCATGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGACTAAGCGGGCAttcagg                                                                                                                                      <  1:1841088/150‑5 (MQ=255)
                 tAATCATGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGTTCTTGGTGAAGTATTCAACATCTTTTCTTGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGACTAAGCGGGCAttcaggg                                                                                                                                     <  2:281538/150‑6 (MQ=255)
                        ggTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCa                                                                                                                              <  1:386844/150‑1 (MQ=255)
                        ggTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCAGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCa                                                                                                                              >  2:971781/1‑150 (MQ=255)
                            ccTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACtt                                                                                                                          >  2:2125645/1‑150 (MQ=255)
                             cTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTc                                                                                                                         >  1:1411381/1‑150 (MQ=255)
                             cTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTATCAACTTc                                                                                                                         >  2:1403570/1‑150 (MQ=255)
                              tGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTcc                                                                                                                        >  2:1225770/1‑150 (MQ=255)
                                  gTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCt                                                                                                                    >  2:277331/1‑150 (MQ=255)
                                      tGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCTGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTc                                                                                                                <  2:565235/150‑1 (MQ=255)
                                       ggCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTcc                                                                                                               >  1:1040619/1‑150 (MQ=255)
                                           atcatcCTGGGTCTGTTTTACGGTCTTGGTGAATTATTGAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGcc                                                                                                           <  2:1134375/150‑1 (MQ=255)
                                           atcatcCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGcc                                                                                                           <  1:1003838/150‑1 (MQ=255)
                                                   gggTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATAATTGATTCCCTTAATGCCCTCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTTCCGATAtttt                                                                                                   >  1:201476/1‑150 (MQ=255)
                                                    ggTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATAttttt                                                                                                  >  2:613339/1‑150 (MQ=255)
                                                    ggTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATAttttt                                                                                                  >  1:825079/1‑150 (MQ=255)
                                                    ggTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATAttttt                                                                                                  <  2:1798505/150‑1 (MQ=255)
                                                     gTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATAtttttt                                                                                                 >  1:289106/1‑150 (MQ=255)
                                                     gTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATAtttttt                                                                                                 >  1:1186395/1‑150 (MQ=255)
                                                      gcTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTc                                                                                                <  2:36538/149‑1 (MQ=255)
                                                       cTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCt                                                                                               >  1:910594/1‑150 (MQ=255)
                                                         gATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTa                                                                                             <  2:346856/150‑1 (MQ=255)
                                                         gATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTa                                                                                             >  2:1638095/1‑150 (MQ=255)
                                                         gATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTa                                                                                             >  1:712211/1‑150 (MQ=255)
                                                             tACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGACGATATTTTTTATTATCTa                                                                                         >  1:1509503/1‑150 (MQ=255)
                                                              aCGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTAc                                                                                        >  1:2203697/1‑150 (MQ=255)
                                                                 gTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTc                                                                                     <  2:1777391/150‑1 (MQ=255)
                                                                 gTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTc                                                                                     >  2:1317543/1‑150 (MQ=255)
                                                                 gTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTc                                                                                     >  1:677859/1‑150 (MQ=255)
                                                                     tGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACaa                                                                                 >  1:680333/1‑150 (MQ=255)
                                                                      ggTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTTTCTACCTCACaaa                                                                                >  2:202732/1‑150 (MQ=255)
                                                                       gTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAg                                                                               >  1:807514/1‑150 (MQ=255)
                                                                        tGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAgg                                                                              <  1:1867948/150‑1 (MQ=255)
                                                                        tGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATATACATCACAAAgg                                                                              >  1:1970225/1‑150 (MQ=255)
                                                                         gAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACATCACAAAGGt                                                                             >  2:1063692/1‑150 (MQ=255)
                                                                            gTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAg                                                                          <  2:1883028/150‑1 (MQ=255)
                                                                            gTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAg                                                                          >  1:41059/1‑150 (MQ=255)
                                                                             tATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACGAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGc                                                                         <  1:36209/150‑1 (MQ=255)
                                                                             tATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGc                                                                         <  2:1549262/150‑1 (MQ=255)
                                                                             tATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGc                                                                         <  1:254304/150‑1 (MQ=255)
                                                                                tCAACATCTTTTCTGGTATTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAAt                                                                      <  1:1114560/150‑1 (MQ=255)
                                                                                 cAACATCTTTTCTGGGGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTGCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATa                                                                     >  2:1080709/1‑150 (MQ=255)
                                                                                 cAAAATCTTTTCTGGTGTTTGTAAAAAAGACCAGCACGGAAAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCATTTTCTCAACTTCCTGCATTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATa                                                                     <  1:444120/150‑1 (MQ=255)
                                                                                    cATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAAct                                                                  <  2:1778083/150‑1 (MQ=255)
                                                                                      tCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAActgc                                                                <  1:613339/150‑1 (MQ=255)
                                                                                      tCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAActgc                                                                >  1:1670093/1‑150 (MQ=255)
                                                                                         tttCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGGCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTgg                                                             >  1:1507741/1‑150 (MQ=255)
                                                                                         tttCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTgg                                                             >  1:1563463/1‑150 (MQ=255)
                                                                                          ttCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACATCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTggg                                                            <  2:170725/150‑1 (MQ=255)
                                                                                            cTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGaa                                                          >  1:797392/1‑150 (MQ=255)
                                                                                                 gtTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTc                                                     <  2:870155/150‑1 (MQ=255)
                                                                                                  ttGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTcc                                                    <  1:97960/150‑1 (MQ=255)
                                                                                                   tGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCGTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAAATccc                                                   >  2:1447952/1‑149 (MQ=255)
                                                                                                     gTAAAATAGACCAGCCCGGACAAAATCATTGAATCCCTGAATGCCAGCTTAGTCGGGAATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAg                                                 <  1:382458/150‑1 (MQ=255)
                                                                                                     gTAAAAAAGACCAGCCCTGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAg                                                 <  1:1313462/150‑1 (MQ=255)
                                                                                                     gTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCGGAg                                                 >  1:110997/1‑150 (MQ=255)
                                                                                                     gTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAg                                                 <  1:578410/150‑1 (MQ=255)
                                                                                                       aaaaaaGACCAGCCTGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGtt                                               <  2:499212/150‑1 (MQ=255)
                                                                                                       aaaaaaGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGtt                                               <  2:47456/150‑1 (MQ=255)
                                                                                                       aaaaaaGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCATATCTACCTCACAAATGTTAGCAATAACTGCTGGGTAAATTCCGAGtt                                               >  1:283837/1‑150 (MQ=255)
                                                                                                        aaaaaGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTa                                              <  1:607602/150‑1 (MQ=255)
                                                                                                         aaaaGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAg                                             >  1:2065745/1‑150 (MQ=255)
                                                                                                              aCCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGTCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCATTAACTGCTGGGAAAATTCCGAGTTTGTCGtt                                        <  2:1942333/150‑1 (MQ=255)
                                                                                                              aCCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGtt                                        <  2:693576/150‑1 (MQ=255)
                                                                                                               ccATCCCTGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATATTTTCTTATCTACCTCACTAAGTTTATCAATAACTGCTGGGAAAATTCCGAGTTATTCGTta                                       <  2:736796/150‑1 (MQ=255)
                                                                                                               ccAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGttt                                       >  1:1433472/1‑149 (MQ=255)
                                                                                                               ccAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTta                                       >  1:1388708/1‑150 (MQ=255)
                                                                                                                      ggACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTc                                <  2:1160157/150‑1 (MQ=255)
                                                                                                                      ggACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTc                                <  2:825079/150‑1 (MQ=255)
                                                                                                                       taCAAAATCATTGATTCCCTTAATGCCCGCTTAGTCGGGCATTTTCTTTATCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCg                               <  2:322124/149‑1 (MQ=255)
                                                                                                                       taCAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCg                               <  1:1403570/149‑1 (MQ=255)
                                                                                                                           aaaTCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTa                           >  1:127211/1‑150 (MQ=255)
                                                                                                                            aaTCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTAc                          <  2:2164368/150‑1 (MQ=255)
                                                                                                                                  tGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACg                    >  1:44323/1‑150 (MQ=255)
                                                                                                                                  tGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACg                    <  2:910594/150‑1 (MQ=255)
                                                                                                                                   gATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGt                   >  2:536265/1‑150 (MQ=255)
                                                                                                                                     aaaagaaaaTGCCCGACTAAGCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCAGAGTTAGTCGTTATATTGTCGCCTAGATAACGTTa                 >  1:1085162/8‑150 (MQ=255)
                                                                                                                                       cccTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTACa               >  1:1780750/1‑150 (MQ=255)
                                                                                                                                        ccTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTACAt              >  1:313358/1‑150 (MQ=255)
                                                                                                                                        ccTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTACAt              >  2:1253441/1‑150 (MQ=255)
                                                                                                                                        agaaaaTGCCCGACTAAGCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTACAt              >  2:1451980/5‑150 (MQ=255)
                                                                                                                                        agaaaaTGCCCGACTAAGCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTACAt              >  2:1342707/5‑150 (MQ=255)
                                                                                                                                         cTGAATGCCCGCTTAGTCTGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTACAtt             <  2:1573722/150‑1 (MQ=255)
                                                                                                                                           gAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTACATTaa           <  1:1920007/150‑1 (MQ=255)
                                                                                                                                           gAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTACATTaa           >  1:1483547/1‑150 (MQ=255)
                                                                                                                                             aTGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTACATTAAgg         <  2:1186395/150‑1 (MQ=255)
                                                                                                                                             aTGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTACATTAAgg         <  1:733869/150‑1 (MQ=255)
                                                                                                                                              tGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTACATTAAggg        <  2:712211/150‑1 (MQ=255)
                                                                                                                                               gCCCGCTTAGTCGGTCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTACATTAAgggg       <  1:1225770/150‑1 (MQ=255)
                                                                                                                                                  cGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTATCCTGCCGATATTTATTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTTGGAAAATTCCGAGTTAGTCGTTATATTGTCGCATACATAACGTTACATAAAGGGGTTa    >  2:2150267/1‑150 (MQ=255)
                                                                                                                                                    cTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTACATTAAGGGGTTAcc  <  2:1196339/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
AAGTCTGGTATTCGCCGTAATCATGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTACATTAAGGGGTTACC  >  NC_000913/794790‑795087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: