Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 13,583 T→G 5.1% V474G (GTT→GGT)  dnaK → chaperone Hsp70, with co‑chaperone DnaJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091313,5830TG 5.1% 501.5 / 10.1 160V474G (GTT→GGT) dnaKchaperone Hsp70, with co‑chaperone DnaJ
Reads supporting (aligned to +/- strand):  ref base T (80/71);  new base G (7/2);  total (87/73)
Fisher's exact test for biased strand distribution p-value = 1.83e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 161 total aligned reads displayed.

TGTTCTCTACCGCTGAAGACAACCAGTCTGCGGTAACCATCCATGTGCTGCAGGGTGAACGTAAACGTGCGGCTGATAACAAATCTCTGGGTCAGTTCAACCTAGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCACCATCAAGGCTTCTTCTGGTCTGAACGAAGATGAAATCCAGAAAATGGTACGCGACGCAGAAGCTAACGCC  >  NC_000913/13436‑13731
                                                                                                                                                   |                                                                                                                                                    
tgtTCACTACCGCTGAAGACAACCAGTCTGCCGTAACCAACCATGTGCTGCTCGGTGAACGTAACCGAGAGGCTGTTTACATATCGCTGGGGCAGTACAACCTAGATGGTATCCACCCGGGACCGCGCCGCTGGCCGCTGATCGAAGTTa                                                                                                                                                    >  2:392787/1‑150 (MQ=255)
  ttCTCTACCGCTGAAGACAACCAGTCTGCGGTAACCATCCATGTGCTGCAGGGTGAACGTAAACGTGCGGCTGATAACAAATCTCTGGGTCAGTTCAACCTAGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTAcc                                                                                                                                                  <  1:843321/150‑1 (MQ=255)
    ctctACCGCTGAAGACAACCAGTCTGCGGTAACCATCCATGTGCTGCAGGGTGAACGTAAACGTGCGGCTGATAACAAATCTCTGGGTCAGTTCAACCTAGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCtt                                                                                                                                                >  1:1511222/1‑150 (MQ=255)
    ctctACCGCTGAAGACAACCAGTCTGCGGTAACCATCCATGTGCTGCAGGGTGAACGTAAACGTGCGGCTGATAACAAATCTCTGGGTCAGTTCAACCTAGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCtt                                                                                                                                                >  1:1128335/1‑150 (MQ=255)
    ctctACCGCTGAAGACAACCAGTCTGCGGTAACCATCCATGTGCTGCAGGGTGAACGTAAACGTGCGGCTGATAACAAATCTCTGGGTCAGTTCAACCTAGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCtt                                                                                                                                                >  1:1261307/1‑150 (MQ=255)
     tctACCGCTGAAGACAACCAGTCTGCGGTAACCATCCATGTGCTGCAGGGTGAACGTAAACGTGCGGCTGATAACAAATCTCTGGGTCAGTTCAACCTAGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTc                                                                                                                                               <  2:205084/150‑1 (MQ=255)
       tACCGCTGAAGACAACCAGTCTGCGGTAACCATCCATGTGCTGCAGGGTGAACGTAAACGTGCGGCTGATAACAAATCTCTGGGTCAGTTCAACCTAGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGa                                                                                                                                             >  1:1024292/1‑150 (MQ=255)
         ccGCTGAAGACAACCAGTCTGCGGTAACCATCCATGTGCTGCAGGGTGAACGTAAACGTGCGGCTGATAACAAATCTCTGGGTCAGTTCAACCTAGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGata                                                                                                                                           <  2:2130415/150‑1 (MQ=255)
         ccGCTGAAGACAACCAGTCTGCGGTAACCATCCATGTGCTGCAGGGTGAACGTAAACGTGCGGCTGATAACAAATCTCTGGGTCAGTTCAACCTAGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTAACTTCGata                                                                                                                                           >  1:1675403/1‑150 (MQ=255)
          cGCTGAAGACAACCAGTCTGAGGTAACCATCCATGTGCTGCAGGGTGAACGTAAACGTGCGGCTGATAACAAATCTCTGGGTCAGTTCAACCTAGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGGTAACTTCGatct                                                                                                                                          <  1:1921031/150‑3 (MQ=255)
            cTGAAGACAACCAGTCTGCGGTAACCATCCATGTGCTGCAGGGTGAACGTAAACGTGCTGCTGATAACAAATCTCTGGGTCAGTGCAACCTAGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCg                                                                                                                                        <  2:1194404/150‑1 (MQ=255)
              gAAGACAACCAGTCTGCGGTAACCATCCATGTGCTGCAGGGTGAACGTAAACGTGCGGCTGATAACAAATCTCTGGGTCAGTTCAACCTAGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGAt                                                                                                                                      <  2:1765280/150‑1 (MQ=255)
                 gACAACCAGTCTGCGGTAACCATCCATGTGCTGCAGGGTGAACGTAAACGTGCGGCTGATAACAAATCTCTGGGTCAGTTCAACCTAGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCt                                                                                                                                   >  1:2213769/1‑150 (MQ=255)
                   cAACCAGTCTGCGGTAACCATCCATGTGCTGCAGGGTGAACGTAAACGTGCGGCTGATAACAAATCTCTGGGTCAGTTCAACCTAGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTAGATATCGATGCTGa                                                                                                                                 >  2:1660246/1‑150 (MQ=255)
                   cAACCAGTCTGCGGTAACCATCCATGTGCTGCAGGGTGAACGTAAACGTGCGGCAGCTAACAAATCTCTGGGTCAGTTCAACCTAGATGGTATCAACCCGGCACCGCGCGGCATGGCGCAGATCGAGGGTACGGTCGATATGGATGGTGa                                                                                                                                 >  2:884717/1‑150 (MQ=255)
                     aCCAGTCTGCGGTAACCATCCATGTGCTGCAGGGTGAACGTAAACGTGCGGCTGATAACAAATCTCTGGGTCAGTTCAACCTAGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACg                                                                                                                               <  2:339056/150‑1 (MQ=255)
                      ccAGTCTGCGGTAACCATCCATGTGCTGCAGGGTGAACGTAAACGTGCGGCTGATAACAAATCTCTGGGTCAGTTCAACCTAGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACgg                                                                                                                              >  2:650609/1‑150 (MQ=255)
                       cAGTCTGCGTTAACCATCCATGTGCTGCAGGGTGAACGTAAACGTGCGGCTGATAACAAATCTCTGGGTCAGTTCAACCTAGATGGAATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGt                                                                                                                             >  2:2100049/1‑150 (MQ=255)
                              cGGTAACCATCCATGTGCTGCACGGTCAACGTAAACGTGCGGCTGATAACAAATCTCTGGGTCAGTTCAACCTAGATTGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGc                                                                                                                      <  1:318352/150‑1 (MQ=255)
                               ggTAACCATCCATGTGCTGCAGGGTGAACGTAAACGTGCGGCTGATAACAAATCTCTGGGTCAGTTCAACCTAGATGGTATCAACCCGTCACCCCGCGGCATGCCGCAGATCGAAGTTACCTTCGATAGCGAAGCTGACGGTATCCTGcc                                                                                                                     >  2:441009/1‑149 (MQ=255)
                                gTAACCATCCATGTGCTGCAGGGTGAACGTAAACGTGCGGCTGATAACAAATCTCTGGGTCAGTTCAACCTAGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCAc                                                                                                                    >  1:152488/1‑150 (MQ=255)
                                   aCCATCCATGTGCTGCAGGGTGAACGTAAACGTGCGGCTGATAACAAATCTCTGGGTCAGTTCAACCTAGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGtt                                                                                                                 >  2:170649/1‑150 (MQ=255)
                                    ccatccatGTGCTGCAGGGTGAACGTAAACGTGCGGCTGATAACAAATCTCTGGGTCAGTTCAACCTAGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCAGCCCGttt                                                                                                                >  1:958696/1‑150 (MQ=255)
                                     catccatGTGCTGCAGGGTGAACGTAAACGTGCGGCTGATAACAAATCTCTGGGTCAGTTCAACCTAGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTc                                                                                                               >  1:70164/1‑150 (MQ=255)
                                     catccatGTGCTGCAGGGTGAACGTAAACGTGCGGCTGATAACAAATCTCTGGGTCAGTTCAACCTAGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTc                                                                                                               <  1:1279095/150‑1 (MQ=255)
                                     catccatGTGCTGCAGGGTGAACGTAAACGTGCGGCTGATAACAAAGCTCTGTGTCAGTTCAACCTAGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGTAGTTACCTTCGATATCGATGCTGTCGGTATCCTGCACGTTTc                                                                                                               <  1:242030/150‑1 (MQ=255)
                                      atccatGTGATGAAGGGTGAACGTAAACGTGCGGCTGATAACAAATCTCTGGTTCAGTTCAACCTAGATGGTATCAAGCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTcc                                                                                                              <  2:1216108/150‑1 (MQ=255)
                                         catGTGCTGCAGGGTGAACGTAAACGTGCGGCTGATAACAAATCTCTGGGTCAGTTCACCCTAGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACCGTCTCCTGCACGTTTCcgcg                                                                                                           >  1:1091070/1‑150 (MQ=255)
                                         catGTGCTGCAGGGTGAACGTAAACGTGCGGCTGATAACAAATCTCTGGGTCAGTTCAACCTAGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCcgcg                                                                                                           >  1:1856867/1‑150 (MQ=255)
                                           tgtgCTGCAGGGTGAACGTAAACGTGCGGCTGATAACAAATCTCTGGGTCAGTTCAACCTAGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGaa                                                                                                         <  2:2188456/150‑1 (MQ=255)
                                           tgtgCTGCAGGGTGAACGTAAACGTGCGGCTGATAACAAATCTCTGGGTCAGTTCAACCTAGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGaa                                                                                                         <  1:1205979/150‑1 (MQ=255)
                                             tgctgcAGGGTGAACGTAAACGTGCGGCTGATAACAAATCTCTGGGTCAGTTCAACCTAGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAg                                                                                                       <  2:733512/150‑1 (MQ=255)
                                                tgcAGGGTGAACGTAAACGTGCGGCTGATAACAAATCTCTGGGTCAGTTCAACCTAGATGGTATCAACCCGGCACCGCGCTGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATa                                                                                                    >  2:441832/1‑150 (MQ=255)
                                                tgcAGGGTGAACGTAAACGTGCGGCTGATAACAAATCTCTGGGTCAGTTCAACCTAGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATa                                                                                                    >  2:1714754/1‑150 (MQ=255)
                                                       tGAACGTAAACGTGCGGCTGATAACAAATCTCTGGGTCAGTTCAACCTAGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTAGATATCGATGCTGACGGTATCCTGCACGTTTCAGCGAAAGATAAAAACAg                                                                                             >  1:1107746/1‑150 (MQ=255)
                                                        taaCGTACACGTTCGGCTGATAACAAATCTCTGGGTCTGTTCAACCTAGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGTTCGAAGTTACCTTCGATATCGATGCTGTCGGTATCCTGCACGTTTCCGCGAAAGATAAATACAGc                                                                                            <  1:692998/149‑1 (MQ=255)
                                                         aaCGTAAACGTGCGGCTGATAACAAATCTCTGGGTCAGTTCAACCTAGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACGGCg                                                                                           >  2:1854864/1‑150 (MQ=255)
                                                           cGTAAACGTGCGGCTGATAACAAATCTCTGGGTCAGTTCAACCTAGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGt                                                                                         >  2:289251/1‑150 (MQ=255)
                                                            gTAAACGTGCGGCTGATAACAAATCTCTGGGGCAGTTCAACCTAGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTa                                                                                        <  1:1379001/150‑1 (MQ=255)
                                                              aaaCGTGCGGCTGATAACAAATCTCTGGGTCAGTTCAACCTAGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTaaa                                                                                      >  1:841637/1‑150 (MQ=255)
                                                                  gTGCGGCTGATAACAAATCTCTGGGTCAGTTCAACCTAGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGc                                                                                  <  2:1790733/150‑1 (MQ=255)
                                                                   tGCGGCTGATAACAAATCTCTGGGTCAGTTCAACCTAGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCa                                                                                 <  1:596266/150‑1 (MQ=255)
                                                                   ggCGGCTGATAACACATCTCTGGGTCAGTTCAACCTAGATGGTATCAACCCGTCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCa                                                                                 <  1:393852/149‑1 (MQ=255)
                                                                       gCTGATAACAAATCTCTGGGTCAGATCAACCTAGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCagaag                                                                             <  2:266124/150‑1 (MQ=255)
                                                                            tAACAAATCTCTGGGTCAGTTCAACCTAGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGAacac                                                                        >  1:506385/1‑150 (MQ=255)
                                                                            tAACAAATCTCTGGGTCAGTTCAACCTAGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCAc                                                                        <  1:1909280/150‑1 (MQ=255)
                                                                             aaCAAATCTCTGGGTCAGTTCAACCTAGATGGTATCAACCCGGCCCCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCAcc                                                                       >  2:636852/1‑150 (MQ=255)
                                                                             aaCAAATCTCTGGGTCAGTTCAACCTAGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTTTCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGAAAAGAGCAGAAGATCAcc                                                                       >  2:1375548/1‑150 (MQ=255)
                                                                             aaCAAATCTCTGGGTCAGTTCAACCTAGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCAcc                                                                       <  2:1739462/150‑1 (MQ=255)
                                                                             aaCAAATCTCTGGGTCAGTTCAACCTAGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCAcc                                                                       <  1:1669898/150‑1 (MQ=255)
                                                                               cAAATCTCTGGGTCAGTTTAACCTAGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCACCAt                                                                     >  1:1236339/1‑150 (MQ=255)
                                                                               cAAATCTCTGGGTCAGTTCAACCTAGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCACCAt                                                                     >  1:526872/1‑150 (MQ=255)
                                                                                      ctGGGTCAGTTCAACCTAGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCACCATCAAGGct                                                              >  2:1112488/1‑150 (MQ=255)
                                                                                      ctGGGTCAGTTCAACCTAGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCACCATCAAGGct                                                              >  1:86201/1‑150 (MQ=255)
                                                                                       tGGGTCAGTTCAACCTAGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCACCATCAAGGctt                                                             >  2:2024349/1‑150 (MQ=255)
                                                                                         ggTCAGTTCAACCTAGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCACCATCAAGGcttct                                                           >  1:1797318/1‑150 (MQ=255)
                                                                                         ggTCAGTTCAACCTAGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCACCATCAAGGcttct                                                           >  1:1552382/1‑150 (MQ=255)
                                                                                           gcAGTTCAACCTAGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCACCATCAATGCttcttc                                                         <  2:1291015/149‑1 (MQ=255)
                                                                                               ttCAACCTAGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCACCATCAAGGCTTCTTCTGGt                                                     <  2:502674/150‑1 (MQ=255)
                                                                                                  aaCCTAGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACCGCGGTAAAGCGCAGAAGATCACCATCAAGGCTTCTTCTGGTCTg                                                  >  1:1605897/1‑150 (MQ=255)
                                                                                                  aaCCTAGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCACCATCAAGGCTTCTTCTGGTCTg                                                  <  2:2108142/150‑1 (MQ=255)
                                                                                                   aCCTAGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCCCCATCACGGCTTCTTCTGGTCTGa                                                 >  1:395396/1‑150 (MQ=255)
                                                                                                   aCCTAGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCACCATCAAGGCTTCTTCTGGTCTGa                                                 <  2:1106372/150‑1 (MQ=255)
                                                                                                    ccTAGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCACCATCAAGGCTTCTTCTGGTCTGaa                                                >  1:1615154/1‑150 (MQ=255)
                                                                                                     cTAGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCACCATCAAGGCTTCTTCTGGTCTGaat                                               <  1:289251/150‑2 (MQ=255)
                                                                                                       aGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGCTTACGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCACCATCAAGGCTTCTTCTGGTCTGAACGa                                             <  2:1394084/150‑1 (MQ=255)
                                                                                                       aGATGGTATAAACCCGGCACCGCGCGGCATCCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAACACAACGGTAAAGAGCAGAAGATCACCATCAAGGCTTCTTCAGGTCAGAACGa                                             >  1:1951883/1‑150 (MQ=255)
                                                                                                        gATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCACCATCAAGGCTTCTTCTGGTCTGAACGaa                                            <  2:1626113/150‑1 (MQ=255)
                                                                                                        gATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCACCATCAAGGCTTCTTCTGGTCTGAACGaa                                            <  2:1014889/150‑1 (MQ=255)
                                                                                                         aTGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCACCATCAAGGCTTCTTCTGGTCTGAACGAAg                                           <  2:1024292/150‑1 (MQ=255)
                                                                                                          tGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCACCATCAAGGCTTCTTCTGGTCTGAACGAAGa                                          >  2:765655/1‑150 (MQ=255)
                                                                                                            gTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAATATAAAAACAGCGGTAAAGAGCAGAAGATCACCATCAAGGCTTCTTCTGGTCTGAACGAAGATg                                        <  1:236629/150‑1 (MQ=255)
                                                                                                            gTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCACCATCAAGGCTTCTTCTGGTCTGAACGAAGATg                                        <  2:1968663/150‑1 (MQ=255)
                                                                                                            gTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCACCATCAAGGCTTCTTCTGGTCTGAACGAAGATg                                        >  1:766527/1‑150 (MQ=255)
                                                                                                               tCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGATAGATAAAAAGAGCGGTAAAGAGCAGAAGATCACCATCCAGGCTTCTTCTGGTCTGCACGATGATGaaa                                     >  1:334581/1‑150 (MQ=255)
                                                                                                                  aCCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCACCATCAAGGCTTCTTCTGGTCTGAACGAAGATGAAATcc                                  <  2:147417/150‑1 (MQ=255)
                                                                                                                  aCCCGGCACCGCGCGGCATGACGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCACCATCAAGGCTTCTTCTGGTCTGAACGAAGATGAAATcc                                  <  1:336273/150‑1 (MQ=255)
                                                                                                                      ggCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCACCATCAAGGCTTCTTCTGGTCTGAACGAAGATGAAATCCAGaa                              <  1:1942847/150‑1 (MQ=255)
                                                                                                                       gCACCGCGCGGCATGCCGCAGATCGATGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCACCATCAAGGCTTCTTCTGGTCTGATCGAAGATGAAATCCAGaaa                             >  1:1373053/1‑150 (MQ=255)
                                                                                                                       gCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCACCATCAAGGCTTCTTCTGGTCTGAACGAAGATGAAATCCAGaaa                             >  1:2092994/1‑150 (MQ=255)
                                                                                                                          ccGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCACCATCAAGGCTTCTTCTGGTCTGAACGAAGATGAAATCCAGAAAATg                          >  1:2121635/1‑150 (MQ=255)
                                                                                                                           cgcgcgGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCACCATCAAGGCTTCTTCTGGTCTGAACGAAGATGAAATCCAGAAAATgg                         >  2:369010/1‑150 (MQ=255)
                                                                                                                                ggCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCACCATCAAGGCTTCTTCTGGTCTGAACGAAGATGAAATCCAGAAAATGGTAcgc                    <  2:1511222/150‑1 (MQ=255)
                                                                                                                                     gCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCACCATCAAGGCTTCTTCTGGTCTGAACGAAGATGAAATCCAGAAAATGGTACGCGACGc               >  1:649669/1‑150 (MQ=255)
                                                                                                                                     gCCGCAGAGCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCTCGTTTCCGCGAAATATAAAAACAGCGGTAAAGAGCAGAAGATCACCATCAAGGCTTCTTCTGGTCTGAACGAAGATGAAATCCAGAAAAGGGTACGCGACGc               <  2:932646/150‑1 (MQ=255)
                                                                                                                                     catcgataTCGAAGGTAACTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCACCATCAAGGCTTCTTCTGGTCTGAACGAAGATGAAATCCAGAAAATGGTACGCGACGc               >  2:1438631/8‑150 (MQ=255)
                                                                                                                                     catcgataTCGAAGGTAACTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCACCATCAAGGCTTCTTCTGGTCTGAACGAAGATGAAATCCAGAAAATGGTACGCGACGc               >  1:453353/8‑150 (MQ=255)
                                                                                                                                     catcgataTCGAAGGTAACTTCGATATCGATGCGGCCGGTATCCTGCACGTTTCCGCGAAAGATAAAACCAGCGGTAAAGAGCAGAAGCTCCCCATCAAGGCTTCTTCTGGTCTGCACGAAGATGAAATCCAGAAAATGGTACGCGACGc               >  2:1645270/8‑150 (MQ=255)
                                                                                                                                       cGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCTGAAGATCACCATCAAGGCTTCTTCTGGTCTGAACGAAGATGAAATCCAGAAAATGGTACGCGACGCAg             <  2:2087076/150‑1 (MQ=255)
                                                                                                                                       cGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCACCATCAAGGCTTCTTCTGGTCTGAACGAAGATGAAATCCAGAAAATGGTACGCGACGCAg             <  2:164883/150‑1 (MQ=255)
                                                                                                                                        cgataTCGAAGGTAACTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCACCATCAAGGCTTCTTCTGGTCTGAACGAAGATGAAATCCAGAAAATGGTACGCGACGCAGa            >  2:668110/5‑150 (MQ=255)
                                                                                                                                          ataTCGAAGGTAACTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCACCATCAAGGCTTCTTCTGGTCTGAACGAAGATGAAATCCAGAAAATGGTACGCGACGCAGAAg          >  2:2203488/3‑150 (MQ=255)
                                                                                                                                          ataTCGAAGGTAACTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCACCATCAAGGCTTCTTCTGGTCTGAACGAAGATGAAATCCAGAAAATGGTACGCGACGCAGAAg          >  2:1676748/3‑150 (MQ=255)
                                                                                                                                           gATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGGTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCACCATCAAGGCTTCTTCTGGTCTGAACGAAGATGAAATCCAGAAAATGGTACGCGACGCAGAAGc         <  2:1209022/150‑1 (MQ=255)
                                                                                                                                              cGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCACCATCAAGGCTTCTTCTGGTCTGAACGAAGATGAAATCCAGAAAATGGTACGCGACGCAGAAGCTaa      <  1:1303678/150‑1 (MQ=255)
                                                                                                                                              cGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCACCATCAAGGCTTCTTCTGGTCTGAACGAAGATGAAATCCAGAAAATGGTACGCGACGCAGAAGCTaa      >  1:286862/1‑150 (MQ=255)
                                                                                                                                              cGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCACCATCAAGGCTTCTTCTGGTCTGAACGAAGATGAAATCCAGAAAATGGTACACGACGCAGAAGCTaa      <  2:563925/150‑1 (MQ=255)
                                                                                                                                               gAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCACCATCAAGGCTTCTTCTGGTCTGAACGAAGATGAAATCCAGAAAATGGTACGCGACGCAGAAGCTAAc     >  1:1012873/1‑150 (MQ=255)
                                                                                                                                                aaGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCACCATCAAGGCTTCTTCTGGTCTGAACGAAGATGAAATCCAGAAAATGGTACGCGACGCAGAAGCTAACg    >  1:773473/1‑150 (MQ=255)
                                                                                                                                                  gTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCACCATCAAGGCTTCTTCTGGTCTGAACGAAGATGAAATCCAGAAAATGGTACGCGACGCAGAAGCTAACGcc  <  1:650609/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TGTTCTCTACCGCTGAAGACAACCAGTCTGCGGTAACCATCCATGTGCTGCAGGGTGAACGTAAACGTGCGGCTGATAACAAATCTCTGGGTCAGTTCAACCTAGATGGTATCAACCCGGCACCGCGCGGCATGCCGCAGATCGAAGTTACCTTCGATATCGATGCTGACGGTATCCTGCACGTTTCCGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCACCATCAAGGCTTCTTCTGGTCTGAACGAAGATGAAATCCAGAAAATGGTACGCGACGCAGAAGCTAACGCC  >  NC_000913/13436‑13731

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: