Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,324,838 T→A 6.0% Y31* (TAT→TAA) ‡ yciQ → enhancer of membrane protein expression; putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,324,8380TA 6.0% 377.5 / 6.4 124Y31* (TAT→TAA) ‡yciQenhancer of membrane protein expression; putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (64/51);  new base A (6/2);  total (70/54)
Fisher's exact test for biased strand distribution p-value = 4.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 126 total aligned reads displayed.

GGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATGGATGCAGCCTGA  >  NC_000913/1324689‑1324987
                                                                                                                                                     |                                                                                                                                                     
  cGTTGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGTAGACTATTGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGAACCCTTCTTATga                                                                                                                                                     <  2:1122623/150‑1 (MQ=255)
   gTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGGTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATgaa                                                                                                                                                    <  2:765162/150‑1 (MQ=255)
      ggTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATgaagaa                                                                                                                                                 <  1:1714018/150‑1 (MQ=255)
         gATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAggg                                                                                                                                              <  2:877098/150‑1 (MQ=255)
          aTGTGAAGCCTTTCTTATACCAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGGTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGa                                                                                                                                             <  2:556518/150‑1 (MQ=255)
           tgtgAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTATGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGaa                                                                                                                                            >  2:2136745/1‑149 (MQ=255)
            gtgACGCCTGTCTTATAACACATTGCGTTCCCCGGATGGCAGACTAAGGCAGGGAAGGTTCGCTGCATTTGGCTGGTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTGCATAAGAAGGGAtc                                                                                                                                           <  2:424275/150‑1 (MQ=255)
            gtgAAGCCTTTCTTATAACAAATTGCGTTCCACGTATGGAAGACTATGGCAGGGAAGTTTCGCTTCATTTTGATGTTTATAGCAGTGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACTGAGATCCCTTCTTATGAAGTATGGAta                                                                                                                                           >  1:370670/1‑149 (MQ=255)
             tgAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGAtct                                                                                                                                          >  2:842041/1‑150 (MQ=255)
               aaGCCTTTATTATAAAAAATTGCGTTCAACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATATTGCTGTTGATAGCAGGGCTGTATGTATAATCTCTAAGTTATGCAGAAAACACGGAGATCCATTCTTATCAAGAAGTGaaatac                                                                                                                                        >  1:598224/1‑145 (MQ=255)
                aGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGc                                                                                                                                       <  2:1200031/150‑1 (MQ=255)
                  ccTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGctc                                                                                                                                     <  2:58425/150‑1 (MQ=255)
                   cTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGctct                                                                                                                                    >  2:2112006/1‑150 (MQ=255)
                     ttCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCttt                                                                                                                                  <  1:896893/150‑1 (MQ=255)
                      tCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTg                                                                                                                                 >  1:188192/1‑150 (MQ=255)
                       cTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATAATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGa                                                                                                                                >  2:470199/1‑150 (MQ=255)
                       cTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGGTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGa                                                                                                                                <  2:1346094/150‑1 (MQ=255)
                       attATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGa                                                                                                                                <  1:1566116/149‑1 (MQ=255)
                         tataACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATg                                                                                                                              >  2:1324206/1‑150 (MQ=255)
                         tataACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATg                                                                                                                              <  1:35336/150‑1 (MQ=255)
                          ataACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTAGATGt                                                                                                                             >  1:2104379/1‑150 (MQ=255)
                           taACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGtt                                                                                                                            >  1:185645/1‑150 (MQ=255)
                           taACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGtt                                                                                                                            >  1:1887458/1‑150 (MQ=255)
                           taACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGtt                                                                                                                            >  2:1267749/1‑150 (MQ=255)
                           taACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGtt                                                                                                                            >  1:249779/1‑150 (MQ=255)
                           taACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGtt                                                                                                                            <  2:18989/150‑1 (MQ=255)
                            aaCAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTg                                                                                                                           >  2:1531602/1‑150 (MQ=255)
                              cAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGaa                                                                                                                         <  1:13910/150‑1 (MQ=255)
                                acttGCGTGCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGGTGATAGCATGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGTTCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGc                                                                                                                       <  1:424111/148‑1 (MQ=255)
                                    gCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCAct                                                                                                                   >  1:2086927/1‑150 (MQ=255)
                                       ttCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTg                                                                                                                >  1:1706748/1‑150 (MQ=255)
                                        tCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGc                                                                                                               <  1:2031997/150‑1 (MQ=255)
                                          cACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGaaa                                                                                                             >  1:1392176/1‑150 (MQ=255)
                                          cACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCaa                                                                                                             >  1:1399559/1‑150 (MQ=255)
                                          cACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCaa                                                                                                             >  2:1806863/1‑150 (MQ=255)
                                                  gAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATgg                                                                                                     >  2:595633/1‑150 (MQ=255)
                                                  gAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTGGTATCATCTCTACGTTATGCAGAAAACACGGAGATCCCTTCTTAGGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATgg                                                                                                     <  1:220605/150‑1 (MQ=255)
                                                    aGACTTTGGCAGGGAATTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATgggg                                                                                                   <  1:513066/150‑1 (MQ=255)
                                                     gACTATGGCAGGGAAGTTGCGCTGCATTTTGCTGGTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGt                                                                                                  <  1:1057111/150‑1 (MQ=255)
                                                         aTGGCAGGGAAGTTACGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTc                                                                                              >  1:1323430/1‑150 (MQ=255)
                                                          tGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGTATGTTTGTATCATCTCTAAGTTATGCAGAAAAAACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCg                                                                                             >  1:1361505/1‑150 (MQ=255)
                                                             cAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACa                                                                                          <  2:968683/150‑1 (MQ=255)
                                                                ggAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTCTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCATTCTTATGAACAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCAGCAACCAGATGGGGTGCTCGACATCa                                                                                       >  2:700281/1‑150 (MQ=255)
                                                                ggAAGGTTCGCTGCTTTTTGCTTTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCa                                                                                       <  2:1602322/150‑1 (MQ=255)
                                                                   aGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAg                                                                                    <  2:6287/150‑1 (MQ=255)
                                                                   aGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTCATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAg                                                                                    <  1:772964/150‑1 (MQ=255)
                                                                       tCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGTATCTCGCTCTTTGATGTTGAAGCCACTCTACAACCAGATGGGGTGCTCGACATCAAAGaaaa                                                                                <  1:1943850/150‑1 (MQ=255)
                                                                       tCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGaaaa                                                                                <  1:914437/150‑1 (MQ=255)
                                                                        cGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGATGACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAat                                                                               >  1:92141/1‑150 (MQ=255)
                                                                         gCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAata                                                                              >  2:1770133/1‑150 (MQ=255)
                                                                          cTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAatat                                                                             >  1:1278777/1‑150 (MQ=255)
                                                                             cATTTTGCTGTTGATAGAAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCa                                                                          >  1:559358/1‑150 (MQ=255)
                                                                              ttttaGCTGAAGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATTCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCAt                                                                         <  1:369748/149‑1 (MQ=255)
                                                                                tttGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCAttt                                                                       >  2:1594005/1‑150 (MQ=255)
                                                                                tttGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTCCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCAttt                                                                       <  2:1181221/150‑1 (MQ=255)
                                                                                      gTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAACACACGGAGATCCCTTCTGATGAAGAAGGGCTCTCGCTCTTTGATGTTGAAGCCACTCTGCGACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAggg                                                                 >  2:2066125/1‑149 (MQ=255)
                                                                                      gTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAATAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGgc                                                                 <  1:1139732/150‑1 (MQ=255)
                                                                                      gTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGgc                                                                 >  2:1262761/1‑150 (MQ=255)
                                                                                         gATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGcgcg                                                              <  2:185645/150‑1 (MQ=255)
                                                                                         gATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGcgcg                                                              <  1:666304/150‑1 (MQ=255)
                                                                                           tAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGaa                                                            <  2:249752/150‑1 (MQ=255)
                                                                                                gggCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATcag                                                       >  2:1820578/1‑150 (MQ=255)
                                                                                                gggCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATcag                                                       <  1:1529295/150‑1 (MQ=255)
                                                                                                  gCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGTGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGAAGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATcagca                                                     <  1:1150051/150‑1 (MQ=255)
                                                                                                  gCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATcagca                                                     >  1:1716015/1‑150 (MQ=255)
                                                                                                       ttGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCCCTCTGCAACCAGATGGGGTGCTCGACATCTAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTa                                                >  1:127234/1‑150 (MQ=255)
                                                                                                          tATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGc                                             <  1:1268366/150‑1 (MQ=255)
                                                                                                           atcatcTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCa                                            >  2:1572540/1‑150 (MQ=255)
                                                                                                             catcTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACg                                          >  1:598799/1‑150 (MQ=255)
                                                                                                              atcTCTAAGTTATGCAGAAAACACGTAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACgg                                         <  2:540476/150‑1 (MQ=255)
                                                                                                                ctctAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCt                                       >  2:1788785/1‑150 (MQ=255)
                                                                                                                ctctAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCt                                       >  2:1912537/1‑150 (MQ=255)
                                                                                                                  ctAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCttt                                     >  2:1043412/1‑150 (MQ=255)
                                                                                                                  ctAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGACACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGAAGATTAAGAACGTCttt                                     >  2:1851449/1‑150 (MQ=255)
                                                                                                                     aGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTAt                                  <  2:386931/150‑1 (MQ=255)
                                                                                                                       ttATGCAGAAAACACGGAGATCCCTTCTTATTAAGAATGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCg                                <  1:121678/150‑1 (MQ=255)
                                                                                                                         aTGCATAAAACACGGAGATCCGTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCAGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTg                              <  2:1368470/150‑1 (MQ=255)
                                                                                                                           gCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCCCGGCTTTTATCGTGAt                            >  2:107419/1‑150 (MQ=255)
                                                                                                                           gCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGAt                            >  2:1685990/1‑150 (MQ=255)
                                                                                                                            cAGAAAACACGGAGATCCCTTCTTCATAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGAtt                           >  2:770442/1‑150 (MQ=255)
                                                                                                                            cAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGAtt                           >  1:513556/1‑150 (MQ=255)
                                                                                                                             aGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGACACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGAttt                          >  2:1324283/1‑150 (MQ=255)
                                                                                                                              gAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTa                         >  1:908054/1‑150 (MQ=255)
                                                                                                                               aaaaCACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTAc                        >  1:1725850/1‑150 (MQ=255)
                                                                                                                                aaaCACGGAGATCCCTTCTTCATAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTAcc                       <  1:770442/150‑1 (MQ=255)
                                                                                                                                  acacGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCAc                     <  2:1197935/150‑1 (MQ=255)
                                                                                                                                    agcgagaTCCCTTCTTCATAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACAACGa                   >  1:232658/4‑150 (MQ=255)
                                                                                                                                     gcgagaTCCCTTCTTCATAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGAc                  >  1:1802930/3‑150 (MQ=255)
                                                                                                                                     cGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGAc                  >  1:687383/1‑150 (MQ=255)
                                                                                                                                     cGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGAc                  >  1:477650/1‑150 (MQ=255)
                                                                                                                                     cGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGAc                  <  2:1361505/150‑1 (MQ=255)
                                                                                                                                       gagaTCCCTTCTTCATAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATTGGGTGCTCGACATCAAAGAAAATATTCATTAACAGGCGCGAAATCAGCAGATTACGCACGGCTTTTATCGTGATTTACCACGACtt                >  2:1411478/1‑149 (MQ=255)
                                                                                                                                       gagaTCCCTTCTTCATAAGAACGGATCTAGCTCTTTGATGTTGACGCCACTCTGCAACCAGATGGGGAGCTCGACATCAAAGAAAATATTCATTTTCACGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTa                >  2:198135/1‑150 (MQ=255)
                                                                                                                                          aTCCATTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGAGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATgg             >  1:626854/1‑150 (MQ=255)
                                                                                                                                           tCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATGGa            <  1:969376/150‑1 (MQ=255)
                                                                                                                                              cttcttATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGGGCTTTACCACGCCTATGGATGc         >  2:1929679/1‑150 (MQ=255)
                                                                                                                                                  ttATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATGGATGCAGcc     >  1:212118/1‑150 (MQ=255)
                                                                                                                                                  ttATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATGGATGCAGcc     >  1:1444636/1‑150 (MQ=255)
                                                                                                                                                    aTGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATGGATGCAGCCTg   >  1:2094876/1‑150 (MQ=255)
                                                                                                                                                    aTGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATGGATGCAGCCTg   >  2:211444/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GGCGTAGGTGATGTGAAGCCTTTCTTATAACAAATTGCGTTCCACGGATGGAAGACTATGGCAGGGAAGTTTCGCTGCATTTTGCTGTTGATAGCAGGGCTGTTTGTATCATCTCTAAGTTATGCAGAAAACACGGAGATCCCTTCTTATGAAGAAGGGATCTCGCTCTTTGATGTTGAAGCCACTCTGCAACCAGATGGGGTGCTCGACATCAAAGAAAATATTCATTTTCAGGCGCGAAATCAGCAGATTAAGCACGGCTTTTATCGTGATTTACCACGACTATGGATGCAGCCTGA  >  NC_000913/1324689‑1324987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: