Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 869,724 A→C 5.2% N105T (AAC→ACC)  gsiB → glutathione ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913869,7240AC 5.2% 392.9 / 7.8 141N105T (AAC→ACC) gsiBglutathione ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (70/62);  new base C (2/6);  total (72/69)
Fisher's exact test for biased strand distribution p-value = 1.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 142 total aligned reads displayed.

GAAATCGTTTTACCAGGGGCTGTTCGGTCTGGATAAAGAGATGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGAAGCGATCGATCCGACAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTT  >  NC_000913/869575‑869871
                                                                                                                                                     |                                                                                                                                                   
gAAATCGTTTTACCAGGGGCTGTTCGGTCTGGATAAAGAGATGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCaa                                                                                                                                                     >  2:2190031/1‑150 (MQ=255)
  aaTCGTTTTACCAGGGGCTGTTCGGTCTGGATAAAGAGATGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAAcg                                                                                                                                                   >  2:572942/1‑150 (MQ=255)
   aTCGTTTTACCAGTGGCTGTTCGGTCTGGATAAAGTGATGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAAcgc                                                                                                                                                  <  1:464240/150‑1 (MQ=255)
     cGTTTTACCAGGGGCTGTTCGGTCTGGATAAAGAGATGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAAcgccg                                                                                                                                                <  1:1024970/150‑1 (MQ=255)
        tttACCAGGGGCTGTTCGGTCTGGATAAAGAGATGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCgcgg                                                                                                                                             >  1:710574/1‑150 (MQ=255)
         ttACCAGGGGCTGTTCGGTCTGGATAAAGAGATGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCgcggc                                                                                                                                            >  1:2012222/1‑150 (MQ=255)
           aCCAGGGGCTGTTCGGTCTGGATAAAGAGATGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGcggcgg                                                                                                                                          >  1:358453/1‑150 (MQ=255)
           aCCAGGGGCTGTTCGGTCTGGATAAAGAGATGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGGTTCCGATGACGGCATTACTTACACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGcggcgg                                                                                                                                          <  1:1809683/150‑1 (MQ=255)
           aCCAGGGGCTGTTCGGTCTGGATAAAGAGATGAAACTGAAAAACGTGCTGGCGGAGAGTTATACAGTTTCCGATGACGGCATTACTTACACCGTCAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGcggcgg                                                                                                                                          >  2:1094063/1‑150 (MQ=255)
            ccAGGGGCTGTTCGGTCGGGATAAAGAGATGAAACTGAAAACCGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGt                                                                                                                                         <  2:410610/150‑1 (MQ=255)
             cAGGGGCTGTTCGGTCTGGATAAAGAGATGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCACCGCCGCGGCGTTg                                                                                                                                        <  2:1537861/150‑1 (MQ=255)
              cggggCTGGTCGGTCTGGATAACGAGACGAAACTGAAAAACGTGCGGGCGGCGAGGTATACCGTTTCCGCTGCCGGCATTACTTCCACCGTGACATTGCGGGAAGGCAGTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGa                                                                                                                                       <  1:2032745/149‑1 (MQ=255)
                 ggCTGTTCGGTCTGGATAAAGAGATGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAg                                                                                                                                    <  2:915697/150‑1 (MQ=255)
                   cTGTTCGGTCTGGATAAAGAGATGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCACCGCCGCGGCGTTGAAATCg                                                                                                                                  <  1:822887/150‑1 (MQ=255)
                       tCGGTCTGGATAAAGAGATGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAGTc                                                                                                                              >  1:1838451/1‑150 (MQ=255)
                       tCGGTATTGATAAAGAGATGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCTATTTCAACGCCGCGGCGGTGAAAGCGAATc                                                                                                                              <  1:1484845/150‑1 (MQ=255)
                        cGGTCTGGATAAAGAGATGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCt                                                                                                                             >  1:2190821/1‑150 (MQ=255)
                         ggTCTGGATAAAGAGATGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTg                                                                                                                            >  2:502192/1‑150 (MQ=255)
                          gTCTGGATAAAGAGATGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTgg                                                                                                                           >  2:559502/1‑150 (MQ=255)
                             tGGATAAAGTGATGAAACTGAAAAACGTGCTGGCTGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGAAATTGCGTGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGAGAATCTGGAcc                                                                                                                        <  2:1398390/150‑1 (MQ=255)
                             tGGATAAAGAGATGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGAcc                                                                                                                        <  1:1705668/150‑1 (MQ=255)
                              ggATAAAGAGATGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCg                                                                                                                       >  2:1506241/1‑150 (MQ=255)
                              ggATAAAGAGATGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCg                                                                                                                       >  2:872421/1‑150 (MQ=255)
                                 tAAAGAGATGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGc                                                                                                                    >  1:742700/1‑150 (MQ=255)
                                   aaGAGATGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGccc                                                                                                                  >  1:1199898/1‑149 (MQ=255)
                                   aaGAGATGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGAGAATCTGGACCGGGCCa                                                                                                                  >  2:609714/1‑150 (MQ=255)
                                    agagATGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAg                                                                                                                 >  1:558140/1‑150 (MQ=255)
                                     gagaTGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGAGGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGc                                                                                                                <  2:1300523/150‑1 (MQ=255)
                                      cgaTGAAACTGAAAAACGTGCGGGCGGAGAGGTATACCGTTTCCGATGACGGCATTACTTACACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAAGCTGGACCGGGCCAGCg                                                                                                               <  2:1344951/149‑1 (MQ=255)
                                      agaTGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGcc                                                                                                               >  1:1768006/1‑149 (MQ=255)
                                       gaTGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGa                                                                                                              >  1:837628/1‑150 (MQ=255)
                                         tGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGagc                                                                                                            >  2:1071449/1‑148 (MQ=255)
                                          gAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCAGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATca                                                                                                           >  1:1277330/1‑149 (MQ=255)
                                           aaaCTGAATAACGTGCTGGCGGAGAGTTATAACGTTTCCGATGACGGCATTACTTACACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATAGCACCGATTTCAACGCCGCGGCGGTCAAAGCGAATCTGGACCTGGCCAGCGATACg                                                                                                          >  1:265323/1‑150 (MQ=255)
                                           aaaCTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCg                                                                                                          >  1:784337/1‑150 (MQ=255)
                                            aaCTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCgg                                                                                                         >  1:943957/1‑150 (MQ=255)
                                             aCTGAAAACCGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGc                                                                                                        <  2:2406/150‑1 (MQ=255)
                                             aCTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGc                                                                                                        <  2:772673/150‑1 (MQ=255)
                                               tGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCgc                                                                                                      >  1:1771258/1‑149 (MQ=255)
                                                gAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGaa                                                                                                     >  1:1620804/1‑150 (MQ=255)
                                                       gTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCtt                                                                                              <  2:1734903/150‑1 (MQ=255)
                                                            ggCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACg                                                                                         <  2:1787541/150‑1 (MQ=255)
                                                                 agagTTATACCGTTTCCGATGACGGCATTACTTACACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCtata                                                                                    <  1:1028388/150‑1 (MQ=255)
                                                                    gTTATACCGTTTCCGATGACGGCATTACTTACACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAAcc                                                                                 >  2:1262162/1‑150 (MQ=255)
                                                                     ttATACCGTTTCCGATGACGGCATTACTTACACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGCTCCGGCGAATCATCTTAAACGCTATAACCt                                                                                >  2:1932842/1‑150 (MQ=255)
                                                                     ttATACCGTTTCCGATGACGGCATTACTTACACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCt                                                                                >  2:1235351/1‑150 (MQ=255)
                                                                      aatcccGTTTCCCATGACGGCATCACTTACACCGTGAACTTGCGGGAACGCAGTAAATTCCAGGATGGCACCGATTTCAACGCCCCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTg                                                                               <  1:1592923/146‑1 (MQ=255)
                                                                       ataCCGTTTCCGATGACGGCATTACTTACACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGt                                                                              >  2:823968/1‑150 (MQ=255)
                                                                          ccGTTTCCGATGACGGCATTACTTACACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTAGAACCTGtata                                                                           >  2:1059089/1‑150 (MQ=255)
                                                                            ttttCCGATGACGGCATTACTTACACCGTGAAATTGCTTGTATGCATTAAATTCCAGTATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGTCCAGCGATCCGGCGAATCATCTTAAACGCTATAACGTGTATAAg                                                                         <  2:1798002/149‑1 (MQ=255)
                                                                            gTTTCCGATGACGGCATTACTTACACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCGACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAg                                                                         <  2:1242395/150‑1 (MQ=255)
                                                                                ccGATGACGGCATTACTTACACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGCCCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTAAAAGAatt                                                                     >  1:1207395/1‑149 (MQ=255)
                                                                                 cGATGACGGCATTACTTACACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAatat                                                                    <  1:2020420/150‑1 (MQ=255)
                                                                                  gATGACTGCATTACTTACACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCTCCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATAtt                                                                   <  2:2149635/150‑1 (MQ=255)
                                                                                    tGACGGCATTACTTACACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTATATAAGAATATTGc                                                                 <  1:116970/150‑1 (MQ=255)
                                                                                    tGACGGCATTACTTACACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACAGATTTCAACGACTCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGc                                                                 <  1:201348/150‑1 (MQ=255)
                                                                                     gACGGCATTACTTACACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCt                                                                >  2:65193/1‑150 (MQ=255)
                                                                                      aCGGCATTACTTACACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTa                                                               <  1:65193/150‑1 (MQ=255)
                                                                                      aCGGCATTACTTACACCGGGAAATTGCGGGAAGGCATTAAAATCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTa                                                               <  2:1364690/150‑1 (MQ=255)
                                                                                       cGGCATTACTTACACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTaa                                                              >  1:925199/1‑150 (MQ=255)
                                                                                          cATTACTTACACCGTGAAATTGCGGGAAGGCATTAAATTCCAGTATGGCACCGATTTCAACGCCTCGGCTGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATTTTGCTAAAAc                                                           <  1:252357/150‑1 (MQ=255)
                                                                                              acttacACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCGGTATAAGAATATTGATAAAACGGaa                                                       >  2:1686019/1‑150 (MQ=255)
                                                                                               cttacACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTTTAAGAATATTGCTAAAACGGAAg                                                      <  2:739545/150‑1 (MQ=255)
                                                                                               cttacACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGAAg                                                      <  1:864498/150‑1 (MQ=255)
                                                                                                  acacCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCTGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACTCTAAAACCTGTATAAGAATATTGCTAAAACGGAAGcga                                                   >  2:890837/1‑150 (MQ=255)
                                                                                                    acCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGAAGcgatc                                                 <  2:252881/150‑1 (MQ=255)
                                                                                                    acCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGAAGcgatc                                                 <  1:2178172/150‑1 (MQ=255)
                                                                                                    acCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGAAGcgatc                                                 <  1:698130/150‑1 (MQ=255)
                                                                                                     ccGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGAAGcgatcg                                                >  2:1924066/1‑150 (MQ=255)
                                                                                                         gAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGAAGCGATCGATcc                                            >  1:2187878/1‑150 (MQ=255)
                                                                                                         gAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGAAGCGATCGATcc                                            <  2:1671384/150‑1 (MQ=255)
                                                                                                          aaaTTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGAAGCGATCGATCCg                                           <  1:1639445/150‑1 (MQ=255)
                                                                                                          aaaTTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGAAGCGATCGATCCg                                           >  1:1841151/1‑150 (MQ=255)
                                                                                                           aaTTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGAAGCGATCGATCCGa                                          <  1:79029/150‑1 (MQ=255)
                                                                                                            aTTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAATGCGAATCTGGAACGGGCCAGCGCTCCGGCGCATAATCTTAAACGCTATATCCTGTATAAGAATATTGCTAAAAGGGAAGCGAGCGATCCGAc                                         >  2:420558/1‑150 (MQ=255)
                                                                                                              tGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATGTGAAACGCTATAACAGGTATAAGAATATTGCTAATCCGGTAGCGATCGATCCGACaa                                       >  2:360625/1‑150 (MQ=255)
                                                                                                              tGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGAAGCGATCGATCCGActa                                       >  2:1961804/1‑148 (MQ=255)
                                                                                                               gCGGGAAGGCATAAAATTCCAGGATGGCACCGATTGCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCTGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGAAGCGATCGATCCGACAAc                                      <  1:88212/150‑1 (MQ=255)
                                                                                                                   gAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGAAGCGATCGATCCGACAACGGTa                                  >  1:369474/1‑150 (MQ=255)
                                                                                                                      ggCATTAAATTCCAGGATGTCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATATGGACCGGGACAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGAAGCGATCGATCCGACAACGGTAAAg                               <  2:1164804/150‑1 (MQ=255)
                                                                                                                       gCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGAAGCGATCGATCCGACAACGGTAAAGa                              <  2:366914/150‑1 (MQ=255)
                                                                                                                       gCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGAAGCGATCGATCCGACAACGGTAAAGa                              <  2:1992623/150‑1 (MQ=255)
                                                                                                                         aTTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGAAGCGATCGATCCGACAACGGTAAAGAtt                            >  1:1875983/1‑150 (MQ=255)
                                                                                                                            aacttCCTGGTTGGACCCGATTGCATCGCCGCGGCGGTGAATGCGAATGTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATTACCTGTATAAGAATATTGCTAAATCGTAAGCGATCGATCCGACAACGGTAAAGATTAcc                         <  1:1094063/147‑1 (MQ=255)
                                                                                                                            aaaTACCAGGATGGCACCCATTTCAACGCCGCGGCGGTGAAAGCGAAACTGGACCGGGCCAGCGATCCGTCGAATCAACTTAAACGCAAAAACCAGAATAAGAAAATTGCTAAAACGGAAGCGATCGATCCGACAACCGTAAACATTAcc                         >  1:354042/1‑150 (MQ=255)
                                                                                                                             taTTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGAGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGAAGCGATCGATCCGACAACGGTAAAGATTAccc                        <  1:1543960/149‑1 (MQ=255)
                                                                                                                             aaTTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGAAGCGATCGATCCGACAACGGTAAAGATTAccc                        <  2:862483/150‑1 (MQ=255)
                                                                                                                              aTTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGCCCGGGCCAGCGATCCGGCGAATCCTCTTAAACGCTATAACCTGTATAAGCATATTGCTAACACGGAAGCGATCGATCCGACAACGGTAAAGATTACCCt                       >  1:1344562/1‑150 (MQ=255)
                                                                                                                                tCCATGATGGCAGCGCGTTCACCCGCGCGGCGGTGATAGCGAAGCTCGAACTGGCGAGCGATCCCGAGAATCATCTTAAACGCTATAAACTGTATAATAATATTGCTAAAACGGAACCCATCGATCCGACAACGGTAAAGATTACCCTct                     <  2:100125/150‑2 (MQ=255)
                                                                                                                                  cAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAACACGGAAGCGATCGATCCGCCAACGCTAAAGATTACCCTCaaa                   >  2:369402/1‑150 (MQ=255)
                                                                                                                                        ggCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGAAGCGATCGATCCGACAACGGTAAAGATTACCCTCAAACAGCCg             <  2:276180/150‑1 (MQ=255)
                                                                                                                                         gCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGAAGCGATCGATCCGACCACGGTAAAGATTACCCTCAAACAGCCGt            >  1:1863456/1‑150 (MQ=255)
                                                                                                                                          cACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGAAGCGATCGATCCGACAACGGTAAAGATTACCCTCAAACAGCCGtt           >  2:1140693/1‑150 (MQ=255)
                                                                                                                                             cGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGAAGCGATCGATCCGACAACGGTAAAGATTACCCTCAAACAGCCGTtctc        >  2:836262/1‑150 (MQ=255)
                                                                                                                                             cGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGAAGCGATCGATCCGACAACGGTAAAGATTACCCTCAAACAGCCGTtctc        <  2:837628/150‑1 (MQ=255)
                                                                                                                                              gctttCACCGCCGCGGCGTTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGAAGCGATCGATCCGACAACGGTAAAGATTACCCTCAAACAGCCGTTCTCa       >  1:2090975/3‑150 (MQ=255)
                                                                                                                                               ttttCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGAAGCGATCGATCCGACAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAg      <  1:1038542/149‑1 (MQ=255)
                                                                                                                                               ctttCACCGCCGCGGCGTTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGAAGCGATCGATCCGACAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAg      >  1:1600392/2‑150 (MQ=255)
                                                                                                                                                 ttCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGAAGCGATCGATCCGACAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCg    >  1:633507/1‑150 (MQ=255)
                                                                                                                                                   cAACGCCGCGTCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGAAGCGATCGATCCGACAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGtt  <  1:1732598/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
GAAATCGTTTTACCAGGGGCTGTTCGGTCTGGATAAAGAGATGAAACTGAAAAACGTGCTGGCGGAGAGTTATACCGTTTCCGATGACGGCATTACTTACACCGTGAAATTGCGGGAAGGCATTAAATTCCAGGATGGCACCGATTTCAACGCCGCGGCGGTGAAAGCGAATCTGGACCGGGCCAGCGATCCGGCGAATCATCTTAAACGCTATAACCTGTATAAGAATATTGCTAAAACGGAAGCGATCGATCCGACAACGGTAAAGATTACCCTCAAACAGCCGTTCTCAGCGTT  >  NC_000913/869575‑869871

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑