Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,104,934 A→C 5.4% intergenic (+32/‑27) csgA → / → csgC curlin subunit, amyloid curli fibers, cryptic/curli assembly protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,104,9340AC 5.4% 511.1 / 13.9 168intergenic (+32/‑27)csgA/csgCcurlin subunit, amyloid curli fibers, cryptic/curli assembly protein
Reads supporting (aligned to +/- strand):  ref base A (74/85);  new base C (2/7);  total (76/92)
Fisher's exact test for biased strand distribution p-value = 1.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.00e-01

Only 100 of 171 total aligned reads displayed.

ACGGTTAAACAGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCAGAGTA  >  NC_000913/1104786‑1105083
                                                                                                                                                    |                                                                                                                                                       
aCGGTTAAACAGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAg                                                                                                                                                        >  1:1926733/1‑150 (MQ=255)
 cGGTTAAACAGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGc                                                                                                                                                       >  1:1241624/1‑150 (MQ=255)
 cGGTTAAACAGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGc                                                                                                                                                       >  1:1707809/1‑150 (MQ=255)
   gTTAAACAGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGccc                                                                                                                                                     <  1:940879/150‑1 (MQ=255)
       aaCAGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTTCAGAAACAGGGCGCAAGCCCTG‑‑tt                                                                                                                                               >  1:39919/1‑150 (MQ=255)
       aaCAGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCTTGCGCCCTG‑‑tt                                                                                                                                               <  2:1236040/150‑1 (MQ=255)
         cAGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACTTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑tttt                                                                                                                                             <  2:1943764/150‑1 (MQ=255)
         aaGTTCGGTGGTGGCAACGGTGCTGCAGTTGACGAGACAGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGTCCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCTTGCGCCCTG‑‑tttc                                                                                                                                             <  2:305396/149‑2 (MQ=255)
          aGTTCGGTGGGGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCTTGCGCCCTG‑‑tttct                                                                                                                                            <  2:2056492/150‑3 (MQ=255)
               ggtggtGGCATCGGTGCTGGAGTTGATCAGTCTGTTGCATAGTCGTCCGTCAACGTGACTCTGGTTGGCTTTGGTAACTACTCGACCGCGCATCTGTTCTATTACTTCATTTGTTTTACAGAATCAGGGCGCAAGCCCTG‑‑TTTTTTTTCg                                                                                                                                       <  1:317774/150‑1 (MQ=255)
                gtggtgGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCgg                                                                                                                                      <  2:2028949/150‑1 (MQ=255)
                gtggtgGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCgg                                                                                                                                      <  2:1480075/150‑1 (MQ=255)
                gtggtgGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTAgg                                                                                                                                      >  2:546139/1‑150 (MQ=255)
                 ttgtggCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCTTCAACGTTACTCATGTTTGCTTTGGTAACAACTCTACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCggg                                                                                                                                     <  2:270215/148‑1 (MQ=255)
                 tggtggCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCggg                                                                                                                                     <  1:1550963/150‑1 (MQ=255)
                 tggtggCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCggg                                                                                                                                     <  2:528423/150‑1 (MQ=255)
                 tggtggCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCggg                                                                                                                                     <  2:1212723/150‑1 (MQ=255)
                 tggtggCAACGGTGATGAATTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGAATCAGGTTGGCTTTGGTAACAACGCGACCGCTCTTCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCggg                                                                                                                                     <  2:787350/150‑1 (MQ=255)
                 ggggggCAACGGTGCTGCAGTTGACCAGACGGCATCTAACTCCTCCGTCAACGTGACTCAGGGTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCggg                                                                                                                                     <  2:1522377/146‑1 (MQ=255)
                  ggtggCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGga                                                                                                                                    <  2:403827/150‑1 (MQ=255)
                     ggCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCCTCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCGCTG‑‑TGTTTTTTCGGGagaa                                                                                                                                 >  2:1035534/1‑150 (MQ=255)
                               ctgcAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAAt                                                                                                                       >  1:1103549/1‑150 (MQ=255)
                                 gcAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGAAACAACGCGACCGCTCATCAGTACTAATACATCATGTGTATTACAGAAACAGGGCTCAAGCCCTG‑‑TTTGTATACTCGAGAACAATATGACTaa                                                                                                                     >  2:959319/1‑149 (MQ=255)
                                  cAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTAGGGAGAAGAATATGAATACg                                                                                                                    >  1:1253061/1‑150 (MQ=255)
                                  cAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATATGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACg                                                                                                                    <  1:856139/150‑1 (MQ=255)
                                    gTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGtt                                                                                                                  >  2:887855/1‑150 (MQ=255)
                                     ttGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAAAACGtta                                                                                                                 >  2:1950872/1‑150 (MQ=255)
                                       gACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGttatt                                                                                                               >  2:628484/1‑150 (MQ=255)
                                       gACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGttatt                                                                                                               <  1:1318136/150‑1 (MQ=255)
                                       gACCAGACTGCATCTAACTCCTCCGTCAAAGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCATG‑‑TTTTTTTTCGTGAGAAGAATATGAATACGttatt                                                                                                               >  1:916544/1‑150 (MQ=255)
                                        aCCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGttatta                                                                                                              >  1:1876423/1‑150 (MQ=255)
                                             aCTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCt                                                                                                         >  1:1583832/1‑150 (MQ=255)
                                                gCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGc                                                                                                      >  1:2020111/1‑150 (MQ=255)
                                                gCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGc                                                                                                      >  1:1646460/1‑150 (MQ=255)
                                                 cATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCg                                                                                                     <  2:2103277/150‑1 (MQ=255)
                                                  aTCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCgg                                                                                                    <  2:1726829/150‑1 (MQ=255)
                                                   tCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGc                                                                                                   <  2:1289460/150‑1 (MQ=255)
                                                   tCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGc                                                                                                   <  2:1064917/150‑1 (MQ=255)
                                                    cTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCa                                                                                                  >  2:540143/1‑150 (MQ=255)
                                                     tgaCTCCTCCGTCAACGTGACTCAGGTTGGCTATGGTAACAACGCGACCGCTCATCAGTACTAATACATAATTTGTATTACAGAAACAGGGCTCAAGCCCTG‑‑TTATTTTTAGGGATCAGAATATGAATACGTTATTACTCATTGCGGCAc                                                                                                 >  1:129128/3‑150 (MQ=255)
                                                     tAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCAc                                                                                                 <  1:79566/150‑1 (MQ=255)
                                                     tAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCAc                                                                                                 >  1:881316/1‑150 (MQ=255)
                                                      aaCTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATAAGTTATTACTCCTTGCGGCACt                                                                                                >  2:2181054/1‑150 (MQ=255)
                                                        ctcctcCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAATCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACttt                                                                                              >  2:1609339/1‑150 (MQ=255)
                                                        ctcctcCGTCAACGTGACTCAGGTTGGCTTTCGTAACAAGGCGACCGCTCATCAGTACTTATACATCATTTGTATTACAGAAACAGGGCGCAAGACCTG‑‑TTTTTTTTCGTGAGAACAATATGAAGACGTTATTACACCTTGCgcccattt                                                                                              >  2:1131922/1‑143 (MQ=255)
                                                         acctccGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTc                                                                                             <  2:125741/149‑1 (MQ=255)
                                                          cctccGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTcc                                                                                            <  2:514055/150‑1 (MQ=255)
                                                            accGTCCACGGGACCCCGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGACACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAg                                                                                          <  1:969687/149‑1 (MQ=255)
                                                             ccGTCAACGTGACTCAGGTTGGCTTTGGAAACAACGCGACCGCTCATCAGTACAAATACATCATTTGTATAACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGt                                                                                         <  1:825375/150‑1 (MQ=255)
                                                              cGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCAAGTc                                                                                        >  2:2010135/1‑150 (MQ=255)
                                                                tCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAg                                                                                      <  2:1764132/150‑1 (MQ=255)
                                                                    cGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATaa                                                                                  <  1:1923366/150‑1 (MQ=255)
                                                                    cGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTAAGATaa                                                                                  >  1:1790644/1‑150 (MQ=255)
                                                                       gACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCt                                                                               >  2:671828/1‑150 (MQ=255)
                                                                         cTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCttt                                                                             <  1:1064438/150‑1 (MQ=255)
                                                                          gcAGGTGGGCTTTGGTTACATCGCGACCGCTCTTCAGTACAAATACAACATTTGTATTACAGTAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGTGAGAAGAATATGAATTCGTTATTACTCCTTGCGGCACTTTCGAGTCAGATAACCTTTa                                                                            <  1:212348/149‑1 (MQ=255)
                                                                           cAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTaa                                                                           <  2:198347/150‑1 (MQ=255)
                                                                           cAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAAAACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTaa                                                                           >  1:1745557/1‑150 (MQ=255)
                                                                           cAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCATG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTaa                                                                           >  2:1409373/1‑150 (MQ=255)
                                                                            tggTTGGCTTTGTTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACATGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAAt                                                                          <  1:725032/149‑1 (MQ=255)
                                                                             ggTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATa                                                                         <  2:939036/150‑1 (MQ=255)
                                                                               ttGGCTTTGGGAACCACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACACGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACg                                                                       <  2:221633/150‑1 (MQ=255)
                                                                                    tttGGTAACAACGCGACCGCTCATCAGTACTAATACCTCAGTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGTTAGAATAATATGTCTACGTTATTACTCCGTGCGGCACTTTCCAGTCAGATAACCTTTAAGACGAcccc                                                                  >  2:1581377/1‑149 (MQ=255)
                                                                                     ttGGGAACAACTCGACCGGTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCGCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAg                                                                 <  2:167584/150‑1 (MQ=255)
                                                                                      tGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGc                                                                <  2:1671639/150‑1 (MQ=255)
                                                                                        gTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCaa                                                              >  1:1983040/1‑150 (MQ=255)
                                                                                        gTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACACAGCaa                                                              >  1:1506959/1‑150 (MQ=255)
                                                                                           acaacGCGTACTCTCATCATTACTAATACTTCATTTGTATTACAGAAACAGGGCGCAAGCCCTGTTTTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAg                                                             <  2:180072/150‑1 (MQ=255)
                                                                                           acaacGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAggg                                                           >  1:983654/1‑150 (MQ=255)
                                                                                             aacGCGACCGCTCATCAGTACTAATAAATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGa                                                         >  1:1399400/1‑150 (MQ=255)
                                                                                               cgcgACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGAtg                                                       <  2:968958/150‑1 (MQ=255)
                                                                                                    ccGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGtat                                                  >  2:1774670/1‑150 (MQ=255)
                                                                                                    ccGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGtat                                                  >  2:1765784/1‑150 (MQ=255)
                                                                                                           tcaGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCatta                                           <  1:2154218/150‑1 (MQ=255)
                                                                                                            caGTACTAATACATCATATGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCattat                                          <  1:463073/150‑1 (MQ=255)
                                                                                                                aCTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCt                                      >  1:2010340/1‑150 (MQ=255)
                                                                                                                 cTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTg                                     >  1:514791/1‑150 (MQ=255)
                                                                                                                   aaTACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACACAGCAAGGGGATGTGTATACGATTATTCCTGaa                                   >  2:1324624/1‑150 (MQ=255)
                                                                                                                    aTACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAg                                  <  1:800818/150‑1 (MQ=255)
                                                                                                                     tACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGt                                 >  1:1606730/1‑150 (MQ=255)
                                                                                                                        atcatTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCAc                              <  1:1817484/150‑1 (MQ=255)
                                                                                                                        atcatTTGTATTACAGAAACAGGGCGAAAGCCCTT‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCATTCAGATAACCTTTTATACGACCCAGCCAGGGGATGTGTATACCATTATTCCTGAAGTCAc                              <  2:1487815/150‑1 (MQ=255)
                                                                                                                         tcatTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATATCCTTTAATACGACCCATCAAGGGGATGTGTATAACATTATTCCTGAAGTCAct                             >  2:94092/1‑150 (MQ=255)
                                                                                                                                tATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACta                      >  2:1806314/1‑149 (MQ=255)
                                                                                                                                 cgaaaaaaaaCAGGGCTTGCGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTGTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCa                     >  2:933326/8‑150 (MQ=255)
                                                                                                                                 aTTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCa                     >  1:628017/1‑150 (MQ=255)
                                                                                                                                 aTTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCa                     >  1:1559545/1‑150 (MQ=255)
                                                                                                                                  ttACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCaa                    >  1:2136941/1‑150 (MQ=255)
                                                                                                                                    aCAGAAACAGGGCTAAAGCCATG‑‑TTTTTTTTCGGGAGAAGAATCTGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCAAGCAAGGGGTTGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATc                  <  1:959319/150‑1 (MQ=255)
                                                                                                                                     cAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATAAGAATACGTTATTACTCCTTGCGGCACTTTCAAGTCAGATAACCTTTAATAATACCAAGCAAGGGGATGTGTATACCAGTATTACTGAAGTCACTCTTACTCAATCt                 >  2:1060908/1‑150 (MQ=255)
                                                                                                                                       gAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTg               >  2:625290/1‑150 (MQ=255)
                                                                                                                                        aaaCAGGTCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGTCACTTTCCAGTCAGATAACCTTTAATTCGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGt              <  1:1950872/150‑1 (MQ=255)
                                                                                                                                         aaCATGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGGCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACAATTATTCCTGAAGTCACTCTTACTCAATCTTGTc             <  2:1885938/150‑1 (MQ=255)
                                                                                                                                         aaCACGGCGCAAGCCCTT‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTAGTACTCCTTGCGGCACTTTCCAGTCAGATACCCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTc             <  2:350149/150‑1 (MQ=255)
                                                                                                                                          aCAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCt            <  2:881316/150‑1 (MQ=255)
                                                                                                                                           cAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCtg           >  1:631141/1‑150 (MQ=255)
                                                                                                                                              ggCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGc        >  1:2073854/1‑150 (MQ=255)
                                                                                                                                                 gcAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTACACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCaga     >  1:1859105/1‑150 (MQ=255)
                                                                                                                                                  cAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCagag    <  2:2020111/150‑1 (MQ=255)
                                                                                                                                                    aGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCAGAGTa  <  1:1409373/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                       
ACGGTTAAACAGTTCGGTGGTGGCAACGGTGCTGCAGTTGACCAGACTGCATCTAACTCCTCCGTCAACGTGACTCAGGTTGGCTTTGGTAACAACGCGACCGCTCATCAGTACTAATACATCATTTGTATTACAGAAACAGGGCGCAAGCCCTG‑‑TTTTTTTTCGGGAGAAGAATATGAATACGTTATTACTCCTTGCGGCACTTTCCAGTCAGATAACCTTTAATACGACCCAGCAAGGGGATGTGTATACCATTATTCCTGAAGTCACTCTTACTCAATCTTGTCTGTGCAGAGTA  >  NC_000913/1104786‑1105083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: