Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,129,394 G→C 5.4% intergenic (+20/+20) murJ → / ← flgN putative lipid II flippase/export chaperone for FlgK and FlgL

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,129,3940GC 5.4% 340.1 / 8.2 108intergenic (+20/+20)murJ/flgNputative lipid II flippase/export chaperone for FlgK and FlgL
Reads supporting (aligned to +/- strand):  ref base G (55/47);  new base C (4/2);  total (59/49)
Fisher's exact test for biased strand distribution p-value = 6.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 124 total aligned reads displayed.

GTCATTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGCCTGTTGATTGCGCTCAATCTGTCCTTCCAGT  >  NC_000913/1129245‑1129543
                                                                                                                                                     |                                                                                                                                                     
ggcaatGGGTACCATGCGCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTTCTTCGCTGCACTGGCGGTACTTGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCtgcag                                                                                                                                                       <  2:1908467/145‑1 (MQ=255)
 tCATTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTTGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCtgcagt                                                                                                                                                      <  2:1496600/150‑1 (MQ=255)
  cATTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCTGTCGTGAGGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAATTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCtgcagtg                                                                                                                                                     <  1:875947/150‑1 (MQ=255)
  cATTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCtgcagtg                                                                                                                                                     >  1:976236/1‑150 (MQ=255)
  cATTGGGTACCATGCCCTGGCGTTTACTGCGTTTAAAGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCtgcagtg                                                                                                                                                     <  2:870207/150‑1 (MQ=255)
  cATTGGGTACCATGCCCTGGCGTTTACTGCGTATAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTCGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCCGACCGTGTATCAATGCATTCCCGCCtgcagtg                                                                                                                                                     >  1:606726/1‑150 (MQ=255)
     tGGGTACCATGCCCTGGCGTTTACTGCGTTTACTGGCGGGCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACGGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCtgcagtgcag                                                                                                                                                  <  2:2155582/150‑1 (MQ=255)
      gggTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAgg                                                                                                                                                 >  2:1764422/1‑150 (MQ=255)
       ggTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGc                                                                                                                                                >  1:1996333/1‑150 (MQ=255)
          aCCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCgg                                                                                                                                             <  1:492308/150‑1 (MQ=255)
             aTGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGgaga                                                                                                                                          >  1:2001629/1‑150 (MQ=255)
              tGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGAt                                                                                                                                         <  2:1227048/150‑1 (MQ=255)
               gCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATa                                                                                                                                        >  2:1567809/1‑150 (MQ=255)
                  cTGGCGTCTACTGCGGTTAATGGCGGGCGGGCTGGCGGGGATTGCCGCGTACTTCGCTGCACGGGCGGTACTGGGCTTCAAAGTTAAAGAATATGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATc                                                                                                                                     <  1:1607557/150‑1 (MQ=255)
                     gCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTc                                                                                                                                  <  2:1767022/150‑1 (MQ=255)
                     gCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTc                                                                                                                                  <  1:1169782/150‑1 (MQ=255)
                      cGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCa                                                                                                                                 >  2:185287/1‑150 (MQ=255)
                          tACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCTCGGGCGGTACTGGGCTGCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACACTGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATc                                                                                                                             <  1:1977224/150‑1 (MQ=255)
                           aCTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGAGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCg                                                                                                                            >  2:255963/1‑150 (MQ=255)
                             tGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGaa                                                                                                                          >  1:1048149/1‑150 (MQ=255)
                             tGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGaa                                                                                                                          >  2:2173083/1‑150 (MQ=255)
                                gTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGTCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATc                                                                                                                       >  1:155297/1‑150 (MQ=255)
                                gTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATc                                                                                                                       >  2:1416299/1‑150 (MQ=255)
                                 tttCATGGCGGGCGCGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGTTAATCTTCAGATCGAAATCt                                                                                                                      <  2:1097690/150‑1 (MQ=255)
                                 ttaaaTGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGGACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGTTAATCTTCAGATCGAAATCt                                                                                                                      <  2:1104818/147‑1 (MQ=255)
                                   tAATGTCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCAGTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCttt                                                                                                                    <  2:1344357/150‑1 (MQ=255)
                                   tAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCttt                                                                                                                    >  1:11399/1‑150 (MQ=255)
                                   tAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCttt                                                                                                                    >  2:1798833/1‑150 (MQ=255)
                                    aaTGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCtttt                                                                                                                   <  2:625405/150‑1 (MQ=255)
                                     atcgCGGACGCGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCttttt                                                                                                                  <  2:284878/147‑1 (MQ=255)
                                     aTGGCGGTCGGGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGAGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTTCAGGCCGGAGATAATCTTCAGATCGAAATCttttt                                                                                                                  <  1:1921051/150‑1 (MQ=255)
                                      tGGCGGTCGTGCTGGCGGGGATTGCCGCGAACTTCGCTGCACTGGCGGTACTGGGATTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCCGGCCGGAGATAATCTTGAGATCGAAATCTTTTTa                                                                                                                 >  1:277838/1‑150 (MQ=255)
                                        gCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTAcc                                                                                                               >  1:1669739/1‑150 (MQ=255)
                                            tCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTAccgccg                                                                                                           <  2:2027872/150‑1 (MQ=255)
                                               tGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGa                                                                                                        >  1:539775/1‑150 (MQ=255)
                                                 cTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATg                                                                                                      <  2:653661/150‑1 (MQ=255)
                                                  tGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCttttt                                                                                                                  <  2:1114787/137‑1 (MQ=255)
                                                  tGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCttttt                                                                                                                  >  1:1114787/1‑137 (MQ=255)
                                                  tGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATgg                                                                                                     <  1:1311725/150‑1 (MQ=255)
                                                   ggCGGGGATTGCCGCGTACATCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATggg                                                                                                    <  1:1764992/150‑1 (MQ=255)
                                                        ggATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCCGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTAACGCCGCGATGGgttgtt                                                                                               >  2:1612995/1‑150 (MQ=255)
                                                         gATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGttgttg                                                                                              <  1:876394/150‑1 (MQ=255)
                                                           ttGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAg                                                                                            <  1:691141/150‑1 (MQ=255)
                                                             gCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGt                                                                                          >  1:973389/1‑150 (MQ=255)
                                                              ccGCGTACTTCGCTGCAATTGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACCATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTc                                                                                         >  1:1234404/1‑150 (MQ=255)
                                                                gcgTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTg                                                                                       >  1:48997/1‑150 (MQ=255)
                                                                   tACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGAcc                                                                                    >  2:1481535/1‑150 (MQ=255)
                                                                     cTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGt                                                                                  >  1:783383/1‑150 (MQ=255)
                                                                     cTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGt                                                                                  >  1:1887672/1‑150 (MQ=255)
                                                                     cTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGt                                                                                  >  1:2005299/1‑150 (MQ=255)
                                                                     cTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAAAGCATTCCGTCATGAAGTTCAGGCCGGAGATTATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTTTTTAGGTATGACCGt                                                                                  >  2:1964557/1‑150 (MQ=255)
                                                                       tCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGtta                                                                                >  1:1724206/1‑149 (MQ=255)
                                                                         gCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCCGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTTCCCGTTCGc                                                                              >  1:1971653/1‑150 (MQ=255)
                                                                          cTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGcc                                                                             <  2:1028596/150‑1 (MQ=255)
                                                                                 ggCGGTACTGGGCTTCAAAGTTAAAGAATTTGACCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGTACGAAATCTTTTTACCTCGGCGATGCGATTATGAGGACTTAAAGTTAGACCCAtata                                                                      >  1:397242/1‑150 (MQ=255)
                                                                                 ggCGGTACTGGGCTTCAAAGTTAAAGAATCTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCAtata                                                                      >  1:762886/1‑150 (MQ=255)
                                                                                          gggCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGtt                                                             >  1:1604334/1‑150 (MQ=255)
                                                                                          gggCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGAGGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGtt                                                             >  1:488688/1‑150 (MQ=255)
                                                                                            gCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGGCTGCAGTGCAGGCCGGAGAATATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGGGTCGGTTcc                                                           >  2:222328/1‑150 (MQ=255)
                                                                                                 aaaGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATg                                                      >  2:2154448/1‑150 (MQ=255)
                                                                                                   aGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCg                                                    >  1:1363773/1‑150 (MQ=255)
                                                                                                       aaaGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGttt                                                >  2:1032373/1‑150 (MQ=255)
                                                                                                       aaaGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCAGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGttt                                                >  1:2159167/1‑150 (MQ=255)
                                                                                                        aaGAATTTGCCCGCCGGCCCGTGTAACAATGCCTTCCGGTCTGCACTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGCTGGGTTGTGGAGGGCTGACCGGTCGCCCCCTATAGCGTCGGTTCCTGATGCGGTTTc                                               <  2:155297/150‑1 (MQ=255)
                                                                                                         aGAATTTGCCCGCCGGACTGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCGGGATATAATCTTCAGATCGAAATCTTTTTACCGCCGCTATGGGTTGTTGAGGTCTGACCGTTCTCCCCATATAGCGTCGGTTCCTGATGCGGTTTCa                                              <  1:1567809/150‑1 (MQ=255)
                                                                                                         aGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCa                                              >  2:2106525/1‑150 (MQ=255)
                                                                                                          caaTATGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCaa                                             <  2:759076/149‑1 (MQ=255)
                                                                                                           aaTTTGCCCGCCGGTCGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGACGGAGATAATCTTCAGATTGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTTATGCGGTTTCAAc                                            <  1:1471544/150‑1 (MQ=255)
                                                                                                           aaTTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGTTAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAAc                                            <  1:1416299/150‑1 (MQ=255)
                                                                                                               tGCCCGCCGGACGGTGTAACAATTTCTTCCTGCGTGCAGGGCAGGCCGGAGATAATCTTCAGATCGAAATAGATTTACCGCCGCGATGGGTTGTTGATGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACAttt                                        <  2:1936875/150‑1 (MQ=255)
                                                                                                                 cccGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTcc                                      <  2:1669739/150‑1 (MQ=255)
                                                                                                                  ccgccgGCAGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATATTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCa                                     <  2:1132127/150‑1 (MQ=255)
                                                                                                                  ccgccgGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCa                                     >  2:401460/1‑150 (MQ=255)
                                                                                                                   cgccgGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGCCCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAg                                    >  1:1117210/1‑150 (MQ=255)
                                                                                                                     ccgGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGCTGCGGTTTCAACATTTCCAgcg                                  >  2:842111/1‑150 (MQ=255)
                                                                                                                      cgGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGcc                                <  1:2173083/150‑1 (MQ=255)
                                                                                                                           ggTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGCCTGtt                            <  1:1764422/150‑1 (MQ=255)
                                                                                                                            gtgtAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGCCTGTTg                           <  2:1648257/150‑1 (MQ=255)
                                                                                                                            gtgtAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGCCTGTTg                           >  1:1644085/1‑150 (MQ=255)
                                                                                                                               tAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGCCTGTTGAtt                        >  2:1028876/1‑150 (MQ=255)
                                                                                                                                aaCAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGCCTGTTGATTg                       <  2:2154873/150‑1 (MQ=255)
                                                                                                                                aaCAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGCCTGTTGATTg                       >  2:1435878/1‑150 (MQ=255)
                                                                                                                                   aaTGCATTGCGGCCTGCAGTGCAGGCCGGTGATAATCTTCAGATCGAATTCTTTTTACCGCCGGGATGGGGTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTAGAGGGCGTGTTGATTgggg                    <  2:2033185/150‑4 (MQ=255)
                                                                                                                                    aTGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGCCTGTTGATTGCGCt                   <  1:185287/150‑1 (MQ=255)
                                                                                                                                    aTGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGCCTGTTGATTGCGCt                   >  2:16647/1‑150 (MQ=255)
                                                                                                                                      tcATTCAGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGTTGGAAATCTTTTTACCGCCTCGATGTGTTGTTGAGGTCTGACCGTTCGCCCCTTATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGCCTGTTGATTGCGCTCa                 <  2:1971653/149‑1 (MQ=255)
                                                                                                                                       cATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGCGTGTTGATTGCGCTCaa                >  1:826899/1‑150 (MQ=255)
                                                                                                                                         ttCCGGCCTGCAGTGCAGGCCGGATATAATCTTCAGATAGAAATCTTTTTCCCGCCGGGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGCCTGTTGATTGCGCTCAATc              <  1:1817984/150‑1 (MQ=255)
                                                                                                                                         ttCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGTGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGCCTGTTGATTGCGCTCAATc              <  1:982656/150‑1 (MQ=255)
                                                                                                                                         ttCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGCCTGTTGATTGCGCTCACTc              >  1:1479440/1‑150 (MQ=255)
                                                                                                                                         ttCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGCCTGTTGATTGCGCTCAATc              <  2:1887672/150‑1 (MQ=255)
                                                                                                                                          tCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGCCTGTTGATTGCGCTCAATCt             <  2:539775/150‑1 (MQ=255)
                                                                                                                                          tCCGGCCTGCACTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGCCTGTTGATTGCGCTCAATCt             >  1:947140/1‑150 (MQ=255)
                                                                                                                                           ccGGCCTGCACTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTTAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGCCTGTTGATTGCGCTCAATCTg            >  2:1045424/1‑150 (MQ=255)
                                                                                                                                           ccGGCCTGCACTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGCCTGTTGATTGCGCTCAATCTg            >  1:1313745/1‑150 (MQ=255)
                                                                                                                                           ccGGCCTGCACTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGGTTCAACATTTCCAGCGCCTGTTGATTGCGCTCAATCTg            >  2:1071197/1‑150 (MQ=255)
                                                                                                                                            cGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGCCTGTTGATTGCGCTCAATCTGt           <  2:1996333/150‑1 (MQ=255)
                                                                                                                                                cTGCAGGGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACGGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGCCTGTTGATTGCGCTCAATCTGTCCtt       <  1:211579/150‑1 (MQ=255)
                                                                                                                                                  gcagtgcagGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGCCTGTTGATTGCGCTCAATCTGTCCTTcc     <  2:2005299/150‑1 (MQ=255)
                                                                                                                                                  gcagtgcagGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGCCTGTTGATTGCGCTCAATCTGTCCTTcc     <  2:973020/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTCATTGGGTACCATGCCCTGGCGTTTACTGCGTTTAATGGCGGTCGTGCTGGCGGGGATTGCCGCGTACTTCGCTGCACTGGCGGTACTGGGCTTCAAAGTTAAAGAATTTGCCCGCCGGACGGTGTAACAATGCATTCCGGCCTGCAGTGCAGGCCGGAGATAATCTTCAGATCGAAATCTTTTTACCGCCGCGATGGGTTGTTGAGGTCTGACCGTTCGCCCCATATAGCGTCGGTTCCTGATGCGGTTTCAACATTTCCAGCGCCTGTTGATTGCGCTCAATCTGTCCTTCCAGT  >  NC_000913/1129245‑1129543

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: