Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 794,943 T→G 6.9% intergenic (+21/‑146) acrZ → / → modA AcrAB‑TolC efflux pump accessory protein, membrane‑associated/molybdate ABC transporter periplasmic binding protein; chlorate resistance protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913794,9430TG 6.9% 484.7 / 21.5 171intergenic (+21/‑146)acrZ/modAAcrAB‑TolC efflux pump accessory protein, membrane‑associated/molybdate ABC transporter periplasmic binding protein; chlorate resistance protein
Reads supporting (aligned to +/- strand):  ref base T (83/76);  new base G (9/3);  total (92/79)
Fisher's exact test for biased strand distribution p-value = 1.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.58e-01

Only 100 of 171 total aligned reads displayed.

GGTATTCGCCGTAATCATGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTACATTAAGGGGTTACCAATG  >  NC_000913/794796‑795091
                                                                                                                                                   |                                                                                                                                                    
ggTATTCGCCGTAATCATGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCg                                                                                                                                                    >  2:386844/1‑150 (MQ=255)
  tATTCGCCGTAATCATGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCggg                                                                                                                                                  >  1:1883028/1‑150 (MQ=255)
     tggacggaaTGATGGAACCTGTCGCGATGGCCATCATCCTGGGTCTGATGAACGTTCTTGGTGAAGTTTTCAACATCTTTTCTGGTGTTGGGAAAAAAGACCAGCCCGGACAAACTCATTGATTCCCTGAATGCCCGCTTAGTCGGGCAt                                                                                                                                               <  2:1400366/143‑1 (MQ=255)
        ccGTAATCATGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGACTAAGCGGGCAttca                                                                                                                                            <  2:108130/150‑3 (MQ=255)
          gTAATCATGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAATTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCAttttct                                                                                                                                          <  2:921370/150‑1 (MQ=255)
          gTAATCATGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGACTAAGCGGGCAttcagg                                                                                                                                          <  1:1841088/150‑5 (MQ=255)
           tAATCATGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGTTCTTGGTGAAGTATTCAACATCTTTTCTTGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGACTAAGCGGGCAttcaggg                                                                                                                                         <  2:281538/150‑6 (MQ=255)
            aaTCATGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCAttttcttt                                                                                                                                        >  1:2076385/1‑150 (MQ=255)
             aTCATGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAATTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCTGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCAttttctttt                                                                                                                                       >  2:1145885/1‑150 (MQ=255)
             aTCATGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCAttttctttt                                                                                                                                       <  1:1492120/150‑1 (MQ=255)
                  ggTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCa                                                                                                                                  <  1:386844/150‑1 (MQ=255)
                  ggTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCAGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCa                                                                                                                                  >  2:971781/1‑150 (MQ=255)
                   gTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCaa                                                                                                                                 >  1:1239507/1‑150 (MQ=255)
                    tACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCaaa                                                                                                                                >  1:613948/1‑149 (MQ=255)
                      ccTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACtt                                                                                                                              >  2:2125645/1‑150 (MQ=255)
                       cTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTc                                                                                                                             >  1:1411381/1‑150 (MQ=255)
                          tCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTg                                                                                                                          <  1:572099/150‑1 (MQ=255)
                            gTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCt                                                                                                                        >  2:277331/1‑150 (MQ=255)
                              gATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCttt                                                                                                                      <  2:712259/150‑1 (MQ=255)
                                tGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCTGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTc                                                                                                                    <  2:565235/150‑1 (MQ=255)
                                 ggCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTcc                                                                                                                   >  1:1040619/1‑150 (MQ=255)
                                     atcatcCTGGGTCTGTTTTACGGTCTTGGTGAATTATTGAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGcc                                                                                                               <  2:1134375/150‑1 (MQ=255)
                                     atcatcCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGcc                                                                                                               <  1:1003838/150‑1 (MQ=255)
                                          ccTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTATTTTTCTCAACTTCCTGCTTTTCCTGCCGatat                                                                                                          >  2:1207053/1‑150 (MQ=255)
                                             gggTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATAATTGATTCCCTTAATGCCCTCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTTCCGATAtttt                                                                                                       >  1:201476/1‑150 (MQ=255)
                                              ggTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATAttttt                                                                                                      >  2:613339/1‑150 (MQ=255)
                                               gTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATAtttttt                                                                                                     >  1:1186395/1‑150 (MQ=255)
                                               gTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATAtttttt                                                                                                     >  1:289106/1‑150 (MQ=255)
                                                gcTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTc                                                                                                    <  2:36538/149‑1 (MQ=255)
                                                 cTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCt                                                                                                   >  1:910594/1‑150 (MQ=255)
                                                  tGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGTGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCtt                                                                                                  <  1:261051/150‑1 (MQ=255)
                                                  tGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCtt                                                                                                  >  2:1920007/1‑150 (MQ=255)
                                                   gATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTa                                                                                                 <  2:346856/150‑1 (MQ=255)
                                                   gATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTa                                                                                                 >  2:1638095/1‑150 (MQ=255)
                                                       tACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGACGATATTTTTTATTATCTa                                                                                             >  1:1509503/1‑150 (MQ=255)
                                                        aCGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTAc                                                                                            >  1:2203697/1‑150 (MQ=255)
                                                         cGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTAcc                                                                                           >  2:1178429/1‑150 (MQ=255)
                                                           gTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTc                                                                                         >  2:1317543/1‑150 (MQ=255)
                                                             cTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTcac                                                                                       >  2:1598178/1‑150 (MQ=255)
                                                               tGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACaa                                                                                     >  1:680333/1‑150 (MQ=255)
                                                                 gTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAg                                                                                   >  1:807514/1‑150 (MQ=255)
                                                                  tGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAgg                                                                                  <  1:1867948/150‑1 (MQ=255)
                                                                  tGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATATACATCACAAAgg                                                                                  >  1:1970225/1‑150 (MQ=255)
                                                                   gAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGt                                                                                 <  1:825139/150‑1 (MQ=255)
                                                                      gTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAg                                                                              >  1:41059/1‑150 (MQ=255)
                                                                       tATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACGAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGc                                                                             <  1:36209/150‑1 (MQ=255)
                                                                       tATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGc                                                                             <  2:1549262/150‑1 (MQ=255)
                                                                       tATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGc                                                                             <  1:254304/150‑1 (MQ=255)
                                                                          tCAACATCTTTTCTGGTATTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAAt                                                                          <  1:1114560/150‑1 (MQ=255)
                                                                              cATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAAct                                                                      <  2:1778083/150‑1 (MQ=255)
                                                                                tCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAActgc                                                                    >  1:1670093/1‑150 (MQ=255)
                                                                                   tttCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTgg                                                                 >  1:1563463/1‑150 (MQ=255)
                                                                                    ttCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACATCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTggg                                                                <  2:170725/150‑1 (MQ=255)
                                                                                     tCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGa                                                               >  2:1824061/1‑150 (MQ=255)
                                                                                      cTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGaa                                                              >  1:797392/1‑150 (MQ=255)
                                                                                           gtTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTc                                                         <  2:870155/150‑1 (MQ=255)
                                                                                            ttGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTcc                                                        <  1:97960/150‑1 (MQ=255)
                                                                                             tGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCg                                                       <  2:1239507/150‑1 (MQ=255)
                                                                                             tGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCg                                                       >  1:679880/1‑150 (MQ=255)
                                                                                               gTAAAAAAGACCAGCCCTGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAg                                                     <  1:1313462/150‑1 (MQ=255)
                                                                                               gTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGATAATTCCGAg                                                     >  2:467904/1‑150 (MQ=255)
                                                                                               gTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCGGAg                                                     >  1:110997/1‑150 (MQ=255)
                                                                                               gTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAg                                                     <  1:578410/150‑1 (MQ=255)
                                                                                                 aaaaaaGACCAGCCTGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGtt                                                   <  2:499212/150‑1 (MQ=255)
                                                                                                 aaaaaaGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGtt                                                   <  2:47456/150‑1 (MQ=255)
                                                                                                 aaaaaaGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGtt                                                   <  2:1771720/150‑1 (MQ=255)
                                                                                                  aaaaaGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATTACTGCTGGGAAAATTCCGAGttt                                                  >  1:780017/1‑149 (MQ=255)
                                                                                                  aaaaaGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTa                                                  <  1:607602/150‑1 (MQ=255)
                                                                                                   aaaaGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAg                                                 >  1:2065745/1‑150 (MQ=255)
                                                                                                        aCCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGTCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCATTAACTGCTGGGAAAATTCCGAGTTTGTCGtt                                            <  2:1942333/150‑1 (MQ=255)
                                                                                                        aCCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGtt                                            <  2:693576/150‑1 (MQ=255)
                                                                                                         ccATCCCTGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATATTTTCTTATCTACCTCACTAAGTTTATCAATAACTGCTGGGAAAATTCCGAGTTATTCGTta                                           <  2:736796/150‑1 (MQ=255)
                                                                                                         ccAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGttt                                           >  1:1433472/1‑149 (MQ=255)
                                                                                                         ccAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTta                                           >  1:1388708/1‑150 (MQ=255)
                                                                                                           aGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTtata                                         >  1:728566/1‑150 (MQ=255)
                                                                                                                ggACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTc                                    <  2:825079/150‑1 (MQ=255)
                                                                                                                 taCAAAATCATTGATTCCCTTAATGCCCGCTTAGTCGGGCATTTTCTTTATCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCg                                   <  2:322124/149‑1 (MQ=255)
                                                                                                                 taCAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCg                                   <  1:1403570/149‑1 (MQ=255)
                                                                                                                   cAAAATCATTGATTCCCTGAATTCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGcc                                 <  2:1059444/150‑1 (MQ=255)
                                                                                                                       aTCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACa                             <  1:87825/150‑1 (MQ=255)
                                                                                                                            tGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACg                        >  1:44323/1‑150 (MQ=255)
                                                                                                                            tGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACg                        <  2:910594/150‑1 (MQ=255)
                                                                                                                             gATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGt                       <  1:36349/150‑1 (MQ=255)
                                                                                                                             gATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGt                       >  2:536265/1‑150 (MQ=255)
                                                                                                                               aaaagaaaaTGCCCGACTAAGCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCAGAGTTAGTCGTTATATTGTCGCCTAGATAACGTTa                     >  1:1085162/8‑150 (MQ=255)
                                                                                                                                aaagaaaaTGCCCGACTAAGCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTAc                    >  2:1517440/7‑150 (MQ=255)
                                                                                                                                 cccTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTACa                   >  1:1780750/1‑150 (MQ=255)
                                                                                                                                  ccTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTACAt                  >  1:313358/1‑150 (MQ=255)
                                                                                                                                  ccTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTACAt                  >  1:453566/1‑150 (MQ=255)
                                                                                                                                  agaaaaTGCCCGACTAAGCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACATAGGTTAGCAATAACTGCTGGGAAAATTAGGAGTTAGTCTTTATATTGTCGCCTACATAACTTTACAt                  >  2:1405741/5‑150 (MQ=255)
                                                                                                                                  agaaaaTGCCCGACTAAGCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTACAt                  >  2:1791385/5‑150 (MQ=255)
                                                                                                                                  agaaaaTGCCCGACTAAGCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTACAt                  >  2:1451980/5‑150 (MQ=255)
                                                                                                                                  agaaaaTGCCCGACTAAGCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTACAt                  >  2:1342707/5‑150 (MQ=255)
                                                                                                                                  agaaaaTGCCCGACTAAGCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTACAt                  >  2:2195423/5‑150 (MQ=255)
                                                                                                                                     gAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTACATTaa               >  1:1483547/1‑150 (MQ=255)
                                                                                                                                       aTGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTACATTAAgg             <  2:1070410/150‑1 (MQ=255)
                                                                                                                                         gCCCGCTTAGTCGGTCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTACATTAAgggg           <  1:1225770/150‑1 (MQ=255)
                                                                                                                                            cGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTATCCTGCCGATATTTATTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTTGGAAAATTCCGAGTTAGTCGTTATATTGTCGCATACATAACGTTACATAAAGGGGTTa        >  2:2150267/1‑150 (MQ=255)
                                                                                                                                              cTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTACATTAAGGGGTTAcc      <  2:1196339/150‑1 (MQ=255)
                                                                                                                                                  gTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTACATTAAGGGGTTACCAATg  >  1:932260/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GGTATTCGCCGTAATCATGGTACCTGTCGTGATGGCCATCATCCTGGGTCTGATTTACGGTCTTGGTGAAGTATTCAACATCTTTTCTGGTGTTGGTAAAAAAGACCAGCCCGGACAAAATCATTGATTCCCTGAATGCCCGCTTAGTCGGGCATTTTCTTTTTCTCAACTTCCTGCTTTTCCTGCCGATATTTTTTCTTATCTACCTCACAAAGGTTAGCAATAACTGCTGGGAAAATTCCGAGTTAGTCGTTATATTGTCGCCTACATAACGTTACATTAAGGGGTTACCAATG  >  NC_000913/794796‑795091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: