Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,462,028 G→A 5.3% G379D (GGT→GAT)  paaJ → 3‑oxoadipyl‑CoA/3‑oxo‑5,6‑dehydrosuberyl‑CoA thiolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,462,0280GA 5.3% 489.3 / 7.0 149G379D (GGT→GAT) paaJ3‑oxoadipyl‑CoA/3‑oxo‑5,6‑dehydrosuberyl‑CoA thiolase
Reads supporting (aligned to +/- strand):  ref base G (76/65);  new base A (3/5);  total (79/70)
Fisher's exact test for biased strand distribution p-value = 4.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 151 total aligned reads displayed.

GGCGTTGGGTGTACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTGAAACCGCGTCTGTTGATGAGT  >  NC_000913/1461879‑1462176
                                                                                                                                                     |                                                                                                                                                    
     tGGGTGTACTACGCGAATTGGGGCTGCCGGATGATGCCCCACATGTTAACCCCACCGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGtt                                                                                                                                                 <  1:1548998/150‑1 (MQ=255)
      gggTGTACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTa                                                                                                                                                >  1:847387/1‑150 (MQ=255)
        gtgtACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACg                                                                                                                                              >  2:388410/1‑150 (MQ=255)
          gtACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGACCGTTACGcc                                                                                                                                            <  2:157095/150‑2 (MQ=255)
          gtACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGACCGTTACGcc                                                                                                                                            <  1:1170822/150‑2 (MQ=255)
             ctacGCGAATTGTGGATGCCTGATGATGCCCCACATGTTAACCCCATCGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATtg                                                                                                                                         <  2:114434/150‑1 (MQ=255)
              tacGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATtgt                                                                                                                                        >  1:1054022/1‑150 (MQ=255)
               acGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTAACCCCCACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATtgtg                                                                                                                                       <  1:1326232/150‑1 (MQ=255)
                  cgAATTGGGGCTGCCTGATGATGCCCCACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCAc                                                                                                                                    >  2:1044650/1‑150 (MQ=255)
                       tGGGGCTGCCTGATGATGCCCCACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCACCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGGACCAtgt                                                                                                                               <  1:1618094/150‑1 (MQ=255)
                        ggggCTGCCTGATGATGCCCCACATGTTAGCCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCGGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCAtgtg                                                                                                                              <  2:2059588/150‑1 (MQ=255)
                        ggggCTGCCTGATGATGCCCCACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCAtgtg                                                                                                                              >  2:1112188/1‑150 (MQ=255)
                         gggCTGCCTGATGATGCCCCACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGc                                                                                                                             >  1:764210/1‑150 (MQ=255)
                          ggCTGCCTGATGATGCCCCACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGGCATGAGCTGCATCGGCGTAACGGTGGTTACGGATTGTGCACCATGTGCa                                                                                                                            >  2:410998/1‑150 (MQ=255)
                          ggCTGCCTGATGATGCCCCACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCa                                                                                                                            >  1:297207/1‑150 (MQ=255)
                              gCCTGATGATGCCCCACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCCCCATGTGCATCgg                                                                                                                        >  1:1522584/1‑150 (MQ=255)
                              gCCTGATGATGCCCCACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCgg                                                                                                                        <  1:715076/150‑1 (MQ=255)
                                   atgatgCCCCACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCg                                                                                                                   >  2:635171/1‑150 (MQ=255)
                                    tgatgCCCCACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCgg                                                                                                                  >  2:1052567/1‑150 (MQ=255)
                                        gCCCCACATGTTAACCCCAACGGAGGTGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAg                                                                                                              >  2:2087111/1‑150 (MQ=255)
                                        gCCCCACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCGTCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAg                                                                                                              >  2:1924454/1‑150 (MQ=255)
                                        gCCCCACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAg                                                                                                              <  1:458396/150‑1 (MQ=255)
                                          cccACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAggg                                                                                                            <  1:262780/150‑1 (MQ=255)
                                          cccACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAggg                                                                                                            >  1:1371078/1‑150 (MQ=255)
                                           ccACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGc                                                                                                           <  1:1931676/150‑1 (MQ=255)
                                                tGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGc                                                                                                      >  2:120644/1‑150 (MQ=255)
                                                  ttAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCa                                                                                                    >  1:170977/1‑150 (MQ=255)
                                                  ttAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCa                                                                                                    <  1:1016507/150‑1 (MQ=255)
                                                  ttAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCa                                                                                                    >  1:1820442/1‑150 (MQ=255)
                                                    aaCCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATg                                                                                                  <  2:1871933/150‑1 (MQ=255)
                                                      ccccAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGAt                                                                                                >  1:1726561/1‑150 (MQ=255)
                                                        ccAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTc                                                                                              <  1:882564/150‑1 (MQ=255)
                                                        ccAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTc                                                                                              >  1:663603/1‑150 (MQ=255)
                                                          aaCGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTg                                                                                            <  1:635171/150‑1 (MQ=255)
                                                             ggAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAg                                                                                         <  1:1585618/150‑1 (MQ=255)
                                                              gAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGc                                                                                        >  2:655420/1‑150 (MQ=255)
                                                                     tATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACGGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTg                                                                                 <  1:644391/150‑1 (MQ=255)
                                                                      aTCGGCTTAGGCCACCCGGTGGGAATGCGTGGTGCCCGCCGGGCACTGGCTGCCAGCCATGAGCGTCATCTGCGGAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCTGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGa                                                                                <  1:449936/150‑1 (MQ=255)
                                                                       tCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAg                                                                               <  1:1670365/150‑1 (MQ=255)
                                                                        cGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGc                                                                              <  2:2096005/150‑1 (MQ=255)
                                                                        cGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGc                                                                              <  2:621752/150‑1 (MQ=255)
                                                                        cGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGc                                                                              <  1:177481/150‑1 (MQ=255)
                                                                         gCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAAAGGTCGTTACGCATTGTGCACCATGTGCATCGGTCTCGGTCAGAGCATCTCCATGATTCTGGAGCGTGTTTGAGCa                                                                             >  1:2130028/1‑150 (MQ=255)
                                                                          ccTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGACATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGAGCATCGGAGTCGGTCAGGGCATCGCCAAGATTCTGGAGCGTGTTTGAGCat                                                                            >  2:2204441/1‑150 (MQ=255)
                                                                             tAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTTGAGCGTGTTTGAGCATATc                                                                         >  2:597245/1‑150 (MQ=255)
                                                                              aGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCa                                                                        >  2:970912/1‑150 (MQ=255)
                                                                                gCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAAc                                                                      <  1:1363838/150‑1 (MQ=255)
                                                                                gCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGGGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGCGCGTGTTTGAGCATATCacc                                                                      >  2:220711/1‑148 (MQ=255)
                                                                                 ccATCCGGTGGGAATGAGTGGTACCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGGCGGGCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAAcc                                                                     <  1:1372610/150‑1 (MQ=255)
                                                                                       gTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAg                                                               <  1:1466265/150‑1 (MQ=255)
                                                                                        gtGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGt                                                              <  2:117046/149‑1 (MQ=255)
                                                                                           ggAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCGATGCTTCTGGAGCGTGTTTGAGCATAGCTACCTGCGAGTAcc                                                           >  1:126371/1‑150 (MQ=255)
                                                                                            gAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTAccc                                                          <  2:83021/150‑1 (MQ=255)
                                                                                             aaTGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTAcccg                                                         >  1:1842557/1‑149 (MQ=255)
                                                                                                    ggtgCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTAGGTCAGGGCTTCGCCATGAGTCTGGAGCGTGTTTGAGCATATGACCCTGCGAGTACACTACATTGa                                                  >  1:412815/1‑150 (MQ=255)
                                                                                                     gtgCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGAt                                                 <  2:162232/150‑1 (MQ=255)
                                                                                                        cccGCCTGGCACTGGCTGCCAGCCCTGAGCGGCCGCGGCGTCACGATCCTGACGCATTGGTCCCCATGGGCATCGGTGTCGCGAAGGGCTTCGCCAGGAGTCTTGAGCGTGTTGGAGAATATCCACCTGCGAGTACCCAACAAGGAGAAc                                              >  2:352270/1‑150 (MQ=255)
                                                                                                                gCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACaa                                      <  2:380864/150‑1 (MQ=255)
                                                                                                                 cACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCAGATCAACCTGCGAGTACCCTACAATGATACCCAAGACaaa                                     >  2:2023680/1‑150 (MQ=255)
                                                                                                                  aCTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAg                                    <  1:968974/150‑1 (MQ=255)
                                                                                                                     ggCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATTACCAATACAAAGCtt                                 >  2:259185/1‑150 (MQ=255)
                                                                                                                       cTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGa                               >  2:81638/1‑150 (MQ=255)
                                                                                                                        tGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCATGAc                              >  2:1452459/1‑150 (MQ=255)
                                                                                                                        ggccagccaTGAGCTGCATCGGGGTACCGGTCGTTACGAATGGGGCACCATGTGCAGCGGGGCCGGGCCGGGCATCGCAATGATTCTGGAGCGGGTTTTAGAATATAAACCTGCGATAACCCTAAAATTAAAACCAATACAAAGCTTGAc                              <  2:1607606/149‑1 (MQ=255)
                                                                                                                         gccagccaTGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATTCAAAGCTTGAcc                             >  1:274890/1‑150 (MQ=255)
                                                                                                                         gccagccaTGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGAcc                             <  2:1045058/150‑1 (MQ=255)
                                                                                                                         gccagccaTGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGAcc                             >  1:1628497/1‑150 (MQ=255)
                                                                                                                         gccagccaTGAGCTGCATCGGCGTAACGGTCGTTACGAATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGAcc                             <  1:388410/150‑1 (MQ=255)
                                                                                                                          ccagccaTGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGAccc                            >  2:614627/1‑150 (MQ=255)
                                                                                                                           cagccaTGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATaa                                               >  2:1242679/1‑130 (MQ=255)
                                                                                                                           cagccaTGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATaa                                               <  1:1242679/130‑1 (MQ=255)
                                                                                                                             gccaTGTGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGAt                         <  2:1111677/150‑1 (MQ=255)
                                                                                                                             gccaTGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGAt                         <  2:764210/150‑1 (MQ=255)
                                                                                                                              ccaTGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGAtt                        <  1:717592/150‑1 (MQ=255)
                                                                                                                              ccaTGAGCTGCATCGGCGTAACGGTCGTTACGCATTGAGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGAtt                        <  1:1052567/150‑1 (MQ=255)
                                                                                                                               caTGATCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTg                       <  1:1650861/150‑1 (MQ=255)
                                                                                                                               caTGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCCACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTg                       >  1:1212844/1‑150 (MQ=255)
                                                                                                                               caTGAGAGGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTg                       <  2:451408/150‑1 (MQ=255)
                                                                                                                                aTGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGACATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTGa                      <  1:197693/150‑1 (MQ=255)
                                                                                                                                   aGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATTTCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTGAAAc                   >  1:1480798/1‑150 (MQ=255)
                                                                                                                                   aGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTGAAAc                   <  1:2016743/150‑1 (MQ=255)
                                                                                                                                    gCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTGAAAcc                  >  2:727615/1‑150 (MQ=255)
                                                                                                                                    gCTGCATCGGCGTAACGGTCGATACGCATTGTGCCCCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCTATTGAAAcc                  >  2:1207439/1‑150 (MQ=255)
                                                                                                                                      tGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTGAAACcgc                >  1:69378/1‑150 (MQ=255)
                                                                                                                                       gCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTGAAACcgcg               <  1:1044650/150‑1 (MQ=255)
                                                                                                                                       gCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTGAAACcgcg               <  2:1626422/150‑1 (MQ=255)
                                                                                                                                       gCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTGAAACcgcg               <  2:170977/150‑1 (MQ=255)
                                                                                                                                       cacaatgCGTAACGACCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGTGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTATAATGATAACCAATACAAAGCTTGACCCGATTGAAACcgcg               >  1:269993/7‑150 (MQ=255)
                                                                                                                                         atgggCGTAACGGTCGTTACGTTTTGGGGAAGATTTGAATCGGTGTCGTTCAGGGCATCGCAATGATTCTGGAGCGTGTTTGAGCATATCTACCTGCGAGTACCCTACAATGATATCCATTACAAAGCTTGACCCGATTGAAACCGCGTc             <  1:1436057/147‑1 (MQ=255)
                                                                                                                                         aTCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTGAAACCGCGTc             <  2:847387/150‑1 (MQ=255)
                                                                                                                                          tCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTGAAACCGCGTCt            >  1:750313/1‑150 (MQ=255)
                                                                                                                                           atgCGTAACGACCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTGAAACCGCGTCTg           >  1:1441836/3‑150 (MQ=255)
                                                                                                                                            ggCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATACCCAATACAAAGCTTGACCCGATTGAAACCGCGTCTGt          <  2:254046/150‑1 (MQ=255)
                                                                                                                                             gCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTGAAACCGCGTCTGtt         <  1:1924454/150‑1 (MQ=255)
                                                                                                                                                 aaCGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGTAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTGAAACCGCGTCTGTtgatg     <  2:1054022/150‑1 (MQ=255)
                                                                                                                                                   cGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGCGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTGAAACCGCGTCTGTTGATGAg   >  2:1899021/1‑150 (MQ=255)
                                                                                                                                                   cGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTGAACCCGCGTCTGTTGATGAg   >  2:468388/1‑150 (MQ=255)
                                                                                                                                                   cGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTGAAACCGCGTCTGTTGATGAg   >  1:2129394/1‑150 (MQ=255)
                                                                                                                                                   cGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTGAAACCGCGTCTGTTGATGAg   >  2:885578/1‑150 (MQ=255)
                                                                                                                                                    ggTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTGAAACCGCGTCTGTTGATGAGt  <  2:705949/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GGCGTTGGGTGTACTACGCGAATTGGGGCTGCCTGATGATGCCCCACATGTTAACCCCAACGGAGGCGCTATCGCCTTAGGCCATCCGTTGGGAATGAGTGGTGCCCGCCTGGCACTGGCTGCCAGCCATGAGCTGCATCGGCGTAACGGTCGTTACGCATTGTGCACCATGTGCATCGGTGTCGGTCAGGGCATCGCCATGATTCTGGAGCGTGTTTGAGCATATCAACCTGCGAGTACCCTACAATGATAACCAATACAAAGCTTGACCCGATTGAAACCGCGTCTGTTGATGAGT  >  NC_000913/1461879‑1462176

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: