Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,324,837 A→C 5.7% Y31S (TAT→TCT) ‡ yciQ → enhancer of membrane protein expression; putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,324,8370AC 5.7% 357.6 / 9.7 122Y31S (TAT→TCT) ‡yciQenhancer of membrane protein expression; putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (64/51);  new base C (5/2);  total (69/53)
Fisher's exact test for biased strand distribution p-value = 6.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.70e-01

Only 100 of 127 total aligned reads displayed.

AGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATGGATGCAGCCTG  >  NC_000913/1324688‑1324986
                                                                                                                                                     |                                                                                                                                                     
aGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTa                                                                                                                                                       <  2:2108353/150‑1 (MQ=255)
aGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTa                                                                                                                                                       <  1:1540822/150‑1 (MQ=255)
 ggCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTAt                                                                                                                                                      >  1:1964109/1‑150 (MQ=255)
   cGTTGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGTAGACTATTGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGAACCCTTCTTATga                                                                                                                                                    <  2:1122623/150‑1 (MQ=255)
    gTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGGTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATgaa                                                                                                                                                   <  2:765162/150‑1 (MQ=255)
       ggTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATgaagaa                                                                                                                                                <  1:1714018/150‑1 (MQ=255)
        gTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGaagaag                                                                                                                                               >  2:1236900/1‑150 (MQ=255)
          gATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAggg                                                                                                                                             <  2:877098/150‑1 (MQ=255)
           aTGTGAAGCCTTTCTTATACCAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGGTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGa                                                                                                                                            <  2:556518/150‑1 (MQ=255)
           aTGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAATTCTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGa                                                                                                                                            <  1:2103724/150‑1 (MQ=255)
            tgtgAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTATGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGaa                                                                                                                                           >  2:2136745/1‑149 (MQ=255)
             gtgACGCCTGTCTTATAACACATTGCGTTCCCCGGATGGCAGACTAAGGCAGGGAAGGTTCGCTGCATTTGGCTGGTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTGCATAAGAAGGGAtc                                                                                                                                          <  2:424275/150‑1 (MQ=255)
             gtgAAGCCTTTCTTATAACAAATTGCGTTCCACGTATGGAAGACTATGGCAGGGAAGTTTCGCTTCATTTTGATGTTTATAGCAGTGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACTGAGATCCCTTCTTATGAAGTATGGAta                                                                                                                                          >  1:370670/1‑149 (MQ=255)
              tgAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGAtct                                                                                                                                         >  2:842041/1‑150 (MQ=255)
                 aGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGc                                                                                                                                      <  2:1200031/150‑1 (MQ=255)
                   ccTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGctc                                                                                                                                    <  2:58425/150‑1 (MQ=255)
                    cTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGctct                                                                                                                                   >  2:2112006/1‑150 (MQ=255)
                      ttCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCttt                                                                                                                                 <  1:896893/150‑1 (MQ=255)
                       tCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTg                                                                                                                                >  1:188192/1‑150 (MQ=255)
                        cTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGa                                                                                                                               <  2:1339206/150‑1 (MQ=255)
                          tataACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATg                                                                                                                             >  2:1324206/1‑150 (MQ=255)
                          tataACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATg                                                                                                                             <  1:35336/150‑1 (MQ=255)
                           ttaACAAATTGCGTTCCACGGATGGAAGACTATGGGAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATAATCTCTAAGTTATGCAGAAAACACTGATATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGt                                                                                                                            <  2:598224/149‑1 (MQ=255)
                           ataACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTAGATGt                                                                                                                            >  1:2104379/1‑150 (MQ=255)
                            taACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGtt                                                                                                                           >  1:185645/1‑150 (MQ=255)
                            taACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGtt                                                                                                                           >  1:249779/1‑150 (MQ=255)
                             aaCAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTg                                                                                                                          >  2:1891121/1‑150 (MQ=255)
                               cAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGaa                                                                                                                        <  1:13910/150‑1 (MQ=255)
                                 acttGCGTGCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGGTGATAGCATGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGTTCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGc                                                                                                                      <  1:424111/148‑1 (MQ=255)
                                      cGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCActc                                                                                                                 <  2:1981478/150‑1 (MQ=255)
                                        ttCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTg                                                                                                               <  1:918178/150‑1 (MQ=255)
                                        ttCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTg                                                                                                               >  1:1706748/1‑150 (MQ=255)
                                        gtCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTg                                                                                                               <  2:481963/149‑1 (MQ=255)
                                         tCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGc                                                                                                              <  1:2031997/150‑1 (MQ=255)
                                         tCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGc                                                                                                              >  2:1819785/1‑150 (MQ=255)
                                           cACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGaaa                                                                                                            >  1:1392176/1‑150 (MQ=255)
                                           cACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCaa                                                                                                            >  1:1399559/1‑150 (MQ=255)
                                           cACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCaa                                                                                                            >  2:1806863/1‑150 (MQ=255)
                                                   gaagaaTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTAAGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATgg                                                                                                    >  2:1103856/1‑150 (MQ=255)
                                                      gACTATGGCAGGGAAGTTGCGCTGCATTTTGCTGGTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGt                                                                                                 <  1:1057111/150‑1 (MQ=255)
                                                          aTGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTc                                                                                             >  1:1709330/1‑150 (MQ=255)
                                                          aTGGCAGGGAAGTTACGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTc                                                                                             >  1:1323430/1‑150 (MQ=255)
                                                           tGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGTATGTTTGTATCATCTCTAAGTTATGCAGAAAAAACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCg                                                                                            >  1:1361505/1‑150 (MQ=255)
                                                                 ggAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTCTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCATTCTTATGAACAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCAGCAACCAGATGGGGTGCTCGACATCa                                                                                      >  2:700281/1‑150 (MQ=255)
                                                                 ggAAGGTTCGCTGCTTTTTGCTTTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCa                                                                                      <  2:1602322/150‑1 (MQ=255)
                                                                    aGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAg                                                                                   <  2:6287/150‑1 (MQ=255)
                                                                    aGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTCATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAg                                                                                   <  1:772964/150‑1 (MQ=255)
                                                                        tCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGTATCTCGCTCTTTGATGTTGAAGCCACTCTACAACCAGATGGGGTGCTCGACATCAAAGaaaa                                                                               <  1:1943850/150‑1 (MQ=255)
                                                                        tCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGaaaa                                                                               <  1:803280/150‑1 (MQ=255)
                                                                        tCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGaaaa                                                                               <  1:914437/150‑1 (MQ=255)
                                                                         cGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGATGACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAat                                                                              >  1:92141/1‑150 (MQ=255)
                                                                          gCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAata                                                                             >  2:1770133/1‑150 (MQ=255)
                                                                           cTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAatat                                                                            >  1:1278777/1‑150 (MQ=255)
                                                                             gCATTTTGCTGTTGATAGCAGGGCTGTTTGTTTCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAATTATTc                                                                          >  1:635925/1‑150 (MQ=255)
                                                                             gCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATAtta                                                                          >  1:529631/1‑149 (MQ=255)
                                                                              cATTTTGCTGTTGATAGAAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCa                                                                         >  1:559358/1‑150 (MQ=255)
                                                                                 tttGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCAttt                                                                      >  2:1594005/1‑150 (MQ=255)
                                                                                       gTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAATAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGgc                                                                <  1:1139732/150‑1 (MQ=255)
                                                                                       gTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGgc                                                                >  2:1262761/1‑150 (MQ=255)
                                                                                          gATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGcgcg                                                             <  1:666304/150‑1 (MQ=255)
                                                                                          gATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGcgcg                                                             <  2:185645/150‑1 (MQ=255)
                                                                                            tAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGaa                                                           <  2:249752/150‑1 (MQ=255)
                                                                                                 gggCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATcag                                                      <  1:1529295/150‑1 (MQ=255)
                                                                                                   gCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGTGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGAAGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATcagca                                                    <  1:1150051/150‑1 (MQ=255)
                                                                                                   gCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATcagca                                                    >  1:1716015/1‑150 (MQ=255)
                                                                                                        ttGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCCCTCTGCAACCAGATGGGGTGCTCGACATCTAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTa                                               >  1:127234/1‑150 (MQ=255)
                                                                                                          gTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAg                                             >  2:806230/1‑150 (MQ=255)
                                                                                                           tATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGc                                            <  1:1268366/150‑1 (MQ=255)
                                                                                                            atcatcTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCa                                           >  2:1572540/1‑150 (MQ=255)
                                                                                                              catcTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACg                                         >  1:598799/1‑150 (MQ=255)
                                                                                                               atcTCTAAGTTATGCAGAAAACACGTAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACgg                                        <  2:540476/150‑1 (MQ=255)
                                                                                                                tctcTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGc                                       >  1:1825997/1‑150 (MQ=255)
                                                                                                                 ctctAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCt                                      >  2:1788785/1‑150 (MQ=255)
                                                                                                                 ctctAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCt                                      >  2:1912537/1‑150 (MQ=255)
                                                                                                                   ctAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCttt                                    >  2:1043412/1‑150 (MQ=255)
                                                                                                                   ctAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGACACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGAAGATTAAGAACGTCttt                                    >  2:1851449/1‑150 (MQ=255)
                                                                                                                      aGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTAt                                 <  2:386931/150‑1 (MQ=255)
                                                                                                                        ttATGCAGAAAACACGGAGATCCCTTCTTATTAAGAATGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCg                               <  1:121678/150‑1 (MQ=255)
                                                                                                                          aTGCATAAAACACGGAGATCCGTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCAGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTg                             <  2:1368470/150‑1 (MQ=255)
                                                                                                                            gCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCCCGGCTTTTATCGTGAt                           >  2:107419/1‑150 (MQ=255)
                                                                                                                             cAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGAtt                          >  1:513556/1‑150 (MQ=255)
                                                                                                                              aGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGACACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGAttt                         >  2:1324283/1‑150 (MQ=255)
                                                                                                                                 aaaCACGGAGATCCCTTCTTCATAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTAcc                      <  1:770442/150‑1 (MQ=255)
                                                                                                                                   acacGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCAc                    <  2:1197935/150‑1 (MQ=255)
                                                                                                                                     agcgagaTCCCTTCTTCATAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACAACGa                  >  1:232658/4‑150 (MQ=255)
                                                                                                                                      gcgagaTCCCTTCTTCATAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGAc                 >  1:1802930/3‑150 (MQ=255)
                                                                                                                                      cGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGAc                 <  2:1361505/150‑1 (MQ=255)
                                                                                                                                      cGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGAc                 >  1:477650/1‑150 (MQ=255)
                                                                                                                                       ggAGATGCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACt                <  2:1667659/150‑1 (MQ=255)
                                                                                                                                        gagaTCCCTTCTTCATAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATTGGGTGCTCGACATCAAAGAAAATATTCATTAACAGGCGCGAAATCAGCAGATTACGCACGGCTTTTATCGTGATTTACCACGACtt               >  2:1411478/1‑149 (MQ=255)
                                                                                                                                        gagaTCCCTTCTTCATAAGAACGGATCTAGCTCTTTGATGTTGACGCCACTCTGCAACCAGATGGGGAGCTCGACATCAAAGAAAATATTCATTTTCACGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTa               >  2:198135/1‑150 (MQ=255)
                                                                                                                                           aTCCATTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGAGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATgg            >  1:626854/1‑150 (MQ=255)
                                                                                                                                               cttcttATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATGGATGc        >  2:1483153/1‑150 (MQ=255)
                                                                                                                                               cttcttATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGGGCTTTACCACGCCTATGGATGc        >  2:1929679/1‑150 (MQ=255)
                                                                                                                                                   ttATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATGGATGCAGcc    >  1:1444636/1‑150 (MQ=255)
                                                                                                                                                   ttATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATGGATGCAGcc    >  1:212118/1‑150 (MQ=255)
                                                                                                                                                    tATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGAGCTAAATCAGCAGATTAAGCAAGGATTTTATCGTGATTTACCACGACTATGGATGCAGCCt   >  1:406378/1‑150 (MQ=255)
                                                                                                                                                     aTGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATGGATGCAGCCTg  >  2:211444/1‑150 (MQ=255)
                                                                                                                                                     aTGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATGGATGCAGCCTg  >  1:2094876/1‑150 (MQ=255)
                                                                                                                                                     aTGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATGGATGCAGCCTg  <  1:243147/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATGGATGCAGCCTG  >  NC_000913/1324688‑1324986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: