Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,104,932 C→T 5.4% intergenic (+30/‑29) csgA → / → csgC curlin subunit, amyloid curli fibers, cryptic/curli assembly protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,104,9320CT 5.4% 536.7 / 11.4 167intergenic (+30/‑29)csgA/csgCcurlin subunit, amyloid curli fibers, cryptic/curli assembly protein
Reads supporting (aligned to +/- strand):  ref base C (72/84);  new base T (2/7);  total (74/93)
Fisher's exact test for biased strand distribution p-value = 1.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.66e-01

Only 100 of 171 total aligned reads displayed.

ATGACGGTTAAACAGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCAGAG  >  NC_000913/1104783‑1105081
                                                                                                                                                     |                                                                                                                                                       
aTGACGGTTAAACAGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGgcgc                                                                                                                                                         >  1:2017267/1‑150 (MQ=255)
 tGACTGTTAAACAGTGCGGTGGTGGCAACTGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCa                                                                                                                                                        <  2:733823/150‑1 (MQ=255)
   aCGGTTAAACAGTTCGTTGGTGGAAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGATTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAg                                                                                                                                                      <  1:1970319/150‑1 (MQ=255)
   aCGGTTAAACAGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAg                                                                                                                                                      >  1:1926733/1‑150 (MQ=255)
    cGGTTAAACAGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGc                                                                                                                                                     >  1:1241624/1‑150 (MQ=255)
    cGGTTAAACAGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGc                                                                                                                                                     >  1:1707809/1‑150 (MQ=255)
    cGGTTAAACAGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGc                                                                                                                                                     >  2:545029/1‑150 (MQ=255)
          aaCAGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCTTGCGCCCTG‑‑tt                                                                                                                                             <  2:1236040/150‑1 (MQ=255)
            cAGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACTTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑tttt                                                                                                                                           <  2:1943764/150‑1 (MQ=255)
            cAGTTCGGTGGTGGCAACGGTGCGGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGGTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCTTGCGCCCTG‑‑tttc                                                                                                                                           <  1:870233/150‑2 (MQ=255)
            cAGTGCGGTTGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCTTCAACGTGACTCAGGTTGGCTTTTGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCTTGCGCCCTG‑‑tttc                                                                                                                                           <  1:1901726/150‑2 (MQ=255)
             aGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCTACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑ttttt                                                                                                                                          >  1:80473/1‑150 (MQ=255)
             aGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCTTGCGCCCTG‑‑tttct                                                                                                                                          <  1:1596410/150‑3 (MQ=255)
             aGTTCGGTGGGGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCTTGCGCCCTG‑‑tttct                                                                                                                                          <  2:2056492/150‑3 (MQ=255)
                gcGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑tttttttt                                                                                                                                       <  1:597841/149‑1 (MQ=255)
                   gttgaggCCACGGGGCTGCAGTTGACCAGACTGGATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCGCATCAGTACTACTACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCgg                                                                                                                                    <  2:170277/145‑1 (MQ=255)
                   gtggtgGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCgg                                                                                                                                    <  2:1480075/150‑1 (MQ=255)
                    tggtggCAACGGTGATGAATTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGAATCAGGTTGGCTTTGGTAACAACGCGACCGCTCTTCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCggg                                                                                                                                   <  2:787350/150‑1 (MQ=255)
                     ggtggCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGga                                                                                                                                  >  2:1988129/1‑150 (MQ=255)
                           aaCGGTGCTGCAGGTGACCAGACTGCATCTAACTCCTCCGGCAACGGGACTCCGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAgaagaa                                                                                                                            <  2:207869/150‑1 (MQ=255)
                            aCGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAat                                                                                                                           <  1:753900/150‑1 (MQ=255)
                                  ctgcAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAAt                                                                                                                     >  1:1103549/1‑150 (MQ=255)
                                    gcAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGAAACAACGCGACCGCTCATCAGTACTAATACATCATGTGTATTACAGAAACAGGGCTCAAGCCCTG‑‑TTTGTATACTCGAGAACAATATGACTaa                                                                                                                   >  2:959319/1‑149 (MQ=255)
                                     cAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTAGGGAGAAGAATATGAATACg                                                                                                                  >  1:1253061/1‑150 (MQ=255)
                                       gTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGtt                                                                                                                >  2:887855/1‑150 (MQ=255)
                                       gTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGtt                                                                                                                <  1:1802430/150‑1 (MQ=255)
                                        ttGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAAAACGtta                                                                                                               >  2:1950872/1‑150 (MQ=255)
                                          gACCAGACTGCATCTACCTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTACCAACGCGCCCGCTCATCAGTACTAATACATCATTTGTATTTCAGAAACAGGGCGCAAGCCCTG‑‑TGTTTTTTCGGGAGAAGAATATGAATACGttatt                                                                                                             >  2:2107045/1‑150 (MQ=255)
                                          gACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGttatt                                                                                                             <  1:1318136/150‑1 (MQ=255)
                                          gACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGttatt                                                                                                             >  2:628484/1‑150 (MQ=255)
                                          gACCAGACTGCATCTAACTCCTCCGTCAAAGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCATG‑‑TTTTTTTTCGTGAGAAGAATATGAATACGttatt                                                                                                             >  1:916544/1‑150 (MQ=255)
                                                aCTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCt                                                                                                       >  1:1583832/1‑150 (MQ=255)
                                                   gCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGc                                                                                                    >  1:2020111/1‑150 (MQ=255)
                                                   gCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGc                                                                                                    <  2:1099672/150‑1 (MQ=255)
                                                     aTCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCgg                                                                                                  <  2:1726829/150‑1 (MQ=255)
                                                      tCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGc                                                                                                 <  2:1289460/150‑1 (MQ=255)
                                                      tCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGc                                                                                                 <  2:1064917/150‑1 (MQ=255)
                                                       cTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCa                                                                                                >  2:540143/1‑150 (MQ=255)
                                                        tgaCTCCTCCGTCAACGTGACTCAGGTTGGCTATGGTAACAACGCGACCGCTCATCAGTACTAATACATAATTTGTATTACAGAAACAGGGCTCAAGCCCTG‑‑TTATTTTTAGGGATCAGAATATGAATACGTTATTACTCATTGCGGCAc                                                                                               >  1:129128/3‑150 (MQ=255)
                                                        tAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCAc                                                                                               <  1:1525469/150‑1 (MQ=255)
                                                         aaCTCCTCCGTCAACGTGACTCAGGGTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTTTTACAGAAACAGGGCGCAAGCCCTG‑‑GTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCaat                                                                                              >  1:1776678/1‑148 (MQ=255)
                                                           ctcctcCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAATCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACttt                                                                                            >  2:1609339/1‑150 (MQ=255)
                                                            acctccGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTc                                                                                           <  2:125741/149‑1 (MQ=255)
                                                               cccGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATCCATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAg                                                                                        <  1:1731189/149‑1 (MQ=255)
                                                               accGTCCACGGGACCCCGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGACACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAg                                                                                        <  1:969687/149‑1 (MQ=255)
                                                                 cGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCAAGTc                                                                                      >  2:2010135/1‑150 (MQ=255)
                                                                   tCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAg                                                                                    <  2:1764132/150‑1 (MQ=255)
                                                                   tCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAg                                                                                    <  1:2202136/150‑1 (MQ=255)
                                                                   tCAACGTGACTCAGGTAGGCTTTGGTAACAACGCGACCGCTCATCAGTTATAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAg                                                                                    <  2:459676/150‑1 (MQ=255)
                                                                    cAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGa                                                                                   <  2:1856983/150‑1 (MQ=255)
                                                                     aaCGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACATTATTAATCCTTGCGGCACTTTCCAGTCAGAt                                                                                  >  2:1774037/1‑150 (MQ=255)
                                                                            cTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCttt                                                                           <  1:1064438/150‑1 (MQ=255)
                                                                            cTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCttt                                                                           >  1:2112078/1‑150 (MQ=255)
                                                                            atctggTTGGCTTTGGTAAGAACGCGACCTCTCATCAGTACTTATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTGGGGAGAAGAATATGAATACGTTATTACTCCTTGCTGCACTTTCCAGTCAGATAACCttt                                                                           <  1:1496928/146‑1 (MQ=255)
                                                                              cAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTaa                                                                         >  1:2141197/1‑150 (MQ=255)
                                                                                ggTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATa                                                                       <  2:939036/150‑1 (MQ=255)
                                                                                  ttGGCTTTGGGAACCACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACACGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACg                                                                     <  2:221633/150‑1 (MQ=255)
                                                                                       tttGGTAACAACGCGACCGCTCATCAGTACTAATACCTCAGTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGTTAGAATAATATGTCTACGTTATTACTCCGTGCGGCACTTTCCAGTCAGATAACCTTTAAGACGAcccc                                                                >  2:1581377/1‑149 (MQ=255)
                                                                                        ttGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAg                                                               <  2:2032725/150‑1 (MQ=255)
                                                                                         tGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGc                                                              <  2:1671639/150‑1 (MQ=255)
                                                                                          ggTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCa                                                             >  1:377965/1‑150 (MQ=255)
                                                                                            tAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAg                                                           >  2:1544854/1‑150 (MQ=255)
                                                                                              acaacGCGTACTCTCATCATTACTAATACTTCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAg                                                           <  2:180072/150‑1 (MQ=255)
                                                                                              acaacGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAggg                                                         <  1:978374/150‑1 (MQ=255)
                                                                                              acaacGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAggg                                                         <  2:302322/150‑1 (MQ=255)
                                                                                                aacGCGACCGCTCATCAGTACTAATAAATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGa                                                       >  1:1399400/1‑150 (MQ=255)
                                                                                                  cgcgACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGAtg                                                     <  2:968958/150‑1 (MQ=255)
                                                                                                    cgACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGAtgtg                                                   <  2:569634/150‑1 (MQ=255)
                                                                                                      cccGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAATAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGta                                                 <  1:926607/149‑1 (MQ=255)
                                                                                                      aCCGGTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGACACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGta                                                 <  1:1613728/150‑1 (MQ=255)
                                                                                                       ccGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGtat                                                >  2:1774670/1‑150 (MQ=255)
                                                                                                         gCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATAc                                              <  1:1988129/150‑1 (MQ=255)
                                                                                                               caGTACTAATACATCATATGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCattat                                        <  1:463073/150‑1 (MQ=255)
                                                                                                                   aCTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAAAACGACCCAGCAAGGGGATGTGTATACCATAAATCCt                                    >  2:640884/1‑150 (MQ=255)
                                                                                                                    cTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTg                                   >  1:1874602/1‑150 (MQ=255)
                                                                                                                     tAATACATCATTTGTATTACAGAAACAGGGAGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTGCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGa                                  <  2:1037743/150‑1 (MQ=255)
                                                                                                                      aaTACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGaa                                 >  1:948170/1‑150 (MQ=255)
                                                                                                                      aaTACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACACAGCAAGGGGATGTGTATACGATTATTCCTGaa                                 >  2:1324624/1‑150 (MQ=255)
                                                                                                                      aaTACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACCTTATTACTCCATGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTAAACCATTATTCATGaa                                 >  2:1188588/1‑150 (MQ=255)
                                                                                                                       aTACATGATTTGTATTACAGAAACAGGTCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAg                                <  1:706539/150‑1 (MQ=255)
                                                                                                                       aTACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAg                                <  1:800818/150‑1 (MQ=255)
                                                                                                                           atcatTTGTATTACAGAAACAGGGCGAAAGCCCTT‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCATTCAGATAACCTTTTATACGACCCAGCCAGGGGATGTGTATACCATTATTCCTGAAGTCAc                            <  2:1487815/150‑1 (MQ=255)
                                                                                                                            tcatTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATATCCTTTAATACGACCCATCAAGGGGATGTGTATAACATTATTCCTGAAGTCAct                           >  2:94092/1‑150 (MQ=255)
                                                                                                                              atTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCActct                         <  2:514791/150‑1 (MQ=255)
                                                                                                                               tttGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCtt                        <  2:397498/150‑1 (MQ=255)
                                                                                                                                   tATTACAGAAACAGGGCGCAAGCCCTT‑‑TTTTTTTTAGGGAGAAGAATATTAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTc                    <  2:222035/150‑1 (MQ=255)
                                                                                                                                    cgaaaaaaaaCAGGGCTTGCGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTGTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCa                   >  2:933326/8‑150 (MQ=255)
                                                                                                                                    aTTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGTATGTGTATACCATTATTCCTGAAGTCACTCTTAATCa                   >  1:1551199/1‑150 (MQ=255)
                                                                                                                                     ttACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCaa                  >  1:2136941/1‑150 (MQ=255)
                                                                                                                                       aaaaaaaCAGGGCTTGCGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATc                >  2:147006/5‑150 (MQ=255)
                                                                                                                                          gAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCACTCTTg             >  1:987247/1‑150 (MQ=255)
                                                                                                                                            aaCATGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGGCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACAATTATTCCTGAAGTCACTCTTACTCAATCTTGTc           <  2:1885938/150‑1 (MQ=255)
                                                                                                                                            aaCACGGCGCAAGCCCTT‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTAGTACTCCTTGCGGCACTTTCCAGTCAGATACCCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTc           <  2:350149/150‑1 (MQ=255)
                                                                                                                                             aCAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCt          <  2:881316/150‑1 (MQ=255)
                                                                                                                                              cAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCtg         >  1:631141/1‑150 (MQ=255)
                                                                                                                                              cAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCtg         >  1:484255/1‑150 (MQ=255)
                                                                                                                                                gggCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCtgtg       >  1:84908/1‑150 (MQ=255)
                                                                                                                                                    gcAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTTTCTGTGCaga   >  1:194295/1‑150 (MQ=255)
                                                                                                                                                    gcAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTACACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCaga   >  1:1859105/1‑150 (MQ=255)
                                                                                                                                                     cAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCagag  <  2:2020111/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                       
ATGACGGTTAAACAGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCAGAG  >  NC_000913/1104783‑1105081

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: