Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,232,481 C→T 6.8% intergenic (+27/+19) umuC → / ← dsbB translesion error‑prone DNA polymerase V subunit; DNA polymerase activity/oxidoreductase that catalyzes reoxidation of DsbA protein disulfide isomerase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,232,4810CT 6.8% 316.5 / 8.4 102intergenic (+27/+19)umuC/dsbBtranslesion error‑prone DNA polymerase V subunit; DNA polymerase activity/oxidoreductase that catalyzes reoxidation of DsbA protein disulfide isomerase I
Reads supporting (aligned to +/- strand):  ref base C (50/45);  new base T (5/2);  total (55/47)
Fisher's exact test for biased strand distribution p-value = 4.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.83e-01

Only 100 of 104 total aligned reads displayed.

AGAGGGCAGAGGAACACTCTATTTTGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCA  >  NC_000913/1232334‑1232628
                                                                                                                                                   |                                                                                                                                                    
agagGGCAGAGGAACACTCTATTTTGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGATAAACGCGCTCCCGc                                                                                                                                                    >  2:101541/1‑150 (MQ=255)
 gagGGCAGAGGAACACTCTATTTTGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCa                                                                                                                                                   <  2:1866063/150‑1 (MQ=255)
   gggCAGAGGAACACTCTATTTTGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAgg                                                                                                                                                 >  2:758887/1‑150 (MQ=255)
     gCAGAGGAACACTCTATTTTGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAg                                                                                                                                               >  1:1204921/1‑150 (MQ=255)
      cAGAGGAACACTCTATTTTGCCGGGCAGGGGATCCAGCAACAAGGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAACCGCGCTCCTGCGGGAgc                                                                                                                                              <  1:318999/150‑1 (MQ=255)
         agGAACACTCTATTTTGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAATCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGcc                                                                                                                                           <  1:334158/150‑1 (MQ=255)
          ggAACACTCTATTTTGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCg                                                                                                                                          <  2:969598/150‑1 (MQ=255)
           gAACACTCTATTTTGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCTGCGGGAgcgcttt                                                                                                                                         <  1:990377/150‑4 (MQ=255)
             acacTCTATTTTGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAAt                                                                                                                                       <  2:513668/150‑1 (MQ=255)
             acacTCTATTTTGCCGGGCAGGGGATCCAGAAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAAt                                                                                                                                       <  1:1932452/150‑1 (MQ=255)
                ctctATTTTGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCCGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGa                                                                                                                                    <  1:417244/150‑1 (MQ=255)
                 tctATTTTGCCTGGCAGGGTATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTAAACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGAt                                                                                                                                   <  1:188407/150‑1 (MQ=255)
                      tttGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCg                                                                                                                              <  2:534697/150‑1 (MQ=255)
                        tGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGAc                                                                                                                            >  2:413883/1‑150 (MQ=255)
                         gCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGAcc                                                                                                                           >  1:670607/1‑150 (MQ=255)
                           cGGGCAGGGGATCCAGCAACATTGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGa                                                                                                                         <  1:439675/150‑1 (MQ=255)
                           cGGGCAGGGGATCCAGCAACAATGGCCGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGa                                                                                                                         <  1:272059/150‑1 (MQ=255)
                               cAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAg                                                                                                                     >  1:1552619/1‑150 (MQ=255)
                                 ggggATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGAt                                                                                                                   >  2:2070892/1‑150 (MQ=255)
                                   ggATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGCCCGAACAGATcc                                                                                                                 >  1:1011981/1‑149 (MQ=255)
                                   ggATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCa                                                                                                                 >  1:248984/1‑150 (MQ=255)
                                      tCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGt                                                                                                              >  1:1827074/1‑150 (MQ=255)
                                       ccAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAACGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGtt                                                                                                             >  1:91980/1‑150 (MQ=255)
                                         aGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGtttt                                                                                                           >  1:1014717/1‑150 (MQ=255)
                                          gCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGttttt                                                                                                          <  2:126590/150‑1 (MQ=255)
                                           caacaaTGGCAGATGAAGCGAGCCATGCTATCACCACCTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAACTAAATATAGCGGAAGGAAAAAAGCGCTACCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGtttttt                                                                                                         <  1:137621/150‑1 (MQ=255)
                                            aacaaTGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTc                                                                                                        <  1:1521417/150‑1 (MQ=255)
                                            aacaaTGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTc                                                                                                        >  2:858431/1‑150 (MQ=255)
                                            aacaaTCGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGACGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTc                                                                                                        <  2:1737758/150‑1 (MQ=255)
                                              caaTGGTAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGGTTTTTCGc                                                                                                      >  2:600602/1‑150 (MQ=255)
                                              caaTGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGc                                                                                                      >  1:49314/1‑150 (MQ=255)
                                               aaTGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCt                                                                                                     >  1:82755/1‑150 (MQ=255)
                                               aaTGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCt                                                                                                     <  1:157749/150‑1 (MQ=255)
                                                aTGGCAGATGAATCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCtt                                                                                                    <  2:1196758/150‑1 (MQ=255)
                                                aTGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCtt                                                                                                    <  1:1298100/150‑1 (MQ=255)
                                                aTGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCtt                                                                                                    >  2:1672259/1‑150 (MQ=255)
                                                     aGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAAc                                                                                               <  1:1760612/150‑1 (MQ=255)
                                                      gATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACg                                                                                              <  1:2016812/150‑1 (MQ=255)
                                                         gAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATACCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTGGGTTTAACCGGCt                                                                                           >  2:207519/1‑150 (MQ=255)
                                                          aaGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTg                                                                                          >  1:280011/1‑150 (MQ=255)
                                                            gCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGCTCACGTTTTTTCGCTTTAAACGGCTggg                                                                                        >  1:771138/1‑150 (MQ=255)
                                                                 ccATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATc                                                                                   <  2:948957/150‑1 (MQ=255)
                                                                  cATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATca                                                                                  <  2:1552619/150‑1 (MQ=255)
                                                                       tttCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCaccacc                                                                             <  1:1883765/150‑1 (MQ=255)
                                                                          caccacGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGc                                                                          <  2:2048560/150‑1 (MQ=255)
                                                                            ccacGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCag                                                                        <  1:1281220/150‑2 (MQ=255)
                                                                            ccacGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCAc                                                                        >  2:1575614/1‑150 (MQ=255)
                                                                            ccaaGTTATACAACGCGAAGTTCTGATTTACTGAGTGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCTGGAGCGCCGAATGGAGTAGCGACCGAACAGATCCCGTGTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCAc                                                                        <  1:1047295/150‑1 (MQ=255)
                                                                               ggTTATACAACGCGAAGTTCTGATTTTCTGAGGTTCTAATAAATATTGCGGCAGGAAAATAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGATTTTTCGCTTTAAACGGCTGGGAAATCACCTCCAGCACTGc                                                                    <  2:967099/149‑1 (MQ=255)
                                                                                gTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCCACCGAACAGATCACGTATTTTCGCTTTAAACGCCTCGGAAATCACCACCACCACTGCg                                                                   >  2:424969/1‑150 (MQ=255)
                                                                                 ttATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGa                                                                  >  2:106371/1‑150 (MQ=255)
                                                                                  tataCAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGAc                                                                 <  1:1313443/150‑1 (MQ=255)
                                                                                   ataCAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAACGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGATGTAAACCGGGGGGAAATAACCACCAGCAATGCGACa                                                                >  2:376817/1‑150 (MQ=255)
                                                                                    taCAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAACAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACaa                                                               >  1:1108093/1‑150 (MQ=255)
                                                                                    taCAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACaa                                                               >  1:1299425/1‑150 (MQ=255)
                                                                                     aCAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAAt                                                              >  1:739011/1‑150 (MQ=255)
                                                                                      cAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCTCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAACCGGCTGGGAAATCACCACCAGCACTGCTACAAta                                                             >  2:1374638/1‑149 (MQ=255)
                                                                                      cAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATc                                                             >  1:1733072/1‑150 (MQ=255)
                                                                                      cAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATc                                                             >  2:1364919/1‑150 (MQ=255)
                                                                                      cAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCAAGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATc                                                             >  1:202779/1‑150 (MQ=255)
                                                                                       aaCGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCa                                                            <  2:670607/150‑1 (MQ=255)
                                                                                       aaCGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCa                                                            >  2:1175176/1‑150 (MQ=255)
                                                                                         cgcgAAGTTCTGATTTACTGAGGGTCAAATTATTATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGTAATTCACATCATCACGTGCGACAATAAgt                                                          >  2:231785/1‑149 (MQ=255)
                                                                                          gcgAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGt                                                         <  1:37900/150‑1 (MQ=255)
                                                                                           cgAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTa                                                        >  1:1595973/1‑150 (MQ=255)
                                                                                             aaGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAg                                                      >  2:1462281/1‑150 (MQ=255)
                                                                                                 tCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGAt                                                  >  1:650114/1‑150 (MQ=255)
                                                                                                    gATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATaaa                                               >  2:277372/1‑150 (MQ=255)
                                                                                                          cTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGCGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATAcc                                         >  1:1384386/1‑150 (MQ=255)
                                                                                                           tGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCg                                        <  1:1582840/150‑1 (MQ=255)
                                                                                                           agAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGAGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACGACCAGCACTGCGACAATCAGGTACGCGATAAAAATACCg                                        >  1:91227/2‑150 (MQ=255)
                                                                                                            gtgggTCAAATAAATATAGCGGCAGGAAAAAAGCGCTGCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGa                                       <  1:718818/148‑1 (MQ=255)
                                                                                                             aGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGag                                      <  2:1013153/150‑1 (MQ=255)
                                                                                                               ggTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGagca                                    >  2:559440/1‑150 (MQ=255)
                                                                                                               ggTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGagca                                    <  1:1982337/150‑1 (MQ=255)
                                                                                                                  cAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGcc                                 <  1:288116/150‑1 (MQ=255)
                                                                                                                  cAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGcc                                 <  1:2095567/150‑1 (MQ=255)
                                                                                                                    aataaataTAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCAc                               >  2:89522/1‑150 (MQ=255)
                                                                                                                    aataaataTAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCAc                               >  1:455154/1‑150 (MQ=255)
                                                                                                                    aataaataTAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCAc                               >  2:1638143/1‑150 (MQ=255)
                                                                                                                          tataGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGc                         >  1:1680491/1‑150 (MQ=255)
                                                                                                                            taGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGCCCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCCGCAt                       >  1:1762936/1‑150 (MQ=255)
                                                                                                                               cGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTc                    <  1:1427018/150‑1 (MQ=255)
                                                                                                                                ggCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTcc                   <  2:1827074/150‑1 (MQ=255)
                                                                                                                                ggCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTcc                   <  1:1462281/150‑1 (MQ=255)
                                                                                                                                 gCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCa                  <  2:1204921/150‑1 (MQ=255)
                                                                                                                                  cAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAg                 >  2:1545224/1‑150 (MQ=255)
                                                                                                                                  cAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAg                 <  1:1175176/150‑1 (MQ=255)
                                                                                                                                  cAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAg                 >  1:1138017/1‑150 (MQ=255)
                                                                                                                                     gAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGAcc              <  1:1209445/150‑1 (MQ=255)
                                                                                                                                      cattcggcgcTCCTGCGGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCGTTTCCAGACCt             >  1:1502096/7‑150 (MQ=255)
                                                                                                                                      cattcggcgcTCCTGCGGGAGCGCCGAAGGGATTAGCGCCCGAACAGCTCCCGGTTTTTCGCGGTACACGGCTGGGAAATCACCCCCCGCACTGCGACAAGCAGGGAAGCGATAAAAATACCGAGCAGCCGCTGCGGCATTTCCAGAccg             >  1:1510721/7‑149 (MQ=255)
                                                                                                                                       attcggcgcTCCTGCGGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGAGAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTa            >  2:1758406/6‑150 (MQ=255)
                                                                                                                                         tcggcgcTCCTGCGGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTaaa          >  1:626131/4‑150 (MQ=255)
                                                                                                                                          cggcgcTCCTGCGGGAGCGCCGAATGGATTAGCGACCGAACAGAGCACGTTTTTTCGCGTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCCCTGCGGCAGTGCAAGCCCTaaaa         >  2:1448665/3‑150 (MQ=255)
                                                                                                                                             cgcTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCCCGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAAt      <  1:106371/150‑1 (MQ=255)
                                                                                                                                             cgcTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAAt      >  2:152650/1‑150 (MQ=255)
                                                                                                                                              gcTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATc     <  1:960764/150‑1 (MQ=255)
                                                                                                                                                 cccGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATATAAATCCCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAACTcaca  >  1:1088124/1‑150 (MQ=255)
                                                                                                                                                 cccGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATACCa  >  2:508520/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
AGAGGGCAGAGGAACACTCTATTTTGCCGGGCAGGGGATCCAGCAACAATGGCAGATGAAGCGAGCCATGCTTTCACCACGTTATACAACGCGAAGTTCTGATTTACTGAGGGTCAAATAAATATAGCGGCAGGAAAAAAGCGCTCCCGCAGGAGCGCCGAATGGATTAGCGACCGAACAGATCACGTTTTTTCGCTTTAAACGGCTGGGAAATCACCACCAGCACTGCGACAATCAGGTAAGCGATAAAAATACCGAGCAGCCACTGCGGCATTTCCAGACCTAAAAAATCCCA  >  NC_000913/1232334‑1232628

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: